BLASTX nr result
ID: Coptis21_contig00018440
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00018440
(1636 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta] 619 e-175
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum] 590 e-166
ref|XP_002267599.1| PREDICTED: isoflavone 2'-hydroxylase-like [V... 520 e-145
ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [V... 520 e-145
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]... 520 e-145
>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 503
Score = 619 bits (1596), Expect = e-175
Identities = 312/495 (63%), Positives = 376/495 (75%)
Frame = -1
Query: 1636 LCSILLIKFLVPSKNKNXXXXXXXXXXXXXXXXXXXXIYKSLAKLSKKYGQVMYLKFGSR 1457
+ SIL+IKFLVP + KN ++++LAKLS KYG V+YL+FGSR
Sbjct: 11 ISSILVIKFLVPKRYKNLPPGPPSLPLIGHLHLFKKPLHRTLAKLSDKYGHVLYLQFGSR 70
Query: 1456 PVIVVSSPSAVEECLSKKNDIIFANRPRLLIGKLFGHDYTSMVWADYGPNWKNLRRISAS 1277
PV+VVSSPSA E+CL+K NDIIFANRP LL+GK+ GHDYT+++WA YGPNW+NLR+ISA
Sbjct: 71 PVLVVSSPSAAEDCLAK-NDIIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLRKISAI 129
Query: 1276 ELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELTSKFFALTYNVMMRMIAGKRYY 1097
ELLS +RLQ S +R DEVKS+++RLS QG +++ VE+ + F LT NVMM MIAGKRYY
Sbjct: 130 ELLSANRLQSYSDIRHDEVKSMVQRLSGQGNEYRTVEMKTMIFELTLNVMMTMIAGKRYY 189
Query: 1096 GEHVGDSEEAKRFQELVRETLILSDASNVGDFLPIWRWIGGDKGFKNKMLGLKAKRDKFX 917
G V + E+AKRFQE+V+ TL +S ASN+GDFLPI WIG KG + K+ LK KRDKF
Sbjct: 190 GGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIG-IKGIEKKVWNLKEKRDKFL 248
Query: 916 XXXXXXXXXXXXXLKFDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWDLFAAGTDTS 737
+EK LI VLLSLQE EPE+YTD+++ GI+W L AAGTDTS
Sbjct: 249 QELIEEHRISRSGG-MQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTS 307
Query: 736 KSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPDRLLDESDVEKLPYVHSIVMETLRMYPPG 557
TMEWAMSLLLNNP V+KKAQ EID ++E RL++ESDV KLPY+HSI+ ETLR+YP G
Sbjct: 308 AVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLRIYPAG 367
Query: 556 PLMIPHEASEECIVGGYNVPRGTMLLVNLWAIENDPNVWNEPTEFVPERFLGLEGTRVGF 377
PL++PHE+SEECIVGGY VP GTMLLVN+WAI+ DPN+W EPT+F PERF G EGTR GF
Sbjct: 368 PLLVPHESSEECIVGGYKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGF 427
Query: 376 KLMPFGVGRRKCPGEGLATRVMTLTLAALIQCFDWERVGEQMVDMSQELGVTMPKALPLE 197
KLMPFG GRR CPGEGLA RV+ L L ALIQCFDWERVGE+MVDMS+ G+T+PK PLE
Sbjct: 428 KLMPFGSGRRGCPGEGLAMRVVALALGALIQCFDWERVGEEMVDMSEGPGLTLPKVHPLE 487
Query: 196 AKCRPRKNALNFIDK 152
AKCRPR ALNFI +
Sbjct: 488 AKCRPRSTALNFISQ 502
