BLASTX nr result
ID: Coptis21_contig00025130
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00025130
(2393 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptas... 97 6e-21
gb|AAD21699.1| Contains reverse transcriptase domain (rvt) PF|00... 96 4e-17
emb|CAN59718.1| hypothetical protein VITISV_032347 [Vitis vinifera] 90 3e-15
emb|CCA66235.1| hypothetical protein [Beta vulgaris subsp. vulga... 83 3e-13
emb|CCA65997.1| hypothetical protein [Beta vulgaris subsp. vulga... 81 1e-12
>gb|ABE87589.2| RNA-directed DNA polymerase (Reverse transcriptase); Ribonuclease H;
Endonuclease/exonuclease/phosphatase [Medicago
truncatula]
Length = 1246
Score = 97.1 bits (240), Expect(3) = 6e-21
Identities = 81/355 (22%), Positives = 153/355 (43%), Gaps = 11/355 (3%)
Frame = +3
Query: 1362 ITIVHAHSLYIYRTSLWAELAILNSLSK-PWMVIGDFNACLSITEKQGGLLPTNAAMNVF 1538
+ V+A + Y+ R LWAEL L + PW+ IGDFNA L EK+ P + F
Sbjct: 102 VAAVYASTFYLKRRQLWAELTNLQGCFQGPWLFIGDFNAVLGAHEKRRRRPPPPLSCIDF 161
Query: 1539 RDIISSNLLMEAPSDGAKFTWWNKQVGRMKILGKIGRVLFNAKLLSLY-PGWCYKTG--- 1706
+ ++NLL P+ GA +TW N ++G + ++ R + N + ++ + C G
Sbjct: 162 MNWSNANLLHHLPTLGAFYTWSNGRLGSDNVALRLDRAICNEEWVNFWRSSSCSALGNSA 221
Query: 1707 -L*LCSDHSPLHGSVKIVPKPNNAPFRFFNMWACHPDFMKLVRGRMLECSSRW*LYFCA* 1883
+ SDH PL S+ + F+FF W H D ++V + W +
Sbjct: 222 LVRHQSDHHPLLMSMDFCTSQRSGNFKFFKTWTEHEDCRRIV-------AENWSKHTRGH 274
Query: 1884 TEVKDSQRQA*TME*SSVW*Y*IQA*IEEETK----KLEQIQGRFEEDGXXXXXXXXXXX 2051
+ + + W + ++ + + ++ +IQ + G
Sbjct: 275 GMTRLQAKLKHMKQVFRHWNRTVFGDVDRKVRMAVEEVNRIQQIIDSVGFSDQLYAQELE 334
Query: 2052 XXXXXXXWMKQAQSFWKQKAKANWDNDMDRSTKYFHALVNLKKSKSMITKLKNNERTMLT 2231
+ W++K + DR+T YFH + ++ +K+ I+ L++ + ++T
Sbjct: 335 AHLILTKALHYQDELWREKLRDQRFIHGDRNTAYFHRISKVRATKNTISFLQDGD-AVIT 393
Query: 2232 SQKDIGEFIVQHYQEKFEYAQHALDFEL-ISKLPEVISDEDNNMLCRIPDKDEIK 2393
I ++ ++Q F + +L + +P ++S+ DNN L R+P E+K
Sbjct: 394 DPARIEVHVLNYFQAIFSVDNSCIQNDLVVDTIPSLVSNVDNNSLLRLPLWGEVK 448
Score = 30.0 bits (66), Expect(3) = 6e-21
Identities = 19/66 (28%), Positives = 37/66 (56%)
Frame = +1
Query: 1147 PEFIGIAEPKISPSNLPVSFISSLDMCTQFFHNCNNNRVPNLWFLWRKDLNTPTLLHHSN 1326
P I +AEP I+ ++P + S+ + +++ N PNLW LW ++++ ++ S+
Sbjct: 29 PLLIFVAEPMIAFESVPPWYWDSIGV-SKYCVNGREILQPNLWALWGREVSA-IVMFISD 86
Query: 1327 QQITVE 1344
Q I +E
Sbjct: 87 QCIALE 92
Score = 22.3 bits (46), Expect(3) = 6e-21
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +3
Query: 1068 LYWNIRGISN 1097
LYW +RGI N
Sbjct: 4 LYWTVRGIDN 13
