BLASTX nr result
ID: Coptis21_contig00020581
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00020581
(1871 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002280205.1| PREDICTED: cytokinin hydroxylase [Vitis vini... 781 0.0
ref|XP_002516723.1| cytochrome P450, putative [Ricinus communis]... 765 0.0
emb|CAN79415.1| hypothetical protein VITISV_009963 [Vitis vinifera] 765 0.0
dbj|BAM09000.1| cytokinin hydroxylase-like [Solanum lycopersicum] 748 0.0
dbj|BAM08999.1| cytokinin hydroxylase-like [Solanum lycopersicum] 749 0.0
>ref|XP_002280205.1| PREDICTED: cytokinin hydroxylase [Vitis vinifera]
gi|296087282|emb|CBI33656.3| unnamed protein product
[Vitis vinifera]
Length = 515
Score = 781 bits (2017), Expect(2) = 0.0
Identities = 371/485 (76%), Positives = 434/485 (89%)
Frame = +3
Query: 156 RRIKKVMQRQGVHGPKPHFLRGNLNEVASLLAMTTCNDMDSVHHDIVSRLLPHYELWSQQ 335
RRIKK+M+ QGV GPKP FL GN+ ++A+L++ +T DMDS+ HD V RLLPH+ WS+Q
Sbjct: 32 RRIKKIMENQGVRGPKPRFLVGNIMDMAALVSKSTSTDMDSISHDTVGRLLPHFVAWSKQ 91
Query: 336 YGKRFIYWVGNEPRICLTETDKIKELLSSKYTNLSGKSWLQQQNSKHFIGRGLLMANGED 515
YGKRFIYW G EPR+CLTET+ IKELL+ KY +SGKSWLQQQ SKHFIGRGLLMANG+D
Sbjct: 92 YGKRFIYWNGPEPRMCLTETELIKELLT-KYNMISGKSWLQQQGSKHFIGRGLLMANGDD 150
Query: 516 WYHQRHIVAPAFMADKLKSHVGYMVECTHQMLQSLKSKVEIGKTEVEIGEYMSQLTADII 695
WYHQRHIVAPAFM DKLK + GYM+ECT QMLQSL++ VE G+TE EIGEYM++LTADII
Sbjct: 151 WYHQRHIVAPAFMGDKLKGYAGYMMECTTQMLQSLQNAVESGQTEFEIGEYMTRLTADII 210
Query: 696 SRTEFDSSYEKGKRIFDLLTSLQHLCAQSSRRLCFPGSRSFPTKYNREIKALKVEVERLL 875
S+TEFDSSYEKGK+IF LLT LQ+LCAQ+S+ C PGSR FP+KYNREIKALK EVERLL
Sbjct: 211 SKTEFDSSYEKGKQIFHLLTELQNLCAQASKHFCLPGSRYFPSKYNREIKALKTEVERLL 270
Query: 876 MEIIQSRKECAEVGRSTSYGNDLLGMLLTETQKKRTNGFSFNLQLLMDECKTFFFTGHET 1055
MEIIQSRK+C E+GRS+SYGNDLLG+LL E QKKR +GFS NLQL+MDECKTFFF GHET
Sbjct: 271 MEIIQSRKDCVEIGRSSSYGNDLLGLLLNEMQKKRGSGFSLNLQLIMDECKTFFFAGHET 330
Query: 1056 TALLLTWTIMLLATNPSWQDKVRTELSQVCNGETPTPEQLSKLTMLNMVINESLRLYPPA 1235
TALLLTWT MLLA+NP+WQDKVR ++++VCNGETP+ + LSKLT+LNMVINES+RLYPPA
Sbjct: 331 TALLLTWTSMLLASNPTWQDKVRAQVAEVCNGETPSVDHLSKLTLLNMVINESMRLYPPA 390
Query: 1236 SLLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDVHEFKPERFAGVGKSYAPG 1415
++LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHSEELWGKD +EF P+RFA GK +APG
Sbjct: 391 TVLPRMAFEDIKLGDLHIPKGLSIWIPVLAIHHSEELWGKDANEFNPDRFA--GKMFAPG 448
