BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00016115
         (1256 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABS57468.1| caffeic acid O-methyl transferase [Leucaena leuco...   347   4e-93
gb|AAF60951.1| O-methyltransferase [Populus trichocarpa x Populu...   343   5e-92
gb|ABU53653.1| caffeic acid O-3-methyltransferase [Populus delto...   343   7e-92
sp|Q00763.1|COMT1_POPTM RecName: Full=Caffeic acid 3-O-methyltra...   342   1e-91
prf||1906376A O-methyltransferase                                     342   1e-91

>gb|ABS57468.1| caffeic acid O-methyl transferase [Leucaena leucocephala]
            gi|155966780|gb|ABU41320.1| caffeic acid o-methyl
            transferase [Leucaena leucocephala]
          Length = 365

 Score =  347 bits (890), Expect = 4e-93
 Identities = 172/364 (47%), Positives = 240/364 (65%)
 Frame = +2

Query: 38   SSGENQFISTNNGEVDDDYTPAVYLTTLSVLPMVMRSVIELEVLDIIARAGPDAQLSASQ 217
            S+GE Q I T+  + + +   A+ L + SVLPM+++S +EL++L+IIA+AGP+AQLS S 
Sbjct: 3    STGETQIIPTHVNDEEANLF-AMQLASASVLPMILKSALELDLLEIIAKAGPNAQLSPSN 61

Query: 218  ITSHIPTRKPSEXXXXXXXXXXXXXSYSFVSCSTVSDENGHTERLYGLVPISKSFVLDQD 397
            I + +PT+ P +              Y+ +SCS  +  +G  ERLYGL P++K  V ++D
Sbjct: 62   IAAQLPTKNP-DAAVMLDRMMRLLACYNVLSCSVRTLPDGKIERLYGLAPVAKYLVKNED 120

Query: 398  GLSMSPMLMMVGDKVVMETWYHLKDAILDGGIPFNKAHGIESYRYPSIDERFNNVFNKAM 577
            G+S++P+ +M  DKV+ME+WY+LKDA+L+GGIPFNKAHG+ S+ Y   D RFN VFNK M
Sbjct: 121  GVSIAPLNLMNQDKVLMESWYYLKDAVLEGGIPFNKAHGMTSFEYHGKDLRFNKVFNKGM 180

Query: 578  FNSTYIFMNKIVDAYKGFENVERVVDVXXXXXXXXXXXXXRYPAIKGINFDLPHVVQTAP 757
             + + I M KI++ Y GFE +  +VDV             +YP+I GINFDLPHV++ AP
Sbjct: 181  ADHSTITMKKILETYTGFEGLTSLVDVGGGTGAVISMIVSKYPSINGINFDLPHVIEDAP 240

Query: 758  SYPGVEHVGGDMFASIPKCDAIFLKGIIHDWSDEHCIKFLKNCHKALPKNGKXXXXXXXX 937
            SYPGVEHVGGDMF S+PK DA+F+K I HDWSDEHC+KFLKNC+ ALP+NGK        
Sbjct: 241  SYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDEHCVKFLKNCYDALPENGKVIVAECIL 300

Query: 938  XXXXXXXXXXKELFQFDLLMMVNHPGGKERTEDELQAIAKKVGFADATIALRPFKYWVVM 1117
                      K +   D++M+ ++PGGKERTE E +A+AK  GF    +    F  + +M
Sbjct: 301  PASPDSSLATKGVVHIDVIMLAHNPGGKERTEKEFEALAKAAGFQGFRVCCSAFNTY-IM 359

Query: 1118 EFIK 1129
            EF+K
Sbjct: 360  EFLK 363


>gb|AAF60951.1| O-methyltransferase [Populus trichocarpa x Populus deltoides]
          Length = 364

 Score =  343 bits (880), Expect = 5e-92
 Identities = 169/359 (47%), Positives = 232/359 (64%)
 Frame = +2

Query: 38   SSGENQFISTNNGEVDDDYTPAVYLTTLSVLPMVMRSVIELEVLDIIARAGPDAQLSASQ 217
            S+GE Q   T   + ++ +  A+ L + SVLPM++++ IEL++L+I+A+AGP A LS S+
Sbjct: 3    STGETQMTPTQVSD-EEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSE 61

Query: 218  ITSHIPTRKPSEXXXXXXXXXXXXXSYSFVSCSTVSDENGHTERLYGLVPISKSFVLDQD 397
            I SH+PT+ P +             SYS ++CS     +G  ERLYGL P+ K    ++D
Sbjct: 62   IASHLPTKNP-DAPVMLDRILRLLASYSILTCSLKDHPDGKVERLYGLAPVCKFLTKNED 120

