BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00008935
         (1408 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16435.3| unnamed protein product [Vitis vinifera]              652   0.0  
ref|XP_004142138.1| PREDICTED: DNA topoisomerase 6 subunit B-lik...   622   e-176
ref|XP_002283111.2| PREDICTED: DNA topoisomerase 6 subunit B [Vi...   611   e-172
gb|ADE77939.1| unknown [Picea sitchensis]                             610   e-172
gb|ADE75679.1| unknown [Picea sitchensis]                             608   e-171

>emb|CBI16435.3| unnamed protein product [Vitis vinifera]
          Length = 673

 Score =  652 bits (1683), Expect = 0.0
 Identities = 326/383 (85%), Positives = 353/383 (92%), Gaps = 1/383 (0%)
 Frame = +3

Query: 3    QFLFRFVSDAVDKNVTIRFARRTDVMPPIPVETKYHPSAVDLLLIKRLIAETSKQNLLQF 182
            QFLF+FVSD  DKNVTIRFARRTD+MPP+P ETKYHPSAVDLLLIKRLI+ETSK+NLLQF
Sbjct: 290  QFLFKFVSDTSDKNVTIRFARRTDIMPPVPAETKYHPSAVDLLLIKRLISETSKENLLQF 349

Query: 183  LQHEFINIGKSYAQRLIEEMGPDFSPKTAVKSLTSQQIVRIQQLFRQAKFDDPSGDCLSP 362
            LQHEF+NIGKSYA+RLI EMGPDFSPK AVKSLTSQQIVRI QLFRQAKFDDPSGDCLSP
Sbjct: 350  LQHEFVNIGKSYAERLIGEMGPDFSPKMAVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSP 409

Query: 363  AGEYNLRLGIVKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGKDVKQGLNIFRFANRI 542
            AGEYNLRLGI+KELHPDMVATYSGSAQVFEGHPF+VEAGVS+GGKDVKQGLNIFRFANRI
Sbjct: 410  AGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFMVEAGVSIGGKDVKQGLNIFRFANRI 469

Query: 543  PLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDIKE 722
            PLLFEQGADVVTRTALKRINWNSYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIGDDI E
Sbjct: 470  PLLFEQGADVVTRTALKRINWNSYKINQAQDKIGVFVSIVSTKIPFKGTGKEYIGDDISE 529

Query: 723  IASAVKTSIQQCCVQLKSKIVKKIQAREQQERKRNLSKYIPDVTNAIYGILSDTAQQHA- 899
            IASAVK+SIQQCC+QLKSKIVKKIQAR QQERKRNLSKYIPD T AIY +L + AQ HA 
Sbjct: 530  IASAVKSSIQQCCIQLKSKIVKKIQARVQQERKRNLSKYIPDATGAIYDVLREMAQLHAS 589

Query: 900  KKRRYEGEEAEMLKKVSAKVLTKEMLREELAKHVEQVDYEMALDYAMQSGVSEEPKEAIY 1079
            KK+RYE E+A++L+KVS   +TK+ L E+LAKHVEQVDYEMAL+YA QSGVSEEP+EAIY
Sbjct: 590  KKKRYEDEDADVLRKVSTHSITKQTLSEKLAKHVEQVDYEMALEYATQSGVSEEPREAIY 649

Query: 1080 IQALEGGHNFTDFHSSVCVLRLF 1148
            +Q+LE G NF DFHS V   RL+
Sbjct: 650  LQSLEDGKNFIDFHSPVFAFRLY 672


>ref|XP_004142138.1| PREDICTED: DNA topoisomerase 6 subunit B-like [Cucumis sativus]
            gi|449525918|ref|XP_004169963.1| PREDICTED: DNA
            topoisomerase 6 subunit B-like [Cucumis sativus]
          Length = 696

 Score =  622 bits (1603), Expect = e-176
 Identities = 314/405 (77%), Positives = 353/405 (87%), Gaps = 24/405 (5%)
 Frame = +3

Query: 3    QFLFRFVSDAVDKNVTIRFARRTDVMPPIPVETKYHPSAVDLLLIKRLIAETSKQNLLQF 182
            QFLFRFVSD  DKN+TIRF+RRTDVMPP+P+ETK+HPSAVDLLLIKRLIAET+KQNLLQF
Sbjct: 290  QFLFRFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQF 349

Query: 183  LQHEFINIGKSYAQRLIEEMGPDFSPKTAVKSLTSQQIVRIQQLFRQAKFDDPSGDCLSP 362
            LQHEF+NIGK +A+RLI EMGP+FSPK +VKSLTSQQIVRI QLFRQAKFDDPSGDCLSP
Sbjct: 350  LQHEFVNIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSP 409

Query: 363  AGEYNLRLGIVKELHPDMVATYSG-----------------------SAQVFEGHPFIVE 473
            AGEYNLRLGI+KELHPDMVATYSG                       SAQVFEGHPFIVE
Sbjct: 410  AGEYNLRLGIIKELHPDMVATYSGRLYLNLELCKLSSKYVNLMSNVDSAQVFEGHPFIVE 469

