BLASTX nr result
ID: Coptis21_contig00008933
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00008933
(1660 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera] 686 0.0
ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinife... 686 0.0
emb|CAN66537.1| hypothetical protein VITISV_029635 [Vitis vinifera] 672 0.0
ref|XP_003554965.1| PREDICTED: cytochrome P450 85A-like [Glycine... 668 0.0
dbj|BAF56235.1| cytochrome P450 enzyme [Pisum sativum] 664 0.0
>gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera]
Length = 460
Score = 686 bits (1770), Expect = 0.0
Identities = 330/446 (73%), Positives = 378/446 (84%), Gaps = 3/446 (0%)
Frame = -1
Query: 1426 TALLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPSYMKNQRARFGKFFKSHLLGSPV 1247
TALLRWNE+RYRKKGLPPGTMGWPVFGETTEFLKQGPS+MKNQRAR+GKFFKSHLLG P
Sbjct: 15 TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 74
Query: 1246 VVSMDPEFNKYILLNEGKGLVPGYPQSMVDILGESNIGGVHGSAHKSLRGTLLAIVSPTM 1067
VSMDPE N+YIL+NE KGLVPGYPQSM+DILG+ NI VHGS HK +RG LLA++SPTM
Sbjct: 75 TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 134
Query: 1066 IREQLLPRLDEFMRSQLSNWDGRTVDIQEKTKEMALLSAHKQI-GIEA--YSEALKPEFD 896
IR QLLP++DEFMRS L+ WD + ++IQEKTKEMALLS+ KQI GIE+ S+ PEF
Sbjct: 135 IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEFMPEFF 194
Query: 895 KLVQGVISLPIDLPGTSYSGGLQARKNMLSILRKTIEERRASSSVQHDMLGCLMRMEDSG 716
KLV G ISLPIDLPGT+Y G QARKN++ +LR+ IEER+AS +DMLGCLMR ++
Sbjct: 195 KLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTNENR 254
Query: 715 QHLSDEVIMDIMITIMYSSYETVSTTTMMAVKHLHDNPKALQRIREEHFEIRKRKAPGDP 536
LSDE I+D++ITI+YS YETVSTT+MMAVK+LHD+P+ L +R+EH IR+RK P DP
Sbjct: 255 YKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRPEDP 314
Query: 535 IDWNDYKSMKFTRAVILETMRLATIVNGVLRKTTHDMELNGYLIPKGWRIYVYMREANYD 356
IDWNDYK M+FTRAVI ET RLATIVNGVLRKTT DMELNG++IPKGWRIYVY RE NYD
Sbjct: 315 IDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREINYD 374
Query: 355 PLLYPEPLTFNPWRWEDKSLEYHNHFMLFGGGIRLCPGKESGIAEISTFLHYFLTRYRWE 176
PLLYP+PL FNPWRW DKSLE N+F+LFGGG R CPGKE GIAEISTFLHYF+TRYRWE
Sbjct: 375 PLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRYRWE 434
Query: 175 EVGGETLEKFPRVRAPKGLHIKVSGY 98
EVGG+ L KFPRV AP GLHI+VS Y
Sbjct: 435 EVGGDKLMKFPRVEAPNGLHIRVSAY 460
>ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinifera]
gi|298204461|emb|CBI16941.3| unnamed protein product
[Vitis vinifera]
Length = 464
