BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00021042
         (671 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   177   1e-42
ref|XP_002327096.1| cytochrome P450 [Populus trichocarpa] gi|222...   176   3e-42
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    173   2e-41
ref|XP_002330305.1| cytochrome P450 [Populus trichocarpa] gi|222...   172   5e-41
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           170   2e-40

>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
           gi|302142392|emb|CBI19595.3| unnamed protein product
           [Vitis vinifera]
          Length = 527

 Score =  177 bits (450), Expect = 1e-42
 Identities = 101/225 (44%), Positives = 139/225 (61%), Gaps = 2/225 (0%)
 Frame = +2

Query: 2   QRIQMQKEVRATEVDAFFNELYQXXXXXXXXXXXXXXXXYDKDAAVVGRGPVIVEMNKWF 181
           +R+++ K VR +EVD    ELY                 + K+++     P++VE+N+W 
Sbjct: 148 RRLEILKHVRTSEVDMGIRELY---------------GLWVKNSS----RPLLVELNRWL 188

Query: 182 EELTLNLVTRMVSGKEHVGTNARRGDSEAKYYKKVIDDAARFMGNLVISDIFPSLGWLDY 361
           E++TLN++ RMV+GK + G  A    SEA+  +K I    R +G  V+SD  P L WLD 
Sbjct: 189 EDMTLNVIVRMVAGKRYFGAAAASDSSEARRCQKAISQFFRLIGIFVVSDALPFLWWLD- 247

Query: 362 LLGHDGAMKKTGKELDSIFSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGL 541
           L GH+ AMK T KELDSI   W++EH+Q R+V      + E+DFID+ LS  EE +L G 
Sbjct: 248 LQGHERAMKTTAKELDSILEGWLEEHRQ-RRVSSLIKAEGEQDFIDVMLSLQEEGRLSGF 306

Query: 542 --DTETFIKSICVGMILGGSDTTSVALTWVLSLLLNNRDILEKAQ 670
             D+ET IKS C+ +ILGGSDTT+  LTW +SLLLNNR  L+KAQ
Sbjct: 307 QYDSETSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHALKKAQ 351


>ref|XP_002327096.1| cytochrome P450 [Populus trichocarpa] gi|222835411|gb|EEE73846.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 392

 Score =  176 bits (447), Expect = 3e-42
 Identities = 101/225 (44%), Positives = 133/225 (59%), Gaps = 2/225 (0%)
 Frame = +2

Query: 2   QRIQMQKEVRATEVDAFFNELYQXXXXXXXXXXXXXXXXYDKDAAVVGRGPVIVEMNKWF 181
           +R++M K VRA+EVD    +                        A     PV+VE+ +W 
Sbjct: 13  RRLEMLKHVRASEVDIGIRDNINSW-------------------ANNSSSPVVVELKQWL 53

Query: 182 EELTLNLVTRMVSGKEHVGTNARRGDSEAKYYKKVIDDAARFMGNLVISDIFPSLGWLDY 361
           E+LTLN+V RMV+GK + G+ A   D EA+  +K I+   R +G  V+SD  P LGWLD 
Sbjct: 54  EDLTLNVVVRMVAGKRYFGSAAASDDGEARRCQKAINQFFRLIGIFVVSDALPFLGWLD- 112

Query: 362 LLGHDGAMKKTGKELDSIFSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGL 541
           L GH+ AMK T KELD+I   W+ EH+Q R+V      + E+DFID+ LS  EE QL   
Sbjct: 113 LQGHERAMKNTAKELDAILEGWLDEHRQ-RRVSAGIKDEGEQDFIDVMLSLKEEGQLSNF 171

Query: 542 --DTETFIKSICVGMILGGSDTTSVALTWVLSLLLNNRDILEKAQ 670
             D  T IKS C+ +ILGGSDTT+  LTW +SLLLNNR +L+KAQ
Sbjct: 172 QYDANTSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHMLKKAQ 216


>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  173 bits (439), Expect = 2e-41
 Identities = 86/222 (38%), Positives = 143/222 (64%)
 Frame = +2

Query: 5   RIQMQKEVRATEVDAFFNELYQXXXXXXXXXXXXXXXXYDKDAAVVGRGPVIVEMNKWFE 184
           R+Q  K+VRA+EV+  F  LY                  D+     G  P++++M KWFE
Sbjct: 150 RLQAIKDVRASEVNVCFKTLY------------------DQCKNPSGSAPILIDMKKWFE 191