Query: 474  AGVSLGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFV 653
            AGVS+GGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQ QDKIGVFV
Sbjct: 470  AGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQAQDKIGVFV 529

Query: 654  SIVSTKIPFKGTGKEYIGDDIKEIASAVKTSIQQCCVQLKSKIVKKIQAREQQERKRNLS 833
            SIVSTKIPFKGTGKEYIGDDI EIASAVK++IQQCC+QLKSKIVKKIQAREQQERKRNLS
Sbjct: 530  SIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLS 589

Query: 834  KYIPDVTNAIYGILSDTAQQHA-KKRRYEGEEAEMLKKVSAKVLTKEMLREELAKHVEQV 1010
            +YIPD TNA+Y +L + AQ HA KK+R++ E+AE+L KVSA+++T E LR++LA+HVEQV
Sbjct: 590  RYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAQHVEQV 649

Query: 1011 DYEMALDYAMQSGVSEEPKEAIYIQALEGGHNFTDFHSSVCVLRL 1145
            D+EMA++YA QSG S E KE +YIQ+L+  H+FTDF S + V RL
Sbjct: 650  DHEMAMEYATQSGASAEAKEEMYIQSLDATHSFTDFQSPLFVFRL 694


>ref|XP_002283111.2| PREDICTED: DNA topoisomerase 6 subunit B [Vitis vinifera]
          Length = 656

 Score =  611 bits (1576), Expect = e-172
 Identities = 310/383 (80%), Positives = 337/383 (87%), Gaps = 1/383 (0%)
 Frame = +3

Query: 3    QFLFRFVSDAVDKNVTIRFARRTDVMPPIPVETKYHPSAVDLLLIKRLIAETSKQNLLQF 182
            QFLF+FVSD  DKNVTIRFARRTD+MPP+P ETKYHPSAVDLLLIKRLI+ETSK+NLLQF
Sbjct: 290  QFLFKFVSDTSDKNVTIRFARRTDIMPPVPAETKYHPSAVDLLLIKRLISETSKENLLQF 349

Query: 183  LQHEFINIGKSYAQRLIEEMGPDFSPKTAVKSLTSQQIVRIQQLFRQAKFDDPSGDCLSP 362
            LQHEF+NIGKSYA+RLI EMGPDFSPK AVKSLTSQQIVRI QLFRQAKFDDPSGDCLSP
Sbjct: 350  LQHEFVNIGKSYAERLIGEMGPDFSPKMAVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSP 409

Query: 363  AGEYNLRLGIVKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGKDVKQGLNIFRFANRI 542
            AGEYNLRLGI+KELHPDMVATYSGSAQVFEGHPF+VEAGVS+GGKDVKQGLNIFRFANRI
Sbjct: 410  AGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFMVEAGVSIGGKDVKQGLNIFRFANRI 469

Query: 543  PLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDIKE 722
            PLLFEQGADVVTRTALKRINWNSYKINQ QDKIGVFVSIVSTKIPFKGT           
Sbjct: 470  PLLFEQGADVVTRTALKRINWNSYKINQAQDKIGVFVSIVSTKIPFKGT----------- 518

Query: 723  IASAVKTSIQQCCVQLKSKIVKKIQAREQQERKRNLSKYIPDVTNAIYGILSDTAQQHA- 899
                  +SIQQCC+QLKSKIVKKIQAR QQERKRNLSKYIPD T AIY +L + AQ HA 
Sbjct: 519  ------SSIQQCCIQLKSKIVKKIQARVQQERKRNLSKYIPDATGAIYDVLREMAQLHAS 572

Query: 900  KKRRYEGEEAEMLKKVSAKVLTKEMLREELAKHVEQVDYEMALDYAMQSGVSEEPKEAIY 1079
            KK+RYE E+A++L+KVS   +TK+ L E+LAKHVEQVDYEMAL+YA QSGVSEEP+EAIY
Sbjct: 573  KKKRYEDEDADVLRKVSTHSITKQTLSEKLAKHVEQVDYEMALEYATQSGVSEEPREAIY 632

Query: 1080 IQALEGGHNFTDFHSSVCVLRLF 1148
            +Q+LE G NF DFHS V   RL+
Sbjct: 633  LQSLEDGKNFIDFHSPVFAFRLY 655


>gb|ADE77939.1| unknown [Picea sitchensis]
          Length = 472

 Score =  610 bits (1574), Expect = e-172
 Identities = 303/383 (79%), Positives = 341/383 (89%), Gaps = 1/383 (0%)
 Frame = +3

Query: 3    QFLFRFVSDAVDKNVTIRFARRTDVMPPIPVETKYHPSAVDLLLIKRLIAETSKQNLLQF 182
            QFLFR+VS  VDKNVTIRFARRTDVMPP+P+ET YHPSAVDLLLIKRLIAETSKQNL+QF
Sbjct: 89   QFLFRYVSATVDKNVTIRFARRTDVMPPVPIETNYHPSAVDLLLIKRLIAETSKQNLIQF 148

