BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00025591
         (1933 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI31610.3| unnamed protein product [Vitis vinifera]              711   0.0  
ref|XP_002277686.2| PREDICTED: folylpolyglutamate synthase, mito...   701   0.0  
ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricin...   686   0.0  
ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichoc...   684   0.0  
ref|XP_002316257.1| folylpolyglutamate synthase [Populus trichoc...   671   0.0  

>emb|CBI31610.3| unnamed protein product [Vitis vinifera]
          Length = 618

 Score =  711 bits (1834), Expect = 0.0
 Identities = 366/518 (70%), Positives = 416/518 (80%), Gaps = 17/518 (3%)
 Frame = +3

Query: 141  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAIPKMKIIHVAGTKGKGSTCTFTESILRNC 320
            ITKRSR DKSNK   FD+L+DY+K+L+LDE I KMKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 108  ITKRSRGDKSNKRVLFDVLFDYLKMLDLDEPISKMKIIHVAGTKGKGSTCTFTESILRNC 167

Query: 321  GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLVYFWWCFNKLKETTTEDVPMPTXXXXXX 500
            GFRTGLFTSPHLID+RERFRLDG+EI ++KFL YFWWC+++LKE T EDVPMPT      
Sbjct: 168  GFRTGLFTSPHLIDVRERFRLDGVEICEEKFLAYFWWCWDRLKEKTDEDVPMPTFFHFIA 227

Query: 501  XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIVCGISSLGFDHMEILGNTLGEIAGE 680
                     EQVDV ILEVGLGG  DATNVVQEPIVCGI+SLG+DHMEILG+TLGEIAGE
Sbjct: 228  LLAFKIFAAEQVDVVILEVGLGGRLDATNVVQEPIVCGITSLGYDHMEILGHTLGEIAGE 287

Query: 681  KAGIFKQGVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYL 860
            KAGIFK+GVPAFTVPQPEEAM VLE KASQL+VPLQV  P+D SLLNGL+LGL GEHQYL
Sbjct: 288  KAGIFKRGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDASLLNGLKLGLEGEHQYL 347

Query: 861  NASLAIALCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTD--LYMDRP 1034
            NA LAIALC TWLQ+T HLE  YL+QTSSLPEQF++GL   S +GRAQIV D  + ++ P
Sbjct: 348  NAGLAIALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQIVPDRCINIESP 407

Query: 1035 GDLVFYLDGAHSPESMDMCGRWFSVATREDVQQH----------NGSKERLQADL----R 1172
            GDLVFYLDGAHSPESM++C RWFS+A +ED QQ             S+E +Q D     R
Sbjct: 408  GDLVFYLDGAHSPESMEICARWFSLAIKEDDQQPTLNYQPQDDVGSSRELVQKDYHGTSR 467

Query: 1173 KDSTKVLLFNCMPVRDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDP 1352
            K++T++LLFNCM VRDP LLLP L+N CA HGVHF++ALFVPN SVY++VGSHALPL D 
Sbjct: 468  KNTTQILLFNCMSVRDPHLLLPHLMNTCASHGVHFKQALFVPNISVYNRVGSHALPLTDL 527

Query: 1353 QVDLSWQLTLQRVWENLVCGDKAIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLP 1529
            QVDLSWQLTLQR+WE+L      I GGD K  D  REE  +D   S+ S E S+VF SLP
Sbjct: 528  QVDLSWQLTLQRLWESL------IHGGDAKNID-TREEGKDDTEKSVISCENSTVFPSLP 580

Query: 1530 SAIKWLRDSAQQNRSVRFQVLVTGSLHLVGDVLRLIKK 1643
             AIKWLRDS +QN+SVRFQVLVTGSLHLVGDVLRL+KK
Sbjct: 581  LAIKWLRDSVRQNQSVRFQVLVTGSLHLVGDVLRLVKK 618


>ref|XP_002277686.2| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Vitis
            vinifera]
          Length = 521

 Score =  701 bits (1810), Expect = 0.0
 Identities = 359/504 (71%), Positives = 408/504 (80%), Gaps = 3/504 (0%)
 Frame = +3

Query: 141  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAIPKMKIIHVAGTKGKGSTCTFTESILRNC 320
            ITKRSR DKSNK   FD+L+DY+K+L+LDE I KMKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 27   ITKRSRGDKSNKRVLFDVLFDYLKMLDLDEPISKMKIIHVAGTKGKGSTCTFTESILRNC 86

Query: 321  GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLVYFWWCFNKLKETTTEDVPMPTXXXXXX 500
            GFRTGLFTSPHLID+RERFRLDG+EI ++KFL YFWWC+++LKE T EDVPMPT      
Sbjct: 87   GFRTGLFTSPHLIDVRERFRLDGVEICEEKFLAYFWWCWDRLKEKTDEDVPMPTFFHFIA 146

