BLASTX nr result
ID: Coptis21_contig00016353
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00016353
(2199 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 856 0.0
emb|CBI20743.3| unnamed protein product [Vitis vinifera] 817 0.0
ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylas... 778 0.0
ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 778 0.0
ref|XP_002308509.1| predicted protein [Populus trichocarpa] gi|2... 777 0.0
>ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Vitis vinifera]
Length = 563
Score = 856 bits (2212), Expect = 0.0
Identities = 422/543 (77%), Positives = 484/543 (89%)
Frame = -1
Query: 2199 GEEKCCVVTGGRGFAARHLVEMLIHSKKWLVKIADLGPSIKLEPYEEQSILGEALRSGAA 2020
GE+K CVVTGGRGFAARHLVEMLI S ++ V+IADLGPSIKLEP EE+ +LGEAL+SG A
Sbjct: 3 GEQKWCVVTGGRGFAARHLVEMLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRA 62
Query: 2019 KYVSADLRDQSQVIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVK 1840
+YVSADLRD++QV+KA +GA+ VFHMAAPDSSINN QLH+SVNVQGTKNVIDAC+E KVK
Sbjct: 63 EYVSADLRDKAQVLKACQGAEAVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVK 122
Query: 1839 RLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVINSNGRNGLLTCCI 1660
RLIYTSSPSVVFDGVHGIFNGDESL YP KHNDSYSATKAEGEALVI SNG NGLLTCCI
Sbjct: 123 RLIYTSSPSVVFDGVHGIFNGDESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCI 182
Query: 1659 RPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEG 1480
RPSSIFGPGD+LLVPSLV AARAGKSKFIIGDGNN+YDFTYVENVAHAH+CAE AL+SEG
Sbjct: 183 RPSSIFGPGDKLLVPSLVNAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEG 242
Query: 1479 EVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVL 1300
++A++AAGQAYFITNMEPIKFWEFVS ILEGLGYERP IKIP VMMPIAH+VEWTYK+L
Sbjct: 243 KIAEQAAGQAYFITNMEPIKFWEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKML 302
Query: 1299 APYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQK 1120
APYGM+VPQLTPSRIRLLS SRTFSCSKA+D+L Y PIVSLQ+GL+RTI+SY LRAE
Sbjct: 303 APYGMKVPQLTPSRIRLLSCSRTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAEHL 362
Query: 1119 QRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYFYFIASGYTIITAVSQILL 940
+R GPSKAS YLG G++A+TLLWKDKK LT ++VL+AFY FIASGYTI+TAVS++ L
Sbjct: 363 PKREGPSKASRYLGSGRVADTLLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKLFL 422
Query: 939 MATLFLFIHGILPAKMLGYTIEKIPASNFHFPEKTSHQVALSVGSTWNYAVNVLKSLCKG 760
+A++FLFIHG LP K+LGYT+EKIP SNFHF E+ SH+ ALSV S+WN AVNVLKSLCKG
Sbjct: 423 VASIFLFIHGNLPEKILGYTVEKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLCKG 482
Query: 759 EDFVLFVKVVISLLICSFLGATSIGSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLLG 580
+D++LF+KVV+SLLI SFLGA S+ S+F+ G+ AFVAF++YE KEEEID L +AL
Sbjct: 483 DDWMLFLKVVLSLLILSFLGAISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFS 542
Query: 579 CKV 571
CK+
Sbjct: 543 CKL 545
>emb|CBI20743.3| unnamed protein product [Vitis vinifera]
Length = 540
Score = 817 bits (2111), Expect = 0.0
Identities = 403/522 (77%), Positives = 464/522 (88%)
