BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00020163
         (1535 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264643.2| PREDICTED: cytochrome P450 716B2-like [Vitis...   458   e-126
ref|XP_002309057.1| cytochrome P450 [Populus trichocarpa] gi|222...   457   e-126
ref|XP_002513183.1| cytochrome P450, putative [Ricinus communis]...   450   e-124
ref|XP_002528002.1| cytochrome P450, putative [Ricinus communis]...   448   e-123
gb|ABC59076.1| cytochrome P450 monooxygenase CYP716A12 [Medicago...   447   e-123

>ref|XP_002264643.2| PREDICTED: cytochrome P450 716B2-like [Vitis vinifera]
          Length = 485

 Score =  458 bits (1179), Expect = e-126
 Identities = 220/478 (46%), Positives = 322/478 (67%), Gaps = 6/478 (1%)
 Frame = +1

Query: 10   ESTMEYLICLFLGVFGFSYLIFFRYFRVRNRPP----LPPGSMGWPLVGETLELQRLNRS 177
            +S ME L+  FL +   S   + R+F  +        LPPG MG+P +GE+LE  R+ R 
Sbjct: 5    QSDMELLLLSFLLLMALSLSFWIRFFVHKLEKSSGINLPPGKMGFPFIGESLEFLRMGRK 64

Query: 178  GKPDEFLNRRVRKHNKDIFKTSILGEKTVLICGAAGNKFVFTSEKSLVLTWWPDSLKKLF 357
            G P+ F+  R+ K++  IFKT +LGE T ++CGAAGNK +F++E  LV +WWP S++K+F
Sbjct: 65   GTPERFIQDRMAKYSTQIFKTCLLGEPTAVVCGAAGNKLLFSNENKLVTSWWPRSVEKIF 124

Query: 358  GTSFFTAPYDEAVRTRKLVTAFLQQEVMHQLVERFDMTCKKCIQRDWMGKSKVMAFSLIK 537
             +S  T+  +E+++TRKL+ AFL+ E + + V   D   K  +   W     V  F L K
Sbjct: 125  PSSLQTSTKEESMKTRKLLPAFLKPEALQKYVGIMDSIAKWHLDNHWDLNETVTVFPLAK 184

Query: 538  DYAFSLACEMFTSMDDSKWETKMLEEFNILLKGVFQLPIYFPGTRHYKAVKSAKVIQYEL 717
             Y F +AC +F S+DD K   K    F+IL  GV  +PI FPGT   +A+K+A  ++ EL
Sbjct: 185  QYTFMVACRLFLSIDDPKHIAKFANPFHILAAGVMSIPINFPGTPFNRAIKAADSVRKEL 244

Query: 718  IKVIEKKR--TMRHSEGRTEKDLISYLIDAQDENGKPLSDIEIADNVILLMDSGHDTSSS 891
              +I++++   +      ++ D++S+++   DENG+ L++++IAD ++ L+  GHDT+S+
Sbjct: 245  RAIIKQRKIQVLAGKSSSSKHDILSHMLTTTDENGQFLNEMDIADKILGLLIGGHDTASA 304

Query: 892  VMMMLMKYLAESPECYHKVVQEQREIAMQKRPGELLNKEDIQKMKYSWNVIREVLRLSPP 1071
            V+  ++KYLAE P+ Y++V++EQ E+A  K+ GE+L+ EDIQKMKYSWNV  EV+RL+PP
Sbjct: 305  VITFIIKYLAELPQVYNEVLKEQMEVAAGKKSGEMLDWEDIQKMKYSWNVANEVMRLAPP 364

Query: 1072 IQGTFRKAKVGFTYAGYFIPKGWMVGWVATSTHKVADYFSDPENFDPMRFDRDVLVPHTF 1251
            +QG+FR+A   FTYAG+ IPKGW + W   +THK  DYF DPE FDP RF+ +  +P+T+
Sbjct: 365  LQGSFREAITDFTYAGFSIPKGWKLYWSTNATHKNPDYFPDPEKFDPSRFEGNGPIPYTY 424

Query: 1252 VPFGGGPRMCPGKEFARVEILVFLHNLVREFRWELEFPDEIISVDPMPTSSKGLPVYL 1425
            VPFGGGPRMCPGKE+AR+EILVF+HN+VR F W    P+E + VDPMP  +KGLP+ L
Sbjct: 425  VPFGGGPRMCPGKEYARLEILVFIHNVVRRFSWYKLHPNEDVIVDPMPMPAKGLPIRL 482


>ref|XP_002309057.1| cytochrome P450 [Populus trichocarpa] gi|222855033|gb|EEE92580.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 484

 Score =  457 bits (1176), Expect = e-126
 Identities = 219/478 (45%), Positives = 318/478 (66%), Gaps = 1/478 (0%)
 Frame = +1