Score = 686 bits (1770), Expect = 0.0
Identities = 330/446 (73%), Positives = 378/446 (84%), Gaps = 3/446 (0%)
Frame = -1
Query: 1426 TALLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPSYMKNQRARFGKFFKSHLLGSPV 1247
TALLRWNE+RYRKKGLPPGTMGWPVFGETTEFLKQGPS+MKNQRAR+GKFFKSHLLG P
Sbjct: 19 TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 78
Query: 1246 VVSMDPEFNKYILLNEGKGLVPGYPQSMVDILGESNIGGVHGSAHKSLRGTLLAIVSPTM 1067
VSMDPE N+YIL+NE KGLVPGYPQSM+DILG+ NI VHGS HK +RG LLA++SPTM
Sbjct: 79 TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 138
Query: 1066 IREQLLPRLDEFMRSQLSNWDGRTVDIQEKTKEMALLSAHKQI-GIEA--YSEALKPEFD 896
IR QLLP++DEFMRS L+ WD + ++IQEKTKEMALLS+ KQI GIE+ S+ PEF
Sbjct: 139 IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEFMPEFF 198
Query: 895 KLVQGVISLPIDLPGTSYSGGLQARKNMLSILRKTIEERRASSSVQHDMLGCLMRMEDSG 716
KLV G ISLPIDLPGT+Y G QARKN++ +LR+ IEER+AS +DMLGCLMR ++
Sbjct: 199 KLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTNENR 258
Query: 715 QHLSDEVIMDIMITIMYSSYETVSTTTMMAVKHLHDNPKALQRIREEHFEIRKRKAPGDP 536
LSDE I+D++ITI+YS YETVSTT+MMAVK+LHD+P+ L +R+EH IR+RK P DP
Sbjct: 259 YKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRPEDP 318
Query: 535 IDWNDYKSMKFTRAVILETMRLATIVNGVLRKTTHDMELNGYLIPKGWRIYVYMREANYD 356
IDWNDYK M+FTRAVI ET RLATIVNGVLRKTT DMELNG++IPKGWRIYVY RE NYD
Sbjct: 319 IDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREINYD 378
Query: 355 PLLYPEPLTFNPWRWEDKSLEYHNHFMLFGGGIRLCPGKESGIAEISTFLHYFLTRYRWE 176
PLLYP+PL FNPWRW DKSLE N+F+LFGGG R CPGKE GIAEISTFLHYF+TRYRWE
Sbjct: 379 PLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRYRWE 438
Query: 175 EVGGETLEKFPRVRAPKGLHIKVSGY 98
EVGG+ L KFPRV AP GLHI+VS Y
Sbjct: 439 EVGGDKLMKFPRVEAPNGLHIRVSAY 464
>emb|CAN66537.1| hypothetical protein VITISV_029635 [Vitis vinifera]
Length = 463
Score = 672 bits (1734), Expect = 0.0
Identities = 324/449 (72%), Positives = 372/449 (82%), Gaps = 6/449 (1%)
Frame = -1
Query: 1426 TALLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPSYMKNQRARFGKFFKSHLLGSPV 1247
TALLRWNE+RYRKKGLPPGTMGWPVFGETTEFLKQGPS+MKNQRAR+GKFFKSHLLG P
Sbjct: 15 TALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHLLGCPT 74
Query: 1246 VVSMDPEFNKYILLNEGKGLVPGYPQSMVDILGESNIGGVHGSAHKSLRGTLLAIVSPTM 1067
VSMDPE N+YIL+NE KGLVPGYPQSM+DILG+ NI VHGS HK +RG LLA++SPTM
Sbjct: 75 TVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLALISPTM 134
Query: 1066 IREQLLPRLDEFMRSQLSNWDGRTVDIQEKTKEMALLSAHKQI----GIEA--YSEALKP 905
