BLASTX nr result
ID: Coptis21_contig00017311
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00017311
(1669 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 598 e-168
ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 584 e-164
ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 578 e-162
ref|XP_002334340.1| cytochrome P450 [Populus trichocarpa] gi|222... 569 e-160
ref|XP_002321857.1| cytochrome P450 [Populus trichocarpa] gi|222... 567 e-159
>ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 502
Score = 598 bits (1541), Expect = e-168
Identities = 294/505 (58%), Positives = 366/505 (72%), Gaps = 1/505 (0%)
Frame = +2
Query: 149 MEFMGYPETILAVVCFLFLHHLSNKS-LPTNWPFIGMLPALALNAQRVHDWSTYILSQTG 325
M +GYPE +LAV+CFL LH LSN + LP NWP +GMLP + N Q++ D T +L Q+
Sbjct: 1 MASIGYPEILLAVICFLLLHRLSNSNMLPWNWPLVGMLPWIFRNIQQIQDRCTEVLEQSS 60
Query: 326 CTFWFKGPWFANMDLLCTSDPANMRHIFSTNFSNYPKGTEFNEFFDILGDGIFNSDFESW 505
TF KGPWFANMD+L TSDPAN+ +I STNFSN+PKG EF + FD+LG+GIFNSD + W
Sbjct: 61 GTFLLKGPWFANMDMLITSDPANVHYIMSTNFSNFPKGPEFLKIFDVLGEGIFNSDSDLW 120
Query: 506 KTQRKTAHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNT 685
+ QRK A LIN+++FQ FL +T+ +KV GLIPVLEHV +Q +VDLQDLF+RFTFD T
Sbjct: 121 RKQRKLAQVLINDRQFQQFLVKTTENKVDEGLIPVLEHVSKQGLLVDLQDLFQRFTFDTT 180
Query: 686 IILLSGIDPGSLSIEFNTFPYMEALDDAEEAIFYRHIMPTSCWKLKRLLGIGTEKKLSKA 865
L++G D LSIEF + +A+DD EEAIF+RH++P S WKL+R LGIG EKKLSK
Sbjct: 181 CKLITGFDTKCLSIEFPRVLFAKAMDDVEEAIFFRHVVPESLWKLQRWLGIGEEKKLSKG 240
Query: 866 KESLDQFIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKYDKFLRDTILN 1045
E++D IA+ IS K E G DLLTSYM ED K +KFLRDTI+N
Sbjct: 241 WETIDNVIAEYISMKHEELSKGIAKLQEDEEGTDLLTSYMNEDSTMELKSNKFLRDTIVN 300
Query: 1046 FTVAGRDTVSAALTWFFWLLSKNPTVELQIIEELKRKAVSPINQSKKWKSFDMGDLRGSV 1225
F +AGRDT SAALTWFFWL+SKNP VE +I EELK + +KW F +L V
Sbjct: 301 FLLAGRDTTSAALTWFFWLVSKNPLVEAKIREELKETIP---EKDQKWHIFRTEELNKLV 357
Query: 1226 YLHGALCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMDEVWGRDCL 1405
YLHGALCETLRL+P VPF+HK P + D+LPSGHRV K KIL LY+MGRM +WG+DC
Sbjct: 358 YLHGALCETLRLYPPVPFQHKAPHQADVLPSGHRVHPKMKILFSLYAMGRMTTIWGKDCF 417