Frame = -1
Query: 2136 MLIHSKKWLVKIADLGPSIKLEPYEEQSILGEALRSGAAKYVSADLRDQSQVIKAFEGAD 1957
MLI S ++ V+IADLGPSIKLEP EE+ +LGEAL+SG A+YVSADLRD++QV+KA +GA+
Sbjct: 1 MLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAE 60
Query: 1956 VVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKRLIYTSSPSVVFDGVHGIFNG 1777
VFHMAAPDSSINN QLH+SVNVQGTKNVIDAC+E KVKRLIYTSSPSVVFDGVHGIFNG
Sbjct: 61 AVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNG 120
Query: 1776 DESLAYPSKHNDSYSATKAEGEALVINSNGRNGLLTCCIRPSSIFGPGDRLLVPSLVTAA 1597
DESL YP KHNDSYSATKAEGEALVI SNG NGLLTCCIRPSSIFGPGD+LLVPSLV AA
Sbjct: 121 DESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAA 180
Query: 1596 RAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGEVAQRAAGQAYFITNMEPIKF 1417
RAGKSKFIIGDGNN+YDFTYVENVAHAH+CAE AL+SEG++A++AAGQAYFITNMEPIKF
Sbjct: 181 RAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKF 240
Query: 1416 WEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLAPYGMRVPQLTPSRIRLLSRS 1237
WEFVS ILEGLGYERP IKIP VMMPIAH+VEWTYK+LAPYGM+VPQLTPSRIRLLS S
Sbjct: 241 WEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCS 300
Query: 1236 RTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQKQRRVGPSKASIYLGRGKIANT 1057
RTFSCSKA+D+L Y PIVSLQ+GL+RTI+SY LRAE +R GPSKAS YLG G++A+T
Sbjct: 301 RTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAEHLPKREGPSKASRYLGSGRVADT 360
Query: 1056 LLWKDKKQTLTAVVVLIAFYFYFIASGYTIITAVSQILLMATLFLFIHGILPAKMLGYTI 877
LLWKDKK LT ++VL+AFY FIASGYTI+TAVS++ L+A++FLFIHG LP K+LGYT+
Sbjct: 361 LLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTV 420
Query: 876 EKIPASNFHFPEKTSHQVALSVGSTWNYAVNVLKSLCKGEDFVLFVKVVISLLICSFLGA 697
EKIP SNFHF E+ SH+ ALSV S+WN AVNVLKSLCKG+D++LF+KVV+SLLI SFLGA
Sbjct: 421 EKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFLKVVLSLLILSFLGA 480
Query: 696 TSIGSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLLGCKV 571
S+ S+F+ G+ AFVAF++YE KEEEID L +AL CK+
Sbjct: 481 ISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFSCKL 522
>ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
[Arabidopsis thaliana]
gi|229890388|sp|Q67ZE1.2|HSDD2_ARATH RecName:
Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 2; Short=At3BETAHSD/D2; AltName:
Full=4alpha-carboxysterol-C3-dehydrogenase/C4-
decarboxylase isoform 1-2; AltName: Full=Reticulon-like
protein B19; Short=AtRTNLB19; AltName:
Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 2,
decarboxylating gi|330252720|gb|AEC07814.1|
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2 [Arabidopsis thaliana]
Length = 564
Score = 778 bits (2008), Expect = 0.0
Identities = 379/540 (70%), Positives = 453/540 (83%)
Frame = -1
Query: 2193 EKCCVVTGGRGFAARHLVEMLIHSKKWLVKIADLGPSIKLEPYEEQSILGEALRSGAAKY 2014
E+ CVVTGGRGFAARHLVEML+ + + V+IADL P+I L+P E +L E LRSG +Y
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68
Query: 2013 VSADLRDQSQVIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKRL 1834
+SADLRD+SQV+KAF+GA+VVFHMAAPDSSINN QL YSVNVQGT+NVIDACV+ VKRL
