BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00011915
         (890 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAN03619.1|AF406816_1 sphingolipid long chain base delta 8 de...   444   e-122
gb|AAQ10732.1| delta-8-sphingolipid desaturase [Anemone leveillei]    425   e-117
gb|AAQ10731.1| delta-6-fatty acid desaturase [Anemone leveillei]      381   e-103
gb|ABP01349.1| D8-sphingolipid desaturase [Jatropha curcas]           374   e-101
gb|ABO31111.1| sphingolipid delta-8 desaturase [Nicotiana tabacum]    369   e-100

>gb|AAN03619.1|AF406816_1 sphingolipid long chain base delta 8 desaturase [Aquilegia
           vulgaris]
          Length = 446

 Score =  444 bits (1142), Expect = e-122
 Identities = 203/254 (79%), Positives = 227/254 (89%)
 Frame = -2

Query: 763 MTEKSRREITSSELKSHDKKGDLWISIQGKVYDVTNWLKDHPGGESPLMNLAGQDVTDAF 584
           MTEK RREITS ELK H+K GD+WISIQGK+YDV+ W+KDHPGGE+PL+NLAGQDVTDAF
Sbjct: 1   MTEK-RREITSEELKKHNKHGDIWISIQGKIYDVSEWIKDHPGGEAPLLNLAGQDVTDAF 59

Query: 583 VAFHPGSAWKYLDKFFIGYLQDYTVSEVSKDYRKLVTQFSKAGLFEKKGHWICISLVTSA 404
           VAFHPGSAWKYLDKFFIGYL+DYT+SEVSKDYRKLV +FSKAGL++KKGH I  SL    
Sbjct: 60  VAFHPGSAWKYLDKFFIGYLKDYTISEVSKDYRKLVAEFSKAGLYDKKGHHILFSLTFVT 119

Query: 403 FLFTLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTG 224
            L  +SV+GVL SDK W H+ SA ++GLLWMQIGFVGHDSGHYNIM+TPKLNRFMQIFTG
Sbjct: 120 ILMAISVMGVLCSDKTWAHLASAAVLGLLWMQIGFVGHDSGHYNIMLTPKLNRFMQIFTG 179

Query: 223 NCVTGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDS 44
           NC+TGISIGWWKW H AHHIAVNSLD+DPDLQHIPFLAVSSDIF SL S FYGRKMTFD 
Sbjct: 180 NCITGISIGWWKWNHNAHHIAVNSLDYDPDLQHIPFLAVSSDIFSSLTSKFYGRKMTFDP 239

Query: 43  VARFLVSYQHWSFY 2
           +ARFL+S+QHW+FY
Sbjct: 240 IARFLISFQHWTFY 253


>gb|AAQ10732.1| delta-8-sphingolipid desaturase [Anemone leveillei]
          Length = 446

 Score =  425 bits (1092), Expect = e-117
 Identities = 197/254 (77%), Positives = 220/254 (86%)
 Frame = -2

Query: 763 MTEKSRREITSSELKSHDKKGDLWISIQGKVYDVTNWLKDHPGGESPLMNLAGQDVTDAF 584
           M EK RREI+S EL  H  KGDLWISIQGKVYDV+ W+KDHPGG++PLMNLAGQDVTDAF
Sbjct: 1   MAEK-RREISSQELAQHTNKGDLWISIQGKVYDVSEWVKDHPGGDAPLMNLAGQDVTDAF 59

Query: 583 VAFHPGSAWKYLDKFFIGYLQDYTVSEVSKDYRKLVTQFSKAGLFEKKGHWICISLVTSA 404
           VAFHPGSAWKYLDKF IGYL DY VS+VSKDYRKL ++FSKAGLFEKKGH I +SL++  
Sbjct: 60  VAFHPGSAWKYLDKFHIGYLSDYVVSDVSKDYRKLASEFSKAGLFEKKGHGIFMSLLSVG 119

Query: 403 FLFTLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTG 224
            +F + + GVL S   WVH   A L+G+LWMQIGFVGHDSGHYN+M+TPKLNRF+QIFTG
Sbjct: 120 IMFVVILSGVLLSGNTWVHAACAGLLGILWMQIGFVGHDSGHYNVMLTPKLNRFVQIFTG 179

Query: 223 NCVTGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDS 44
           NCVTGISIGWW+WTHTAHHIAVNSLD+DPDLQH+PFLAVS DI  SL S FYGRKMTFD+
Sbjct: 180 NCVTGISIGWWRWTHTAHHIAVNSLDYDPDLQHVPFLAVSKDILSSLTSKFYGRKMTFDA 239

Query: 43  VARFLVSYQHWSFY 2
            ARFLVSYQHWSFY
Sbjct: 240 AARFLVSYQHWSFY 253


>gb|AAQ10731.1| delta-6-fatty acid desaturase [Anemone leveillei]
          Length = 446

 Score =  381 bits (978), Expect = e-103
 Identities = 178/254 (70%), Positives = 214/254 (84%)
 Frame = -2

Query: 763 MTEKSRREITSSELKSHDKKGDLWISIQGKVYDVTNWLKDHPGGESPLMNLAGQDVTDAF 584
           M EK RR I+S +L+SH+K GD+WISIQGK+YDVT W KDHPGGE PL+NLAGQDVTDAF
Sbjct: 1   MAEK-RRSISSDDLRSHNKPGDVWISIQGKIYDVTEWGKDHPGGEGPLLNLAGQDVTDAF 59

