BLASTX nr result
ID: Coptis21_contig00009588
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00009588
(1012 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002524161.1| pseudouridylate synthase, putative [Ricinus ... 371 e-100
ref|XP_004166389.1| PREDICTED: tRNA pseudouridine synthase A-lik... 370 e-100
ref|XP_004137363.1| PREDICTED: tRNA pseudouridine synthase A-lik... 370 e-100
ref|XP_002331405.1| predicted protein [Populus trichocarpa] gi|2... 365 9e-99
ref|XP_002275354.2| PREDICTED: tRNA pseudouridine synthase A-lik... 360 4e-97
>ref|XP_002524161.1| pseudouridylate synthase, putative [Ricinus communis]
gi|223536579|gb|EEF38224.1| pseudouridylate synthase,
putative [Ricinus communis]
Length = 443
Score = 371 bits (952), Expect = e-100
Identities = 195/349 (55%), Positives = 232/349 (66%), Gaps = 14/349 (4%)
Frame = -1
Query: 1006 EKQTEKKVYLHYNHTDSCNSSRWNARECHEFMYSSRPWQKVVDFYSNVVNGKVSL----S 839
E +T+ K+Y HYNHTDSC S RW ARE +FMY+ RPWQ+V DFYSNVVNG++S
Sbjct: 26 ETKTQNKIYFHYNHTDSCKSFRWTARESFQFMYA-RPWQEVSDFYSNVVNGRLSFLELFR 84
Query: 838 SSILFQNEDPKTCINEVVEETKGRTT----------GRWERVTFKIILSYHGTSFDGWQK 689
S + F +D K ++E +T GRW RV FKI LSYHG SFDGWQK
Sbjct: 85 SQMHFVRDDAK------IQEDSNKTELEHFSNEDRFGRWARVNFKIDLSYHGGSFDGWQK 138
Query: 688 QPGLNTVQGIVERSLGRFVDDKKAEKLKDKCLPLEGCTTVAGRTDKGVTALKQACSFYTW 509
QPGLNTVQG+VE+SLG+FVD+KKA++LK+ C PLEGC VAGRTDKGV+AL+Q CSFYTW
Sbjct: 139 QPGLNTVQGLVEKSLGKFVDEKKAQQLKEACKPLEGCAVVAGRTDKGVSALRQVCSFYTW 198
Query: 508 RKDIRVQDVEDAINEAAPGKLRVISVSKVSREFHPNFSAKWRRYFYIFPLNDXXXXXXXX 329
RKD+R ++EDAIN +APGK+RVISVS+VSR FHPNFSAKWRRY YIFP+ND
Sbjct: 199 RKDVRPHEIEDAINSSAPGKIRVISVSEVSRVFHPNFSAKWRRYLYIFPINDGEDSEQSF 258
Query: 328 XXXXXENYGIEEHNRKLINGYGAHFXXXXXXXXXXXXXXLARDDLAESERGRKPVSFSID 149
EN E + NG L D E E +KP FSI
Sbjct: 259 DSEDLENLRSYEKYDEQRNG-------CAELTSEEHVEELITSDKDELEGAKKPRIFSIC 311
Query: 148 KVNXXXXXXQGRSLSYKMFARDTKASRSMGPPTECIIYHVRATEATLPC 2
+VN +G+ LSYK+FARDTKASR++GPPTEC IYH RATEA LPC
Sbjct: 312 RVNQLLQQLEGKLLSYKIFARDTKASRNVGPPTECFIYHARATEARLPC 360
>ref|XP_004166389.1| PREDICTED: tRNA pseudouridine synthase A-like [Cucumis sativus]
Length = 444
Score = 370 bits (950), Expect = e-100
Identities = 192/345 (55%), Positives = 231/345 (66%), Gaps = 8/345 (2%)
Frame = -1
Query: 1012 MMEKQTEKKVYLHYNHTDSCNSSRWNARECHEFMYSSRPWQKVVDFYSNVVNGKVSLSS- 836
+ME + ++K YLHYNHTD C S+RW ARE ++FMY RPWQ+V+DFYS+ VNG+ SLSS
Sbjct: 22 LMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYG-RPWQEVLDFYSHAVNGRFSLSSL 80
Query: 835 ---SILFQNEDPKTCI----NEVVEETKGRTTGRWERVTFKIILSYHGTSFDGWQKQPGL 677
I ++D +T + E+ TGRW R TFKI+LSY G SFDGWQKQPGL