Sbjct: 69 ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128
Query: 1833 IYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVINSNGRNGLLTCCIRP 1654
IYTSSPSVVFDGVHGI NG ES+AYP KHNDSYSATKAEGE L++ +NGRNGLLTCCIRP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188
Query: 1653 SSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGEV 1474
SSIFGPGDRLLVPSLV AARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAE AL+S G+V
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248
Query: 1473 AQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLAP 1294
+ +AAGQAYFITNMEPIKFWEF+S++L+GLGYERPSIKIP +MMPIAH+VE TYKVL P
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308
Query: 1293 YGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQKQR 1114
YGM VPQLTPSR+RLLS SRTF +KA+DRLGY P+V LQ+G++RTI+S+SHL A + +
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368
Query: 1113 RVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYFYFIASGYTIITAVSQILLMA 934
R GPSKAS LG GK+A+TLLWKD KQTL A+ +LI+ Y+ F+A+G T++TA+S+ LL+A
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428
Query: 933 TLFLFIHGILPAKMLGYTIEKIPASNFHFPEKTSHQVALSVGSTWNYAVNVLKSLCKGED 754
++FLF+HGILP K+ GYT+EKIPAS FH + +SH ++LSV S+WN V L+SLC+G D
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488
Query: 753 FVLFVKVVISLLICSFLGATSIGSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLLGCK 574
+ F KVV LL S GA S+ SIF+IG+P AF+AF +YEKKE+EID +V CK
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSFACK 548
>ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Glycine max]
Length = 563
Score = 778 bits (2008), Expect = 0.0
Identities = 386/544 (70%), Positives = 459/544 (84%), Gaps = 2/544 (0%)
Frame = -1
Query: 2196 EEKCCVVTGGRGFAARHLVEMLIHSKKWLVKIADLGPSIKLEPYEEQSILGEALRSGAAK 2017
E+K CVVTGGRGFAARHLVEMLI ++ V+IADL +I LEP E+ +LG+AL SG A+
Sbjct: 5 EDKWCVVTGGRGFAARHLVEMLIRHNEYCVRIADLEANIVLEPAEQLGLLGQALHSGRAQ 64
Query: 2016 YVSADLRDQSQVIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKR 1837
YVS DLR+++Q++KA EG +VVFHMAAP+SSINN QLH+SVNVQGTKNVIDACVE VKR
Sbjct: 65 YVSLDLRNKAQLLKALEGVEVVFHMAAPNSSINNYQLHHSVNVQGTKNVIDACVELNVKR 124
Query: 1836 LIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVINSNGRNGLLTCCIR 1657
L+YTSSPSVVFDGVHGI NG+E++ Y ND YSATKAEGEALVI +NG NGLLTCCIR
Sbjct: 125 LVYTSSPSVVFDGVHGIHNGNETMPYAHSPNDHYSATKAEGEALVIKANGTNGLLTCCIR 184
Query: 1656 PSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGE 1477
PSSIFGPGDRLLVPSLV AAR GKSKF+IGDGNN+YDFTYVENVAHAH+CA+ AL SE
Sbjct: 185 PSSIFGPGDRLLVPSLVDAARKGKSKFLIGDGNNVYDFTYVENVAHAHICADRALVSEAP 244
Query: 1476 VAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLA 1297
V+++AAG+AYFITNMEP+KFWEFVS ++EGLGYE P IKIP V+MP AH+VEW Y++L
Sbjct: 245 VSEKAAGEAYFITNMEPMKFWEFVSVVVEGLGYEGPRIKIPTFVIMPFAHLVEWIYRLLG 304
Query: 1296 PYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQ-- 1123
PYGM+VPQLTPSRIRL S SRTF CSKA+DRLGY PIV+LQ+GL+RTIESY+HL+AE
Sbjct: 305 PYGMKVPQLTPSRIRLTSCSRTFDCSKAKDRLGYAPIVTLQEGLRRTIESYTHLKAENQP 364
