BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00024185
         (528 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI34146.3| unnamed protein product [Vitis vinifera]              314   6e-84
ref|XP_002264236.1| PREDICTED: probable E3 ubiquitin-protein lig...   314   6e-84
ref|XP_002510629.1| guanine nucleotide exchange factor P532, put...   295   4e-78
ref|XP_002331537.1| predicted protein [Populus trichocarpa] gi|2...   294   5e-78
ref|XP_003530841.1| PREDICTED: probable E3 ubiquitin-protein lig...   293   1e-77

>emb|CBI34146.3| unnamed protein product [Vitis vinifera]
          Length = 329

 Score =  314 bits (804), Expect = 6e-84
 Identities = 145/175 (82%), Positives = 160/175 (91%)
 Frame = +3

Query: 3   TKVEILPVPVAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDI 182
           +KV+ LPVPVAAVSCGGFFTM LT+EGQLWNWGANSNYELGRGDKVGGWKPQP+P+LQD+
Sbjct: 138 SKVKALPVPVAAVSCGGFFTMVLTQEGQLWNWGANSNYELGRGDKVGGWKPQPIPSLQDV 197

Query: 183 RITQIASGGYHSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACE 362
           RI QIASGGYHSLALTD+GKVLSWG+G HGQLGHS I+N K+PTV+ ALA+E V+Y+AC 
Sbjct: 198 RIIQIASGGYHSLALTDDGKVLSWGYGQHGQLGHSSIENQKIPTVIEALADEHVIYIACG 257

Query: 363 GSSSAAITDEGKLYMWGNGKDFQLGVPGLPEVQPLPVEVKFLSEDDGLGPHHVLS 527
           GSSSAAITD GKLYMWGN KD QLGVPGLPE QPLPVEVKFL EDD LGPH+VLS
Sbjct: 258 GSSSAAITDNGKLYMWGNAKDSQLGVPGLPETQPLPVEVKFLMEDDRLGPHNVLS 312



 Score = 57.0 bits (136), Expect = 2e-06
 Identities = 34/102 (33%), Positives = 55/102 (53%), Gaps = 7/102 (6%)
 Frame = +3

Query: 153 PQPVPTLQDIRITQIASGGYHSLALTDEGKVLSWGHG-GHGQLGHSPI------QNLKVP 311
           P+ V    + +I  IA+ G H+ A+T+ G++ +WG   G G+LG  P         L +P
Sbjct: 78  PRMVEGSWNAKIRHIATAGTHTAAITESGELYTWGRDQGDGRLGLGPNCGPNEGGGLSIP 137

Query: 312 TVVAALAEERVVYVACEGSSSAAITDEGKLYMWGNGKDFQLG 437
           + V AL    V  V+C G  +  +T EG+L+ WG   +++LG
Sbjct: 138 SKVKALPVP-VAAVSCGGFFTMVLTQEGQLWNWGANSNYELG 178


>ref|XP_002264236.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2-like [Vitis
           vinifera]
          Length = 489

 Score =  314 bits (804), Expect = 6e-84
 Identities = 145/175 (82%), Positives = 160/175 (91%)
 Frame = +3

Query: 3   TKVEILPVPVAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDI 182
           +KV+ LPVPVAAVSCGGFFTM LT+EGQLWNWGANSNYELGRGDKVGGWKPQP+P+LQD+
Sbjct: 243 SKVKALPVPVAAVSCGGFFTMVLTQEGQLWNWGANSNYELGRGDKVGGWKPQPIPSLQDV 302

Query: 183 RITQIASGGYHSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACE 362
           RI QIASGGYHSLALTD+GKVLSWG+G HGQLGHS I+N K+PTV+ ALA+E V+Y+AC 
Sbjct: 303 RIIQIASGGYHSLALTDDGKVLSWGYGQHGQLGHSSIENQKIPTVIEALADEHVIYIACG 362

