BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00018728
         (1241 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEW67998.1| gibberellin 3 oxidase 1 [Castanea mollissima]          540   e-151
dbj|BAD91162.1| gibberellin 3-beta hydroxylase [Prunus subhirtella]   537   e-150
dbj|BAA37129.1| gibberelin 3beta-hydroxylase [Lactuca sativa]         525   e-146
ref|XP_002303276.1| gibberellin 3-oxidase [Populus trichocarpa] ...   523   e-146
gb|AAR12160.1| gibberellin 3-oxidase [Populus tremula x Populus ...   523   e-146

>gb|AEW67998.1| gibberellin 3 oxidase 1 [Castanea mollissima]
          Length = 368

 Score =  540 bits (1390), Expect = e-151
 Identities = 254/339 (74%), Positives = 295/339 (87%)
 Frame = +2

Query: 2    PDSHAWPPLVDYPSENLHDAESVPVIDLMDPNAAKLVGNACETWGVFQVTNHGIPMRLLE 181
            PDS+ W  L +YPS     +ESVP+IDL DPNA  LVG+AC+TWGVFQVTNHGIP +LL+
Sbjct: 30   PDSYKWTQLDEYPSCESFSSESVPLIDLTDPNALTLVGHACKTWGVFQVTNHGIPTKLLD 89

Query: 182  DLESEGRRLFFLPAKQKLKAARSPDGISGYGIARISSFFPKLMWSEGFTIVGSPVEHARQ 361
            D+ES  R LF LP +QKLKAARSPDG+SGYG+ARISSFFPKLMWSEGFTIVGSP+EH RQ
Sbjct: 90   DIESVSRCLFSLPQQQKLKAARSPDGVSGYGLARISSFFPKLMWSEGFTIVGSPLEHFRQ 149

Query: 362  LWPQDYIKFCDVMEEYEKAMKKLANRLMWIMLGSLGITGEDLKWASPKGDIESACAALQL 541
            LWPQ+Y KFCD++EEYEK MKKLA RLMW++LGSLGI  ED+KWA PKG+ + A AALQL
Sbjct: 150  LWPQEYSKFCDIIEEYEKEMKKLAGRLMWLILGSLGICNEDIKWAGPKGEFQGASAALQL 209

Query: 542  NSYPACPDPNRAMGLAAHTDSTLLTVLYQNSTSGLQVLREGAGWVTVPPTPGALVVNVGD 721
            NSYPACPDP++AMGLAAHTDSTLLT+L+QN+TSGLQVLREG GWVTV P  GALVVN+GD
Sbjct: 210  NSYPACPDPDQAMGLAAHTDSTLLTILHQNNTSGLQVLREGTGWVTVDPVQGALVVNIGD 269

Query: 722  LLHILSNGRYVSVLHRAVVHRTRHRLSIAYLYGPPTNIQISPLSKLIDLKHPPLYRSVTW 901
            LLHILSNG Y SVLHRA+V+RTR+RLS+AYLYGPP ++QISPLSKL+   HPPLYR VTW
Sbjct: 270  LLHILSNGLYPSVLHRAMVNRTRYRLSMAYLYGPPDSVQISPLSKLLGPSHPPLYRPVTW 329

Query: 902  SEYLGTKAKHFNKALSSVRLCGPKNGFIHLNDHSRMKLG 1018
            +EYLGTKAKHFNKALS VRLC P NG + +NDH+R+++G
Sbjct: 330  NEYLGTKAKHFNKALSCVRLCVPLNGLVDVNDHNRVQVG 368


>dbj|BAD91162.1| gibberellin 3-beta hydroxylase [Prunus subhirtella]
          Length = 372

 Score =  537 bits (1383), Expect = e-150
 Identities = 259/343 (75%), Positives = 293/343 (85%), Gaps = 4/343 (1%)
 Frame = +2