>gb|AAD21699.1| Contains reverse transcriptase domain (rvt) PF|00078 [Arabidopsis
thaliana]
Length = 1253
Score = 95.9 bits (237), Expect = 4e-17
Identities = 80/312 (25%), Positives = 135/312 (43%), Gaps = 8/312 (2%)
Frame = +3
Query: 1359 IITIVHAHSLYIYRTSLWAELAILN-SLS---KPWMVIGDFNACLSITE-KQGGLLPTNA 1523
+++IV+A + I R LW EL +L+ SLS KPW+++GDFN L E Q L N
Sbjct: 54 VVSIVYAANEAITRKELWEELLLLSVSLSGNGKPWIMLGDFNQVLCPAEHSQATSLNVNR 113
Query: 1524 AMNVFRDIISSNLLMEAPSDGAKFTWWNKQVGRMKILGKIGRVLFNAKLLSLYPGWCYKT 1703
M VFRD + L + G FTWWNK R + K+ R+L N S +P
Sbjct: 114 RMKVFRDCLFEAELCDLVFKGNTFTWWNKSATR-PVAKKLDRILVNESWCSRFPSAYAVF 172
Query: 1704 GL*LCSDHSPLHGSVKIVPKPNNAPFRFFNMWACHPDFMKLVRGRMLECSSRW*LYFCA* 1883
G SDH+ + + PFRF+N +PDF+ LV + F
Sbjct: 173 GEPDFSDHASCGVIINPLMHREKRPFRFYNFLLQNPDFISLVGELWYSINVVGSSMFKMS 232
Query: 1884 TEVKDSQRQA*TME*SSVW*Y*IQA*IEEETKKLEQIQGRFEEDGXXXXXXXXXXXXXXX 2063
++K + T + + +E+ K+ + +
Sbjct: 233 KKLKALKNPIRTFSMENF------SNLEKRVKEAHNLVLYRQNKTLSDPTIPNAALEMEA 286
Query: 2064 XXXWM---KQAQSFWKQKAKANWDNDMDRSTKYFHALVNLKKSKSMITKLKNNERTMLTS 2234
W+ K +SF+ Q+++ W + D +T YFH + + +K+ + I + ++ + +
Sbjct: 287 QRKWLILVKAEESFFCQRSRVTWMGEGDSNTSYFHRMADSRKAVNTIHIIIDDNGVKIDT 346
Query: 2235 QKDIGEFIVQHY 2270
Q I E ++++
Sbjct: 347 QLGIKEHCIEYF 358
>emb|CAN59718.1| hypothetical protein VITISV_032347 [Vitis vinifera]
Length = 1198
Score = 89.7 bits (221), Expect = 3e-15
Identities = 90/356 (25%), Positives = 148/356 (41%), Gaps = 8/356 (2%)
Frame = +3
Query: 1350 GLYIITIVHAHSLYIYRTSLWAELAILNSLSKP-WMVIGDFNACLSITEKQGGLLPTNAA 1526
G + +T V+ + ++R W EL L+ L+ P W V GDFN I+EK G T
Sbjct: 737 GFFWLTSVYGPNKAVWRKDFWMELQDLHGLTFPRWCVGGDFNVIRRISEKMGDSRLT-VN 795
Query: 1527 MNVFRDIISSNLLMEAPSDGAKFTWWNKQVGRMKILGKIGRVLFNAKLLSLYPGWCYKTG 1706
M F + I + L++ P A FTW N QV I ++ R LF+++ S + +
Sbjct: 796 MRCFDEFIRESGLLDPPLRNAAFTWSNMQVD--PICKRLDRFLFSSEWDSFFSQSIQEAL 853
Query: 1707 L*LCSDHSPLHGSVKIVP-KPNNAPFRFFNMWACHPDFMKLVRGRMLECS-SRW*LYFCA 1880
SDHSP+ ++ P K PFRF NMW HP+F + R EC W +
Sbjct: 854 PRXTSDHSPI--CLETNPXKWGPTPFRFENMWLLHPEFKEKFRDWWQECXVEXWEXH--- 908
Query: 1881 *TEVKDSQRQA*TME*SSVW*Y*IQA*IEEETKKLEQIQGRF----EEDGXXXXXXXXXX 2048
K ++ W + + E K + GR +E
Sbjct: 909 ----KFMRKLKFIKSKLXEWNIVVFGDLRERKKHILXDLGRIDRIEQEGNLNLDLVSERT 964
Query: 2049 XXXXXXXXWMKQAQSFWKQKAKANWDNDMDRSTKYFHALVNLKKSKSMITKLKNNERTML 2228
+ + + W+ K++ W + D ++K+FH + ++S+ I L + L
Sbjct: 965 LRRKELEDLLLKEEVQWRXKSRVKWIKEGDXNSKFFHRVATGRRSRKFIKSLISERGETL 1024
Query: 2229 TSQKDIGEFIVQHYQEKFEYAQ-HALDFELISKLPEVISDEDNNMLCRIPDKDEIK 2393