Query: 1122 KQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYFYFIASGYTIITAVSQIL 943
K +R G SKASIYLG G++A+TLLWKDKKQT A++VLIA YF FIAS TII+A++++L
Sbjct: 365 KTKREGSSKASIYLGSGRVADTLLWKDKKQTFIALLVLIAIYFNFIASENTIISALTKLL 424
Query: 942 LMATLFLFIHGILPAKMLGYTIEKIPASNFHFPEKTSHQVALSVGSTWNYAVNVLKSLCK 763
L A++FLFIHGILPAK+LGYT+EKIP S FH E SHQ+ALSV S+WN AVNVLKSL
Sbjct: 425 LFASIFLFIHGILPAKILGYTLEKIPKSWFHLSEDMSHQIALSVASSWNIAVNVLKSLAD 484
Query: 762 GEDFVLFVKVVISLLICSFLGATSIGSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLL 583
G D+VLF K V+SL I SFLGA S+ S++ IG+ FAF+AF++YE+KEEEI GL + L
Sbjct: 485 GNDWVLFFKAVLSLFILSFLGAFSLQSLYTIGVTFAFIAFYVYEQKEEEIGGLFIKTHSL 544
Query: 582 GCKV 571
GCK+
Sbjct: 545 GCKL 548
>ref|XP_002308509.1| predicted protein [Populus trichocarpa] gi|222854485|gb|EEE92032.1|
predicted protein [Populus trichocarpa]
Length = 558
Score = 777 bits (2006), Expect = 0.0
Identities = 389/543 (71%), Positives = 450/543 (82%)
Frame = -1
Query: 2199 GEEKCCVVTGGRGFAARHLVEMLIHSKKWLVKIADLGPSIKLEPYEEQSILGEALRSGAA 2020
GEE+ CVVTGGRGFAARHLVEMLI + V+IADL P I L E+ LG AL+SG A
Sbjct: 3 GEERWCVVTGGRGFAARHLVEMLIQYDLFSVRIADLEPQITLSTEEQNGALGHALKSGRA 62
Query: 2019 KYVSADLRDQSQVIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVK 1840
YVSADLRD+ QV+K EGA+VVFHMAAP+SS+N+QQLHYSVNVQGTKNVIDACVE KVK
Sbjct: 63 NYVSADLRDKPQVLKVIEGAEVVFHMAAPNSSVNSQQLHYSVNVQGTKNVIDACVELKVK 122
Query: 1839 RLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVINSNGRNGLLTCCI 1660
RLIYTSS SVVFDG+HGI NGDESL Y K DSYSATKAEGEA ++ +NG NGLLTC +
Sbjct: 123 RLIYTSSASVVFDGIHGIINGDESLPYTDKPLDSYSATKAEGEAAILKANGTNGLLTCSL 182
Query: 1659 RPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEG 1480
RPSSIFGPGDRL VPSLV AARAGKSKF+IGDGNN+YDFTYVENVAHAH+CA+ AL+SE
Sbjct: 183 RPSSIFGPGDRLFVPSLVAAARAGKSKFMIGDGNNIYDFTYVENVAHAHICADRALASEQ 242
Query: 1479 EVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVL 1300
EVA++AAGQAYFITNMEPIKFWEF S ILEGLGYERP IKIP +MPIA V+EW YK+
Sbjct: 243 EVAEKAAGQAYFITNMEPIKFWEFTSLILEGLGYERPRIKIPAFAVMPIAQVIEWIYKIF 302
Query: 1299 APYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQK 1120
PYGM+VPQL PSRIRLLS SR+F+CSKA+DRLGY PIV L++GLKRT+ESY HLRAE +
Sbjct: 303 GPYGMKVPQLIPSRIRLLSCSRSFNCSKAKDRLGYAPIVPLEEGLKRTLESYPHLRAENQ 362
Query: 1119 QRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYFYFIASGYTIITAVSQILL 940
+R GPSKASI LG GK +TLLWKDKKQTL ++VL+A Y+ FIAS TI+TA S++LL
Sbjct: 363 PKREGPSKASICLGSGK--DTLLWKDKKQTLITLLVLVAIYYNFIASQSTIVTAASKLLL 420
Query: 939 MATLFLFIHGILPAKMLGYTIEKIPASNFHFPEKTSHQVALSVGSTWNYAVNVLKSLCKG 760
+ ++FLFIHG LP K+ GYTIEKIP S F+ E+ SHQ ALSV S+WN AVNVLKSLCKG
Sbjct: 421 VVSVFLFIHGNLPEKVFGYTIEKIPMSYFNLSEERSHQTALSVASSWNAAVNVLKSLCKG 480
Query: 759 EDFVLFVKVVISLLICSFLGATSIGSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLLG 580
D+ LF +VVISLLI SFLGA S ++F+IG+P AF AF+LYEK E+ ID + +AL LG
Sbjct: 481 NDWSLFFQVVISLLILSFLGAISFQTLFIIGLPVAFAAFYLYEKNEQAIDSMFLEALSLG 540
Query: 579 CKV 571
CK+
Sbjct: 541 CKM 543