Query: 398  GLSMSPMLMMVGDKVVMETWYHLKDAILDGGIPFNKAHGIESYRYPSIDERFNNVFNKAM 577
            G+S+SP+ +M  DKV+ME+WY+LKDAILDGGIPFNKA+G+ ++ Y   D RFN VFNK M
Sbjct: 121  GVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGM 180

Query: 578  FNSTYIFMNKIVDAYKGFENVERVVDVXXXXXXXXXXXXXRYPAIKGINFDLPHVVQTAP 757
             + + I M KI++ YKGFE +  +VDV             +YP+IKGINFDLPHV++ AP
Sbjct: 181  SDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAP 240

Query: 758  SYPGVEHVGGDMFASIPKCDAIFLKGIIHDWSDEHCIKFLKNCHKALPKNGKXXXXXXXX 937
            SYPGVEHVGGDMF S+PK DA+F+K I HDWSD HC+KFLKNC+ ALP+NGK        
Sbjct: 241  SYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDAHCLKFLKNCYDALPENGKVILVECIL 300

Query: 938  XXXXXXXXXXKELFQFDLLMMVNHPGGKERTEDELQAIAKKVGFADATIALRPFKYWVV 1114
                      K +   D++M+ ++PGGKERTE E + +AK  GF    +    F   V+
Sbjct: 301  PVAPDTSLATKGVVHIDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTHVI 359


>gb|ABU53653.1| caffeic acid O-3-methyltransferase [Populus deltoides]
          Length = 364

 Score =  343 bits (879), Expect = 7e-92
 Identities = 169/359 (47%), Positives = 232/359 (64%)
 Frame = +2

Query: 38   SSGENQFISTNNGEVDDDYTPAVYLTTLSVLPMVMRSVIELEVLDIIARAGPDAQLSASQ 217
            S+GE Q   T   + ++ +  A+ L + SVLPM++++ IEL++L+I+A+AGP A LS S+
Sbjct: 3    STGETQMTPTQVSD-EEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSE 61

Query: 218  ITSHIPTRKPSEXXXXXXXXXXXXXSYSFVSCSTVSDENGHTERLYGLVPISKSFVLDQD 397
            I SH+PT+ P +             SYS ++CS     +G  ERLYGL P+ K    ++D
Sbjct: 62   IASHLPTKNP-DAPVMLDRILRLLASYSILTCSLKDHPDGKVERLYGLAPVCKFLTKNED 120

Query: 398  GLSMSPMLMMVGDKVVMETWYHLKDAILDGGIPFNKAHGIESYRYPSIDERFNNVFNKAM 577
            G+S+SP+ +M  DKV+ME+WY+LKDAILDGGIPFNKA+G+ ++ Y   D RFN VFNK M
Sbjct: 121  GVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGM 180

Query: 578  FNSTYIFMNKIVDAYKGFENVERVVDVXXXXXXXXXXXXXRYPAIKGINFDLPHVVQTAP 757
             + + I M KI++ YKGFE +  +VDV             +YP+IKGINFDLPHV++ AP
Sbjct: 181  SDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAP 240

Query: 758  SYPGVEHVGGDMFASIPKCDAIFLKGIIHDWSDEHCIKFLKNCHKALPKNGKXXXXXXXX 937
            SYPGVEHVGGDMF S+PK DA+F+K I HDWSD HC+KFLKNC+ ALP+NGK        
Sbjct: 241  SYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDVHCLKFLKNCYDALPENGKVILVECIL 300

Query: 938  XXXXXXXXXXKELFQFDLLMMVNHPGGKERTEDELQAIAKKVGFADATIALRPFKYWVV 1114
                      K +   D++M+ ++PGGKERTE E + +AK  GF    +    F   V+
Sbjct: 301  PVAPDTSLATKGVVHIDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTHVI 359


>sp|Q00763.1|COMT1_POPTM RecName: Full=Caffeic acid 3-O-methyltransferase 1; Short=CAOMT-1;
            Short=COMT-1; AltName:
            Full=S-adenosysl-L-methionine:caffeic acid
            3-O-methyltransferase 1 gi|20951|emb|CAA44006.1| lignin
            bispecific acid/5-hydroxyferulic acid methyltransferase
            [Populus tremuloides] gi|2226267|gb|AAB61731.1| caffeic
            acid/5-hydroxyferulic acid O-methyltransferase [Populus
            tremuloides]
          Length = 365

 Score =  342 bits (877), Expect = 1e-91
 Identities = 172/364 (47%), Positives = 235/364 (64%)
 Frame = +2

Query: 38   SSGENQFISTNNGEVDDDYTPAVYLTTLSVLPMVMRSVIELEVLDIIARAGPDAQLSASQ 217
            S+GE Q   T   + ++ +  A+ L + SVLPM++++ IEL++L+I+A+AGP A LS S+
Sbjct: 3    STGETQMTPTQVSD-EEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSE 61