Query: 501  XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIVCGISSLGFDHMEILGNTLGEIAGE 680
                     EQVDV ILEVGLGG  DATNVVQEPIVCGI+SLG+DHMEILG+TLGEIAGE
Sbjct: 147  LLAFKIFAAEQVDVVILEVGLGGRLDATNVVQEPIVCGITSLGYDHMEILGHTLGEIAGE 206

Query: 681  KAGIFKQGVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYL 860
            KAGIFK+GVPAFTVPQPEEAM VLE KASQL+VPLQV  P+D SLLNGL+LGL GEHQYL
Sbjct: 207  KAGIFKRGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDASLLNGLKLGLEGEHQYL 266

Query: 861  NASLAIALCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTD--LYMDRP 1034
            NA LAIALC TWLQ+T HLE  YL+QTSSLPEQF++GL   S +GRAQIV D  + ++ P
Sbjct: 267  NAGLAIALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQIVPDRCINIESP 326

Query: 1035 GDLVFYLDGAHSPESMDMCGRWFSVATREDVQQHNGSKERLQADLRKDSTKVLLFNCMPV 1214
            GDLVFYLDGAHSPESM++C RWFS+A +ED QQ   + +  Q D+      +LLFNCM V
Sbjct: 327  GDLVFYLDGAHSPESMEICARWFSLAIKEDDQQPTLNYQP-QDDVGSSRELILLFNCMSV 385

Query: 1215 RDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDPQVDLSWQLTLQRVW 1394
            RDP LLLP L+N CA HGVHF++ALFVPN SVY++VGSHALPL D QVDLSWQLTLQR+W
Sbjct: 386  RDPHLLLPHLMNTCASHGVHFKQALFVPNISVYNRVGSHALPLTDLQVDLSWQLTLQRLW 445

Query: 1395 ENLVCGDKAIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLPSAIKWLRDSAQQNR 1571
            E+L+        GD K  D  REE  +D   S+ S E S+VF SLP AIKWLRDS +QN+
Sbjct: 446  ESLI-------HGDAKNID-TREEGKDDTEKSVISCENSTVFPSLPLAIKWLRDSVRQNQ 497

Query: 1572 SVRFQVLVTGSLHLVGDVLRLIKK 1643
            SVRFQVLVTGSLHLVGDVLRL+KK
Sbjct: 498  SVRFQVLVTGSLHLVGDVLRLVKK 521


>ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricinus communis]
            gi|223536513|gb|EEF38160.1| folylpolyglutamate synthase,
            putative [Ricinus communis]
          Length = 531

 Score =  686 bits (1770), Expect = 0.0
 Identities = 352/508 (69%), Positives = 407/508 (80%), Gaps = 7/508 (1%)
 Frame = +3

Query: 141  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAIPKMKIIHVAGTKGKGSTCTFTESILRNC 320
            ITKRSRADKSNKGDRFD L+DY+K+L+L++AI +MKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 27   ITKRSRADKSNKGDRFDFLFDYLKMLDLEQAISEMKIIHVAGTKGKGSTCTFTESILRNC 86

Query: 321  GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLVYFWWCFNKLKETTTEDVPMPTXXXXXX 500
            GFRTGLFTSPHLID+RERFRLDG +I ++KFL YFWWC+++LKE TTE++PMPT      
Sbjct: 87   GFRTGLFTSPHLIDVRERFRLDGEDICEEKFLAYFWWCYDRLKEKTTEEIPMPTYFRFLA 146

Query: 501  XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIVCGISSLGFDHMEILGNTLGEIAGE 680
                     EQVDVAILEVGLGG FDATNVVQ PIVCGISSLG+DHMEILGNTLGEIAGE
Sbjct: 147  LLAFKIFAAEQVDVAILEVGLGGKFDATNVVQTPIVCGISSLGYDHMEILGNTLGEIAGE 206

Query: 681  KAGIFKQGVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYL 860
            KAGIFK  +PAFTVPQ +EAM VLE KAS+L+VPLQVA PLD SLLNGL+LGL GEHQYL
Sbjct: 207  KAGIFKHRIPAFTVPQLDEAMHVLEDKASKLDVPLQVAPPLDPSLLNGLKLGLEGEHQYL 266

Query: 861  NASLAIALCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTD--LYMDRP 1034
            NA LAIAL   WLQ+TGH+E  YL+Q+S LPE F+RGL     +GRAQIV D  +  +  
Sbjct: 267  NAGLAIALSSKWLQRTGHVEFSYLEQSSPLPEHFIRGLTTACLQGRAQIVPDRCVNTETN 326