>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
Length = 507
Score = 590 bits (1520), Expect = e-166
Identities = 289/460 (62%), Positives = 360/460 (78%), Gaps = 2/460 (0%)
Frame = -1
Query: 1522 YKSLAKLSKKYGQVMYLKFGSRPVIVVSSPSAVEECLSKKNDIIFANRPRLLIGKLFGHD 1343
Y++L +S +YG V+Y +FGSRPV+VVSSPSA EECL+K NDI FANRPRL+IGK G++
Sbjct: 51 YRTLTNISNQYGPVLYFQFGSRPVLVVSSPSAAEECLTK-NDIGFANRPRLMIGKHSGNN 109
Query: 1342 YTSMVWADYGPNWKNLRRISASELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVEL 1163
YT++ WA YG NW+NLRRISA E+ SP+R+QM S +R DEVKS+++RL G ++ VE+
Sbjct: 110 YTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLV-AGYEYHKVEM 168
Query: 1162 TSKFFALTYNVMMRMIAGKRYYGEHVGDSEEAKRFQELVRETLILSDASNVGDFLPIWRW 983
S FF LT N+MMRMIAGKRYYGEHV D E+A++F+E+V ET LS ASNVGDFLP+ RW
Sbjct: 169 KSVFFELTLNIMMRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLRW 228
Query: 982 IGGDKGFKNKMLGLKAKRDKFXXXXXXXXXXXXXXLK-FDREEKQTPLIQVLLSLQEAEP 806
IG KG + +++ LK KRD+F K F EEK+ LI VLLSLQE EP
Sbjct: 229 IGY-KGIEKRLIRLKNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEP 287
Query: 805 EHYTDDVVKGIMWDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPDRLLDE 626
E+YTD++++G++W LFAAGTDTS TMEWAMSLLLNNP ++KKAQ EIDL +EP R +DE
Sbjct: 288 EYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDE 347
Query: 625 SDVEKLPYVHSIVMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWAIENDPN 446
SD+ KLPY+H I+ ETLRMYP GPL+IPHE+SE+C +GGYN+P GTMLLVNLWAI+NDPN
Sbjct: 348 SDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPN 407
Query: 445 VWNEPTEFVPERFLGLE-GTRVGFKLMPFGVGRRKCPGEGLATRVMTLTLAALIQCFDWE 269
+W EP +F PERF G + G R GFKLMPFG GRR CPGEGLA RV+ LTL +L+QCFDW
Sbjct: 408 LWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWH 467
Query: 268 RVGEQMVDMSQELGVTMPKALPLEAKCRPRKNALNFIDKL 149
RVGE+MV+MS+ G+T+PK PLEA CRPR LNF+ ++
Sbjct: 468 RVGEEMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNFLSQV 507
>ref|XP_002267599.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
Length = 508
Score = 520 bits (1340), Expect = e-145
Identities = 253/464 (54%), Positives = 346/464 (74%), Gaps = 6/464 (1%)
Frame = -1
Query: 1522 YKSLAKLSKKYGQVMYLKFGSRPVIVVSSPSAVEECLSKKNDIIFANRPRLLIGKLFGHD 1343
+++LA LS +YG +++L+ GSR ++VSSPSA EECLSK ND++FANRP+LL GK G++
Sbjct: 50 HRTLADLSARYGPIVFLRLGSRQTLLVSSPSAAEECLSK-NDVVFANRPQLLAGKYIGYN 108
Query: 1342 YTSMVWADYGPNWKNLRRISASELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVEL 1163
YTSM WA+YG +W+NLRRISA E+LS SR+QMLS +R DEV+S++ RL + G + V++
Sbjct: 109 YTSMAWANYGDHWRNLRRISALEILSTSRIQMLSGIRSDEVRSLLLRLLENGT--ETVDM 166