Query: 1    LEFESTMEYLICLFLGVFGFSYLIFFRYFRVRNRPPLPPGSMGWPLVGETLELQRLNRSG 180
            L F S + Y I   +     SYLI    + + +   LPPG+ G PL+GE+LE     + G
Sbjct: 3    LPFISLLPYGILFIISAVSLSYLINKHKYYLSSLNNLPPGNTGLPLIGESLEFLTTGQKG 62

Query: 181  KPDEFLNRRVRKHNKDIFKTSILGEKTVLICGAAGNKFVFTSEKSLVLTWWPDSLKKLFG 360
            +P++F+  R+ K +  +FKTS+  E T + CGAAGNKF+F++E  LV  WWPDS+ K+F 
Sbjct: 63   QPEKFILDRMAKFSSKVFKTSLFCEPTAVFCGAAGNKFLFSNENKLVTAWWPDSVNKIFP 122

Query: 361  TSFFTAPYDEAVRTRKLVTAFLQQEVMHQLVERFDMTCKKCIQRDWMGKSKVMAFSLIKD 540
            +S  T+  +E+ + RKL   F + E + + +   D+  ++ +  DW GK +V  F L K 
Sbjct: 123  SSQQTSSQEESKKMRKLFPLFFKPESLQRYISVMDVIAQRHLASDWEGKQEVSVFPLAKT 182

Query: 541  YAFSLACEMFTSMDDSKWETKMLEEFNILLKGVFQLPIYFPGTRHYKAVKSAKVIQYELI 720
            Y F LAC +F SM+D +   K  + FN L  G+  +PI  P T   + VK++ V+  EL+
Sbjct: 183  YTFWLACRLFLSMEDPEEVQKFAKPFNDLAAGIISIPIDLPWTPFNRGVKASNVVHKELL 242

Query: 721  KVIEKKRT-MRHSEGRTEKDLISYLIDAQDENGKPLSDIEIADNVILLMDSGHDTSSSVM 897
            K+I++++  +  ++    +D++S+++   D+NG+ +  I+IAD ++ L+  GHDT+S+ +
Sbjct: 243  KIIKQRKIDLAENKASPTQDILSHMLTTADDNGQCMKKIDIADKILGLLVGGHDTASAAI 302

Query: 898  MMLMKYLAESPECYHKVVQEQREIAMQKRPGELLNKEDIQKMKYSWNVIREVLRLSPPIQ 1077
              ++KYLAE P  Y+K+++EQREIA  K PGELLN EDIQ+M+YSWNV  EV+R++PP+Q
Sbjct: 303  TFIVKYLAELPHVYNKLLEEQREIAKTKTPGELLNWEDIQRMRYSWNVACEVMRVAPPLQ 362

Query: 1078 GTFRKAKVGFTYAGYFIPKGWMVGWVATSTHKVADYFSDPENFDPMRFDRDVLVPHTFVP 1257
            G FR+A   F YAG+ IPKGW + W A +THK  + F +PENFDP RF+ +   P+TFVP
Sbjct: 363  GAFREAMTEFNYAGFTIPKGWKLYWSANTTHKNPECFPEPENFDPSRFEGNGPAPYTFVP 422

Query: 1258 FGGGPRMCPGKEFARVEILVFLHNLVREFRWELEFPDEIISVDPMPTSSKGLPVYLYP 1431
            FGGGPRMCPGKE+AR+EILVFLHNLV++FRWE   P E I VDPMP  SKGLP+ L+P
Sbjct: 423  FGGGPRMCPGKEYARLEILVFLHNLVKKFRWEKLLPKERIIVDPMPIPSKGLPIRLHP 480


>ref|XP_002513183.1| cytochrome P450, putative [Ricinus communis]
            gi|223547681|gb|EEF49174.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 477

 Score =  450 bits (1158), Expect = e-124
 Identities = 220/473 (46%), Positives = 314/473 (66%), Gaps = 4/473 (0%)
 Frame = +1

Query: 25   YLICLFLGVFGFSYLIFFRYFRVRNRPP---LPPGSMGWPLVGETLELQRLNRSGKPDEF 195
            +LI L L    F  L+      V  RP    LPPG  G P +GE+LE     R G P++F
Sbjct: 5    FLIALTL----FIILVTLPILAVLYRPNIINLPPGKTGLPYIGESLEFLSTGRKGHPEKF 60

Query: 196  LNRRVRKHNKDIFKTSILGEKTVLICGAAGNKFVFTSEKSLVLTWWPDSLKKLFGTSFFT 375
            L+ R+ K ++ +F+TSILGE+T ++CGA GNKF+F++E  LV  WWP S+ +LF +S  +
Sbjct: 61   LSDRMEKFSRQVFRTSILGEQTAVVCGAQGNKFLFSNENKLVTAWWPKSILRLFPSSNQS 120