+ + I E IV + + + + E I P IS+E L R ++E++
Sbjct: 1025 NNXEIISEEIVNFFGNLYSKPEGDSWKIEGIDWXP--ISEESAIWLDRXFSEEEVR 1078
>emb|CCA66235.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
Length = 1380
Score = 83.2 bits (204), Expect = 3e-13
Identities = 81/280 (28%), Positives = 123/280 (43%), Gaps = 8/280 (2%)
Frame = +3
Query: 1398 RTSLWAELAILNSLSK-PWMVIGDFNACLSITEKQGGLLPTNAAMNVFRDIISSNLLMEA 1574
R +W E+ + SK P ++IGDFN L+ ++ G L + + N FR + S L E
Sbjct: 119 RAVVWGEILEFWTTSKLPCLIIGDFNETLASNDR-GSLAISQSGSNDFRQFVQSLQLTEI 177
Query: 1575 PSDGAKFTWWNKQVGRMKILGKIGRVLFNAKLLSLYPGWCYKTGL*LCSDHSPL--HGSV 1748
P+ +FTW+ G K K+ R N + L+ YP SDH PL + SV
Sbjct: 178 PTT-ERFTWFR---GNSK--SKLDRCFVNPEWLTHYPTLKLSLLNRGLSDHCPLLLNSSV 231
Query: 1749 KIV-PKPNNAPFRFFNMWACHPDFMKLVRGRMLECSSRW*LYFCA*TEVKDSQRQA*TME 1925
+ PKP F+F N W P M+LV+ + SS L T KD +
Sbjct: 232 RNWGPKP----FKFQNCWLSDPRCMRLVKDTW-QKSSPMGLVQKLKTVKKDLKD------ 280
Query: 1926 *SSVW*Y*IQA*IEEETKKLE----QIQGRFEEDGXXXXXXXXXXXXXXXXXXWMKQAQS 2093
W + IE K+LE Q+ E WMK +S
Sbjct: 281 ----WNEKVFGNIEANIKQLEHEINQLDKISNERDLDSFELEKKKKAQVDLWSWMKTKES 336
Query: 2094 FWKQKAKANWDNDMDRSTKYFHALVNLKKSKSMITKLKNN 2213
+W Q+++ W DR+TK+FH + +++K ++ IT ++ N
Sbjct: 337 YWSQQSRIKWLKQGDRNTKFFHVVASIRKHRNSITSIEVN 376
>emb|CCA65997.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
Length = 1363
Score = 80.9 bits (198), Expect = 1e-12
Identities = 51/158 (32%), Positives = 74/158 (46%), Gaps = 1/158 (0%)
Frame = +3
Query: 1359 IITIVHAHSLYIYRTSLWAELAILNS-LSKPWMVIGDFNACLSITEKQGGLLPTNAAMNV 1535
++T +HA S+ R W +L + PW+V GD N L EK GG
Sbjct: 103 LLTGMHAPSVVSERNKYWVDLTEDSPPRGTPWLVAGDMNEVLHGNEKMGGRQVGKEQGKQ 162
Query: 1536 FRDIISSNLLMEAPSDGAKFTWWNKQVGRMKILGKIGRVLFNAKLLSLYPGWCYKTGL*L 1715
+D I++N L++ G KFTW N + G I ++ R L N++ L L+P
Sbjct: 163 CKDWIAANALLDLGFQGPKFTWTNGRTGGSLIKERLDRALVNSEWLDLFPDTKVIHLPRT 222
Query: 1716 CSDHSPLHGSVKIVPKPNNAPFRFFNMWACHPDFMKLV 1829
SDH PL P+ + PFR +WA HPDF ++
Sbjct: 223 FSDHCPLLILFNENPRSESFPFRCKEVWAYHPDFTNVI 260
Score = 58.9 bits (141), Expect = 6e-06
Identities = 33/105 (31%), Positives = 57/105 (54%)
Frame = +3
Query: 2079 KQAQSFWKQKAKANWDNDMDRSTKYFHALVNLKKSKSMITKLKNNERTMLTSQKDIGEFI 2258
KQ + FW QKA + D +TKYFH L ++ K I+ LKN+ +++ +D+ + +
Sbjct: 335 KQERVFWAQKAGIDRAKLGDMNTKYFHTLAKIRTCKRKISCLKNDNHDWVSNNEDLKKMM 394
Query: 2259 VQHYQEKFEYAQHALDFELISKLPEVISDEDNNMLCRIPDKDEIK 2393
+ H+++ F + ++ + ISDE N L R ++DEIK
Sbjct: 395 MSHFEKIFTTSMYSHQRNNSFRGECRISDEWNKRLARRVEEDEIK 439