Query: 1035 GDLVFYLDGAHSPESMDMCGRWFSVATREDVQQH--NGSKER-LQADLRKD-STKVLLFN 1202
            GDLVFYLDGAHSPESM+ C RWFS+A ++D QQH  N    R  ++ L    S ++LLFN
Sbjct: 327  GDLVFYLDGAHSPESMEACARWFSLAIKDDNQQHTLNSPPHRNYESTLGFHLSMQILLFN 386

Query: 1203 CMPVRDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDPQVDLSWQLTL 1382
            CM VRDPQLLLPRL+  CA  GV+F+KALFVPN SVY+KVGS ALP  DPQVDLSWQ TL
Sbjct: 387  CMSVRDPQLLLPRLMKTCASQGVYFKKALFVPNTSVYYKVGSQALPPVDPQVDLSWQFTL 446

Query: 1383 QRVWENLVCGDKAIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLPSAIKWLRDSA 1559
            QR+WE+LV GDK   GG+    D + EE  ++   ++++ E S+VF SLP AIKWLRDS 
Sbjct: 447  QRLWESLVRGDK---GGEANNVDALCEEVKDETGATVKNCENSAVFPSLPLAIKWLRDSV 503

Query: 1560 QQNRSVRFQVLVTGSLHLVGDVLRLIKK 1643
             QNRSVR QVLVTGSLHLVGDVLR++KK
Sbjct: 504  LQNRSVRIQVLVTGSLHLVGDVLRIVKK 531


>ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222851012|gb|EEE88559.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 539

 Score =  684 bits (1766), Expect = 0.0
 Identities = 354/518 (68%), Positives = 411/518 (79%), Gaps = 17/518 (3%)
 Frame = +3

Query: 141  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAIPKMKIIHVAGTKGKGSTCTFTESILRNC 320
            ITKRSRADKSN GDRFD+L+DY+K   L+EAI +MKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 28   ITKRSRADKSNNGDRFDVLFDYLK---LEEAISEMKIIHVAGTKGKGSTCTFTESILRNC 84

Query: 321  GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLVYFWWCFNKLKETTTEDVPMPTXXXXXX 500
            GFRTGLFTSPHLID+RERFRLDG +I ++KFL YFWWC+++LKE +TEDVPMPT      
Sbjct: 85   GFRTGLFTSPHLIDVRERFRLDGKDICEEKFLAYFWWCYDRLKEKSTEDVPMPTYFRFLA 144

Query: 501  XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIVCGISSLGFDHMEILGNTLGEIAGE 680
                     EQVDVAILEVGLGG FDATNVVQ+PIVCG++SLG+DHMEILGNTL +IAGE
Sbjct: 145  LLAFKIFSAEQVDVAILEVGLGGKFDATNVVQKPIVCGVASLGYDHMEILGNTLAQIAGE 204

Query: 681  KAGIFKQGVPAFTVPQPEEAMSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYL 860
            KAGIFK G+PAFTVPQP+EAM+VLE+KAS+LNV LQVA PLD  LLNGL+L L GEHQYL
Sbjct: 205  KAGIFKDGIPAFTVPQPDEAMNVLEEKASELNVHLQVAEPLDAKLLNGLKLRLEGEHQYL 264

Query: 861  NASLAIALCHTWLQKTGHLEGMYLDQTSSLPEQFVRGLANTSFEGRAQIVTDLYMDRP-- 1034
            NA LAIAL  TWLQ+TGH E  +++Q SSLPEQF++GL   S +GRAQIVTD Y++    
Sbjct: 265  NAGLAIALSFTWLQRTGHHEFTHMEQASSLPEQFIKGLTTASLQGRAQIVTDQYINAESY 324

Query: 1035 GDLVFYLDGAHSPESMDMCGRWFSVATREDVQQ-------HNGSKERLQA-----DLR-- 1172
            GDLVFYLDGAHSPESM+MC RWFS+A +ED Q+        N S+  ++      D R  
Sbjct: 325  GDLVFYLDGAHSPESMEMCARWFSLAIKEDSQKRTFNYPPQNNSESTIELVQEHHDERYG 384

Query: 1173 KDSTKVLLFNCMPVRDPQLLLPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDP 1352
            K S ++LLFNCM VRDPQLLLP L+ ACA HGV+F+KALFV N SVY+KVGSHALP  D 
Sbjct: 385  KSSMQILLFNCMSVRDPQLLLPPLMKACASHGVYFKKALFVANTSVYYKVGSHALPPTDS 444

Query: 1353 QVDLSWQLTLQRVWENLVCGDKAIQGGDEKAADPIREEAIEDASNSIRS-ETSSVFSSLP 1529
            Q +LSWQ  LQRVWENL+  DK   GG+ K A  + EE  EDA  S R+ E S+VF SLP
Sbjct: 445  QFNLSWQFALQRVWENLIQSDK---GGEVKHAVAVCEEGKEDAKMSGRTCENSAVFPSLP 501