Query: 376  APYDEAVRTRKLVTAFLQQEVMHQLVERFDMTCKKCIQRDWMGKSKVMAFSLIKDYAFSL 555
                E +R RK++  FL+ E + + +   D   +   Q  W  K +V  + L K Y FS+
Sbjct: 121  TILAEGMRMRKMLPHFLKPEALQRYIGVMDHMAQVHFQDSWENKQEVTVYPLAKMYTFSV 180

Query: 556  ACEMFTSMDDSKWETKMLEEFNILLKGVFQLPIYFPGTRHYKAVKSAKVIQYELIKVIEK 735
            AC++F SMDD K   K    FN +  G+  +PI FPGT   + +K++K+I+ E++++I++
Sbjct: 181  ACKVFLSMDDPKEVAKFAAPFNDMASGIISIPINFPGTSFNRGLKASKIIRNEMLRMIKQ 240

Query: 736  KRT-MRHSEGRTEKDLISYLIDAQDENGKPLSDIEIADNVILLMDSGHDTSSSVMMMLMK 912
            +R  +  ++    +D++S+++ A DE G+ L ++ IAD +I L+  GHDT+S+ +  ++K
Sbjct: 241  RRKDLAENKATPMQDILSHMLVATDEEGQRLGEVGIADKIISLLIGGHDTASATITFVVK 300

Query: 913  YLAESPECYHKVVQEQREIAMQKRPGELLNKEDIQKMKYSWNVIREVLRLSPPIQGTFRK 1092
            +LAE P+ Y +V++EQ EIA  K PGELL  EDIQKMKYSWNV  EV+RL+PP+QG+FR+
Sbjct: 301  FLAELPDIYDQVLKEQLEIAKSKEPGELLTWEDIQKMKYSWNVACEVMRLAPPLQGSFRE 360

Query: 1093 AKVGFTYAGYFIPKGWMVGWVATSTHKVADYFSDPENFDPMRFDRDVLVPHTFVPFGGGP 1272
            A   F YAG+ IPKGW + W   +THK  +YFSDPE FDP RF+     P+TFVPFGGGP
Sbjct: 361  ALHDFDYAGFSIPKGWKLYWSTHTTHKNPEYFSDPEKFDPSRFEGSGPAPYTFVPFGGGP 420

Query: 1273 RMCPGKEFARVEILVFLHNLVREFRWELEFPDEIISVDPMPTSSKGLPVYLYP 1431
            RMCPGKE+AR+EILVF+HN+ + F+W    PDE I VDPMP  +KGLPV+LYP
Sbjct: 421  RMCPGKEYARLEILVFMHNIAKRFKWNKVIPDEKIVVDPMPIPAKGLPVHLYP 473


>ref|XP_002528002.1| cytochrome P450, putative [Ricinus communis]
            gi|223532628|gb|EEF34414.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 471

 Score =  448 bits (1152), Expect = e-123
 Identities = 213/466 (45%), Positives = 313/466 (67%), Gaps = 1/466 (0%)
 Frame = +1

Query: 37   LFLGVFGFSYLIFFRYFRVRNRPPLPPGSMGWPLVGETLELQRLNRSGKPDEFLNRRVRK 216
            + L     SYLIF       +R  LPPG+ GWPL+GE++E     R G P++F+  R+ K
Sbjct: 6    VLLIALSISYLIFKHKSNASSRKNLPPGNTGWPLIGESIEFLSTGRKGHPEKFIFDRMEK 65

Query: 217  HNKDIFKTSILGEKTVLICGAAGNKFVFTSEKSLVLTWWPDSLKKLFGTSFFTAPYDEAV 396
             +  +FKTS+L E   + CGAAGNKF+F++E  LV  WWP+S+ K+F +S  T+  +E+ 
Sbjct: 66   FSSKVFKTSLLLEPAAVFCGAAGNKFLFSNENKLVTAWWPNSVNKIFPSSLQTSSQEESK 125

Query: 397  RTRKLVTAFLQQEVMHQLVERFDMTCKKCIQRDWMGKSKVMAFSLIKDYAFSLACEMFTS 576
            R RKL+  FL+ E + + +   D+  ++     W  K +V  F L K Y F LAC +F S
Sbjct: 126  RMRKLLPQFLKPEALQRYISIMDVIAQRHFAFGWNNKQQVTVFPLAKMYTFWLACRLFLS 185

Query: 577  MDDSKWETKMLEEFNILLKGVFQLPIYFPGTRHYKAVKSAKVIQYELIKVIEKKRT-MRH 753
            M+D +   K  + F++L  G+  +PI FPGT   + +K++  ++ ELIK+IE+++  +  
Sbjct: 186  MEDREEVEKFAKPFDVLASGIISIPIDFPGTPFNRGIKASNEVRRELIKMIEQRKIDLAE 245