Query: 2    PDSHAWPPLVD-YPSENLH---DAESVPVIDLMDPNAAKLVGNACETWGVFQVTNHGIPM 169
            PDS+AW    D YPS       + +SVPVIDL DPNA KL G+AC TWGVFQVTNHGIP 
Sbjct: 30   PDSYAWSQNDDQYPSSPGSFGAEQDSVPVIDLSDPNALKLTGHACRTWGVFQVTNHGIPS 89

Query: 170  RLLEDLESEGRRLFFLPAKQKLKAARSPDGISGYGIARISSFFPKLMWSEGFTIVGSPVE 349
            +LL+D+ES GR LF LP +QKLKAARSPDGISGYG ARISSFF KLMWSEGFTIVGSP++
Sbjct: 90   KLLDDIESAGRSLFSLPVQQKLKAARSPDGISGYGFARISSFFQKLMWSEGFTIVGSPLD 149

Query: 350  HARQLWPQDYIKFCDVMEEYEKAMKKLANRLMWIMLGSLGITGEDLKWASPKGDIESACA 529
            H RQLWPQDY KFC+++EEYEK MK+LA RLMW+MLGSLGI+ ED+KWA PKGD   A A
Sbjct: 150  HFRQLWPQDYNKFCNIIEEYEKVMKRLAGRLMWLMLGSLGISMEDVKWAGPKGDFRDASA 209

Query: 530  ALQLNSYPACPDPNRAMGLAAHTDSTLLTVLYQNSTSGLQVLREGAGWVTVPPTPGALVV 709
            ALQLNSYPACPDP+RAMGLAAHTDSTLLT+LYQN+TSGLQVLREG GWVTVPP PGALVV
Sbjct: 210  ALQLNSYPACPDPDRAMGLAAHTDSTLLTILYQNNTSGLQVLREGTGWVTVPPLPGALVV 269

Query: 710  NVGDLLHILSNGRYVSVLHRAVVHRTRHRLSIAYLYGPPTNIQISPLSKLIDLKHPPLYR 889
            NVGDL+HILSNG Y SVLHRAVV+R+RHRLSIAYLYGPP ++QISPLSKL+    PPLYR
Sbjct: 270  NVGDLIHILSNGLYPSVLHRAVVNRSRHRLSIAYLYGPPASVQISPLSKLLGPSQPPLYR 329

Query: 890  SVTWSEYLGTKAKHFNKALSSVRLCGPKNGFIHLNDHSRMKLG 1018
             +TW+EYLGTKAKHFNKALSSVRLC P N  + ++DH+ +K+G
Sbjct: 330  PITWNEYLGTKAKHFNKALSSVRLCAPLNALVDVSDHNSVKVG 372


>dbj|BAA37129.1| gibberelin 3beta-hydroxylase [Lactuca sativa]
          Length = 363

 Score =  525 bits (1351), Expect = e-146
 Identities = 250/339 (73%), Positives = 286/339 (84%)
 Frame = +2

Query: 2    PDSHAWPPLVDYPSENLHDAESVPVIDLMDPNAAKLVGNACETWGVFQVTNHGIPMRLLE 181
            P+SHAW      PS     +E VPVI+L DPNA KLVG+AC+TWGVFQVTNHG+P  LLE
Sbjct: 29   PESHAWLSQDGSPSYGSSSSEQVPVINLKDPNAMKLVGHACKTWGVFQVTNHGVPTNLLE 88

Query: 182  DLESEGRRLFFLPAKQKLKAARSPDGISGYGIARISSFFPKLMWSEGFTIVGSPVEHARQ 361
            ++E+ GR+LF LP +QKLKAAR+PDG+SGYG+ARISSFFPKLMWSEGFTI+GSP EHAR+
Sbjct: 89   EMEAAGRKLFALPIQQKLKAARAPDGVSGYGVARISSFFPKLMWSEGFTIIGSPYEHARK 148

