BLASTX nr result
ID: Coptis21_contig00012825
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00012825
(1969 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002269633.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 625 e-176
ref|XP_004147660.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 582 e-163
ref|XP_002529475.1| hydroxysteroid dehydrogenase, putative [Rici... 579 e-162
ref|XP_004160650.1| PREDICTED: LOW QUALITY PROTEIN: 3beta-hydrox... 578 e-162
ref|XP_003539040.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 570 e-160
>ref|XP_002269633.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Vitis vinifera] gi|297743842|emb|CBI36725.3|
unnamed protein product [Vitis vinifera]
Length = 581
Score = 625 bits (1613), Expect = e-176
Identities = 323/575 (56%), Positives = 409/575 (71%), Gaps = 1/575 (0%)
Frame = -2
Query: 1911 NNNSKTICVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSG 1732
+ N KT CVV GGR FIG+ LV LLK KWIVR+ADS L LD E S+LSE+ISSG
Sbjct: 4 DENLKT-CVVFGGRGFIGRFLVLRLLKLGKWIVRVADSAQSLQLDPSEDRSVLSEAISSG 62
Query: 1731 IVSYFQLDLHDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIRVCHECK 1552
S +D+ DK+Q+ KA++G++VV++ T + FY Y+ VQG +NVI C ECK
Sbjct: 63 RASCCAVDVRDKAQVRKAIEGASVVFYMDPTITYTNDFYLCYMIIVQGVRNVINACQECK 122
Query: 1551 VKRLIYTSSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQEEALVLFANCSDGLLTT 1372
VKRLIY SSADVVFDG HD+ NG+ESLP P +F D L+D+KAQ E LVL AN DGL+T
Sbjct: 123 VKRLIYNSSADVVFDGSHDIYNGDESLPCPWKFEDMLTDIKAQAEGLVLIANNIDGLVTC 182
Query: 1371 VLRPSNVFGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRS 1192
LRP NVFGPGD +LVPFL+ AKSG AKF++G+GEN+ DFTYV+NVAHAHVCAEEAL S
Sbjct: 183 ALRPCNVFGPGDKQLVPFLVNKAKSGHAKFVIGSGENMSDFTYVENVAHAHVCAEEALVS 242
Query: 1191 RTAFVAGKAFFITNLDPMKFWDFVSLIFKGLGYRRPRFKVPAKLFLFVAVLSNWLLEKLE 1012
R VAGK FFITNL+P+KFW+FVSLI +GLGY+RP FK+PA + L+V L W+ +KL+
Sbjct: 243 RMVSVAGKVFFITNLEPVKFWEFVSLILEGLGYQRPLFKLPAGMVLYVLSLVEWMRDKLD 302
Query: 1011 QRDAHSVLTPAIVRQLSCSMTFNCSEARNHIGYSPIVSLQEGVTVTIESFSHAAEDSVEF 832
R H V +V+ S + TFNCS A+ HIGYSP+VSL+EGVT+T ESFS ++DS
Sbjct: 303 FRSNHPVSAQYVVQIASRTRTFNCSAAQKHIGYSPVVSLEEGVTLTTESFSQLSQDSSVM 362
Query: 831 RYEDCNELSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKL 652
D +E SK D LG G+VADILLWRDEKKTFT FL L L+FYWFFL GRTF+SSAAKL
Sbjct: 363 ALRDFDEQSKVDKLLGGGKVADILLWRDEKKTFTCFLALFLMFYWFFLCGRTFISSAAKL 422
Query: 651 LLLISVVLFSHGILPASLFGYTVKKISPSHVEVAEK-VTDAIMYLASAWNHCMRTLSLLG 475