Query: 754  SEGRTEKDLISYLIDAQDENGKPLSDIEIADNVILLMDSGHDTSSSVMMMLMKYLAESPE 933
            ++    +D++S+++   DE    +++++IAD ++ L+  GHDT+S+ +  ++KYLAE P+
Sbjct: 246  NKASPTQDILSHMLTTADEY---MNEMDIADKILGLLIGGHDTASAAITFVVKYLAEMPQ 302

Query: 934  CYHKVVQEQREIAMQKRPGELLNKEDIQKMKYSWNVIREVLRLSPPIQGTFRKAKVGFTY 1113
             Y+KV++EQ EIA  K  GELLN EDIQKM+YSWNV  EV+RL+PP+QG FR+A   FTY
Sbjct: 303  VYNKVLEEQMEIAKAKAAGELLNWEDIQKMRYSWNVACEVMRLAPPLQGAFREAMTDFTY 362

Query: 1114 AGYFIPKGWMVGWVATSTHKVADYFSDPENFDPMRFDRDVLVPHTFVPFGGGPRMCPGKE 1293
            AG+ IPKGW + W A STH+  + F +PE FDP RF+     P+TFVPFGGGPRMCPGKE
Sbjct: 363  AGFTIPKGWKLYWGANSTHRNPECFPEPEKFDPSRFEGKGPAPYTFVPFGGGPRMCPGKE 422

Query: 1294 FARVEILVFLHNLVREFRWELEFPDEIISVDPMPTSSKGLPVYLYP 1431
            +AR+EILVF+HN+V++FRWE   P+E I VDP+P  +KGLP+ L+P
Sbjct: 423  YARLEILVFMHNIVKKFRWEKLLPEEKIIVDPLPIPAKGLPLRLHP 468


>gb|ABC59076.1| cytochrome P450 monooxygenase CYP716A12 [Medicago truncatula]
          Length = 479

 Score =  447 bits (1151), Expect = e-123
 Identities = 217/471 (46%), Positives = 310/471 (65%), Gaps = 2/471 (0%)
 Frame = +1

Query: 25   YLICLFLGVFGFSYLIFFRYFRVRNRPPLPPGSMGWPLVGETLELQRLNRSGKPDEFLNR 204
            YL  L L V   S  +FF +++ ++   LPPG MG+P++GE+LE       G P++F+  
Sbjct: 6    YLSLLLLFVTFISLSLFFIFYKQKSPLNLPPGKMGYPIIGESLEFLSTGWKGHPEKFIFD 65

Query: 205  RVRKHNKDIFKTSILGEKTVLICGAAGNKFVFTSEKSLVLTWWPDSLKKLFGTSFFTAPY 384
            R+RK++ ++FKTSI+GE TV+ CGAA NKF+F++E  LV  WWPDS+ K+F T+   +  
Sbjct: 66   RMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSLDSNL 125

Query: 385  -DEAVRTRKLVTAFLQQEVMHQLVERFDMTCKKCIQRDWMGKSKVMAFSLIKDYAFSLAC 561
             +E+++ RKL+  F + E + + V   D+  ++     W  K+++  + L K Y F LAC
Sbjct: 126  KEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTFLLAC 185

Query: 562  EMFTSMDDSKWETKMLEEFNILLKGVFQLPIYFPGTRHYKAVKSAKVIQYELIKVIEKKR 741
             +F S++D     K  + F ++  G+  LPI  PGT   KA+K++  I+ ELIK+I+++R
Sbjct: 186  RLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKIIKQRR 245

Query: 742  T-MRHSEGRTEKDLISYLIDAQDENGKPLSDIEIADNVILLMDSGHDTSSSVMMMLMKYL 918
              +        +D++S+++   DENGK ++++ IAD ++ L+  GHDT+S     L+KYL
Sbjct: 246  VDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTFLVKYL 305

Query: 919  AESPECYHKVVQEQREIAMQKRPGELLNKEDIQKMKYSWNVIREVLRLSPPIQGTFRKAK 1098
             E P  Y KV QEQ EIA  K  GELLN +D++KMKYSWNV  EV+RLSPP+QG FR+A 
Sbjct: 306  GELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFREAI 365

Query: 1099 VGFTYAGYFIPKGWMVGWVATSTHKVADYFSDPENFDPMRFDRDVLVPHTFVPFGGGPRM 1278
              F + G+ IPKGW + W A STHK A+ F  PE FDP RF+ +   P+TFVPFGGGPRM
Sbjct: 366  TDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFGGGPRM 425

Query: 1279 CPGKEFARVEILVFLHNLVREFRWELEFPDEIISVDPMPTSSKGLPVYLYP 1431
            CPGKE+AR+EILVF+HNLV+ F+WE   PDE I VDP P  +K LP+ LYP
Sbjct: 426  CPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYP 476