BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00007855
         (1851 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAJ40864.1| tetrahydroberberine oxidase [Coptis japonica]        1022   0.0  
gb|ADY15026.1| (S)-tetrahydroprotoberberine oxidase [Berberis wi...   687   0.0  
ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|2...   582   e-163
ref|XP_004135722.1| PREDICTED: reticuline oxidase-like protein-l...   563   e-158
ref|XP_004158101.1| PREDICTED: reticuline oxidase-like protein-l...   560   e-157

>dbj|BAJ40864.1| tetrahydroberberine oxidase [Coptis japonica]
          Length = 540

 Score = 1022 bits (2642), Expect = 0.0
 Identities = 512/540 (94%), Positives = 512/540 (94%)
 Frame = -1

Query: 1800 MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRXXXXXXXXXXXXXXYTSI 1621
            MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLR              YTSI
Sbjct: 1    MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPIYNPSSTSYTSI 60

Query: 1620 LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVA 1441
            LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVA
Sbjct: 61   LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVA 120

Query: 1440 TVPFVLLDLIHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG 1261
            TVPFVLLDLIHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG
Sbjct: 121  TVPFVLLDLIHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG 180

Query: 1260 HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA 1081
            HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA
Sbjct: 181  HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA 240

Query: 1080 WKIRLXXXXXXXXXXXXXXVLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE 901
            WKIRL              VLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE
Sbjct: 241  WKIRLVPVPPTVTTFVVPRVLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE 300

Query: 900  KKTIVISFLGFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSL 721
            KKTIVISFLGFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSL
Sbjct: 301  KKTIVISFLGFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSL 360

Query: 720  LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFNEVDEPEIIMSPYGGKMNEIEES 541
            LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFNEVDEPEIIMSPYGGKMNEIEES
Sbjct: 361  LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFNEVDEPEIIMSPYGGKMNEIEES 420

Query: 540  AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDN 361
            AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDN
Sbjct: 421  AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDN 480

Query: 360  DLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLRSLAEAKK 181
            DLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLRSLAEAKK
Sbjct: 481  DLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLRSLAEAKK 540


>gb|ADY15026.1| (S)-tetrahydroprotoberberine oxidase [Berberis wilsonae]
          Length = 530

 Score =  687 bits (1772), Expect = 0.0
 Identities = 340/522 (65%), Positives = 409/522 (78%), Gaps = 4/522 (0%)
 Frame = -1

Query: 1752 AIANSLVP--LASSSSEYEGFLQCLDLRXXXXXXXXXXXXXXYTSILHASIYNLRFISPT 1579
            ++ +SL+P  LASS + YE FLQCLDL               YTSIL ++I NL F+SPT
Sbjct: 13   SLLSSLLPTSLASSDANYEDFLQCLDLYSQNSIPVYTRNTSSYTSILESTIKNLVFLSPT 72

Query: 1578 TPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVATVPFVLLDLIHLRT 1399
            TPKPNFI+TPM+ESHVQ +V+C R HGL +R RSGGHDFEG S+V+ VPFV+LDLIHL+T
Sbjct: 73   TPKPNFIVTPMQESHVQTSVICCRMHGLQMRIRSGGHDFEGLSYVSNVPFVVLDLIHLKT 132

Query: 1398 ISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGILMRKY 1219
            I+V+IE+ +AWVQTGATIGELYYRIAEK   HAFPAGLCP+VGVGGHISGAGYG+LMRKY
Sbjct: 133  INVDIEENSAWVQTGATIGELYYRIAEKVGVHAFPAGLCPTVGVGGHISGAGYGVLMRKY 192

Query: 1218 GLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKIRLXXXXXXXXX 1039
            G+SADHVIDAR+V+V+G ILD+ESMGED FWAIRGGGGASFGVILAWKIRL         
Sbjct: 193  GVSADHVIDARIVNVDGEILDRESMGEDLFWAIRGGGGASFGVILAWKIRLVPVPPTVTI 252

Query: 1038 XXXXXVLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPEKKTIVISFLGFYLG 859
                  LE+GATAL+HKWQ I D + +DLF+GLS++++ + P     KTI++SF+G +LG
Sbjct: 253  FIVPKTLEEGATALLHKWQFIGDNVHEDLFIGLSMRSVIISP--KGDKTILVSFIGLFLG 310

Query: 858  APEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSLLLERKNPIPPKNRY 679
              +K +Q ME+SFPELG+   DCIEMSWIKS + FG+F  +  LS+LL+RKNP PPK+ +
Sbjct: 311  GSDKLVQHMEQSFPELGVKPHDCIEMSWIKSTVVFGVFSNDASLSVLLDRKNPFPPKSYH 370

Query: 678  MSKSDYVHEPVSEAVLEGIWKRF--NEVDEPEIIMSPYGGKMNEIEESAIAFPHRKGNMY 505
              KSDYV EP+  +VLEGI  RF  N V++ EIIMSPYGG+MNEI ES IAFPHRKGN+Y
Sbjct: 371  KVKSDYVTEPLPISVLEGICHRFLKNGVNKAEIIMSPYGGRMNEISESEIAFPHRKGNLY 430