Query: 363 GSSSAAITDEGKLYMWGNGKDFQLGVPGLPEVQPLPVEVKFLSEDDGLGPHHVLS 527
           GSSSAAITD GKLYMWGN KD QLGVPGLPE QPLPVEVKFL EDD LGPH+VLS
Sbjct: 363 GSSSAAITDNGKLYMWGNAKDSQLGVPGLPETQPLPVEVKFLMEDDRLGPHNVLS 417



 Score = 96.7 bits (239), Expect = 2e-18
 Identities = 53/135 (39%), Positives = 77/135 (57%), Gaps = 1/135 (0%)
 Frame = +3

Query: 39  VSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDIRITQIASGGYHS 218
           +SCG  F  +L  +G+LW WG      LG G +   + P   P L  IR T  A GG HS
Sbjct: 96  ISCG-LFHSSLVVDGKLWIWGKGDGGRLGFGQEYPAFLPTLNPNLDSIRST--ALGGVHS 152

Query: 219 LALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACEGSSSAAITDEGK 398
           +AL+  G+V +WG+GG G LGHS      +P +V      ++ ++A  G+ +AAIT+ G+
Sbjct: 153 VALSSLGQVFTWGYGGFGALGHSVYHRELLPRMVEGSWNAKIRHIATAGTHTAAITESGE 212

Query: 399 LYMWGNGK-DFQLGV 440
           LY WG  + D +LG+
Sbjct: 213 LYTWGRDQGDGRLGL 227



 Score = 70.5 bits (171), Expect = 1e-10
 Identities = 44/143 (30%), Positives = 73/143 (51%), Gaps = 7/143 (4%)
 Frame = +3

Query: 30  VAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDIRITQIASGG 209
           + + + GG  ++AL+  GQ++ WG      LG         P+ V    + +I  IA+ G
Sbjct: 142 IRSTALGGVHSVALSSLGQVFTWGYGGFGALGHSVYHRELLPRMVEGSWNAKIRHIATAG 201

Query: 210 YHSLALTDEGKVLSWGHG-GHGQLGHSPI------QNLKVPTVVAALAEERVVYVACEGS 368
            H+ A+T+ G++ +WG   G G+LG  P         L +P+ V AL    V  V+C G 
Sbjct: 202 THTAAITESGELYTWGRDQGDGRLGLGPNCGPNEGGGLSIPSKVKALPVP-VAAVSCGGF 260

Query: 369 SSAAITDEGKLYMWGNGKDFQLG 437
            +  +T EG+L+ WG   +++LG
Sbjct: 261 FTMVLTQEGQLWNWGANSNYELG 283


>ref|XP_002510629.1| guanine nucleotide exchange factor P532, putative [Ricinus
           communis] gi|223551330|gb|EEF52816.1| guanine nucleotide
           exchange factor P532, putative [Ricinus communis]
          Length = 450

 Score =  295 bits (754), Expect = 4e-78
 Identities = 136/175 (77%), Positives = 152/175 (86%)
 Frame = +3

Query: 3   TKVEILPVPVAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDI 182
           +KV+ L VPVAAV CGGFFT  LT EGQ+WNWGANSNYELGRG+K GGW+P+P+P L+ +
Sbjct: 259 SKVKALTVPVAAVFCGGFFTAVLTEEGQIWNWGANSNYELGRGNKTGGWQPKPIPNLEGV 318

Query: 183 RITQIASGGYHSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACE 362
           R+ QIASGGYHSLALTDEGKVLSWG GGHGQLGH  IQN K+P V+ ALA++RVVY+AC 
Sbjct: 319 RVIQIASGGYHSLALTDEGKVLSWGFGGHGQLGHPSIQNQKIPMVIDALADQRVVYIACG 378