Query: 1406 EFKPERWISDGGGIKYVPSYKFMAFNSGPRTCLGKEIAFTQMKIVVATLLYNYHVEVLEG 1585
EFKPERWI++ GGIKY PSYKF AFN+GPRTCLGK +AFTQMK V A +++NY+V++++G
Sbjct: 418 EFKPERWITEQGGIKYEPSYKFFAFNAGPRTCLGKGVAFTQMKAVAAAIIHNYNVQLVQG 477
Query: 1586 QSASPTNSIILYRNHGLMTRIKRRF 1660
+P SIIL+ H LM R+ +R+
Sbjct: 478 HPVAPNVSIILHMKHELMVRVTKRW 502
>ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 507
Score = 584 bits (1505), Expect = e-164
Identities = 290/508 (57%), Positives = 369/508 (72%), Gaps = 4/508 (0%)
Frame = +2
Query: 149 MEFMGYPETILAVVCFLFLHHLSN-KSLPTNWPFIGMLPALALNAQRVHDWSTYILSQTG 325
M +GYPE +LAV+ F+ L N LP NWP +GM P++ L+ R+HD T IL ++G
Sbjct: 1 MALLGYPEILLAVIFFIILGRFWNINDLPWNWPLLGMFPSVFLHLHRIHDRVTDILEKSG 60
Query: 326 CTFWFKGPWFANMDLLCTSDPANMRHIFSTNFSNYPKGTEFNEFFDILGDGIFNSDFESW 505
CTF KGPWF NMDLL T+DPAN+ +I S++FSN+PKG+EF++ FD LGDGIFNSD ESW
Sbjct: 61 CTFLVKGPWFCNMDLLVTTDPANVHYIMSSHFSNFPKGSEFSKIFDFLGDGIFNSDAESW 120
Query: 506 KTQRKTAHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQ-NRVVDLQDLFKRFTFDN 682
+ QRK +INN + FL + + +KV+ GL+P+L+++ EQ +R +DLQD+F+R TFD
Sbjct: 121 RKQRKLGQLMINNVRLYRFLGKITQNKVEKGLLPILDYLSEQEDRAMDLQDVFQRLTFDT 180
Query: 683 TIILLSGIDPGSLSIEFNTFPYMEALDDAEEAIFYRHIMPTSCWKLKRLLGIGTEKKLSK 862
T +L++G D LSIE P+ AL+DAEEAI +RH++P + W+L+R LGIG EKK K
Sbjct: 181 TCMLVTGFDSKCLSIELPEVPFATALEDAEEAILHRHVLPETLWRLQRWLGIGKEKKHQK 240
Query: 863 AKESLDQFIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKYD-KFLRDTI 1039
A E+LD IA+ IS KR+ G+DLLTSYM ED G K D KFLRDTI
Sbjct: 241 AWETLDHTIAEYISKKRDELSKGIINLQEEEDGVDLLTSYMSEDITMGLKCDHKFLRDTI 300
Query: 1040 LNFTVAGRDTVSAALTWFFWLLSKNPTVELQIIEELKRKAVSPINQSKKWK-SFDMGDLR 1216
LNF +AGRDT S+ALTWFFWL+SKNP VE +I EEL+ + P + KK + +F L
Sbjct: 301 LNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREELE--SAIPEKEDKKGRHTFKTEALN 358
Query: 1217 GSVYLHGALCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMDEVWGR 1396
VYLH ALCETLRL+P VPF+HK PL+PD+LPSGHRVD K KIL LY+MGRM +WG
Sbjct: 359 KLVYLHAALCETLRLYPPVPFQHKAPLQPDMLPSGHRVDPKMKILFSLYAMGRMTSIWGT 418
Query: 1397 DCLEFKPERWISDGGGIKYVPSYKFMAFNSGPRTCLGKEIAFTQMKIVVATLLYNYHVEV 1576