Query: 1416 LNFMPFAAGPRNCVGQAFALMESKIILAMLLSRFSFTISKNYRHAPIVVLTLKPKYGVQI 1595
+F+PFAAGPRNCVGQ+FA+ME+KIILAML+SRFSFTIS+NYRHAP+++LT+KPKYGVQI
Sbjct: 449 RHFIPFAAGPRNCVGQSFAMMEAKIILAMLVSRFSFTISQNYRHAPVIILTIKPKYGVQI 508
Query: 1596 CLKPV 1610
CLKP+
Sbjct: 509 CLKPL 513
Score = 30.8 bits (68), Expect(2) = 0.0
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +2
Query: 86 LVALFCLFLRVAYETISCYYLT 151
L+ L + LR AY TISCY+LT
Sbjct: 9 LIVLGTVLLRAAYNTISCYWLT 30
>ref|XP_002516723.1| cytochrome P450, putative [Ricinus communis]
gi|223544096|gb|EEF45621.1| cytochrome P450, putative
[Ricinus communis]
Length = 515
Score = 765 bits (1975), Expect(2) = 0.0
Identities = 363/487 (74%), Positives = 431/487 (88%)
Frame = +3
Query: 156 RRIKKVMQRQGVHGPKPHFLRGNLNEVASLLAMTTCNDMDSVHHDIVSRLLPHYELWSQQ 335
RRIKK+M++QGV GP+P L GN+ +V +L++ +T D D VHHDIV RLLPH+ WS+Q
Sbjct: 31 RRIKKIMEKQGVRGPQPRPLTGNILDVTALVSQSTSRDCDYVHHDIVYRLLPHFVTWSKQ 90
Query: 336 YGKRFIYWVGNEPRICLTETDKIKELLSSKYTNLSGKSWLQQQNSKHFIGRGLLMANGED 515
YGKRFIYW G EPR+CLTET+ IKELL+ KY+ +GKSWLQQQ SKHFIGRGLLMANG++
Sbjct: 91 YGKRFIYWNGIEPRMCLTETELIKELLT-KYSTKAGKSWLQQQGSKHFIGRGLLMANGDN 149
Query: 516 WYHQRHIVAPAFMADKLKSHVGYMVECTHQMLQSLKSKVEIGKTEVEIGEYMSQLTADII 695
WYHQRHIVAPAFM D+LKS+ G+MVECT +MLQSL+ VE G+TE EIGEYM++LTADII
Sbjct: 150 WYHQRHIVAPAFMGDRLKSYSGHMVECTKKMLQSLQKAVESGQTEFEIGEYMTRLTADII 209
Query: 696 SRTEFDSSYEKGKRIFDLLTSLQHLCAQSSRRLCFPGSRSFPTKYNREIKALKVEVERLL 875
SRTEFD SYEKGK+IF LL LQ LCAQ+SR LCFPGSR FP+KYNREIK+LK+EVERLL
Sbjct: 210 SRTEFDCSYEKGKQIFHLLNVLQRLCAQASRHLCFPGSRFFPSKYNREIKSLKMEVERLL 269
Query: 876 MEIIQSRKECAEVGRSTSYGNDLLGMLLTETQKKRTNGFSFNLQLLMDECKTFFFTGHET 1055
MEIIQSRK+C E+GRS++YGNDLLGMLL E Q KR NGFS NLQL+MDECKTFFF GHET
Sbjct: 270 MEIIQSRKDCVEIGRSSTYGNDLLGMLLNEMQSKRENGFSLNLQLIMDECKTFFFAGHET 329
Query: 1056 TALLLTWTIMLLATNPSWQDKVRTELSQVCNGETPTPEQLSKLTMLNMVINESLRLYPPA 1235
TALLLTWT+MLLA+NPSWQ+KVR E++QVCNGETP+ + L KLT+LNMVINESLRLYPPA
Sbjct: 330 TALLLTWTVMLLASNPSWQEKVRAEVNQVCNGETPSVDHLPKLTLLNMVINESLRLYPPA 389
Query: 1236 SLLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDVHEFKPERFAGVGKSYAPG 1415
++LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHSEELWGKD +EF P+RFA K++APG
Sbjct: 390 TVLPRMAFEDIKLGDLHIPKGLSIWIPVLAIHHSEELWGKDANEFNPDRFA--SKTFAPG 447