Query: 363 GSSSAAITDEGKLYMWGNGKDFQLGVPGLPEVQPLPVEVKFLSEDDGLGPHHVLS 527
           GS+SAAITDEGKLYMWGN KD QLGVPGLPEVQP PVEV FL EDDGLGPH VLS
Sbjct: 379 GSTSAAITDEGKLYMWGNAKDSQLGVPGLPEVQPCPVEVVFLMEDDGLGPHKVLS 433



 Score = 95.5 bits (236), Expect = 4e-18
 Identities = 56/150 (37%), Positives = 84/150 (56%), Gaps = 2/150 (1%)
 Frame = +3

Query: 3   TKVEILPVPVAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDI 182
           TK E + V    +SCG  F  AL  +G +W WG      LG G +   + P   P L   
Sbjct: 103 TKQEAIEV---GISCG-LFHSALLVDGNIWIWGKGDGGRLGFGHENPVFVPTLNPNLDPA 158

Query: 183 RITQIASGGYHSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACE 362
           +   +A GG HS+ALT  G+V +WG+GG G LGH       +P +V  ++ E++ ++A  
Sbjct: 159 KC--VALGGVHSVALTSLGQVFTWGYGGFGALGHCVYHRELLPRLVQGISNEKIRHIATS 216

Query: 363 GSSSAAITDEGKLYMWGNGK-DFQLGV-PG 446
           G+ +AAIT+ G++Y WG  + D +LG+ PG
Sbjct: 217 GTHTAAITETGEVYTWGRDEGDGRLGLGPG 246


>ref|XP_002331537.1| predicted protein [Populus trichocarpa] gi|222873761|gb|EEF10892.1|
           predicted protein [Populus trichocarpa]
          Length = 320

 Score =  294 bits (753), Expect = 5e-78
 Identities = 135/174 (77%), Positives = 151/174 (86%)
 Frame = +3

Query: 6   KVEILPVPVAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDIR 185
           KV+ LPVPVA V CGGFFTM LT EGQ+WNWGANSNYELGRGDKVGGW+P+P+P+L+D R
Sbjct: 132 KVKTLPVPVATVYCGGFFTMVLTEEGQIWNWGANSNYELGRGDKVGGWRPKPIPSLEDAR 191

Query: 186 ITQIASGGYHSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACEG 365
           I QIA GGYH LALTDEGKVLSWG GGHGQLGHS IQ+ K+P V+ ALA++ V+Y+AC G
Sbjct: 192 IIQIAGGGYHCLALTDEGKVLSWGFGGHGQLGHSSIQSQKIPAVIDALADQHVIYIACGG 251

Query: 366 SSSAAITDEGKLYMWGNGKDFQLGVPGLPEVQPLPVEVKFLSEDDGLGPHHVLS 527
           SSSAAITD+GKLYMWGN KD QLG+PGLPEVQ  PVEVKFL EDDGLG H VLS
Sbjct: 252 SSSAAITDKGKLYMWGNAKDSQLGIPGLPEVQSCPVEVKFLMEDDGLGQHSVLS 305



 Score = 75.5 bits (184), Expect = 5e-12
 Identities = 42/119 (35%), Positives = 64/119 (53%), Gaps = 2/119 (1%)
 Frame = +3

Query: 96  WGANSNYELGRGDKVGGWKPQPVPTLQDIRITQIASGGYHSLALTDEGKVLSWGHGGHGQ 275
           WG      LG G +   + P   P L  +R   IA GG HS A+T  G + +WG+GG G 
Sbjct: 2   WGKGDGGRLGFGHENPAFLPTLNPFLDSVR--SIALGGVHSAAITSLGHIFTWGYGGFGA 59

Query: 276 LGHSPIQNLKVPTVVAALAEERVVYVACEGSSSAAITDEGKLYMWGNGK-DFQLGV-PG 446
           LGHS       P +V    + ++  ++  G+ +AA+T+ G++Y WG  + D +LG+ PG
Sbjct: 60  LGHSVYHRELFPRLVEGSWDGKIRCISTSGTHTAAVTESGEVYTWGREEGDGRLGLGPG 118


