BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00011790
         (1259 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    330   6e-88
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           327   4e-87
gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver...   312   1e-82
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]       307   4e-81
ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   305   2e-80

>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  330 bits (845), Expect = 6e-88
 Identities = 163/355 (45%), Positives = 232/355 (65%)
 Frame = -3

Query: 1068 SSTTNYKRRNVAPVAGGAKPILGHLQMLMGGELPHHVLGKLADKYGPAFMIQIGPHPELV 889
            S T   K+   AP+A GA P+LGHL +   GELPH +L  +ADKYG AF ++ G H  LV
Sbjct: 29   SRTLKNKKIRTAPMATGAWPVLGHLHLFGSGELPHKMLAAMADKYGSAFRMKFGKHTTLV 88

Query: 888  VSSWELAKECFTTNDKYFTNRPSNMAMKLLTYDQASVGFAPYGPLWVEMRKVSKSNLLTS 709
            VS   + KECFTTND  F+NRPS  A +L+TYD  SV F PYG  W E+RK+S   LL++
Sbjct: 89   VSDTRIVKECFTTNDTLFSNRPSTKAFQLMTYDNESVAFTPYGSYWREIRKISTLKLLSN 148

Query: 708  QRIQMQKQVRASEVGAFFNELYQLCSTSNDTTCTVTDGAIGHEVGQGSAMVEMNKWFEEL 529
             R+Q  K VRASEV   F  LY  C   +               G    +++M KWFEE+
Sbjct: 149  HRLQAIKDVRASEVNVCFKTLYDQCKNPS---------------GSAPILIDMKKWFEEV 193

Query: 528  TLNVVTRMVSGKQNYGTKARRGDDEAKHFKKVIDDAAQFMGNLVISDIFPSLGWLDYMLG 349
            + NVV R++ G+QN+G+K  +G++EA H+KKV+D+  +     + SD  P LG++D   G
Sbjct: 194  SNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFAPLLGFVDIFQG 253

Query: 348  HVGAMKKTAKELDSIFGSWVDEHQQKRKRVSGNTENGEQDFIDLTLSMMEEIQLHGTDPE 169
            ++ AMK+ AK++D+I  +W++EH++K+  V+      +QDF+D+ LS++EE +L G D +
Sbjct: 254  NLSAMKRNAKKVDAILENWLEEHRKKKNSVA----ESQQDFMDVMLSIVEESKLSGHDAD 309

Query: 168  TFIKSICVGVIFGGGDSTSVALTWVLSILMNNRSALKKAREEIDRQVGTDRKVND 4
              IK+ C+ +I GG D+T+V+LTW++S+LMNNR ALKKAREE+D  VG DR+V D
Sbjct: 310  AVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKAREELDALVGKDRQVED 364


>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  327 bits (838), Expect = 4e-87
 Identities = 164/361 (45%), Positives = 232/361 (64%), Gaps = 4/361 (1%)
 Frame = -3

Query: 1074 SRSSTTNYKRRNVA---PVAGGAKPILGHLQMLMGGELPHHVLGKLADKYGPAFMIQIGP 904
            S S TT+ K + +    P   GA P+ GHL +   GE PH +L KLA+KYGP+F ++ G 
Sbjct: 38   SSSKTTSLKGKKIITRPPAVTGAWPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGK 97

Query: 903  HPELVVSSWELAKECFTTNDKYFTNRPSNMAMKLLTYDQASVGFAPYGPLWVEMRKVSKS 724
            H  LVVS   + KECFTTND  F+NRPS +A  L+TY   S+ F PY P W E+RK+S  
Sbjct: 98   HTTLVVSDTRVVKECFTTNDTLFSNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTL 157

Query: 723  NLLTSQRIQMQKQVRASEVGAFFNELYQLCSTSNDTTCTVTDGAIGHEVGQGSAMVEMNK 544
             LL++ R++  KQ+R SEV   F ELY L +  ND    V               +++ +
Sbjct: 158  KLLSNNRLESIKQLRTSEVSVCFKELYDLTNKKNDNGAPVP--------------IDLKR 203

Query: 543  WFEELTLNVVTRMVSGKQNYGTKARRGDD-EAKHFKKVIDDAAQFMGNLVISDIFPSLGW 367
            WF+E++ NV+ R++ GKQN+G+K   G+D EA H+KK++D+ ++     ++SD+ P LGW
Sbjct: 204  WFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHYKKIMDELSRLSSLTMLSDMVPLLGW 263