Query: 1416 LNFMPFAAGPRNCVGQAFALMESKIILAMLLSRFSFTISKNYRHAPIVVLTLKPKYGVQI 1595
+F+PFAAGPRNC+GQ FA+ME+KIILAML+S+F+F IS +YRHAP++VLT+KPKYGVQ+
Sbjct: 448 RHFIPFAAGPRNCIGQTFAMMEAKIILAMLISKFNFNISDSYRHAPVIVLTIKPKYGVQV 507
Query: 1596 CLKPVSS 1616
CL+P+++
Sbjct: 508 CLRPLNN 514
Score = 35.0 bits (79), Expect(2) = 0.0
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +2
Query: 86 LVALFCLFLRVAYETISCYYLT 151
LV +F LF +VAY+TISCY LT
Sbjct: 8 LVIVFSLFFKVAYDTISCYLLT 29
>emb|CAN79415.1| hypothetical protein VITISV_009963 [Vitis vinifera]
Length = 530
Score = 765 bits (1976), Expect(2) = 0.0
Identities = 368/500 (73%), Positives = 432/500 (86%), Gaps = 15/500 (3%)
Frame = +3
Query: 156 RRIKKVMQRQGVHGPKPHFLRGNLNEVASLLAMTTCNDMDSVHHDIVSRLLPHYELWSQQ 335
RRIKK+M+ QGV GPKP FL GN+ ++A+L++ +T DMDS+ HD V RLLPH+ WS+Q
Sbjct: 32 RRIKKIMENQGVRGPKPRFLVGNIMDMAALVSKSTSTDMDSISHDTVGRLLPHFVAWSKQ 91
Query: 336 YGKRFIYWVGNEPRICLTETDKIKELLSSKYTNLSGKSWLQQQNSKHFIGRGLLMANGED 515
YGKRFIYW G EPR+CLTET+ IKELL+ KY +SGKSWLQQQ SKHFIGRGLLMANG+D
Sbjct: 92 YGKRFIYWNGPEPRMCLTETELIKELLT-KYNMISGKSWLQQQGSKHFIGRGLLMANGDD 150
Query: 516 WYHQRHIVAPAFMADKLKSHVGYMVECTHQMLQSLKSKVEIGKTEVEIGEYMSQLTADII 695
WYHQRHIVAPAFM DKLK + GYM+ECT QMLQSL++ VE G+TE EIGEYM++LTADII
Sbjct: 151 WYHQRHIVAPAFMGDKLKGYAGYMMECTTQMLQSLQNAVESGQTEFEIGEYMTRLTADII 210
Query: 696 SRTEFDSSYEKGKRIFDLLTSLQHLCAQSSRRLCFPGS---------------RSFPTKY 830
S+TEFDSSYEKGK+IF LLT LQ+LCAQ+S+ C P + FP+KY
Sbjct: 211 SKTEFDSSYEKGKQIFHLLTELQNLCAQASKHFCLPEESGRLLSFELTTDSAFKYFPSKY 270
Query: 831 NREIKALKVEVERLLMEIIQSRKECAEVGRSTSYGNDLLGMLLTETQKKRTNGFSFNLQL 1010
NREIKALK EVERLLMEIIQSRK+C E+GRS+SYGNDLLG+LL E QKKR +GFS NLQL
Sbjct: 271 NREIKALKTEVERLLMEIIQSRKDCVEIGRSSSYGNDLLGLLLNEMQKKRGSGFSLNLQL 330
Query: 1011 LMDECKTFFFTGHETTALLLTWTIMLLATNPSWQDKVRTELSQVCNGETPTPEQLSKLTM 1190
+MDECKTFFF GHETTALLLTWT MLLA+NP+WQDKVR ++++VCNGETP+ + LSKLT+
Sbjct: 331 IMDECKTFFFAGHETTALLLTWTSMLLASNPTWQDKVRAQVAEVCNGETPSVDHLSKLTL 390
Query: 1191 LNMVINESLRLYPPASLLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDVHEF 1370
LNMVINES+RLYPPA++LPRMAFEDIKLGDL IPKGLSIWIPVLAIHHSEELWGKD +EF
Sbjct: 391 LNMVINESMRLYPPATVLPRMAFEDIKLGDLHIPKGLSIWIPVLAIHHSEELWGKDANEF 450
Query: 1371 KPERFAGVGKSYAPGLNFMPFAAGPRNCVGQAFALMESKIILAMLLSRFSFTISKNYRHA 1550
P+RFA GK +APG +F+PFAAGPRNCVGQ+FA+ME+KIILAML+SRFSFTIS+NYRHA