>ref|XP_003530841.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Glycine
           max]
          Length = 444

 Score =  293 bits (750), Expect = 1e-77
 Identities = 132/175 (75%), Positives = 152/175 (86%)
 Frame = +3

Query: 3   TKVEILPVPVAAVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDI 182
           +KV+ LP P+AAVSCGGFFTMALT +GQLWNWGANSNYELGRGDK+GGWKP+PVP+L+++
Sbjct: 253 SKVKELPYPIAAVSCGGFFTMALTVDGQLWNWGANSNYELGRGDKIGGWKPRPVPSLENV 312

Query: 183 RITQIASGGYHSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACE 362
           +I Q+ASGGYHSLALTD+GKVLSWGHGG GQLGH  IQN K+P VV ALA E ++Y++C 
Sbjct: 313 KIIQLASGGYHSLALTDDGKVLSWGHGGQGQLGHGSIQNQKIPAVVEALAHENIIYISCG 372

Query: 363 GSSSAAITDEGKLYMWGNGKDFQLGVPGLPEVQPLPVEVKFLSEDDGLGPHHVLS 527
           GSSSAA+TD GKLYMWGN  D QLG+PGLP VQ  PVEV FL EDDGLGPH VLS
Sbjct: 373 GSSSAAVTDNGKLYMWGNANDSQLGIPGLPPVQSCPVEVNFLMEDDGLGPHKVLS 427



 Score = 94.4 bits (233), Expect = 9e-18
 Identities = 56/140 (40%), Positives = 79/140 (56%), Gaps = 4/140 (2%)
 Frame = +3

Query: 39  VSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDI--RITQIASGGY 212
           +SCG  F  +L  +G LW WG      LG G +     P  VPTL      +  +A GG 
Sbjct: 106 LSCG-LFHSSLIVDGALWIWGKGDGGRLGFGHE----NPLFVPTLNPHLDNLLSVALGGL 160

Query: 213 HSLALTDEGKVLSWGHGGHGQLGHSPIQNLKVPTVVAALAEERVVYVACEGSSSAAITDE 392
           HS+ALT +G+V +WG+GG G LGHS       P +V    E  + ++A  G+ +AAIT+ 
Sbjct: 161 HSVALTSDGEVFTWGYGGFGALGHSVYHRELFPRLVKGSWEGTIKHIATSGTHTAAITES 220

Query: 393 GKLYMWGNGK-DFQLGV-PG 446
           G+LY+WG  + D +LG+ PG
Sbjct: 221 GELYIWGRDEGDGRLGLGPG 240



 Score = 67.8 bits (164), Expect = 9e-10
 Identities = 43/141 (30%), Positives = 72/141 (51%), Gaps = 7/141 (4%)
 Frame = +3

Query: 36  AVSCGGFFTMALTREGQLWNWGANSNYELGRGDKVGGWKPQPVPTLQDIRITQIASGGYH 215
           +V+ GG  ++ALT +G+++ WG      LG         P+ V    +  I  IA+ G H
Sbjct: 154 SVALGGLHSVALTSDGEVFTWGYGGFGALGHSVYHRELFPRLVKGSWEGTIKHIATSGTH 213

Query: 216 SLALTDEGKVLSWGHG-GHGQLGHSP------IQNLKVPTVVAALAEERVVYVACEGSSS 374
           + A+T+ G++  WG   G G+LG  P         L +P+ V  L    +  V+C G  +
Sbjct: 214 TAAITESGELYIWGRDEGDGRLGLGPGRGPDHAGGLSIPSKVKELPYP-IAAVSCGGFFT 272

Query: 375 AAITDEGKLYMWGNGKDFQLG 437
            A+T +G+L+ WG   +++LG
Sbjct: 273 MALTVDGQLWNWGANSNYELG 293