IR QLLP++DEFMRS L+ WD + ++IQEKTKE+ S Q GIE+ S+ P
Sbjct: 135 IRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEVYGTSLFSQADAVAGIESGTISKEFMP 194
Query: 904 EFDKLVQGVISLPIDLPGTSYSGGLQARKNMLSILRKTIEERRASSSVQHDMLGCLMRME 725
EF KLV G ISLPIDLPGT+Y G QARKN++ +LR+ IEER+AS +DMLGCLMR
Sbjct: 195 EFFKLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMRTN 254
Query: 724 DSGQHLSDEVIMDIMITIMYSSYETVSTTTMMAVKHLHDNPKALQRIREEHFEIRKRKAP 545
++ LSDE I+D++ITI+YS YETVSTT+MMAVK+LHD+P+ L +R+EH IR+RK P
Sbjct: 255 ENRYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERKRP 314
Query: 544 GDPIDWNDYKSMKFTRAVILETMRLATIVNGVLRKTTHDMELNGYLIPKGWRIYVYMREA 365
DPIDWNDYK M+FTRAVI ET RLATIVNGVLRKTT DMELNG++IPKGWRIYVY RE
Sbjct: 315 EDPIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTREI 374
Query: 364 NYDPLLYPEPLTFNPWRWEDKSLEYHNHFMLFGGGIRLCPGKESGIAEISTFLHYFLTRY 185
NYDPLLYP+PL FNPWRW DKSLE N+F+LFGGG R CPGKE GIAEISTFLHYF+TRY
Sbjct: 375 NYDPLLYPDPLAFNPWRWLDKSLESQNYFLLFGGGTRQCPGKELGIAEISTFLHYFVTRY 434
Query: 184 RWEEVGGETLEKFPRVRAPKGLHIKVSGY 98
RWEEVGG+ L KFPRV AP GLHI+VS Y
Sbjct: 435 RWEEVGGDKLMKFPRVEAPNGLHIRVSAY 463
>ref|XP_003554965.1| PREDICTED: cytochrome P450 85A-like [Glycine max]
Length = 465
Score = 668 bits (1723), Expect = 0.0
Identities = 317/446 (71%), Positives = 375/446 (84%), Gaps = 3/446 (0%)
Frame = -1
Query: 1426 TALLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPSYMKNQRARFGKFFKSHLLGSPV 1247
+ALLRWNE+RYRKKGLPPGTMGWP+FGETTEFLKQGP++MK QRAR+G FFKSH+LG P
Sbjct: 20 SALLRWNEVRYRKKGLPPGTMGWPLFGETTEFLKQGPNFMKTQRARYGSFFKSHILGCPT 79
Query: 1246 VVSMDPEFNKYILLNEGKGLVPGYPQSMVDILGESNIGGVHGSAHKSLRGTLLAIVSPTM 1067
+VSMDPE N+YIL+NE KGLVPGYPQSM+DILG+ NI VHGS HK +RG LL+I+SPT+
Sbjct: 80 IVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLSIISPTL 139
Query: 1066 IREQLLPRLDEFMRSQLSNWDGRTVDIQEKTKEMALLSAHKQI-GIEA--YSEALKPEFD 896
IR+QLLP++D+FMR+ LSNW + ++IQEKTKEMA LS+ KQI G+E+ S++ EF
Sbjct: 140 IRDQLLPKIDQFMRAHLSNWGDKVINIQEKTKEMAFLSSLKQIAGMESGSLSDSFMAEFF 199
Query: 895 KLVQGVISLPIDLPGTSYSGGLQARKNMLSILRKTIEERRASSSVQHDMLGCLMRMEDSG 716
KLV G +SLPIDLPGT+Y G QARK +++IL K +EERRAS HDMLGCLM ++S
Sbjct: 200 KLVLGTLSLPIDLPGTNYHSGFQARKTIVNILSKLLEERRASHETYHDMLGCLMGRDESR 259
Query: 715 QHLSDEVIMDIMITIMYSSYETVSTTTMMAVKHLHDNPKALQRIREEHFEIRKRKAPGDP 536
LSDE I+D++ITIMYS YETVSTT+MMAVK+LHD+PKAL+ +R+EH IR+RK P +P
Sbjct: 260 YKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKALEELRKEHLAIRERKKPDEP 319