Query: 218  ITSHIPTRKPSEXXXXXXXXXXXXXSYSFVSCSTVSDENGHTERLYGLVPISKSFVLDQD 397
            I SH+PT+ P +             SYS ++CS     +G  ERLYGL P+ K    ++D
Sbjct: 62   IASHLPTKNP-DAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPVCKFLTKNED 120

Query: 398  GLSMSPMLMMVGDKVVMETWYHLKDAILDGGIPFNKAHGIESYRYPSIDERFNNVFNKAM 577
            G+S+SP+ +M  DKV+ME+WY+LKDAILDGGIPFNKA+G+ ++ Y   D RFN VFNK M
Sbjct: 121  GVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGM 180

Query: 578  FNSTYIFMNKIVDAYKGFENVERVVDVXXXXXXXXXXXXXRYPAIKGINFDLPHVVQTAP 757
             + + I M KI++ YKGFE +  +VDV             +YP+IKGINFDLPHV++ AP
Sbjct: 181  SDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAP 240

Query: 758  SYPGVEHVGGDMFASIPKCDAIFLKGIIHDWSDEHCIKFLKNCHKALPKNGKXXXXXXXX 937
            SYPGVEHVGGDMF S+PK DA+F+K I HDWSD HC+KFLKNC+ ALP+NGK        
Sbjct: 241  SYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDAHCLKFLKNCYDALPENGKVILVECIL 300

Query: 938  XXXXXXXXXXKELFQFDLLMMVNHPGGKERTEDELQAIAKKVGFADATIALRPFKYWVVM 1117
                      K +   D++M+ ++PGGKERTE E + +AK  GF    +    F   V+ 
Sbjct: 301  PVAPDTSLATKGVVHVDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTHVI- 359

Query: 1118 EFIK 1129
            EF K
Sbjct: 360  EFRK 363


>prf||1906376A O-methyltransferase
          Length = 364

 Score =  342 bits (877), Expect = 1e-91
 Identities = 169/359 (47%), Positives = 231/359 (64%)
 Frame = +2

Query: 38   SSGENQFISTNNGEVDDDYTPAVYLTTLSVLPMVMRSVIELEVLDIIARAGPDAQLSASQ 217
            S+GE Q   T   + ++ +  A+ L + SVLPM++++ IEL++L+I+A+AGP A LS S+
Sbjct: 3    STGETQMTPTQVSD-EEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSE 61

Query: 218  ITSHIPTRKPSEXXXXXXXXXXXXXSYSFVSCSTVSDENGHTERLYGLVPISKSFVLDQD 397
            I SH+PT+ P +             SYS + CS     +G  ERLYGL P+ K    ++D
Sbjct: 62   IASHLPTKNP-DAPVMLDRILRLLASYSILICSLKDHPDGKVERLYGLAPVCKFLTKNED 120

Query: 398  GLSMSPMLMMVGDKVVMETWYHLKDAILDGGIPFNKAHGIESYRYPSIDERFNNVFNKAM 577
            G+S+SP+ +M  DKV+ME+WY+LKDAILDGGIPFNKA+G+ ++ Y   D RFN VFNK M
Sbjct: 121  GVSVSPLCLMNQDKVLMESWYYLKDAILDGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGM 180

Query: 578  FNSTYIFMNKIVDAYKGFENVERVVDVXXXXXXXXXXXXXRYPAIKGINFDLPHVVQTAP 757
             + + I M KI++ YKGFE +  +VDV             +YP+IKGINFDLPHV++ AP
Sbjct: 181  SDHSTITMKKILETYKGFEGLTSLVDVGGGTGAVVNTIVSKYPSIKGINFDLPHVIEDAP 240

Query: 758  SYPGVEHVGGDMFASIPKCDAIFLKGIIHDWSDEHCIKFLKNCHKALPKNGKXXXXXXXX 937
            SYPGVEHVGGDMF S+PK DA+F+K I HDWSD HC+KFLKNC+ ALP+NGK        
Sbjct: 241  SYPGVEHVGGDMFVSVPKADAVFMKWICHDWSDAHCLKFLKNCYDALPENGKVILVECIL 300

Query: 938  XXXXXXXXXXKELFQFDLLMMVNHPGGKERTEDELQAIAKKVGFADATIALRPFKYWVV 1114
                      K +   D++M+ ++PGGKERTE E + +AK  GF    +    F   V+
Sbjct: 301  PVAPDTSLATKGVVHIDVIMLAHNPGGKERTEKEFEGLAKGAGFQGFEVMCCAFNTHVI 359