Sbjct: 81 LGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARATFKIVLSYDGNSFDGWQKQPGL 140
Query: 676 NTVQGIVERSLGRFVDDKKAEKLKDKCLPLEGCTTVAGRTDKGVTALKQACSFYTWRKDI 497
NTVQG+VERSLG+FVD+KKA+ LKDKCLPLEGC VAGRTDKGVTAL+Q CSFYTWR D+
Sbjct: 141 NTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCALVAGRTDKGVTALRQVCSFYTWRTDV 200
Query: 496 RVQDVEDAINEAAPGKLRVISVSKVSREFHPNFSAKWRRYFYIFPLNDXXXXXXXXXXXX 317
+ QD+ED+IN AAPGKLR SVS+VSR FHPNFSAKWRRY YI PLND
Sbjct: 201 KPQDIEDSINSAAPGKLRTSSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSTTWE 260
Query: 316 XENYGIEEHNRKLINGYGAHFXXXXXXXXXXXXXXLARDDLAESERGRKPVSFSIDKVNX 137
EE+ K+ G ++L ++ KP +FSI +VN
Sbjct: 261 NVENREEENFVKIEASNGEKCVDGIHDNLVLSPND---EELVSAD---KPQTFSITRVNQ 314
Query: 136 XXXXXQGRSLSYKMFARDTKASRSMGPPTECIIYHVRATEATLPC 2
+G+ LSYKMFARDTKASR+ GPPTEC +YH RA E LPC
Sbjct: 315 LLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRLPC 359
>ref|XP_004137363.1| PREDICTED: tRNA pseudouridine synthase A-like [Cucumis sativus]
Length = 444
Score = 370 bits (949), Expect = e-100
Identities = 194/351 (55%), Positives = 231/351 (65%), Gaps = 14/351 (3%)
Frame = -1
Query: 1012 MMEKQTEKKVYLHYNHTDSCNSSRWNARECHEFMYSSRPWQKVVDFYSNVVNGKVSLSS- 836
+ME + ++K YLHYNHTD C S+RW ARE ++FMY RPWQ+V+DFYS+ VNG+ SLSS
Sbjct: 22 LMENRAQRKDYLHYNHTDRCRSARWTARESYQFMYG-RPWQEVLDFYSHAVNGRFSLSSL 80
Query: 835 ---SILFQNEDPKTCI----NEVVEETKGRTTGRWERVTFKIILSYHGTSFDGWQKQPGL 677
I ++D +T + E+ TGRW R TFKI+LSY G SFDGWQKQPGL
Sbjct: 81 LGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARATFKIVLSYDGNSFDGWQKQPGL 140
Query: 676 NTVQGIVERSLGRFVDDKKAEKLKDKCLPLEGCTTVAGRTDKGVTALKQACSFYTWRKDI 497
NTVQG+VERSLG+FVD+KKA+ LKDKCLPLEGC VAGRTDKGVTAL+Q CSFYTWR D+
Sbjct: 141 NTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCALVAGRTDKGVTALRQVCSFYTWRTDV 200
Query: 496 RVQDVEDAINEAAPGKLRVISVSKVSREFHPNFSAKWRRYFYIFPLNDXXXXXXXXXXXX 317
+ QD+ED+IN AAPGKLR SVS+VSR FHPNFSAKWRRY YI PLND
Sbjct: 201 KPQDIEDSINSAAPGKLRTSSVSQVSRIFHPNFSAKWRRYLYILPLNDGENMEPDSTTWE 260
Query: 316 XENYGIEEHNRKLINGYGAHFXXXXXXXXXXXXXXLARDDLAESERGR------KPVSFS 155
EE+ K+ G D+L S + KP +FS
Sbjct: 261 NVENREEENFVKIEASNG------------EKCVDGIHDNLVLSPNDQELVSADKPQTFS 308
Query: 154 IDKVNXXXXXXQGRSLSYKMFARDTKASRSMGPPTECIIYHVRATEATLPC 2
I +VN +G+ LSYKMFARDTKASR+ GPPTEC +YH RA E LPC
Sbjct: 309 ITRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRLPC 359
>ref|XP_002331405.1| predicted protein [Populus trichocarpa] gi|222873619|gb|EEF10750.1|
predicted protein [Populus trichocarpa]
Length = 428
Score = 365 bits (937), Expect = 9e-99
Identities = 192/353 (54%), Positives = 236/353 (66%), Gaps = 18/353 (5%)