Query: 1162 TSKFFALTYNVMMRMIAGKRYYGEHVGDSEEAKRFQELVRETLILSDASNVGDFLPIWRW 983
+ FF +T NVMMRMIAGKRYYG +V + EE +FQE++ +T L D +N+GD+LP+ RW
Sbjct: 167 KTTFFEVTMNVMMRMIAGKRYYGGNVVEVEETAKFQEIIEDTFRLGDTTNIGDYLPVLRW 226
Query: 982 IGGDKGFKNKMLGLKAKRDKFXXXXXXXXXXXXXXLKFDRE-----EKQTPLIQVLLSLQ 818
+G KG + + L+ KRD+F + EK+ +I+VLLSLQ
Sbjct: 227 LGV-KGKEKGLRELQRKRDRFMQGLIEEHRTRMAKESYSSSSCRAGEKKKTMIEVLLSLQ 285
Query: 817 EAEPEHYTDDVVKGIMWDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPDR 638
E E E+YTD++++G+M L AG DT+ +T+EWAMSLLLNNP VLKKAQ E+D + P+
Sbjct: 286 EKEAEYYTDEIIRGLMLALLGAGIDTTSATLEWAMSLLLNNPEVLKKAQMEMDNQLGPNH 345
Query: 637 LLDESDVEKLPYVHSIVMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWAIE 458
L++ESD+ +LPY+H I+ ET RMYP GP+ +PHE+S+EC+VGGY++PRGTMLLVN+W I+
Sbjct: 346 LIEESDLSQLPYLHCIIRETQRMYPAGPI-VPHESSKECMVGGYHIPRGTMLLVNIWGIQ 404
Query: 457 NDPNVWNEPTEFVPERF-LGLEGTRVGFKLMPFGVGRRKCPGEGLATRVMTLTLAALIQC 281
NDP VW EP +F+PERF +GLEG G +LMPFG GRR CPGEGLA R++ L L +LIQC
Sbjct: 405 NDPEVWKEPRKFLPERFEVGLEGEGHGLRLMPFGSGRRGCPGEGLAIRMVGLVLGSLIQC 464
Query: 280 FDWERVGEQMVDMSQELGVTMPKALPLEAKCRPRKNALNFIDKL 149
FDW+RVGE VDMS+ +G+T+P+A PL AKCRPR +N + ++
Sbjct: 465 FDWKRVGEGKVDMSEGIGLTLPRAQPLLAKCRPRPALINLLSQI 508
>ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
Length = 507
Score = 520 bits (1339), Expect = e-145
Identities = 255/464 (54%), Positives = 346/464 (74%), Gaps = 6/464 (1%)
Frame = -1
Query: 1522 YKSLAKLSKKYGQVMYLKFGSRPVIVVSSPSAVEECLSKKNDIIFANRPRLLIGKLFGHD 1343
+++LA LS +YG +++L+ G R ++VSSPSA EECLSK ND++FANRP+LL GK G++
Sbjct: 50 HRTLADLSARYGPIVFLRLGPRQTLLVSSPSAAEECLSK-NDVVFANRPQLLSGKYIGYN 108
Query: 1342 YTSMVWADYGPNWKNLRRISASELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVEL 1163
YTSM WA+YG +W+NLRRIS E+LS SR+QMLS +R DEV+S++ RL + G + V++
Sbjct: 109 YTSMAWANYGDHWRNLRRISTLEILSTSRIQMLSGIRSDEVRSLLLRLLENGA--ETVDM 166
Query: 1162 TSKFFALTYNVMMRMIAGKRYYGEHVGDSEEAKRFQELVRETLILSDASNVGDFLPIWRW 983
+ FF +T NVMMRMIAGKRYYG +V + EE +FQE++ +T L D +N+GD+LP+ RW
Sbjct: 167 KTAFFEMTMNVMMRMIAGKRYYGGNVVEVEETAKFQEIIEDTFRLGDTTNIGDYLPVLRW 226
Query: 982 IGGDKGFKNKMLGLKAKRDKFXXXXXXXXXXXXXXLKFDRE-----EKQTPLIQVLLSLQ 818
+G KG + + L+ KRD+F + EK+T +I+VLLSLQ
Sbjct: 227 LGV-KGKEKGLRELQRKRDRFMQGLIEEHRTRMAKESYSSSSCRVGEKKT-MIEVLLSLQ 284
Query: 817 EAEPEHYTDDVVKGIMWDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPDR 638
E E E+YTD++++G+M L AGTDT+ +T+EWAMSLLLNNP VLKKAQ E+D + P+
Sbjct: 285 EKEAEYYTDEIIRGLMLALLGAGTDTTSATLEWAMSLLLNNPEVLKKAQMEMDNQLGPNH 344