Query: 362  LWPQDYIKFCDVMEEYEKAMKKLANRLMWIMLGSLGITGEDLKWASPKGDIESACAALQL 541
            LWP  Y +FCDV+E Y+  M  LA RLMW+MLGSLG+T ED+KW   +G    +C ALQL
Sbjct: 149  LWPNRYSRFCDVIEGYKHEMNNLAQRLMWLMLGSLGVTTEDVKWDGSQG----SCPALQL 204

Query: 542  NSYPACPDPNRAMGLAAHTDSTLLTVLYQNSTSGLQVLREGAGWVTVPPTPGALVVNVGD 721
            NSYPACPDP+RAMGLAAHTDSTLLT+LYQN+TSGLQ  REGAGWVTVPP PGALVVNVGD
Sbjct: 205  NSYPACPDPDRAMGLAAHTDSTLLTILYQNNTSGLQAHREGAGWVTVPPIPGALVVNVGD 264

Query: 722  LLHILSNGRYVSVLHRAVVHRTRHRLSIAYLYGPPTNIQISPLSKLIDLKHPPLYRSVTW 901
            LLHILSNG Y SVLHRA+V+RT+HRLS+AYLYGPP+N+QISPLSKL D  HPPLYR VTW
Sbjct: 265  LLHILSNGLYPSVLHRAMVNRTQHRLSVAYLYGPPSNVQISPLSKLTDHVHPPLYRPVTW 324

Query: 902  SEYLGTKAKHFNKALSSVRLCGPKNGFIHLNDHSRMKLG 1018
            SEYLGTKAKHFNKALSSVRLC P NGF+  NDHS + +G
Sbjct: 325  SEYLGTKAKHFNKALSSVRLCVPLNGFVDANDHSGVPVG 363


>ref|XP_002303276.1| gibberellin 3-oxidase [Populus trichocarpa]
            gi|222840708|gb|EEE78255.1| gibberellin 3-oxidase
            [Populus trichocarpa]
          Length = 373

 Score =  523 bits (1348), Expect = e-146
 Identities = 249/343 (72%), Positives = 286/343 (83%), Gaps = 4/343 (1%)
 Frame = +2

Query: 2    PDSHAWPPLVD----YPSENLHDAESVPVIDLMDPNAAKLVGNACETWGVFQVTNHGIPM 169
            PDSH W  L D    +PS      ESVPVIDL+DPN  + +GNAC+TWGV QVTNHGIP+
Sbjct: 31   PDSHKWTQLDDIEQQHPSVESFITESVPVIDLLDPNVLQNIGNACKTWGVLQVTNHGIPI 90

Query: 170  RLLEDLESEGRRLFFLPAKQKLKAARSPDGISGYGIARISSFFPKLMWSEGFTIVGSPVE 349
             LLE +E   R LF LP +QKLKAARSPDG+SGYG+ARISSFF KLMWSEGFTIVGSP+E
Sbjct: 91   SLLESVEGVSRSLFSLPVQQKLKAARSPDGVSGYGVARISSFFSKLMWSEGFTIVGSPLE 150

Query: 350  HARQLWPQDYIKFCDVMEEYEKAMKKLANRLMWIMLGSLGITGEDLKWASPKGDIESACA 529
            H RQLWPQDY KFCDV+EE+EK M+KLA RL W+MLGSLGIT +DL WA PKG+ +   A
Sbjct: 151  HFRQLWPQDYTKFCDVIEEHEKEMQKLARRLTWLMLGSLGITKKDLNWAGPKGESKEGGA 210

Query: 530  ALQLNSYPACPDPNRAMGLAAHTDSTLLTVLYQNSTSGLQVLREGAGWVTVPPTPGALVV 709
            ALQLNSYPACPDP+ AMGLAAHTDSTLLT+LYQN+TSGLQVL+EG GWVTVPP PG LVV
Sbjct: 211  ALQLNSYPACPDPDLAMGLAAHTDSTLLTILYQNNTSGLQVLKEGIGWVTVPPIPGGLVV 270

