BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00018586
         (772 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004136686.1| PREDICTED: probable ubiquitin conjugation fa...   233   3e-59
ref|XP_003633847.1| PREDICTED: probable ubiquitin conjugation fa...   231   1e-58
ref|XP_002873722.1| U-box domain-containing protein [Arabidopsis...   214   1e-53
gb|ABG89128.1| UFD2 [synthetic construct]                             209   7e-52
ref|NP_568313.2| putative ubiquitin conjugation factor E4 [Arabi...   209   7e-52

>ref|XP_004136686.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Cucumis
           sativus] gi|449494681|ref|XP_004159617.1| PREDICTED:
           probable ubiquitin conjugation factor E4-like [Cucumis
           sativus]
          Length = 1043

 Score =  233 bits (594), Expect = 3e-59
 Identities = 122/211 (57%), Positives = 156/211 (73%), Gaps = 6/211 (2%)
 Frame = -1

Query: 616 MASQKPQKSKEEREDGILRKIFLVSLLDTMNNDTRIVYLEQTAAELMSEDRELRLSIDIM 437
           MA+ KPQ+S EE ED ILRK+FL+SL DT ++D+RIVYLEQTAAEL+SE + LR+S D+M
Sbjct: 1   MATSKPQRSPEEVEDIILRKVFLISLTDTSDSDSRIVYLEQTAAELLSEGKPLRISRDVM 60

Query: 436 ERVLIDRLSGEFTNAENPFNYLVGCYRRAYEEGKKISNIKDQTIRHELEIVVKQAKKLAV 257
           ER++IDRLS    +AE PF YL+GCYRRA++E KKI+++KD+T+R ++EI +KQAKKL +
Sbjct: 61  ERIIIDRLSAHVPSAEPPFQYLIGCYRRAHDETKKIASMKDKTLRSDMEIALKQAKKLTI 120

Query: 256 SYCRIHLGNPDMFTTGY---VKPSVSPLLPMICSEF--ASFDGY-XXXXXXXGQTCPPGX 95
           SYCRIHLGNP++F++G       + SPLLP+I SE   +S DG+           CPPG 
Sbjct: 121 SYCRIHLGNPELFSSGADLGTNSNTSPLLPLIFSEVGGSSMDGFGASTSVGGAYQCPPGF 180

Query: 94  XXXXXXXXXXDTLEPIFKGLYEDLRGSVLKV 2
                     DTLEPI KGLYEDLRGSVLKV
Sbjct: 181 LEEFLRDSDFDTLEPILKGLYEDLRGSVLKV 211


>ref|XP_003633847.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Vitis
           vinifera] gi|296082973|emb|CBI22274.3| unnamed protein
           product [Vitis vinifera]
          Length = 1037

 Score =  231 bits (589), Expect = 1e-58
 Identities = 129/209 (61%), Positives = 155/209 (74%), Gaps = 4/209 (1%)
 Frame = -1

Query: 616 MASQKPQKSKEEREDGILRKIFLVSLLDTMNNDTRIVYLEQTAAELMSEDRELRLSIDIM 437
           MA++KPQ S +E ED IL KIFLVSL D+M +D+RIVYLE TAAE++SE R L+LS D+M
Sbjct: 1   MATKKPQLSPDEIEDIILGKIFLVSLNDSMESDSRIVYLEMTAAEILSEGRPLKLSRDLM 60

Query: 436 ERVLIDRLSGEFTNAENPFNYLVGCYRRAYEEGKKISNIKDQTIRHELEIVVKQAKKLAV 257
           ERVLIDRLSG F  AE PF YL+GCYRRA +EGKKI++ KD+ +R ELE+VVKQAKKLAV
Sbjct: 61  ERVLIDRLSGHFPGAEPPFPYLIGCYRRACDEGKKIASKKDKNLRSELELVVKQAKKLAV 120

Query: 256 SYCRIHLGNPDMFT---TGYVKPSVSPLLPMICSEF-ASFDGYXXXXXXXGQTCPPGXXX 89
           SYCRIHLGNPDMF+   +G    +VSPLLP+I SE  +S DG+          CPPG   
Sbjct: 121 SYCRIHLGNPDMFSNWDSGANDSAVSPLLPLIFSEVSSSVDGFGGSSIG----CPPGFLE 176

Query: 88  XXXXXXXXDTLEPIFKGLYEDLRGSVLKV 2
                   D+L+PIFKGLYE+LR  VLKV
Sbjct: 177 EFFRDSDFDSLDPIFKGLYENLRSIVLKV 205


>ref|XP_002873722.1| U-box domain-containing protein [Arabidopsis lyrata subsp. lyrata]
           gi|297319559|gb|EFH49981.1| U-box domain-containing
           protein [Arabidopsis lyrata subsp. lyrata]
          Length = 1038

 Score =  214 bits (546), Expect = 1e-53
 Identities = 120/215 (55%), Positives = 150/215 (69%), Gaps = 10/215 (4%)
 Frame = -1

Query: 616 MASQKPQKSKEEREDGILRKIFLVSLLDTMNNDTRIVYLEQTAAELMSEDRELRLSIDIM 437
           MA+ KPQ+S  E ED ILRKIF V+L ++ ++D RIVYLE TAAE++SE +EL LS D+M
Sbjct: 1   MATSKPQRSPAEIEDIILRKIFYVTLTESADSDPRIVYLEMTAAEILSEGKELLLSRDLM 60