Query: 535 IDWNDYKSMKFTRAVILETMRLATIVNGVLRKTTHDMELNGYLIPKGWRIYVYMREANYD 356
+D ND KSM+FTRAVI ET RLATIVNGVLRKTT DMELNGYLIPKGWRIYVY RE NYD
Sbjct: 320 LDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTQDMELNGYLIPKGWRIYVYTREINYD 379
Query: 355 PLLYPEPLTFNPWRWEDKSLEYHNHFMLFGGGIRLCPGKESGIAEISTFLHYFLTRYRWE 176
P LYP+PLTFNPWRW DKSLE N+F +FGGG R CPGKE GI EISTFLHYF+TRYRWE
Sbjct: 380 PFLYPDPLTFNPWRWMDKSLESKNYFFIFGGGTRQCPGKELGITEISTFLHYFVTRYRWE 439
Query: 175 EVGGETLEKFPRVRAPKGLHIKVSGY 98
EVGG+ + KFPRV AP GLHI+V+ Y
Sbjct: 440 EVGGDKVMKFPRVEAPNGLHIRVTSY 465
>dbj|BAF56235.1| cytochrome P450 enzyme [Pisum sativum]
Length = 466
Score = 664 bits (1713), Expect = 0.0
Identities = 316/447 (70%), Positives = 373/447 (83%), Gaps = 4/447 (0%)
Frame = -1
Query: 1426 TALLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPSYMKNQRARFGKFFKSHLLGSPV 1247
+ALLRWNE+RYRKKGLPPGTMGWPVFGETTEFLKQGP++MKNQR RFG FFKSH+LG P
Sbjct: 18 SALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPNFMKNQRLRFGSFFKSHILGCPT 77
Query: 1246 VVSMDPEFNKYILLNEGKGLVPGYPQSMVDILGESNIGGVHGSAHKSLRGTLLAIVSPTM 1067
+VSMD E N+YIL+NE KGLVPGYPQSM+DILG+ NI VHGS HK LRG LL+I+SPTM
Sbjct: 78 IVSMDAEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGSTHKYLRGALLSIISPTM 137
Query: 1066 IREQLLPRLDEFMRSQLSNWDGRTVDIQEKTKEMALLSAHKQIG----IEAYSEALKPEF 899
IR+Q+LP++DEFM QLS+WD + ++IQEKTKEM LS+ KQI +++ K EF
Sbjct: 138 IRDQILPKIDEFMSFQLSHWDDKIINIQEKTKEMVFLSSLKQIASMDSTSKTADSFKTEF 197
Query: 898 DKLVQGVISLPIDLPGTSYSGGLQARKNMLSILRKTIEERRASSSVQHDMLGCLMRMEDS 719
KLV G ISLPI+LPGT+Y G QARKN+++ILR+ ++ERRAS+ D+LGCLM E++
Sbjct: 198 FKLVLGTISLPINLPGTNYHRGFQARKNIVNILRELLKERRASNETHKDILGCLMENEEN 257
Query: 718 GQHLSDEVIMDIMITIMYSSYETVSTTTMMAVKHLHDNPKALQRIREEHFEIRKRKAPGD 539
LSDE I+D++IT+MYS YETVSTT+MMAVK+LHD+PKAL+ IR+EH IR+RK P +
Sbjct: 258 KYKLSDEEIIDLVITLMYSGYETVSTTSMMAVKYLHDHPKALEEIRKEHLAIRERKKPNE 317
Query: 538 PIDWNDYKSMKFTRAVILETMRLATIVNGVLRKTTHDMELNGYLIPKGWRIYVYMREANY 359
PID+ND KSM+FTRAVI ET RLATIVNGVLRKTT DMELNGYLIPKGWRIYVY RE NY
Sbjct: 318 PIDFNDIKSMRFTRAVIFETSRLATIVNGVLRKTTQDMELNGYLIPKGWRIYVYTREINY 377
Query: 358 DPLLYPEPLTFNPWRWEDKSLEYHNHFMLFGGGIRLCPGKESGIAEISTFLHYFLTRYRW 179
DP LYPEPL FNPWRW DKSLE N+F++FGGG RLCPGKE+GI EISTFLHY LTRYRW
Sbjct: 378 DPFLYPEPLAFNPWRWMDKSLESSNYFLIFGGGTRLCPGKEAGITEISTFLHYLLTRYRW 437
Query: 178 EEVGGETLEKFPRVRAPKGLHIKVSGY 98
EE GG+ L KFPRV+AP GLH+K S +
Sbjct: 438 EETGGDKLMKFPRVQAPNGLHMKFSSF 464