Query: 366  LDYMLGHVGAMKKTAKELDSIFGSWVDEHQQKRKRVSGNTENGEQDFIDLTLSMMEEIQL 187
            LDY  G + AMK+  KEL+SI   W++EH+ K+      + +  QDF+D+ LS+ ++ QL
Sbjct: 264  LDYFKGDLRAMKRNGKELNSILQKWLEEHKSKK------SSDARQDFMDVMLSISKDTQL 317

Query: 186  HGTDPETFIKSICVGVIFGGGDSTSVALTWVLSILMNNRSALKKAREEIDRQVGTDRKVN 7
            +G D +TFIK+ C+ +I GG +ST VALTW+LS+LMNNR AL KAREEID  VG DR+V 
Sbjct: 318  YGHDQDTFIKATCLAMIMGGTNSTEVALTWILSLLMNNRCALHKAREEIDLLVGKDRQVE 377

Query: 6    D 4
            D
Sbjct: 378  D 378


>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
          Length = 526

 Score =  312 bits (799), Expect = 1e-82
 Identities = 162/355 (45%), Positives = 222/355 (62%), Gaps = 4/355 (1%)
 Frame = -3

Query: 1056 NYKRRN----VAPVAGGAKPILGHLQMLMGGELPHHVLGKLADKYGPAFMIQIGPHPELV 889
            NY RRN    +AP A G +PI+GHL +   GEL H  LG +AD YGP F I+ G H  LV
Sbjct: 34   NYGRRNPTKKLAPEASGGRPIMGHLHLFNDGELTHRKLGAMADTYGPVFNIRFGSHKTLV 93

Query: 888  VSSWELAKECFTTNDKYFTNRPSNMAMKLLTYDQASVGFAPYGPLWVEMRKVSKSNLLTS 709
            VS WE+ KECFTTNDK F+NRP  + +KL+ YD  SVG+APYG  W ++RK+S   LL++
Sbjct: 94   VSDWEIVKECFTTNDKLFSNRPGTLGIKLMFYDADSVGYAPYGAYWRDLRKISTLKLLSN 153

Query: 708  QRIQMQKQVRASEVGAFFNELYQLCSTSNDTTCTVTDGAIGHEVGQGSAMVEMNKWFEEL 529
             RI   K +R+SEV + F  LY             +    G + G+  A V M+ W  +L
Sbjct: 154  HRIDTIKHLRSSEVESCFESLY-------------SQWGNGEKSGE-FAPVRMDSWLGDL 199

Query: 528  TLNVVTRMVSGKQNYGTKARRGDDEAKHFKKVIDDAAQFMGNLVISDIFPSLGWLDYMLG 349
            T NVV R+V+GK+N+   +  GD  A+ +K  +D+A + M     SD+ PSL WLD + G
Sbjct: 200  TFNVVARIVAGKKNF---SANGDVGAQRYKAAMDEAMRLMRFFAFSDVIPSLSWLDNLRG 256

Query: 348  HVGAMKKTAKELDSIFGSWVDEHQQKRKRVSGNTENGEQDFIDLTLSMMEEIQLHGTDPE 169
             V  MKK A E+DSI  +WV+EH+ KR   SG     E DFID+ L +ME   L G DP+
Sbjct: 257  LVREMKKCASEIDSIMATWVEEHRVKRN--SGGNSQLEHDFIDVCLDIMEHSSLPGDDPD 314

Query: 168  TFIKSICVGVIFGGGDSTSVALTWVLSILMNNRSALKKAREEIDRQVGTDRKVND 4
              +KS C+ +I GG D+T+V LTW +S+L+N+   L+KA+EE++ QVG +R+V+D
Sbjct: 315  LVVKSTCLDMILGGSDTTTVTLTWAMSLLLNHPQVLQKAKEELETQVGKNRQVDD 369


>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 511

 Score =  307 bits (786), Expect = 4e-81
 Identities = 159/355 (44%), Positives = 232/355 (65%)
 Frame = -3

Query: 1068 SSTTNYKRRNVAPVAGGAKPILGHLQMLMGGELPHHVLGKLADKYGPAFMIQIGPHPELV 889
            SS TN  +   AP A GA PI+GHL +L G EL H  LG +ADKYGP F I+IG +  LV
Sbjct: 16   SSKTNTCKE--APEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPIFKIRIGVNHALV 73

Query: 888  VSSWELAKECFTTNDKYFTNRPSNMAMKLLTYDQASVGFAPYGPLWVEMRKVSKSNLLTS 709
            VS+ ++AKECFTTNDK F +RP++ A K+L YD    G APYG  WVE+RK++ S LL++
Sbjct: 74   VSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVELRKITMSELLSN 133