Query: 504  KINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDNDLGHKKNGTATY 325
            KINY+  W+E G  + + H SWIRELY YMTPYVSK+PR SYLNFKD DLG  KNGTATY
Sbjct: 431  KINYIAEWEEAG--SMENHLSWIRELYRYMTPYVSKSPRSSYLNFKDIDLGQTKNGTATY 488

Query: 324  LEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLRS 199
             +AK WGSKY+KNNF RL+ VKTKVDP++FF NEQ IPP  S
Sbjct: 489  SQAKAWGSKYFKNNFKRLMQVKTKVDPNNFFCNEQGIPPFSS 530


>ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|222875303|gb|EEF12434.1|
            predicted protein [Populus trichocarpa]
          Length = 527

 Score =  582 bits (1500), Expect = e-163
 Identities = 292/528 (55%), Positives = 378/528 (71%), Gaps = 5/528 (0%)
 Frame = -1

Query: 1767 FLVFIAIANSLVPLASSSSEYEGFLQCLDLRXXXXXXXXXXXXXXYT--SILHASIYNLR 1594
            FL F+  + S V   +S+  +E FLQCLD +               +  SIL  SI NLR
Sbjct: 4    FLFFLIFSFSWV---TSAHTHESFLQCLDSQNSHSISKLIYTPINSSYSSILQFSIQNLR 60

Query: 1593 FISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVATVPFVLLDL 1414
            F + +TPKP  I+TP   SH+QA ++CS++HG+ IR RSGGHD+EG S+V+T  FV++DL
Sbjct: 61   FNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDL 120

Query: 1413 IHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGI 1234
            I+LRTI+VN E++TAWVQ+G TIGE+YYRIAEKSRT AFPA +CP+VGVGGH SG GYG+
Sbjct: 121  INLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGM 180

Query: 1233 LMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKIRLXXXX 1054
            LMRKYGL+ADH+IDA+LVDV GRILD+ESMGED FWAIRGGGG +FGV++AWKI L    
Sbjct: 181  LMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAP 240

Query: 1053 XXXXXXXXXXVLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPEKKTIVISFL 874
                       LEQ A+ LVH+WQ +ADKL +DLF+ + +  ++   +     TI  SF 
Sbjct: 241  PTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEEGKSNTTIQASFN 300

Query: 873  GFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSLLLERKNPIP 694
              +LG  ++ L L+++SFPELGL+KEDCIEMSWI+S LYF  F   + L +LL+R  P  
Sbjct: 301  SLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDR-TPST 359

Query: 693  PKNRYMSKSDYVHEPVSEAVLEGIWKRF--NEVDEPEIIMSPYGGKMNEIEESAIAFPHR 520
             +N + +KSDYV EP+ E  LEGIW+RF   +++ P +I SPYGGKM+EI ES+I FPHR
Sbjct: 360  RRN-FKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPHR 418

Query: 519  KGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDNDLG-HKK 343
             GN+YKI +L+ W E G  A K+H SWIR LY+Y+ PYVSK PR +Y+N++D D+G +  
Sbjct: 419  AGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGINNH 478

Query: 342  NGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLRS 199
             G  +Y +A +WG KY+KNNFDRLV VKT VDP +FF NEQSIPPL S
Sbjct: 479  AGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLSS 526


>ref|XP_004135722.1| PREDICTED: reticuline oxidase-like protein-like [Cucumis sativus]
          Length = 543

 Score =  563 bits (1450), Expect = e-158
 Identities = 276/517 (53%), Positives = 373/517 (72%), Gaps = 9/517 (1%)
 Frame = -1

Query: 1725 ASSSSEYEGFLQCLDLRXXXXXXXXXXXXXXY----TSILHASIYNLRFISPTTPKPNFI 1558
            A+S+  +E FL CL                      +S+L+ SI+NLRF SP TPKP  I
Sbjct: 20   AASAHNHESFLHCLSHHSPNTSSISKIIYTPTNPSYSSVLNFSIHNLRFTSPKTPKPQVI 79

Query: 1557 ITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVATVPFVLLDLIHLRTISVNIED 1378
            +TP   S +QA+++C++  G  IRTRSGGHD+EG S+V+ V FV++DLI+LR+ISV+ E+
Sbjct: 80   VTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLSYVSDVSFVVVDLINLRSISVDAEN 139

Query: 1377 ETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGILMRKYGLSADHV 1198
              AWVQ+GATIGELYYRIAEKS+T  FPAG+CP+VGVGGH SG GYG+++RK+GL+AD+V
Sbjct: 140  NVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAADNV 199

Query: 1197 IDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKIRLXXXXXXXXXXXXXXVL 1018
            IDA  +DVNG++ D++SMGED FWAIRG GGAS+G++LAWKI+L               L
Sbjct: 200  IDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGIVLAWKIKLLPVPPIVTVFTIARTL 259

