BLASTX nr result
ID: Coptis21_contig00013631
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00013631
(1654 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 851 0.0
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 812 0.0
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 722 0.0
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 645 0.0
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 643 0.0
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 851 bits (2198), Expect = 0.0
Identities = 438/488 (89%), Positives = 438/488 (89%)
Frame = -1
Query: 1603 MEVLSIAIVSXXXXXXXXXXLRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 1424
MEVLSIAIVS LRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY
Sbjct: 1 MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60
Query: 1423 GELFSLKLGSQTXXXXXXXXXXAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 1244
GELFSLKLGSQT AEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN
Sbjct: 61 GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 1243 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRXXXXXXXXXXXXVFGTLVNIFGN 1064
WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLR VFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180
Query: 1063 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 884
LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 240
Query: 883 QIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 704
QIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW
Sbjct: 241 QIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 300
Query: 703 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXX 524
AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 523 XXXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 344
TCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
Query: 343 GGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 164
GGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV
Sbjct: 421 GGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 480
Query: 163 LKARASNI 140
LKARASNI
Sbjct: 481 LKARASNI 488
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 812 bits (2097), Expect = 0.0
Identities = 417/488 (85%), Positives = 425/488 (87%)
Frame = -1
Query: 1603 MEVLSIAIVSXXXXXXXXXXLRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 1424
MEV+SIAIVS LRD R KNLPPGPRP PIVGNLLQLGDKP AEFAKLAQKY
Sbjct: 1 MEVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKY 60
Query: 1423 GELFSLKLGSQTXXXXXXXXXXAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 1244
G+LFSLKLGSQT AE+LKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN
Sbjct: 61 GQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 1243 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRXXXXXXXXXXXXVFGTLVNIFGN 1064
WKLLRKVCRTELFTPKMIESQSEIRE KAREMVKFLR VFGTLVNIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180
Query: 1063 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 884
LIFSKDVFDLEDPTGGSVELKEHLWKLL+MGNSTNPADYFPIMGK DLFGQRRAVA+VLQ
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQ 240
Query: 883 QIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 704
QIYDVWG+ LKERRG KGSES NDFVDVLLNAGL+DQKINALLMELFG GTETSASTIEW
Sbjct: 241 QIYDVWGINLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEW 300
Query: 703 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXX 524
AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRR 360
Query: 523 XXXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 344
TCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF
Sbjct: 361 ALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420
Query: 343 GGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 164
GGGRRICPGLPLASQF +LIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV
Sbjct: 421 GGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIV 480
Query: 163 LKARASNI 140
LKAR S I
Sbjct: 481 LKARPSYI 488
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 722 bits (1863), Expect = 0.0
Identities = 372/487 (76%), Positives = 400/487 (82%), Gaps = 1/487 (0%)
Frame = -1
Query: 1603 MEVLSIAIVSXXXXXXXXXXLR-DSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQK 1427
MEVLS A+VS + DSR K+LPPGPRPSPIVGNLLQLG+KPHAEFAKLA+K
Sbjct: 1 MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60
Query: 1426 YGELFSLKLGSQTXXXXXXXXXXAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECND 1247
YGELF+LKLGSQT AEILKT DKILSGRYVFQSFRV EHV NSIVWSECN+
Sbjct: 61 YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120
Query: 1246 NWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRXXXXXXXXXXXXVFGTLVNIFG 1067
NWKLLRKVCRTELF+PKMIESQ+ IREAKA +MV+FLR VF TLVNIFG
Sbjct: 121 NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180
Query: 1066 NLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVL 887