Sbjct: 451 NPDRFA--GKMFAPGRHFIPFAAGPRNCVGQSFAMMEAKIILAMLVSRFSFTISQNYRHA 508
Query: 1551 PIVVLTLKPKYGVQICLKPV 1610
P+++LT+KPKYGVQICLKP+
Sbjct: 509 PVIILTIKPKYGVQICLKPL 528
Score = 30.8 bits (68), Expect(2) = 0.0
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +2
Query: 86 LVALFCLFLRVAYETISCYYLT 151
L+ L + LR AY TISCY+LT
Sbjct: 9 LIVLGTVLLRAAYNTISCYWLT 30
>dbj|BAM09000.1| cytokinin hydroxylase-like [Solanum lycopersicum]
Length = 516
Score = 748 bits (1931), Expect(2) = 0.0
Identities = 357/490 (72%), Positives = 429/490 (87%), Gaps = 1/490 (0%)
Frame = +3
Query: 150 LTRRIKKVMQRQGVHGPKPHFLRGNLNEVASLLAMTTCNDMDSVHHDIVSRLLPHYELWS 329
+ +RI K+M++QGV GPKP FL GN+ ++ASL++ +T NDMDS+HHDIV+RLLPHY WS
Sbjct: 30 IPKRITKIMEKQGVRGPKPRFLVGNILDMASLVSESTSNDMDSIHHDIVARLLPHYVAWS 89
Query: 330 QQYGKRFIYWVGNEPRICLTETDKIKELLSSKYTNLSGKSWLQQQNSKHFIGRGLLMANG 509
+ YGKRFI+W G EPR+CL+E D IKEL S KY ++SGKSWLQQQ SKHFIGRGLLMANG
Sbjct: 90 KIYGKRFIFWNGTEPRMCLSEADLIKELFS-KYNSVSGKSWLQQQGSKHFIGRGLLMANG 148
Query: 510 EDWYHQRHIVAPAFMADKLKSHVGYMVECTHQMLQSLKSKVEIGKTEVEIGEYMSQLTAD 689
+DWYHQRHIVAPAF+ +KLKS+ GYMVECT ML+SL + V+ G+ E E+GEYM++LTA+
Sbjct: 149 DDWYHQRHIVAPAFIGEKLKSYAGYMVECTSGMLRSLGNAVKSGQVEFEMGEYMARLTAE 208
Query: 690 IISRTEFDSSYEKGKRIFDLLTSLQHLCAQSSRRLCFPGSRSFPTKYNREIKALKVEVER 869
IISRTEFDSSY KGK+IF LLT LQ CAQ+SR LCFPGSR FP+KYNR+IK LK+EVE
Sbjct: 209 IISRTEFDSSYAKGKQIFHLLTILQQKCAQASRHLCFPGSRFFPSKYNRDIKTLKIEVET 268
Query: 870 LLMEIIQSRKECAEVGRSTSYGNDLLGMLLTETQKKR-TNGFSFNLQLLMDECKTFFFTG 1046
LLMEIIQSRK+ E+GRS+SYGNDLLGMLL E QKKR +NGFS NLQL+MDECKTFFF G
Sbjct: 269 LLMEIIQSRKDGVEIGRSSSYGNDLLGMLLNEMQKKRSSNGFSLNLQLIMDECKTFFFAG 328
Query: 1047 HETTALLLTWTIMLLATNPSWQDKVRTELSQVCNGETPTPEQLSKLTMLNMVINESLRLY 1226
HETTALLLTWT+MLLA+NPSWQDKVR E++QVC G++PT + L KLT+L+M+INESLRLY
Sbjct: 329 HETTALLLTWTVMLLASNPSWQDKVRQEINQVCKGDSPTVDHLPKLTLLSMIINESLRLY 388
Query: 1227 PPASLLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDVHEFKPERFAGVGKSY 1406
PPA++LPRMAFED KLGDL IPKGLSIWIPVLAIHHSEE+WGKD +EF+P+RFA K +
Sbjct: 389 PPATVLPRMAFEDFKLGDLNIPKGLSIWIPVLAIHHSEEIWGKDANEFRPDRFA--SKPF 446
Query: 1407 APGLNFMPFAAGPRNCVGQAFALMESKIILAMLLSRFSFTISKNYRHAPIVVLTLKPKYG 1586
+ G NF+PFAAGPRNCVGQ+FALME+KIILAML+S+F FTIS+NYRHAP++VLT+KPKYG
Sbjct: 447 SAGRNFLPFAAGPRNCVGQSFALMEAKIILAMLISKFRFTISENYRHAPVIVLTIKPKYG 506
Query: 1587 VQICLKPVSS 1616