Query: 710  NVGDLLHILSNGRYVSVLHRAVVHRTRHRLSIAYLYGPPTNIQISPLSKLIDLKHPPLYR 889
            NVGDLLHILSNG Y SVLHRAVV+RT+HRLSIAYLYGPP+++QISP+ KL+   HPPLYR
Sbjct: 271  NVGDLLHILSNGLYPSVLHRAVVNRTKHRLSIAYLYGPPSSVQISPIQKLVGPNHPPLYR 330

Query: 890  SVTWSEYLGTKAKHFNKALSSVRLCGPKNGFIHLNDHSRMKLG 1018
             +TW+EYL  KAKHFNKALSSVR+C P NG + +NDH+ +K+G
Sbjct: 331  PITWNEYLVAKAKHFNKALSSVRICAPLNGLVDVNDHNSVKVG 373


>gb|AAR12160.1| gibberellin 3-oxidase [Populus tremula x Populus tremuloides]
          Length = 373

 Score =  523 bits (1348), Expect = e-146
 Identities = 249/343 (72%), Positives = 286/343 (83%), Gaps = 4/343 (1%)
 Frame = +2

Query: 2    PDSHAWPPLVD----YPSENLHDAESVPVIDLMDPNAAKLVGNACETWGVFQVTNHGIPM 169
            PDSH W  L D    +PS      ESVPVIDL+DPN  + +GNAC+TWGV QVTNHGIP+
Sbjct: 31   PDSHKWTQLDDIEQQHPSVESFITESVPVIDLLDPNVLQNIGNACKTWGVLQVTNHGIPI 90

Query: 170  RLLEDLESEGRRLFFLPAKQKLKAARSPDGISGYGIARISSFFPKLMWSEGFTIVGSPVE 349
             LLE +E   R LF LP +QKLKAARSPDG+SGYG+ARISSFF KLMWSEGFTIVGSP+E
Sbjct: 91   SLLESVEGVSRSLFSLPVQQKLKAARSPDGVSGYGVARISSFFSKLMWSEGFTIVGSPLE 150

Query: 350  HARQLWPQDYIKFCDVMEEYEKAMKKLANRLMWIMLGSLGITGEDLKWASPKGDIESACA 529
            H RQLWPQDY KFCDV+EE+EK M+KLA RL W+MLGSLGIT +DL WA PKG+ +   A
Sbjct: 151  HFRQLWPQDYTKFCDVIEEHEKEMQKLARRLTWLMLGSLGITKKDLNWAGPKGESKEGGA 210

Query: 530  ALQLNSYPACPDPNRAMGLAAHTDSTLLTVLYQNSTSGLQVLREGAGWVTVPPTPGALVV 709
            ALQLNSYPACPDP+ AMGLAAHTDSTLLT+LYQN+TSGLQVL+EG GWVTVPP PG LVV
Sbjct: 211  ALQLNSYPACPDPDLAMGLAAHTDSTLLTILYQNNTSGLQVLKEGIGWVTVPPIPGGLVV 270

Query: 710  NVGDLLHILSNGRYVSVLHRAVVHRTRHRLSIAYLYGPPTNIQISPLSKLIDLKHPPLYR 889
            NVGDLLHILSNG Y SVLHRAVV+RT+HRLSIAYLYGPP+++QISP+ KL+   HPPLYR
Sbjct: 271  NVGDLLHILSNGLYPSVLHRAVVNRTKHRLSIAYLYGPPSSVQISPIQKLVGPNHPPLYR 330

Query: 890  SVTWSEYLGTKAKHFNKALSSVRLCGPKNGFIHLNDHSRMKLG 1018
             +TW+EYL  KAKHFNKALSSVR+C P NG + +NDH+ +K+G
Sbjct: 331  PITWNEYLVAKAKHFNKALSSVRICAPLNGLVDVNDHNSVKVG 373