DCLEFKPERWIS+ G IK+ PSYKF AFN+GPRTCLGKE+AFTQMK V AT+++NYHV+V
Sbjct: 419 DCLEFKPERWISERGRIKHEPSYKFFAFNAGPRTCLGKEVAFTQMKAVAATIIHNYHVQV 478
Query: 1577 LEGQSASPTNSIILYRNHGLMTRIKRRF 1660
+EG +P SIILY HGLM R+ +R+
Sbjct: 479 VEGHHVAPNVSIILYMKHGLMVRVSKRW 506
>ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 507
Score = 578 bits (1490), Expect = e-162
Identities = 279/506 (55%), Positives = 359/506 (70%), Gaps = 2/506 (0%)
Frame = +2
Query: 149 MEFMGYPETILAVVCFLFLHHL-SNKSLPTNWPFIGMLPALALNAQRVHDWSTYILSQTG 325
M + YPE ++AV+ FLF+ S K +P NWP +GM L + R+HD + IL +TG
Sbjct: 1 MALVSYPEILVAVIFFLFIGGFCSKKGVPWNWPIVGMFAGLLFHVHRIHDEAAEILEKTG 60
Query: 326 CTFWFKGPWFANMDLLCTSDPANMRHIFSTNFSNYPKGTEFNEFFDILGDGIFNSDFESW 505
CTF FKGPWF NMD+L T+DPAN+ +I S+NFSN+PKGTEF + F++LGDGIFN+D ESW
Sbjct: 61 CTFLFKGPWFCNMDMLGTTDPANVHYIMSSNFSNFPKGTEFRKMFEVLGDGIFNADGESW 120
Query: 506 KTQRKTAHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNT 685
+ QRK +INN +F FL + + +KV+ GL+P+L+H+ EQ RVVDLQD+F+R TFD T
Sbjct: 121 RNQRKLGQSMINNPRFHRFLAKVTYNKVEKGLVPLLDHLSEQGRVVDLQDVFQRLTFDTT 180
Query: 686 IILLSGIDPGSLSIEFNTFPYMEALDDAEEAIFYRHIMPTSCWKLKRLLGIGTEKKLSKA 865
+L++G D LSIEF P+ AL+D EEA+FYRH +P + W+L R LGIG EKKL K
Sbjct: 181 CMLVTGFDTKCLSIEFPEVPFATALEDVEEAVFYRHALPETLWRLLRWLGIGKEKKLRKG 240
Query: 866 KESLDQFIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKYD-KFLRDTIL 1042
++LD IA+ IS KRE G DLLTSYM ED G K D KFLRDTIL
Sbjct: 241 WKTLDHTIAEYISMKREELSKGITKLREDEDGADLLTSYMTEDNAIGLKCDGKFLRDTIL 300
Query: 1043 NFTVAGRDTVSAALTWFFWLLSKNPTVELQIIEELKRKAVSPINQSKKWKSFDMGDLRGS 1222
NF +AGRDT S+ALTWFFWL+SKNP VE +I EE++ A+ K F L
Sbjct: 301 NFMIAGRDTTSSALTWFFWLVSKNPLVESKIREEIE-TAIPEKEDRKGRHIFKTEGLNKL 359
Query: 1223 VYLHGALCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMDEVWGRDC 1402
VYLH A+CE LRL+P VPF+HK P++PD+LPSGH+VD K KI +Y+MGRM +WG+DC
Sbjct: 360 VYLHAAMCEALRLYPPVPFQHKAPIQPDVLPSGHKVDPKMKIFFPVYAMGRMTAIWGKDC 419
Query: 1403 LEFKPERWISDGGGIKYVPSYKFMAFNSGPRTCLGKEIAFTQMKIVVATLLYNYHVEVLE 1582
LEFKPERWIS+ G IK+ PSYKF +FN+GPRTC+GKE+AFTQMK V AT+++NYHVE+++
Sbjct: 420 LEFKPERWISELGKIKHEPSYKFFSFNAGPRTCIGKEVAFTQMKAVAATIIHNYHVEMVK 479