Query: 185 ELTLNLVTRMVSGKEHVGTNARRGDSEAKYYKKVIDDAARFMGNLVISDIFPSLGWLDYL 364
           E++ N+V R++ G+++ G+   +G+ EA +YKKV+D+  R     + SD  P LG++D  
Sbjct: 192 EVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFAPLLGFVDIF 251

Query: 365 LGHDGAMKKTGKELDSIFSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGLD 544
            G+  AMK+  K++D+I  +W++EH++K+    +S  + ++DF+D+ LS +EES+L G D
Sbjct: 252 QGNLSAMKRNAKKVDAILENWLEEHRKKK----NSVAESQQDFMDVMLSIVEESKLSGHD 307

Query: 545 TETFIKSICVGMILGGSDTTSVALTWVLSLLLNNRDILEKAQ 670
            +  IK+ C+ MI+GG+DTT+V+LTW++SLL+NNR  L+KA+
Sbjct: 308 ADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKAR 349


>ref|XP_002330305.1| cytochrome P450 [Populus trichocarpa] gi|222871340|gb|EEF08471.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 525

 Score =  172 bits (436), Expect = 5e-41
 Identities = 90/222 (40%), Positives = 136/222 (61%)
 Frame = +2

Query: 5   RIQMQKEVRATEVDAFFNELYQXXXXXXXXXXXXXXXXYDKDAAVVGRGPVIVEMNKWFE 184
           R++M K VR TE      +L+                        +G G V+VEM + F 
Sbjct: 146 RLEMLKHVRDTETKLLLKDLHDRSI---------------NTTKKMGSGQVMVEMKEKFG 190

Query: 185 ELTLNLVTRMVSGKEHVGTNARRGDSEAKYYKKVIDDAARFMGNLVISDIFPSLGWLDYL 364
            L +N++ RM++GK + GT+   GD E++ ++K + D    +G  ++SD  P LGWLD++
Sbjct: 191 NLAMNIIVRMLAGKRYFGTDTN-GDEESRRFQKALGDFFYLLGLFLVSDAVPFLGWLDFV 249

Query: 365 LGHDGAMKKTGKELDSIFSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGLD 544
            G  G MK+T  E+D +FSSWV++H++ R + GS N++ E DFI + LS +E+ ++  +D
Sbjct: 250 KGIVGKMKRTATEIDCVFSSWVEDHRRNR-LNGSINEE-ERDFIHVMLSNLEDGKISAVD 307

Query: 545 TETFIKSICVGMILGGSDTTSVALTWVLSLLLNNRDILEKAQ 670
           T+T IK  C+ +ILGG DTT V LTW LSL+LNNR++LEKAQ
Sbjct: 308 TDTAIKGTCLSLILGGHDTTFVTLTWALSLILNNREVLEKAQ 349


>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  170 bits (431), Expect = 2e-40
 Identities = 88/223 (39%), Positives = 139/223 (62%), Gaps = 1/223 (0%)
 Frame = +2

Query: 5   RIQMQKEVRATEVDAFFNELYQXXXXXXXXXXXXXXXXYDKDAAVVGRGPVIVEMNKWFE 184
           R++  K++R +EV   F ELY                  D  A      PV +++ +WF+
Sbjct: 164 RLESIKQLRTSEVSVCFKELYDLTNKKN-----------DNGA------PVPIDLKRWFD 206

Query: 185 ELTLNLVTRMVSGKEHVGTNARRG-DSEAKYYKKVIDDAARFMGNLVISDIFPSLGWLDY 361
           E++ N++ R++ GK++ G+    G D EA +YKK++D+ +R     ++SD+ P LGWLDY
Sbjct: 207 EVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHYKKIMDELSRLSSLTMLSDMVPLLGWLDY 266

Query: 362 LLGHDGAMKKTGKELDSIFSSWVKEHQQKRKVQGSSNKDPEEDFIDITLSAIEESQLHGL 541
             G   AMK+ GKEL+SI   W++EH+ K+      + D  +DF+D+ LS  +++QL+G 
Sbjct: 267 FKGDLRAMKRNGKELNSILQKWLEEHKSKK------SSDARQDFMDVMLSISKDTQLYGH 320

Query: 542 DTETFIKSICVGMILGGSDTTSVALTWVLSLLLNNRDILEKAQ 670
           D +TFIK+ C+ MI+GG+++T VALTW+LSLL+NNR  L KA+
Sbjct: 321 DQDTFIKATCLAMIMGGTNSTEVALTWILSLLMNNRCALHKAR 363