LLL++ +L GILP ++FG+++++IS S E+++ V D I +A+ WN+ + + LL
Sbjct: 423 LLLVTAILSGQGILPTNIFGFSIQRISLSCFEISDTVVNDLITNMANLWNNWIHMIRLLA 482
Query: 474 RGGDWNIFLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSST 295
GGDWN F KV LY LKL+ S + V GVALV AFT+FF+Y QYE+++D + E
Sbjct: 483 GGGDWNTFFKVAIPLYFLKLILSQCLTLVVGVALVLAFTSFFVYEQYEEEMDGLAEVLFD 542
Query: 294 GIKSLKMLLLRNVPVSVASLLDKSEIMGPKE*FPM 190
IK K LL+RN+P + S L + + E F M
Sbjct: 543 SIKKSKRLLMRNLPAPLVSFLCNRDALPDDEGFTM 577
>ref|XP_004147660.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Cucumis sativus]
Length = 582
Score = 582 bits (1499), Expect = e-163
Identities = 297/559 (53%), Positives = 385/559 (68%), Gaps = 1/559 (0%)
Frame = -2
Query: 1905 NSKTICVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSGIV 1726
N+KT CVVLGGR F+G+ LV LLK KW VRIADS L LD E S+LS+++ +G
Sbjct: 11 NTKT-CVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLLSDALIAGRA 69
Query: 1725 SYFQLDLHDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIRVCHECKVK 1546
SYF +D+ +K QI+ A+ GS+VV++ S Y Y+ VQG KNVI C +CKV+
Sbjct: 70 SYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVISACRDCKVR 129
Query: 1545 RLIYTSSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQEEALVLFANCSDGLLTTVL 1366
RLI+ SSADV+FDG D+LNG ESL YP +F D LSDLKAQ EAL+LFAN DGLLT L
Sbjct: 130 RLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCAL 189
Query: 1365 RPSNVFGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRT 1186
RPSNVFGP D +LVPF++ AKSG AKFI+G GEN+ DFT+V+NV HAH+CAEEAL R
Sbjct: 190 RPSNVFGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRM 249
Query: 1185 AFVAGKAFFITNLDPMKFWDFVSLIFKGLGYRRPRFKVPAKLFLFVAVLSNWLLEKLEQR 1006
VAGKAFFITN PMKFW+F+SLI +GLGYRRP K+P+K+ V + W+ EK
Sbjct: 250 VSVAGKAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMKWIDEKFGFN 309
Query: 1005 DAHSVLTPAIVRQLSCSMTFNCSEARNHIGYSPIVSLQEGVTVTIESFSHAAEDSVEFRY 826
+ ++ SC+ TF+C+ A +GYSP+VSL+EG+T+TI+SFSH ++ +
Sbjct: 310 KYNHSTWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHLRKELSLASF 369
Query: 825 EDCNELSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLL 646
+ E SKAD LG GRVAD+LLWRDEKK+FT FL LLFYWFF+ G TF+SSAA+LLL
Sbjct: 370 TEFTEESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFVRGGTFISSAAQLLL 429
Query: 645 LISVVLFSHGILPASLFGYTVKKISPSHVEVAEKVT-DAIMYLASAWNHCMRTLSLLGRG 469
I +VL+ +G +P +++G+ V+K+S + ++ V D+IM LA WN + L RG