Query: 637 LLDESDVEKLPYVHSIVMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWAIE 458
L++ESD+ +LPY+H I+ ET RMYP GP+ +PHE+S+EC+VGGY++PRGTMLLVN+W I+
Sbjct: 345 LIEESDLSQLPYLHCIIRETQRMYPAGPI-VPHESSKECMVGGYHIPRGTMLLVNIWGIQ 403
Query: 457 NDPNVWNEPTEFVPERF-LGLEGTRVGFKLMPFGVGRRKCPGEGLATRVMTLTLAALIQC 281
NDP VW EP +F+PERF +GLEG G +LMPFG GRR CPGEGLA R++ L L +LIQC
Sbjct: 404 NDPKVWKEPRKFLPERFEVGLEGEGHGLRLMPFGSGRRGCPGEGLAIRMVGLVLGSLIQC 463
Query: 280 FDWERVGEQMVDMSQELGVTMPKALPLEAKCRPRKNALNFIDKL 149
FDWERVGE VDMS+ +G+T+PKA PL AKCRPR +N + ++
Sbjct: 464 FDWERVGEGKVDMSEGIGLTLPKAQPLLAKCRPRPALINVLSQI 507
>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
gi|223550831|gb|EEF52317.1| cytochrome P450, putative
[Ricinus communis]
Length = 500
Score = 520 bits (1338), Expect = e-145
Identities = 259/460 (56%), Positives = 339/460 (73%), Gaps = 2/460 (0%)
Frame = -1
Query: 1522 YKSLAKLSKKYGQVMYLKFGSRPVIVVSSPSAVEECLSKKNDIIFANRPRLLIGKLFGHD 1343
++SL+ LS +YG V+ L+FG R V+VVSSPSA EECL+K D +FANRPRLL GK G++
Sbjct: 48 HRSLSTLSNRYGPVLLLQFGYRRVLVVSSPSAAEECLTKC-DTVFANRPRLLAGKHIGYN 106
Query: 1342 YTSMVWADYGPNWKNLRRISASELLSPSRLQMLSAMRFDEVKSIIKRL--SDQGCKHQNV 1169
YTS+ WA YG W+NLR++SA E+LS RLQ+LS++R DEVK +I+RL +++ C+ + V
Sbjct: 107 YTSLAWAPYGDLWRNLRKVSALEILSSHRLQLLSSIRRDEVKLLIQRLFRNNKDCR-EKV 165
Query: 1168 ELTSKFFALTYNVMMRMIAGKRYYGEHVGDSEEAKRFQELVRETLILSDASNVGDFLPIW 989
++ S FF L NVMMRMIAGKRYYGE+V EEAKRF+E+VRET + + SN+GDFLP+
Sbjct: 166 DMKSAFFELMLNVMMRMIAGKRYYGENVEQVEEAKRFREIVRETFLAAGTSNMGDFLPLV 225
Query: 988 RWIGGDKGFKNKMLGLKAKRDKFXXXXXXXXXXXXXXLKFDREEKQTPLIQVLLSLQEAE 809
+GG + ++ L +RD F EE+ +I+VLLSLQE+E
Sbjct: 226 AVVGGQE---KRLKELGKRRDGFIQDLIDEHRKRMAACS--SEERNKTMIEVLLSLQESE 280
Query: 808 PEHYTDDVVKGIMWDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPDRLLD 629
PE+YTD+ +K +M L AAGTDTS +TMEWAMSLL+NNP LKKAQ EID + DRL++
Sbjct: 281 PEYYTDETIKSLMLVLLAAGTDTSAATMEWAMSLLVNNPEALKKAQTEIDSVIGHDRLIN 340
Query: 628 ESDVEKLPYVHSIVMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWAIENDP 449
ESD KLPY++ I+ E +RMYP GPL++PHE+SEEC +GGY VP GTMLLVNLW+I+NDP
Sbjct: 341 ESDTSKLPYLNCIINEVMRMYPAGPLLVPHESSEECFIGGYRVPAGTMLLVNLWSIQNDP 400
Query: 448 NVWNEPTEFVPERFLGLEGTRVGFKLMPFGVGRRKCPGEGLATRVMTLTLAALIQCFDWE 269
VW EP F PERF G EG R GF+LMPFG GRR CPGEGLA R++ L + L+QCFDWE
Sbjct: 401 RVWEEPRNFKPERFEGCEGVRDGFRLMPFGSGRRSCPGEGLALRMVGLGIGTLLQCFDWE 460
Query: 268 RVGEQMVDMSQELGVTMPKALPLEAKCRPRKNALNFIDKL 149
RVG++M+DM++ +G+TMPKA PL +C PR + +N + +L
Sbjct: 461 RVGKEMIDMTEGVGLTMPKAQPLVVQCSPRPSMVNLLSQL 500