Query: 1583 GQSASPTNSIILYRNHGLMTRIKRRF 1660
G +P SIILY G R+ +R+
Sbjct: 480 GHHVAPNVSIILYMKRGFKVRVSKRW 505
>ref|XP_002334340.1| cytochrome P450 [Populus trichocarpa] gi|222871210|gb|EEF08341.1|
cytochrome P450 [Populus trichocarpa]
Length = 506
Score = 569 bits (1466), Expect = e-160
Identities = 276/500 (55%), Positives = 362/500 (72%), Gaps = 3/500 (0%)
Frame = +2
Query: 170 ETILAVVCFLFLHHL--SNKSLPTNWPFIGMLPALALNAQRVHDWSTYILSQTGCTFWFK 343
E A +CFL L N LP NWP IGMLP L R HDW T +L Q+ CTF FK
Sbjct: 8 EIFFAFICFLSLIFCLRKNNGLPKNWPLIGMLPGLLFQVHRFHDWVTDVLEQSKCTFQFK 67
Query: 344 GPWFANMDLLCTSDPANMRHIFSTNFSNYPKGTEFNEFFDILGDGIFNSDFESWKTQRKT 523
GPWF +MD+L T+DPAN+ +I S+NFSN+ KG++FN+ FDILGDGI N+D + WK+QRK
Sbjct: 68 GPWFGDMDILVTADPANIHYITSSNFSNFEKGSDFNKRFDILGDGIINADRDLWKSQRKV 127
Query: 524 AHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNTIILLSG 703
A L+N++ F L +T KV+ GLIPVLEHV +Q+ V+DLQDLF+RFTFD T ++++G
Sbjct: 128 AQALVNHRLFYQRLVKTIQDKVERGLIPVLEHVYKQSLVLDLQDLFQRFTFDATCVIVTG 187
Query: 704 IDPGSLSIEFNTFPYMEALDDAEEAIFYRHIMPTSCWKLKRLLGIGTEKKLSKAKESLDQ 883
DPG LSI+ + +A+D+A EAIFYRH++P S W+L+R LGIG EKKL KA+E+LD+
Sbjct: 188 FDPGCLSIDLPEVAFSKAMDNAMEAIFYRHVLPESTWRLQRWLGIGKEKKLKKARETLDR 247
Query: 884 FIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKY-DKFLRDTILNFTVAG 1060
IA+ IS KRE GIDLLTSYM ED G K D+FLRDTI+ F +AG
Sbjct: 248 IIAEIISMKREELSKGNRLMEEDGEGIDLLTSYMSEDYNMGFKSDDEFLRDTIVTFMLAG 307
Query: 1061 RDTVSAALTWFFWLLSKNPTVELQIIEELKRKAVSPINQSKKWKSFDMGDLRGSVYLHGA 1240
RDTVS+ L+WFFWL+SKNP +I EEL K P +++K + FD+ +++ VYLHGA
Sbjct: 308 RDTVSSCLSWFFWLVSKNPAAGAKIREEL--KTTLPEKEAEKRRLFDIEEIKKLVYLHGA 365
Query: 1241 LCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMDEVWGRDCLEFKPE 1420
LCE+LRL+P + E K P+R DILPSGHRV+ KTKI+ LY+MGRM VWG DC EFKPE
Sbjct: 366 LCESLRLYPPIHSEFKSPVRQDILPSGHRVNPKTKIVFSLYAMGRMSSVWGDDCFEFKPE 425
Query: 1421 RWISDGGGIKYVPSYKFMAFNSGPRTCLGKEIAFTQMKIVVATLLYNYHVEVLEGQSASP 1600
RWI+D GG+K+ PSYKF +FN+GPRTCLGK++AFTQMK VVA ++YNY V+V++ + +P
Sbjct: 426 RWITDRGGMKHEPSYKFFSFNAGPRTCLGKDVAFTQMKAVVAAIIYNYQVQVVDEHTVTP 485