Query: 1017 EQGATALVHKWQIIADKLDDDLFLGLSVQALH-LDPDHPEKKTIVISFLGFYLGAPEKTL 841
            EQ AT ++H+WQ ++ K DD LF+ + +  ++  +  H  K+TI  +F   +LG  E+ +
Sbjct: 260  EQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNSQHGNKRTIEAAFNSLFLGKIEELV 319

Query: 840  QLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSLLLERKNPIPPKNRYMSKSDY 661
             +M+++FPELGL KEDCIEMSWI+S LYF  F     L++LL+R+ P+ PK  + +KSDY
Sbjct: 320  PIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQPLNVLLDRR-PLTPKRFFKAKSDY 378

Query: 660  VHEPVSEAVLEGIWKRFN--EVDEPEIIMSPYGGKMNEIEESAIAFPHRKGNMYKINYLV 487
            V+EP+ +A LEGIW+ FN  E +   +I+SPYGG M++I ES I FPHR GN+YKI +LV
Sbjct: 379  VNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIMDKISESEIPFPHRAGNLYKIQHLV 438

Query: 486  SWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSYLNFKDNDLG-HKKNGTAT-YLEAK 313
             W E GE+  K+H +WIR+LY+YM P+VSKNPR +Y+N++D D+G ++KNG  T Y EA 
Sbjct: 439  YWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAYINYRDLDIGTNEKNGRNTSYNEAS 498

Query: 312  VWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPLR 202
            VWG KY+K NF RLVSVKTKVDP +FF NEQSIP L+
Sbjct: 499  VWGIKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSLK 535


>ref|XP_004158101.1| PREDICTED: reticuline oxidase-like protein-like, partial [Cucumis
            sativus]
          Length = 513

 Score =  560 bits (1444), Expect = e-157
 Identities = 269/481 (55%), Positives = 363/481 (75%), Gaps = 5/481 (1%)
 Frame = -1

Query: 1629 TSILHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSS 1450
            +S+L+ SI+NLRF SP TPKP  I+TP   S +QA+++C++  G  IRTRSGGHD+EG S
Sbjct: 26   SSVLNFSIHNLRFTSPKTPKPQVIVTPFHVSQIQASIICAKNTGFQIRTRSGGHDYEGLS 85

Query: 1449 FVATVPFVLLDLIHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVG 1270
            +V+ V FV++DLI+LR+ISV+ E+  AWVQ+GATIGELYYRIAEKS+T  FPAG+CP+VG
Sbjct: 86   YVSDVSFVVVDLINLRSISVDAENNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVG 145

Query: 1269 VGGHISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGV 1090
            VGGH SG GYG+++RK+GL+AD+VIDA  +DVNG++ D++SMGED FWAIRG GGAS+G+
Sbjct: 146  VGGHFSGGGYGLMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGSGGASYGI 205

Query: 1089 ILAWKIRLXXXXXXXXXXXXXXVLEQGATALVHKWQIIADKLDDDLFLGLSVQALH-LDP 913
            +LAWKI+L               LEQ AT ++H+WQ ++ K DD LF+ + +  ++  + 
Sbjct: 206  VLAWKIKLLPVPPIVTVFTIARTLEQNATDIIHRWQYVSSKQDDKLFIRIILTGINTTNS 265

Query: 912  DHPEKKTIVISFLGFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLET 733
             H  K+TI  +F   +LG  E+ + +M+++FPELGL KEDCIEMSWI+S LYF  F    
Sbjct: 266  QHGNKRTIEAAFNSLFLGKIEELVPIMQKTFPELGLTKEDCIEMSWIESVLYFAGFSRGQ 325

Query: 732  DLSLLLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFN--EVDEPEIIMSPYGGKM 559
             L++LL+R+ P+ PK  + +KSDYV+EP+ +A LEGIW+ FN  E +   +I+SPYGG M
Sbjct: 326  PLNVLLDRR-PLTPKRFFKAKSDYVNEPIPKAGLEGIWEFFNEEEAEAAVLILSPYGGIM 384

Query: 558  NEIEESAIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVSKNPRCSY 379
            ++I ES I FPHR GN+YKI +LV W E GE+  K+H +WIR+LY+YM P+VSKNPR +Y
Sbjct: 385  DKISESEIPFPHRAGNLYKIQHLVYWDEEGEDIAKRHINWIRKLYSYMAPFVSKNPRAAY 444

Query: 378  LNFKDNDLG-HKKNGTAT-YLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTNEQSIPPL 205
            +N++D D+G ++KNG  T Y EA VWG KY+K NF RLVSVKTKVDP +FF NEQSIP L
Sbjct: 445  INYRDLDIGTNEKNGRNTSYNEASVWGIKYFKGNFKRLVSVKTKVDPSNFFKNEQSIPSL 504

Query: 204  R 202
            +
Sbjct: 505  K 505