Frame = -1
Query: 1009 MEKQTEKKVYLHYNHTDSCNSSRWNARECHEFMYSSRPWQKVVDFYSNVVNGKVSL---- 842
ME Q +K VY+ YNHTDSC SRW ARE +FM++ RPWQ+VVDFYS VNG++SL
Sbjct: 1 MEAQNDK-VYVQYNHTDSCKFSRWTARESFQFMHA-RPWQEVVDFYSKSVNGQLSLLELF 58
Query: 841 SSSILFQNEDPKTC---INEVVEETKGRT-----------TGRWERVTFKIILSYHGTSF 704
+ + F E K ++ +EE T +GRW RVTFKI+LSYHG SF
Sbjct: 59 GTQVFFTMELKKAHDVHCDDKIEEVSNETGLLEGVSNVDKSGRWARVTFKIVLSYHGGSF 118
Query: 703 DGWQKQPGLNTVQGIVERSLGRFVDDKKAEKLKDKCLPLEGCTTVAGRTDKGVTALKQAC 524
DGWQKQPGLNTVQG+VE+SLGRFVD+KKA++LK++C PLEGC +VAGRTDKGV+AL+Q C
Sbjct: 119 DGWQKQPGLNTVQGLVEKSLGRFVDEKKAQQLKEQCKPLEGCASVAGRTDKGVSALQQVC 178
Query: 523 SFYTWRKDIRVQDVEDAINEAAPGKLRVISVSKVSREFHPNFSAKWRRYFYIFPLNDXXX 344
SFYTWRKD++ ++EDAIN+ APGK+RV S+S+VSR FHPNFSAKWRRY YIFPLND
Sbjct: 179 SFYTWRKDVKPHEIEDAINDVAPGKVRVESISEVSRAFHPNFSAKWRRYLYIFPLNDGEN 238
Query: 343 XXXXXXXXXXENYGIEEHNRKLINGYGAHFXXXXXXXXXXXXXXLARDDLAESERGRKPV 164
EN+ E+ K N G D E + +KP
Sbjct: 239 REEIEGEGGIENFSSHENCEKQRNECG-------ELASEENVENSIISDEDELQGAKKPR 291
Query: 163 SFSIDKVNXXXXXXQGRSLSYKMFARDTKASRSMGPPTECIIYHVRATEATLP 5
SFS+ +VN +G+ LSYKMFARDTKASR++GPPTEC +YH RATE LP
Sbjct: 292 SFSVCRVNQLLQQLEGKLLSYKMFARDTKASRNVGPPTECFLYHARATETRLP 344
>ref|XP_002275354.2| PREDICTED: tRNA pseudouridine synthase A-like [Vitis vinifera]
Length = 433
Score = 360 bits (923), Expect = 4e-97
Identities = 190/346 (54%), Positives = 230/346 (66%), Gaps = 11/346 (3%)
Frame = -1
Query: 1012 MMEKQTEKKVYLHYNHTDSCNSSRWNARECHEFMYSSRPWQKVVDFYSNVVNGKVSLSSS 833
+ME + E KVY+HY HT SRW ARE H+FMY RPWQ+V+DFYSNVV G+ SL
Sbjct: 13 LMENEKENKVYVHYTHTHPTKFSRWTARESHQFMYK-RPWQQVLDFYSNVVTGRCSLLGL 71
Query: 832 ILFQNE----------DPKTCINEVVEETKGRTTGRWERVTFKIILSYHGTSFDGWQKQP 683
+ + KT V+ + +G GRW R+TFKI+LSYHG SFDGWQKQP
Sbjct: 72 LGIETHPIHDGEIVEASDKTEFGNVLTKDRG---GRWARLTFKIVLSYHGASFDGWQKQP 128
Query: 682 GLNTVQGIVERSLGRFVDDKKAEKLKDKCLPLEGCTTVAGRTDKGVTALKQACSFYTWRK 503
GLNTVQG+VE SLGRFVD++KA++LK+K LP+EG TVAGRTDKGVTAL+Q CSFYTW+K
Sbjct: 129 GLNTVQGLVEASLGRFVDERKAQQLKEKNLPVEGMVTVAGRTDKGVTALQQVCSFYTWKK 188
Query: 502 DIRVQDVEDAINEAAPGKLRVISVSKVSREFHPNFSAKWRRYFYIFPLNDXXXXXXXXXX 323
D++VQD+EDAIN AAPGKLRVISVS+VSR FHPNFSAKWR YFYIFPLND
Sbjct: 189 DVKVQDIEDAINGAAPGKLRVISVSEVSRIFHPNFSAKWRHYFYIFPLNDGDDGEQISEN 248
Query: 322 XXXENYGIEEHNRKLINGYGAH-FXXXXXXXXXXXXXXLARDDLAESERGRKPVSFSIDK 146
++ YG L DD E E +KP +FSI +
Sbjct: 249 E------MDIQKISFNEKYGEQGSECPAQYNGGENTENLIFDDDGEFESRKKPSTFSISR 302
Query: 145 VNXXXXXXQGRSLSYKMFARDTKASRSMGPPTECIIYHVRATEATL 8
V+ +G+ LSYK+FARDTKASR++GPPTEC ++H RATEA L
Sbjct: 303 VDQLLRQLEGKLLSYKIFARDTKASRNIGPPTECFVFHARATEARL 348