Query: 1601 TNSIILYRNHGLMTRIKRRF 1660
+ S+IL+ HG+ ++ +R+
Sbjct: 486 SQSVILHMKHGMKVKLTKRW 505
>ref|XP_002321857.1| cytochrome P450 [Populus trichocarpa] gi|222868853|gb|EEF05984.1|
cytochrome P450 [Populus trichocarpa]
Length = 506
Score = 567 bits (1461), Expect = e-159
Identities = 275/500 (55%), Positives = 361/500 (72%), Gaps = 3/500 (0%)
Frame = +2
Query: 170 ETILAVVCFLFLHHL--SNKSLPTNWPFIGMLPALALNAQRVHDWSTYILSQTGCTFWFK 343
E A +CFL L N LP NWP IGMLP L R HDW T +L Q+ CTF FK
Sbjct: 8 EIFFAFICFLSLIFCLRKNNGLPKNWPLIGMLPGLLFQVHRFHDWVTDVLEQSKCTFQFK 67
Query: 344 GPWFANMDLLCTSDPANMRHIFSTNFSNYPKGTEFNEFFDILGDGIFNSDFESWKTQRKT 523
GPWF +MD+L T+DPAN+ +I S+NFSN+ KG++FN+ FDILGDGI N+D + WK+QRK
Sbjct: 68 GPWFGDMDILVTADPANIHYITSSNFSNFEKGSDFNKRFDILGDGIINADRDLWKSQRKV 127
Query: 524 AHQLINNKKFQSFLEETSSSKVQNGLIPVLEHVLEQNRVVDLQDLFKRFTFDNTIILLSG 703
A L+N++ F L +T KV+ GLIPVLEHV +Q+ V+DLQDLF+RFTFD T ++++G
Sbjct: 128 AQALVNHRLFYQRLVKTIQDKVERGLIPVLEHVYKQSLVLDLQDLFQRFTFDATCVIVTG 187
Query: 704 IDPGSLSIEFNTFPYMEALDDAEEAIFYRHIMPTSCWKLKRLLGIGTEKKLSKAKESLDQ 883
DPG LSI+ + +A+D+A EAIFYRH++P S W+L+R LGIG EKKL KA+E+LD+
Sbjct: 188 FDPGCLSIDLPEVAFSKAMDNAMEAIFYRHVLPESTWRLQRWLGIGKEKKLKKARETLDR 247
Query: 884 FIAQCISTKREIXXXXXXXXXXXXXGIDLLTSYMEEDGVSGSKY-DKFLRDTILNFTVAG 1060
IA+ IS KRE GIDLLTSYM ED G K D+FLRDTI+ F +AG
Sbjct: 248 IIAEIISMKREELSKGNRLMEEDGEGIDLLTSYMSEDYNMGFKSDDEFLRDTIVTFMLAG 307
Query: 1061 RDTVSAALTWFFWLLSKNPTVELQIIEELKRKAVSPINQSKKWKSFDMGDLRGSVYLHGA 1240
RDTVS+ L+WFFWL+SKNP +I EEL K P +++K + FD+ +++ VYLHGA
Sbjct: 308 RDTVSSCLSWFFWLVSKNPAAGAKIREEL--KTTLPEKEAEKRRLFDIEEIKKLVYLHGA 365
Query: 1241 LCETLRLFPSVPFEHKRPLRPDILPSGHRVDTKTKILPLLYSMGRMDEVWGRDCLEFKPE 1420
LCE+LRL+P + E K P+R DILPSGHRV+ K KI+ LY+MGRM VWG DC EFKPE
Sbjct: 366 LCESLRLYPPIHSEFKSPVRQDILPSGHRVNPKAKIVFSLYAMGRMSSVWGDDCFEFKPE 425
Query: 1421 RWISDGGGIKYVPSYKFMAFNSGPRTCLGKEIAFTQMKIVVATLLYNYHVEVLEGQSASP 1600
RWI+D GG+K+ PSYKF +FN+GPRTCLGK++AFTQMK VVA ++YNY V+V++ + +P
Sbjct: 426 RWITDRGGMKHEPSYKFFSFNAGPRTCLGKDVAFTQMKAVVAAIIYNYQVQVVDEHTVTP 485
Query: 1601 TNSIILYRNHGLMTRIKRRF 1660
+ S+IL+ HG+ ++ +R+
Sbjct: 486 SQSVILHMKHGMKVKLTKRW 505