Sbjct: 430 SIIIVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHNARALARG 489
Query: 468 GDWNIFLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGI 289
DWN FLK + LY+LKLL + S + + GV LVFAFTAFF+Y QYE ++D +F GI
Sbjct: 490 EDWNYFLKAVAFLYLLKLLLARSLTMLLGVGLVFAFTAFFVYEQYEAEIDEFAKFFFIGI 549
Query: 288 KSLKMLLLRNVPVSVASLL 232
LK LL ++P + S L
Sbjct: 550 MELKKLLASHLPTPLMSFL 568
>ref|XP_002529475.1| hydroxysteroid dehydrogenase, putative [Ricinus communis]
gi|223531033|gb|EEF32885.1| hydroxysteroid dehydrogenase,
putative [Ricinus communis]
Length = 579
Score = 579 bits (1492), Expect = e-162
Identities = 300/565 (53%), Positives = 391/565 (69%), Gaps = 2/565 (0%)
Frame = -2
Query: 1890 CVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKT-SILSESISSGIVSYFQ 1714
C V+GGR FIG+ LV LLK WIVR+ DS L LDL + S L+++ISSG SY
Sbjct: 15 CTVVGGRGFIGRSLVFNLLKLGNWIVRVVDSAQSLQLDLTDSLDSSLNDAISSGRASYHY 74
Query: 1713 LDLHDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIRVCHECKVKRLIY 1534
+D+ D S I KA++GS+VV++ AT + FY Y VQG KNVI C +CKV++LIY
Sbjct: 75 IDVIDISVIAKAIEGSSVVFYMEATDLWTHDFYYCYRNVVQGAKNVIDACRQCKVRKLIY 134
Query: 1533 TSSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQEEALVLFANCSDGLLTTVLRPSN 1354
SSADV+FDG HD+ NG+ESLP RF D L+DLKA EALVLFAN DGLLT LRPSN
Sbjct: 135 NSSADVIFDGSHDICNGDESLPCHWRFEDMLNDLKAHAEALVLFANNIDGLLTCALRPSN 194
Query: 1353 VFGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRTAFVA 1174
VFGPGD +L+PFL+ AKSG AKFI+G+GEN+ DFTYV+NVAHAH+CA EAL SR VA
Sbjct: 195 VFGPGDTQLLPFLISLAKSGCAKFIIGSGENMSDFTYVENVAHAHICAAEALDSRMVCVA 254
Query: 1173 GKAFFITNLDPMKFWDFVSLIFKGLGYRRPRFKVPAKLFLFVAVLSNWLLEKLEQRDAHS 994
GKAFFITNL+PMKFW+F SLI + L Y+RP K+PA++ +V + W+ EKL +
Sbjct: 255 GKAFFITNLEPMKFWEFASLILEALSYQRPLIKLPAQMVWYVLLFIKWMHEKLGFSKYNH 314
Query: 993 VLTPAIVRQLSCSMTFNCSEARNHIGYSPIVSLQEGVTVTIESFSHAAEDSVEFRYEDCN 814
++ R S + TFNC A+ +IGYSP+VSL +G+ +TIE++S+ +D R +
Sbjct: 315 SMSAYFFRLASHTRTFNCIAAQKYIGYSPVVSLDDGIALTIEAYSNLVKDPSFMRCPNFE 374
Query: 813 ELSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLLLISV 634
E SK D LG G+VADILLWRDEKKTFTYFL +LFYWF LSGRTF SSAA+LLLL
Sbjct: 375 EESKVDKLLGGGKVADILLWRDEKKTFTYFLIFAVLFYWFLLSGRTFTSSAARLLLLSVT 434
Query: 633 VLFSHGILPASLFGYTVKKISPSHVEVAE-KVTDAIMYLASAWNHCMRTLSLLGRGGDWN 457
+L+ +G LP + G+T+++IS S E++E V D++ +AS WN + + LL +G D N
Sbjct: 435 ILYGYGFLPLKISGFTIQRISLSWFEISETMVKDSVTSIASLWNRGVHNIRLLVQGKDSN 494
Query: 456 IFLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGIKSLK 277