Query: 1530 SAIKWLRDSAQQNRSVRFQVLVTGSLHLVGDVLRLIKK 1643
             AIKWL++S Q+NRSV +QVLVTGSLHLVGDVLRL+KK
Sbjct: 502  LAIKWLKESVQRNRSVHYQVLVTGSLHLVGDVLRLVKK 539


>ref|XP_002316257.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222865297|gb|EEF02428.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 587

 Score =  671 bits (1730), Expect = 0.0
 Identities = 355/557 (63%), Positives = 408/557 (73%), Gaps = 57/557 (10%)
 Frame = +3

Query: 141  ITKRSRADKSNKGDRFDLLYDYIKVLELDEAIPKMKIIHVAGTKGKGSTCTFTESILRNC 320
            ITKRSRADKSNKGDRFDLL+DY+K+LEL+EAI +MKIIHVAGTKGKGSTCTFTESILRNC
Sbjct: 28   ITKRSRADKSNKGDRFDLLFDYLKILELEEAISEMKIIHVAGTKGKGSTCTFTESILRNC 87

Query: 321  GFRTGLFTSPHLIDIRERFRLDGLEISQDKFLVYFWWCFNKLKETTTEDVPMPTXXXXXX 500
            GF TGLFTSPHLID+RERFRLDG++I ++KFL YFWWCF++LKE  TED+PMPT      
Sbjct: 88   GFHTGLFTSPHLIDVRERFRLDGMDICEEKFLAYFWWCFDRLKEKATEDIPMPTYFRFLA 147

Query: 501  XXXXXXXXXEQVDVAILEVGLGGTFDATNVVQEPIVCGISSLGFDHMEILGNTLGEIAGE 680
                     EQVDVAILEVGLGG FDATNVVQ+PIVCGISSLG+DHMEILGNTL +IAGE
Sbjct: 148  LLAFKIFAAEQVDVAILEVGLGGKFDATNVVQKPIVCGISSLGYDHMEILGNTLAQIAGE 207

Query: 681  KAGIFK----------------------------------------QGVPAFTVPQPEEA 740
            KAGIFK                                         G+PAFTVPQP+EA
Sbjct: 208  KAGIFKVFFRTISIRLLAMHLFLNLNTICVLIIDFLRIQVFPFPIQDGIPAFTVPQPDEA 267

Query: 741  MSVLEKKASQLNVPLQVAIPLDISLLNGLQLGLHGEHQYLNASLAIALCHTWLQKTGHLE 920
            M+VL  KAS+LNV L+VA PLD  LLNGL+L L GEHQYLNA LAIAL  TWLQ+TGH E
Sbjct: 268  MNVLVDKASKLNVTLKVAEPLDAKLLNGLKLSLEGEHQYLNAGLAIALSSTWLQRTGHHE 327

Query: 921  GMYLDQTSSLPEQFVRGLANTSFEGRAQIVTDLYM--DRPGDLVFYLDGAHSPESMDMCG 1094
                +Q S LPEQF++GL   S +GRAQ V D Y+  +R GDLVFYLDGAHSPESM+MC 
Sbjct: 328  FTCPEQASPLPEQFIKGLTTASLQGRAQKVPDQYINAERYGDLVFYLDGAHSPESMEMCA 387

Query: 1095 RWFSVATREDVQQ---------HNGSKERLQADLRKD-----STKVLLFNCMPVRDPQLL 1232
            RWFS+A +ED Q+         ++GS   L     K+     S ++L+FNCM VRDPQLL
Sbjct: 388  RWFSLAVKEDSQKKAFNHPPQNNSGSTIELTQGNPKERDGKISMQILVFNCMSVRDPQLL 447

Query: 1233 LPRLINACAHHGVHFQKALFVPNQSVYHKVGSHALPLNDPQVDLSWQLTLQRVWENLVCG 1412
            LPRL+ AC +HGV+F+KALFVPN SVY+KVGSHALP  D QVDLSWQL LQRVWENL+  
Sbjct: 448  LPRLMKACGNHGVYFKKALFVPNTSVYYKVGSHALP-TDSQVDLSWQLALQRVWENLIQS 506

Query: 1413 DKAIQGGDEKAADPIREEAIEDAS-NSIRSETSSVFSSLPSAIKWLRDSAQQNRSVRFQV 1589
            DK   GG+ K AD + EE  +D        E S+VF SLP AIKWLR+S QQN+SVR+QV
Sbjct: 507  DK---GGEAKHADAVCEEGKDDTKMGGSTCENSTVFPSLPLAIKWLRESVQQNQSVRYQV 563

Query: 1590 LVTGSLHLVGDVLRLIK 1640
            LVTGSLHLVGDVLRL+K
Sbjct: 564  LVTGSLHLVGDVLRLVK 580