NLIFSKDVFDLEDPTGGS ELKEHLWKLLDMGNSTNPADYFPIMGK DLFGQRR VA+VL
Sbjct: 181 NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240
Query: 886 QQIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIE 707
+QIYDVWGVMLKERR G +NDF +VLLNAGLDDQKINALLMELFGAGTET+ASTIE
Sbjct: 241 KQIYDVWGVMLKERRSITG-HRENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIE 299
Query: 706 WAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXX 527
WAITELT V+SK+R EL+NVVG TVKES++PHLPYLQA VKE
Sbjct: 300 WAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPR 359
Query: 526 XXXXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIP 347
TC VMNYTIPKECQIMVNAWAIGRDPKTWDDPL FKPERF++S VDYKGNDFELIP
Sbjct: 360 RALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIP 419
Query: 346 FGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLI 167
FGGGRRICPGLPLASQF +LIVATLVQNF+WS PQGM+ +E+ MDEKFGL LQKDPPLLI
Sbjct: 420 FGGGRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLI 479
Query: 166 VLKARAS 146
VLKAR S
Sbjct: 480 VLKARTS 486
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 645 bits (1663), Expect = 0.0
Identities = 313/465 (67%), Positives = 374/465 (80%), Gaps = 2/465 (0%)
Frame = -1
Query: 1540 RDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSLKLGSQTXXXXXXXXX 1361
RDS PK LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG+LFSLKLGS+T
Sbjct: 16 RDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLA 75
Query: 1360 XAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQ 1181
+EILKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+ WK LRKVCRTELFT KMIESQ
Sbjct: 76 ASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQ 135
Query: 1180 SEIREAKAREMVKFLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELK 1001
+E+RE+KA EMV++L+ VFGTLVNIFGNLIFS+++F L D + GSVE+K
Sbjct: 136 AEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMK 195
Query: 1000 EHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTK--GS 827
EHLW++L++GNSTNPADYFP +GK DLFGQ + VA+ LQ IY VWG MLKER+ K +
Sbjct: 196 EHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNN 255
Query: 826 ESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLEL 647
KNDFV++LL++GLDDQ+INALLME+FGAGTETSASTIEWA++ELTK P V + +RLEL
Sbjct: 256 SKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLEL 315
Query: 646 VNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXTCTVMNYTIPKECQIM 467
++VVG VKESD+P++PYLQAFVKE TC V+NYTIPKECQIM
Sbjct: 316 LSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIM 375
Query: 466 VNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNL 287
VNAW IGRDPK W DPL F PERFL+S +D+KGNDFELIPFG GRRICPG+PLA+QF +L
Sbjct: 376 VNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISL 435
Query: 286 IVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 152
IV++LVQNF+W P+GM S+L M+EKFGLTLQK+PPL IV K R
Sbjct: 436 IVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 643 bits (1659), Expect = 0.0
Identities = 315/483 (65%), Positives = 380/483 (78%), Gaps = 2/483 (0%)
Frame = -1
Query: 1594 LSIAIVSXXXXXXXXXXLRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGEL 1415
+++++V+ RDS PK LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG+L
Sbjct: 3 VTVSLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDL 62
Query: 1414 FSLKLGSQTXXXXXXXXXXAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKL 1235
F+LKLGS+T +EILKTHD++LSGRYVFQSFRVKEHVENSIVWSECN+ WK
Sbjct: 63 FTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKK 122
Query: 1234 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRXXXXXXXXXXXXVFGTLVNIFGNLIF 1055
LRKVCR ELFT KMIESQ+EIRE+KA EMV+FL+ VFGTLVNIFGNLIF
Sbjct: 123 LRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIF 182
Query: 1054 SKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIY 875
S+++F L D + GSVE+KEHLW++L++GNSTNPADYFP +G+ DLFGQR+ VA+ LQ IY
Sbjct: 183 SQNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 242
Query: 874 DVWGVMLKERRGTK--GSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWA 701
VWG MLKER+ K + KNDFV++LL++GLDDQ+INALLME+FGAGTETSASTIEWA
Sbjct: 243 SVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWA 302
Query: 700 ITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXX 521
++ELTK P V + +R EL++VVG VKESD+P++PYLQAFVKE
Sbjct: 303 LSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRA 362
Query: 520 XXTCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFG 341
TC V+NYTIPKECQIMVNAW IGRDPK W DPL F PERFL+S +D+KGNDFELIPFG
Sbjct: 363 LETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPFG 422
Query: 340 GGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVL 161
GRRICPG+PLA+QF +LIV TLVQNF+W LP+GM S+L M+EKFGLTLQK+PPL IV
Sbjct: 423 AGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVP 482
Query: 160 KAR 152
K R
Sbjct: 483 KNR 485