FLKV+ LY +KL+ SF+ V G+ LVFAFTAFF+Y QYE +VD + + +GI+ K
Sbjct: 495 RFLKVLFVLYSIKLILLQSFTVVAGITLVFAFTAFFVYEQYEAEVDGLMKLLLSGIEESK 554
Query: 276 MLLLRNVPVSVASLLDKSEIMGPKE 202
LL+R +P VAS L ++ ++
Sbjct: 555 RLLMRKLPAPVASFLQNKGMLNKED 579
>ref|XP_004160650.1| PREDICTED: LOW QUALITY PROTEIN:
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Cucumis sativus]
Length = 582
Score = 578 bits (1491), Expect = e-162
Identities = 296/559 (52%), Positives = 383/559 (68%), Gaps = 1/559 (0%)
Frame = -2
Query: 1905 NSKTICVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEKTSILSESISSGIV 1726
N+KT CVVLGGR F+G+ LV LLK KW VRIADS L LD E S+LS+++ +G
Sbjct: 11 NTKT-CVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLLSDALIAGRA 69
Query: 1725 SYFQLDLHDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGTKNVIRVCHECKVK 1546
SYF +D+ +K QI+ A+ GS+VV++ S Y Y+ VQG KNVI C +CKV+
Sbjct: 70 SYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVISACRDCKVR 129
Query: 1545 RLIYTSSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQEEALVLFANCSDGLLTTVL 1366
RLI+ SSADV+FDG D+LNG ESL YP +F D LSDLKAQ EAL+LFAN DGLLT L
Sbjct: 130 RLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCAL 189
Query: 1365 RPSNVFGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAHAHVCAEEALRSRT 1186
RPSNVFGP D +LVPF++ AKSG AKFI+G GEN+ DFT+V+NV HAH+CAEEAL R
Sbjct: 190 RPSNVFGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHICAEEALDFRM 249
Query: 1185 AFVAGKAFFITNLDPMKFWDFVSLIFKGLGYRRPRFKVPAKLFLFVAVLSNWLLEKLEQR 1006
VAGKAFFITN PMKFW+F+SLI +GLGYRRP K+P+K+ V + W+ EK
Sbjct: 250 VSVAGKAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMKWIDEKFGFN 309
Query: 1005 DAHSVLTPAIVRQLSCSMTFNCSEARNHIGYSPIVSLQEGVTVTIESFSHAAEDSVEFRY 826
+ ++ SC+ TF+C+ A +GYSP+VSL+EG+T+TI+SFSH ++ +
Sbjct: 310 KYNHSTWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHLRKELSLASF 369
Query: 825 EDCNELSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLSGRTFVSSAAKLLL 646
+ E SKAD LG GRVAD+LLWRDEKK+FT FL LLFYWF G TF+SSAA+LLL
Sbjct: 370 TEFTEESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFLXRGGTFISSAAQLLL 429
Query: 645 LISVVLFSHGILPASLFGYTVKKISPSHVEVAEKVT-DAIMYLASAWNHCMRTLSLLGRG 469
I +VL+ +G +P +++G+ V+K+S + ++ V D+IM LA WN + L RG
Sbjct: 430 SIIIVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHNARALARG 489
Query: 468 GDWNIFLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYEDKVDRVTEFSSTGI 289
DWN FLK + LY+LKLL + S + + GV LVFAFTAFF+Y QYE ++D +F GI
Sbjct: 490 EDWNYFLKAVAFLYLLKLLLARSLTMLLGVGLVFAFTAFFVYEQYEAEIDEFAKFFFIGI 549
Query: 288 KSLKMLLLRNVPVSVASLL 232
LK LL ++P + S L
Sbjct: 550 MELKKLLASHLPTPLMSFL 568
>ref|XP_003539040.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
3-like [Glycine max]
Length = 575
Score = 570 bits (1470), Expect = e-160
Identities = 294/576 (51%), Positives = 393/576 (68%), Gaps = 1/576 (0%)
Frame = -2
Query: 1944 MAMINEGFENPNNNSKTICVVLGGRSFIGKPLVSMLLKSKKWIVRIADSDPCLNLDLVEK 1765
MA++++ F + N + CVVLGGR F+G+ LV LLK WIVR+ADS L L E
Sbjct: 1 MAVVDDRFADLNPKT---CVVLGGRGFLGRSLVLRLLKLGNWIVRVADSAQSLQLHHSE- 56
Query: 1764 TSILSESISSGIVSYFQLDLHDKSQIIKAVQGSTVVYHTGATGFSSKGFYDQYITTVQGT 1585
S+L +++SS SYF +DL DK I+K ++GS+VV++ G FY Y VQG
Sbjct: 57 -SLLEQALSSSRASYFHVDLLDKRSIVKVLEGSSVVFYMDVAGVDVNNFYTCYKLMVQGA 115
Query: 1584 KNVIRVCHECKVKRLIYTSSADVVFDGIHDVLNGNESLPYPERFGDTLSDLKAQEEALVL 1405
KNVI C EC+V+RLIY SSADVV G+HD+ +G+ESL YP + +TLSDLKAQ EAL+L
Sbjct: 116 KNVISACRECRVRRLIYNSSADVVVGGLHDIRDGDESLAYPWKTNNTLSDLKAQAEALIL 175
Query: 1404 FANCSDGLLTTVLRPSNVFGPGDVKLVPFLLRAAKSGLAKFIVGTGENVCDFTYVDNVAH 1225
AN DGLLT LRPSNVFGPGD + VP+ L+ A+ G +KFI+GTG+N+ DFT+ +NV H
Sbjct: 176 SANDIDGLLTCSLRPSNVFGPGDTEFVPYFLKLARYGFSKFIIGTGDNLSDFTFSENVTH 235
Query: 1224 AHVCAEEALRSRTAFVAGKAFFITNLDPMKFWDFVSLIFKGLGYRRPRFKVPAKLFLFVA 1045
AH+CAEEAL +T VAGK FFITNL+PMKFW+F+SL+ +GL Y+RP K+PAKL ++
Sbjct: 236 AHICAEEALNFQTVSVAGKTFFITNLEPMKFWEFLSLLLEGLEYQRPFIKLPAKLVQYIL 295
Query: 1044 VLSNWLLEKLEQRDAHSVLTPAIVRQLSCSMTFNCSEARNHIGYSPIVSLQEGVTVTIES 865
+ W+ EKL R L + S + TFNC A+ IGYSPIVSL+EGVT+TIES
Sbjct: 296 SVLKWVHEKLGSRYFSYPLLVHFFQLASYTRTFNCMAAQKDIGYSPIVSLEEGVTLTIES 355
Query: 864 FSHAAEDSVEFRYEDCNELSKADIFLGHGRVADILLWRDEKKTFTYFLGLVLLFYWFFLS 685
FSH A DS R E SKAD LG G+VADILLWR+EKK+F FL LVLLFYWFFLS
Sbjct: 356 FSHLARDSSFSRCCSSTEQSKADKLLGGGKVADILLWREEKKSFACFLVLVLLFYWFFLS 415
Query: 684 GRTFVSSAAKLLLLISVVLFSHGILPASLFGYTVKKISPSHVEVAE-KVTDAIMYLASAW 508
GRTF+S+AA+LLL +++L+ HG LP+ LFG+ +++I ++ E+++ V D++ W
Sbjct: 416 GRTFISAAARLLLFSTLLLYGHGFLPSKLFGFHIQRIPTTYFEISDTAVKDSVTTTVYLW 475
Query: 507 NHCMRTLSLLGRGGDWNIFLKVMCTLYMLKLLFSLSFSAVTGVALVFAFTAFFIYMQYED 328
N + +S L +G DW+ F K+ LY+LKL+ S F+ + G+ L AF AFF+Y QYE
Sbjct: 476 NRGFQNISGLAQGDDWSAFFKIAVFLYLLKLILSELFTKMIGIGLAVAFMAFFVYEQYES 535
Query: 327 KVDRVTEFSSTGIKSLKMLLLRNVPVSVASLLDKSE 220
++D + +F T +K + L+R PVS++ LL E
Sbjct: 536 EIDGLVDFLFTSLKEFMIHLMRISPVSISRLLHYQE 571