Query: 708  QRIQMQKQVRASEVGAFFNELYQLCSTSNDTTCTVTDGAIGHEVGQGSAMVEMNKWFEEL 529
            +R+++ K VR SE+ A   +LY++                 H+  +G  +V+M +WF +L
Sbjct: 134  RRLELLKHVRDSEIDASIQDLYKVWK--------------NHDKAKGPVLVDMKQWFGDL 179

Query: 528  TLNVVTRMVSGKQNYGTKARRGDDEAKHFKKVIDDAAQFMGNLVISDIFPSLGWLDYMLG 349
            TLNV+ RM++GK+  G+ +   + EA+  +K + D  + +G  +I D  P L W D + G
Sbjct: 180  TLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWFD-LQG 238

Query: 348  HVGAMKKTAKELDSIFGSWVDEHQQKRKRVSGNTENGEQDFIDLTLSMMEEIQLHGTDPE 169
            +   MK TAKELDS+F  W++EH   R R +G   N EQDF+D+ +S++E+ ++   D +
Sbjct: 239  YKKEMKNTAKELDSVFQRWLEEH--NRMRQTGEL-NREQDFMDVLMSILEDTRISEYDND 295

Query: 168  TFIKSICVGVIFGGGDSTSVALTWVLSILMNNRSALKKAREEIDRQVGTDRKVND 4
            T IKS C+ ++ GGGD+T V LTW+LS+L+NN+ ALKKA++E+D  VG DR+V +
Sbjct: 296  TIIKSTCLSIVTGGGDTTMVTLTWILSLLLNNKHALKKAQDELDSHVGKDRQVEE 350


>ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
            gi|147794787|emb|CAN66846.1| hypothetical protein
            VITISV_002367 [Vitis vinifera]
          Length = 528

 Score =  305 bits (780), Expect = 2e-80
 Identities = 158/348 (45%), Positives = 219/348 (62%)
 Frame = -3

Query: 1047 RRNVAPVAGGAKPILGHLQMLMGGELPHHVLGKLADKYGPAFMIQIGPHPELVVSSWELA 868
            +R  AP AGGA PI+GHL +    +L H  LG +ADKYGP F I++G +  LV+SS E+A
Sbjct: 36   KRCTAPRAGGAWPIIGHLHLFGAQQLTHKTLGAMADKYGPVFTIRLGLNEILVLSSSEMA 95

Query: 867  KECFTTNDKYFTNRPSNMAMKLLTYDQASVGFAPYGPLWVEMRKVSKSNLLTSQRIQMQK 688
            +ECFTT+D+ F+ RPS  A K+L YD A  GFAPYG  W EMRK+    LL++ R+ M K
Sbjct: 96   RECFTTHDRVFSTRPSVTASKILGYDFAMFGFAPYGSYWREMRKIVTIELLSNHRLDMLK 155

Query: 687  QVRASEVGAFFNELYQLCSTSNDTTCTVTDGAIGHEVGQGSAMVEMNKWFEELTLNVVTR 508
             +RASEVG    ELY++  +   T               G   V+M +WF +LTLN+  R
Sbjct: 156  HIRASEVGTSIRELYEMWVSERGT--------------DGRVFVDMKRWFGDLTLNLAVR 201

Query: 507  MVSGKQNYGTKARRGDDEAKHFKKVIDDAAQFMGNLVISDIFPSLGWLDYMLGHVGAMKK 328
            +V GK+ +G  A   + E +  +KVI D A   G  V+SD  P L WLD + G+  AMK+
Sbjct: 202  LVGGKRYFGAGADTKEGEGRTCQKVIRDFAHLFGVFVLSDAIPFLSWLD-LKGYKKAMKR 260

Query: 327  TAKELDSIFGSWVDEHQQKRKRVSGNTENGEQDFIDLTLSMMEEIQLHGTDPETFIKSIC 148
            TAKELDS+FG W+ EH  K KR+ G     +QDF+D+ L+++E++   G D +T  K+ C
Sbjct: 261  TAKELDSLFGGWLQEH--KEKRLLGGEGKDDQDFMDVMLTVLEDVNFSGFDADTVNKATC 318

Query: 147  VGVIFGGGDSTSVALTWVLSILMNNRSALKKAREEIDRQVGTDRKVND 4
            + +I  G D+T V LTW LS+L+N+   LKKA+ E+D QVG DR+V++
Sbjct: 319  LNLILAGSDTTKVTLTWALSLLLNHPHVLKKAQAELDIQVGKDRQVDE 366