BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00016999
         (1113 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   494   e-153
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   427   e-131
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...   380   e-116
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        320   9e-97
gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum f...   355   1e-95

>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  494 bits (1273), Expect(2) = e-153
 Identities = 242/261 (92%), Positives = 245/261 (93%)
 Frame = -2

Query: 1004 MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPV 825
            MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHN+GTSMTLSELSSRLPSQPV
Sbjct: 1    MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPV 60

Query: 824  SEDALYRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMA 645
            +EDALYRVMRYLVHMKLFTK SIDGELRYGLAPPAK+LVKGWDKCMVGSILAITDKDFMA
Sbjct: 61   NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 120

Query: 644  PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 465
            PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN
Sbjct: 121  PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 180

Query: 464  IFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 285
            IFN               VRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI
Sbjct: 181  IFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 240

Query: 284  PKADAIMMKCILHDWDDKECI 222
            PKADAIMMKCILHDWDDKECI
Sbjct: 241  PKADAIMMKCILHDWDDKECI 261



 Score = 73.9 bits (180), Expect(2) = e-153
 Identities = 38/51 (74%), Positives = 38/51 (74%)
 Frame = -3

Query: 154 ECIEILKRCKEAXXXXXXXXXXXXXVLNVQSEHPYTKMRLTLDLDMMLNTG 2
           ECIEILKRCKEA             VLNVQSEHPYTKMRLTLDLDMMLNTG
Sbjct: 259 ECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLDLDMMLNTG 309


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
           glaucum]
          Length = 350

 Score =  427 bits (1097), Expect(2) = e-131
 Identities = 205/261 (78%), Positives = 227/261 (86%), Gaps = 2/261 (0%)
 Frame = -2

Query: 998 VKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVSE 819
           + K+NLSSQAKLWNFIYGFA+SLVLK AVQLDLANIIHN+G+ MTLSELS  LPSQPV++
Sbjct: 4   INKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQ 63

Query: 818 DALYRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMAPW 639
           DALYRV+RYLVHMKLFTK SIDGELRYGLAPPAKFLVKGWDKCM+G+IL ITDKDFMAPW
Sbjct: 64  DALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPW 123

Query: 638 HYLKDGL--SGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 465
           HYLK+G+   G + TAFEKALGTNIW YMAEHPEKNQLFNE MAND+RLIMSALVKEC +
Sbjct: 124 HYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSS 183

Query: 464 IFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 285
           +F+               VRNIA AFPHIKCTVYDLPHVIADSPGY+E++ + GDMFK+I
Sbjct: 184 MFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYI 243

Query: 284 PKADAIMMKCILHDWDDKECI 222
           P ADAIMMKCILHDWDDKECI
Sbjct: 244 PNADAIMMKCILHDWDDKECI 264



 Score = 69.3 bits (168), Expect(2) = e-131
 Identities = 34/51 (66%), Positives = 38/51 (74%)
 Frame = -3

Query: 154 ECIEILKRCKEAXXXXXXXXXXXXXVLNVQSEHPYTKMRLTLDLDMMLNTG 2
           ECIEILKRCK+A             +L+V+SEHPYTKMRLTLDLDMMLNTG
Sbjct: 262 ECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTLDLDMMLNTG 312


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum
           flavum]
          Length = 346

 Score =  380 bits (977), Expect(2) = e-116
 Identities = 186/258 (72%), Positives = 214/258 (82%), Gaps = 2/258 (0%)
 Frame = -2

Query: 989 DNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVSEDAL 810
           ++LSSQAKLWN+IYGFA+SLVLKCAVQLDLANIIH++GT MTLSELSSRLPSQPV++DAL
Sbjct: 2   EDLSSQAKLWNYIYGFADSLVLKCAVQLDLANIIHDHGTPMTLSELSSRLPSQPVNQDAL 61

Query: 809 YRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMAPWHYL 630
           YRVMRYLVH+KLFTK SIDGELRYGLAPPAKFLVKGWD CM+GSIL + D DFMAP+HY+
Sbjct: 62  YRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMVDTDFMAPYHYI 121

Query: 629 KDGL--SGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFN 456
           KD +   G S TAFEKA GTNIW Y+AEHPEKNQLFNE MAN+SRL MS L+K+C + F 
Sbjct: 122 KDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMSGLMKDCSSTFE 181

Query: 455 XXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPKA 276
                          V+ IA AFP+IK TVYDLPHVIAD+PG +E++ V GDMFK+IP A
Sbjct: 182 GLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEINFVEGDMFKYIPNA 241

Query: 275 DAIMMKCILHDWDDKECI 222
           DAI+MK +LHDWDD ECI
Sbjct: 242 DAILMKGVLHDWDDNECI 259



 Score = 67.0 bits (162), Expect(2) = e-116
 Identities = 34/49 (69%), Positives = 36/49 (73%)
 Frame = -3

Query: 154 ECIEILKRCKEAXXXXXXXXXXXXXVLNVQSEHPYTKMRLTLDLDMMLN 8
           ECIEILKRCK+A             VLNV+SEHPYTKMRLTLDLDMMLN
Sbjct: 257 ECIEILKRCKDAVPRDGGKVIIIDVVLNVKSEHPYTKMRLTLDLDMMLN 305


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  320 bits (819), Expect(2) = 9e-97
 Identities = 157/266 (59%), Positives = 199/266 (74%), Gaps = 3/266 (1%)
 Frame = -2

Query: 1010 IEME-VKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPS 834
            +EME   K + ++QAK+W  IYGFAESL+LK AV+L++A+ IHN+G  MTLSEL+S+LP 
Sbjct: 1    MEMEGADKIDQATQAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPV 60

Query: 833  QPVSEDALYRVMRYLVHMKLFTKESIDGE--LRYGLAPPAKFLVKGWDKCMVGSILAITD 660
            QPV  D LYRVMRYLVHMKLF K+  + E  ++Y L PPAKFL+KGW+K MV +IL I D
Sbjct: 61   QPVDLDRLYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLIND 120

Query: 659  KDFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALV 480
            KDFM+PWH+LK+GL   S  AF+KALG +IW YM+ +P+KN++FNE MA DSRL+ SALV
Sbjct: 121  KDFMSPWHHLKEGLVNNS-NAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALV 179

Query: 479  KECGNIFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGD 300
             ECGNIF                V+ ++ AFPHIKC++YDLPHVI+DSP    +  + G+
Sbjct: 180  NECGNIFQGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGE 239

Query: 299  MFKFIPKADAIMMKCILHDWDDKECI 222
            MFK IP ADAI+MKCILHDW+D ECI
Sbjct: 240  MFKSIPSADAILMKCILHDWNDDECI 265



 Score = 61.6 bits (148), Expect(2) = 9e-97
 Identities = 30/50 (60%), Positives = 37/50 (74%)
 Frame = -3

Query: 154 ECIEILKRCKEAXXXXXXXXXXXXXVLNVQSEHPYTKMRLTLDLDMMLNT 5
           ECIEILK+CKEA             VL+++S+HPY+K+RLTLDLDMMLNT
Sbjct: 263 ECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMMLNT 312


>gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum flavum]
          Length = 225

 Score =  355 bits (910), Expect = 1e-95
 Identities = 174/222 (78%), Positives = 191/222 (86%), Gaps = 2/222 (0%)
 Frame = -2

Query: 998 VKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVSE 819
           + K+NLSSQAKLWNFIYGFA+SLVLK AVQLDLANIIHN+G+ MTLSELS  LPSQPV++
Sbjct: 4   INKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQ 63

Query: 818 DALYRVMRYLVHMKLFTKESIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMAPW 639
           DALYRV+RYLVHMKLFTK SIDGELRYGLAPPAKFLVKGWDKCM+G+IL ITDKDFMAPW
Sbjct: 64  DALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPW 123

Query: 638 HYLKDGL--SGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 465
           HYLK+G+   G + TAFEKALGTNIW YMAEHPEKNQLFNE MAND+RLIMSALVKEC +
Sbjct: 124 HYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSS 183

Query: 464 IFNXXXXXXXXXXXXXXXVRNIANAFPHIKCTVYDLPHVIAD 339
           +F+               VRNIA AFPHIKCTVYDLPHVIAD
Sbjct: 184 MFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIAD 225