Query: 583 VAFHPGSAWKYLDKFFIGYLQDYTVSEVSKDYRKLVTQFSKAGLFEKKGHWICISLVTSA 404
           VAFHPGSAWK LDKF IGYLQDY VS+VSKDYRKLV++FSKAGL+EKKGH   I L+  +
Sbjct: 60  VAFHPGSAWKNLDKFHIGYLQDYVVSDVSKDYRKLVSEFSKAGLYEKKGHGHLIRLLVMS 119

Query: 403 FLFTLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTG 224
            +F  SV GV+ SDK  VH+ SA L+ ++WMQ GF+GHDSGHYNIM +P+LNR+MQIF+ 
Sbjct: 120 LVFIASVSGVVLSDKTSVHVGSAVLLAVIWMQFGFIGHDSGHYNIMTSPELNRYMQIFSV 179

Query: 223 NCVTGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDS 44
           N V+G+S+GWWK  H AHHIAVNSL++DPDLQ++PFL VS+ IF SL S FY +KMTFD+
Sbjct: 180 NVVSGVSVGWWKRYHNAHHIAVNSLEYDPDLQYVPFLVVSTAIFDSLTSHFYRKKMTFDA 239

Query: 43  VARFLVSYQHWSFY 2
           VARFLVS+QHW+FY
Sbjct: 240 VARFLVSFQHWTFY 253


>gb|ABP01349.1| D8-sphingolipid desaturase [Jatropha curcas]
          Length = 447

 Score =  374 bits (961), Expect = e-101
 Identities = 167/251 (66%), Positives = 204/251 (81%), Gaps = 1/251 (0%)
 Frame = -2

Query: 751 SRREITSSELKSHDKKGDLWISIQGKVYDVTNWLKDHPGGESPLMNLAGQDVTDAFVAFH 572
           S++ I+  ELK H K GDLWISIQGKVY+VT W KDHPGGE PL++L+GQDVTDAFVA+H
Sbjct: 4   SKKYISEEELKKHTKSGDLWISIQGKVYNVTGWTKDHPGGELPLLHLSGQDVTDAFVAYH 63

Query: 571 PGSAWKYLDKFFIGY-LQDYTVSEVSKDYRKLVTQFSKAGLFEKKGHWICISLVTSAFLF 395
           PG+AW+YLDKFF GY L+D++VSEVSKDYRKL+ +F+K GLFEKKGH   ++L   A L 
Sbjct: 64  PGTAWQYLDKFFTGYYLKDHSVSEVSKDYRKLLAEFTKMGLFEKKGHVTLVTLCVVAMLL 123

Query: 394 TLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTGNCV 215
           + S+ G+L SD  WVH++S  L+G LW+Q G++GHDSGHY +M +PK+NR +QI TGNC+
Sbjct: 124 SFSIYGILCSDSTWVHLLSGGLIGFLWIQSGWIGHDSGHYQVMTSPKINRLVQILTGNCL 183

Query: 214 TGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDSVAR 35
            GISI WWKW H  HHIA NSL+FDPDLQH+PF AVSS +F SL+S FY RKM FDSV R
Sbjct: 184 AGISIAWWKWNHNTHHIACNSLEFDPDLQHMPFFAVSSKLFSSLKSYFYERKMNFDSVCR 243

Query: 34  FLVSYQHWSFY 2
           FLVSYQHW+FY
Sbjct: 244 FLVSYQHWTFY 254


>gb|ABO31111.1| sphingolipid delta-8 desaturase [Nicotiana tabacum]
          Length = 447

 Score =  369 bits (946), Expect = e-100
 Identities = 167/251 (66%), Positives = 205/251 (81%), Gaps = 1/251 (0%)
 Frame = -2

Query: 751 SRREITSSELKSHDKKGDLWISIQGKVYDVTNWLKDHPGGESPLMNLAGQDVTDAFVAFH 572
           SR+ ITS ELK+H+K GDLWISIQGKVYDV++W+K+HPGG+ PL+NLAGQDVTDAFVA+H
Sbjct: 4   SRKYITSEELKNHNKPGDLWISIQGKVYDVSDWIKEHPGGDFPLLNLAGQDVTDAFVAYH 63

Query: 571 PGSAWKYLDKFFIG-YLQDYTVSEVSKDYRKLVTQFSKAGLFEKKGHWICISLVTSAFLF 395
           P +AW+YLDKFF G YL+DY+ SEVSKDYR+LV++F+K GLFEKKGH    ++ + A LF
Sbjct: 64  PATAWQYLDKFFNGFYLKDYSASEVSKDYRRLVSEFTKLGLFEKKGHVCFYTMCSIAMLF 123

Query: 394 TLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTGNCV 215
            +SV G++ S+ + VH++S  LMG LW+Q G++GHDSGHY +M T  LNRF Q+ TGNC+
Sbjct: 124 AVSVYGIMCSESVLVHLMSGGLMGFLWIQSGWIGHDSGHYQVMSTRGLNRFAQVLTGNCL 183

Query: 214 TGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDSVAR 35
            GISI WWKW H AHHIAVNSL++DPDLQH+PF  VSS  F SL S FY RKMTFDS  R
Sbjct: 184 AGISIAWWKWNHNAHHIAVNSLEYDPDLQHMPFFVVSSKFFNSLTSYFYDRKMTFDSFTR 243

Query: 34  FLVSYQHWSFY 2
           FLVS QHW+FY
Sbjct: 244 FLVSNQHWTFY 254