Query: 436 ERVLIDRLSGEFTNAENPFNYLVGCYRRAYEEGKKISNIKDQTIRHELEIVVKQAKKLAV 257
           ERVLIDRLSG+F++AE PF YL+GCYRRAY+E KKI ++KD+ +R E+EIV KQAKKLAV
Sbjct: 61  ERVLIDRLSGDFSDAEPPFPYLIGCYRRAYDESKKIQSMKDKNLRSEMEIVTKQAKKLAV 120

Query: 256 SYCRIHLGNPDMF-----TTGYV-----KPSVSPLLPMICSEFASFDGYXXXXXXXGQTC 107
           SYCRIHLGNPDMF      TG +     K +VSP+LP+I +E  S           G   
Sbjct: 121 SYCRIHLGNPDMFGNSDKPTGGLDNRLKKRNVSPVLPLIFAEVGSGSLDMFGASSSGVQA 180

Query: 106 PPGXXXXXXXXXXXDTLEPIFKGLYEDLRGSVLKV 2
           PPG           D+L+PI K LYEDLR +V+ V
Sbjct: 181 PPGFLDEFFKDSDFDSLDPILKELYEDLRSTVINV 215


>gb|ABG89128.1| UFD2 [synthetic construct]
          Length = 1037

 Score =  209 bits (531), Expect = 7e-52
 Identities = 116/215 (53%), Positives = 146/215 (67%), Gaps = 10/215 (4%)
 Frame = -1

Query: 616 MASQKPQKSKEEREDGILRKIFLVSLLDTMNNDTRIVYLEQTAAELMSEDRELRLSIDIM 437
           MA+ KPQ+S  E ED ILRKIF V+L ++ ++D RIVYLE TAAE++SE +EL LS D+M
Sbjct: 1   MATSKPQRSPAEIEDIILRKIFYVTLTESTDSDPRIVYLEMTAAEILSEGKELLLSRDLM 60

Query: 436 ERVLIDRLSGEFTNAENPFNYLVGCYRRAYEEGKKISNIKDQTIRHELEIVVKQAKKLAV 257
           ERVLIDRLSG+F++AE PF YL+GC+RRAY+E KKI ++KD+ +R E+EIV KQAKKLAV
Sbjct: 61  ERVLIDRLSGDFSDAEPPFPYLIGCHRRAYDESKKIQSMKDKNLRSEMEIVTKQAKKLAV 120

Query: 256 SYCRIHLGNPDMFTTG----------YVKPSVSPLLPMICSEFASFDGYXXXXXXXGQTC 107
           SYCRIHLGNPDMF               K +VSP+LP+I +E  S           G   
Sbjct: 121 SYCRIHLGNPDMFGNSDKPSGGLDNRLKKRNVSPVLPLIFAEVGSGSLDMFGASSSGVQA 180

Query: 106 PPGXXXXXXXXXXXDTLEPIFKGLYEDLRGSVLKV 2
           PPG           D+L+ I K LYEDLR +V+ V
Sbjct: 181 PPGFLDEFFKDSDFDSLDSILKELYEDLRSTVINV 215


>ref|NP_568313.2| putative ubiquitin conjugation factor E4 [Arabidopsis thaliana]
           gi|75174048|sp|Q9LF41.1|UBE4_ARATH RecName:
           Full=Probable ubiquitin conjugation factor E4; AltName:
           Full=Plant U-box protein 1; AltName: Full=U-box
           domain-containing protein 1; AltName:
           Full=Ubiquitin-fusion degradation protein 2-like;
           Short=UB fusion protein 2-like
           gi|9755795|emb|CAC01739.1| ubiquitin-fusion degradation
           protein-like [Arabidopsis thaliana]
           gi|332004773|gb|AED92156.1| putative ubiquitin
           conjugation factor E4 [Arabidopsis thaliana]
          Length = 1038

 Score =  209 bits (531), Expect = 7e-52
 Identities = 116/215 (53%), Positives = 146/215 (67%), Gaps = 10/215 (4%)
 Frame = -1

Query: 616 MASQKPQKSKEEREDGILRKIFLVSLLDTMNNDTRIVYLEQTAAELMSEDRELRLSIDIM 437
           MA+ KPQ+S  E ED ILRKIF V+L ++ ++D RIVYLE TAAE++SE +EL LS D+M
Sbjct: 1   MATSKPQRSPAEIEDIILRKIFYVTLTESTDSDPRIVYLEMTAAEILSEGKELLLSRDLM 60

Query: 436 ERVLIDRLSGEFTNAENPFNYLVGCYRRAYEEGKKISNIKDQTIRHELEIVVKQAKKLAV 257
           ERVLIDRLSG+F++AE PF YL+GC+RRAY+E KKI ++KD+ +R E+EIV KQAKKLAV
Sbjct: 61  ERVLIDRLSGDFSDAEPPFPYLIGCHRRAYDESKKIQSMKDKNLRSEMEIVTKQAKKLAV 120

Query: 256 SYCRIHLGNPDMFTTG----------YVKPSVSPLLPMICSEFASFDGYXXXXXXXGQTC 107
           SYCRIHLGNPDMF               K +VSP+LP+I +E  S           G   
Sbjct: 121 SYCRIHLGNPDMFGNSDKPSGGLDNRLKKRNVSPVLPLIFAEVGSGSLDMFGASSSGVQA 180

Query: 106 PPGXXXXXXXXXXXDTLEPIFKGLYEDLRGSVLKV 2
           PPG           D+L+ I K LYEDLR +V+ V
Sbjct: 181 PPGFLDEFFKDSDFDSLDSILKELYEDLRSTVINV 215