VQ+ L P+++
Sbjct: 507 VQVKLTPLTT 516
Score = 26.6 bits (57), Expect(2) = 0.0
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 68 MMVFCGLVALFCLFLRVAYETISCYYL 148
M+V L+ + + L++ YET+ YYL
Sbjct: 3 MVVMLFLICILLMSLKIVYETLLSYYL 29
>dbj|BAM08999.1| cytokinin hydroxylase-like [Solanum lycopersicum]
Length = 516
Score = 749 bits (1933), Expect = 0.0
Identities = 361/513 (70%), Positives = 440/513 (85%), Gaps = 5/513 (0%)
Frame = +3
Query: 93 LSFVCSLELLMKPSHVTT*----LTRRIKKVMQRQGVHGPKPHFLRGNLNEVASLLAMTT 260
L +C + + +K ++ T + RRI K+M++QGV GP+P FL GN+ ++ SL++ +T
Sbjct: 7 LLLICIILMSLKIAYETLSFYYLIPRRITKIMKKQGVCGPRPRFLVGNIFDMTSLVSKST 66
Query: 261 CNDMDSVHHDIVSRLLPHYELWSQQYGKRFIYWVGNEPRICLTETDKIKELLSSKYTNLS 440
NDMDS+HHDIV+RLLPHY WS+ YGKRFI+W G EPR+CL+E D IKEL S KY+++S
Sbjct: 67 SNDMDSIHHDIVARLLPHYVAWSKIYGKRFIFWNGTEPRMCLSEADLIKELFS-KYSSVS 125
Query: 441 GKSWLQQQNSKHFIGRGLLMANGEDWYHQRHIVAPAFMADKLKSHVGYMVECTHQMLQSL 620
GKSWLQQQ SKHFIGRGLLMANG+DWYHQRHIVAPAF+ DKLKS+ GYMVECT +L+SL
Sbjct: 126 GKSWLQQQGSKHFIGRGLLMANGDDWYHQRHIVAPAFIGDKLKSYAGYMVECTSGLLRSL 185
Query: 621 KSKVEIGKTEVEIGEYMSQLTADIISRTEFDSSYEKGKRIFDLLTSLQHLCAQSSRRLCF 800
++ V+ G+ E E+GEYM++LTA+IISRTEFDSSYEKGK+IF LLT LQ CAQ+SR LCF
Sbjct: 186 ENAVKSGQMEFEMGEYMTRLTAEIISRTEFDSSYEKGKQIFHLLTILQKKCAQASRHLCF 245
Query: 801 PGSRSFPTKYNREIKALKVEVERLLMEIIQSRKECAEVGRSTSYGNDLLGMLLTETQKKR 980
PGSR FP+KYNR+IK LK+EVE LLMEIIQSRK+ E+GRS+SYGNDLLGMLL E QKKR
Sbjct: 246 PGSRFFPSKYNRDIKTLKIEVETLLMEIIQSRKDGVEIGRSSSYGNDLLGMLLNEMQKKR 305
Query: 981 -TNGFSFNLQLLMDECKTFFFTGHETTALLLTWTIMLLATNPSWQDKVRTELSQVCNGET 1157
+NGFS NLQL+MDECKTFFF GHETTALLLTWT+MLLA+NPSWQDKVR E++QVC G++
Sbjct: 306 SSNGFSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQDKVRQEINQVCKGDS 365
Query: 1158 PTPEQLSKLTMLNMVINESLRLYPPASLLPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHS 1337
PT + L KLT+L+M+INESLRLYPPA++LPRMAFED KLGDL IPKGLSIWIPVLAIHHS
Sbjct: 366 PTVDHLPKLTLLSMIINESLRLYPPATVLPRMAFEDFKLGDLNIPKGLSIWIPVLAIHHS 425
Query: 1338 EELWGKDVHEFKPERFAGVGKSYAPGLNFMPFAAGPRNCVGQAFALMESKIILAMLLSRF 1517
EE+WGKD +EF+P+RFA K + G NF+PFAAGPRNCVGQ+FALME+KIILAML+S+F
Sbjct: 426 EEIWGKDANEFRPDRFA--SKPFTAGRNFLPFAAGPRNCVGQSFALMEAKIILAMLISKF 483
Query: 1518 SFTISKNYRHAPIVVLTLKPKYGVQICLKPVSS 1616
FTIS+NYRHAP++VLT+KPKYGVQ+ L P+++
Sbjct: 484 RFTISENYRHAPVIVLTIKPKYGVQVKLTPLTT 516