Query: 183  LQHEFINIGKSYAQRLIEEMGPDFSPKTAVKSLTSQQIVRIQQLFRQAKFDDPSGDCLSP 362
            LQHEF+NI KS+A RLI EMGPDF+PK AVKSL+SQQIVRI QLFRQAKFDDPSGDCLSP
Sbjct: 149  LQHEFVNISKSHADRLIGEMGPDFTPKMAVKSLSSQQIVRIHQLFRQAKFDDPSGDCLSP 208

Query: 363  AGEYNLRLGIVKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGKDVKQGLNIFRFANRI 542
            AGEYNLRLGI+KELHPDMVATY GS  VFEGHPFIVEAG+SLGGKDVKQG+N+FRFANRI
Sbjct: 209  AGEYNLRLGIIKELHPDMVATYEGSPHVFEGHPFIVEAGISLGGKDVKQGINVFRFANRI 268

Query: 543  PLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDIKE 722
            PLLFEQGADV+TRTA+KRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDI E
Sbjct: 269  PLLFEQGADVITRTAMKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDITE 328

Query: 723  IASAVKTSIQQCCVQLKSKIVKKIQAREQQERKRNLSKYIPDVTNAIYGILSDTAQ-QHA 899
            IA++VK +IQQCCVQLKSKIV+K QARE+QERKRNL+KYIPD + AIY +L D  Q +  
Sbjct: 329  IATSVKAAIQQCCVQLKSKIVRKQQARERQERKRNLTKYIPDASRAIYEVLKDIVQLRSP 388

Query: 900  KKRRYEGEEAEMLKKVSAKVLTKEMLREELAKHVEQVDYEMALDYAMQSGVSEEPKEAIY 1079
            KK RY     E+L +VS++ +T+  LRE+LA+HVEQVD EMAL+YA QSG+++E +E  Y
Sbjct: 389  KKPRYGDVYEEILDRVSSREITETTLREKLAQHVEQVDIEMALEYATQSGITQEARETTY 448

Query: 1080 IQALEGGHNFTDFHSSVCVLRLF 1148
            I+ALEG  NF DFHS VCV+RLF
Sbjct: 449  IRALEGVQNFYDFHSPVCVIRLF 471


>gb|ADE75679.1| unknown [Picea sitchensis]
          Length = 676

 Score =  608 bits (1568), Expect = e-171
 Identities = 302/383 (78%), Positives = 340/383 (88%), Gaps = 1/383 (0%)
 Frame = +3

Query: 3    QFLFRFVSDAVDKNVTIRFARRTDVMPPIPVETKYHPSAVDLLLIKRLIAETSKQNLLQF 182
            Q LFR+VS  VDKNVTIRFARRTDVMPP+P+ET YHPSAVDLLLIKRLIAETSKQNL+QF
Sbjct: 293  QLLFRYVSATVDKNVTIRFARRTDVMPPVPIETNYHPSAVDLLLIKRLIAETSKQNLIQF 352

Query: 183  LQHEFINIGKSYAQRLIEEMGPDFSPKTAVKSLTSQQIVRIQQLFRQAKFDDPSGDCLSP 362
            LQHEF+NI KS+A RLI EMGPDF+PK AVKSL+SQQIVRI QLFRQAKFDDPSGDCLSP
Sbjct: 353  LQHEFVNISKSHADRLIGEMGPDFTPKMAVKSLSSQQIVRIHQLFRQAKFDDPSGDCLSP 412

Query: 363  AGEYNLRLGIVKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGKDVKQGLNIFRFANRI 542
            AGEYNLRLGI+KELHPDMVATY GS  VFEGHPFIVEAG+SLGGKDVKQG+N+FRFANRI
Sbjct: 413  AGEYNLRLGIIKELHPDMVATYEGSPHVFEGHPFIVEAGISLGGKDVKQGINVFRFANRI 472

Query: 543  PLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDIKE 722
            PLLFEQGADV+TRTA+KRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDI E
Sbjct: 473  PLLFEQGADVITRTAMKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDITE 532

Query: 723  IASAVKTSIQQCCVQLKSKIVKKIQAREQQERKRNLSKYIPDVTNAIYGILSDTAQ-QHA 899
            IA++VK +IQQCCVQLKSKIV+K QARE+QERKRNL+KYIPD + AIY +L D  Q +  
Sbjct: 533  IATSVKAAIQQCCVQLKSKIVRKQQARERQERKRNLTKYIPDASRAIYEVLKDIVQLRSP 592

Query: 900  KKRRYEGEEAEMLKKVSAKVLTKEMLREELAKHVEQVDYEMALDYAMQSGVSEEPKEAIY 1079
            KK RY     E+L +VS++ +T+  LRE+LA+HVEQVD EMAL+YA QSG+++E +E  Y
Sbjct: 593  KKPRYGDVYEEILDRVSSREITETTLREKLAQHVEQVDIEMALEYATQSGITQEARETTY 652

Query: 1080 IQALEGGHNFTDFHSSVCVLRLF 1148
            I+ALEG  NF DFHS VCV+RLF
Sbjct: 653  IRALEGVQNFYDFHSPVCVIRLF 675