BLASTX nr result
ID: Coptis21_contig00007629
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00007629
(1791 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis ... 272 e-133
ref|XP_002510136.1| cytochrome P450, putative [Ricinus communis]... 257 e-131
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]... 275 e-130
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V... 271 e-128
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum] 277 e-128
>ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis vinifera]
Length = 507
Score = 272 bits (695), Expect(2) = e-133
Identities = 127/234 (54%), Positives = 177/234 (75%), Gaps = 1/234 (0%)
Frame = +1
Query: 1000 ILDALLSIQEAEPEHYSDKIIKGIILLLFAAGTDTTSLTLEWAFSLLVNNPDILHKARSE 1179
+++ LL +Q++EP+ Y+D +IKG++L++ +AGTDT+S+TLEWA SLL+N+P+ L KAR+E
Sbjct: 275 LIETLLFLQQSEPDFYTDDVIKGLMLVVISAGTDTSSVTLEWAMSLLLNHPEALEKARAE 334
Query: 1180 IDNHAQ-GRLLDNYDLAKLPYLHCIVNEXXXXXXXXXXXXXHVSSEECIVGGFRIPCGTI 1356
ID+H + G LLD+ DLAKLPYL +VNE H+SSE+C VGGF IP GT
Sbjct: 335 IDSHVKPGHLLDDSDLAKLPYLRSVVNETLRLYPTAPLLLPHLSSEDCSVGGFDIPRGTT 394
Query: 1357 LFANIWAIHRDPKVWVEPTKFMPERFEGVQGEKELGVKFSPFGLGRRGCPGAGMAIRMLA 1536
+ N+WA+HRDP+VW E TKF PERFEG++ E++ KF+PFG+GRR CPGA +A+++++
Sbjct: 395 VMVNVWALHRDPRVWEEATKFKPERFEGMENEEKEAFKFAPFGIGRRACPGAALAMKIVS 454
Query: 1537 LALGTLIQCFEWERVGEEQVEMNEKNTGATMPKAMPLVVTCKPHPKMVNALSQI 1698
LALG LIQCFEWERV E+V+M+ +G TMPKA PL + +P P M + LSQ+
Sbjct: 455 LALGGLIQCFEWERVEAEKVDMS-PGSGITMPKAKPLEIIFRPRPTMTSLLSQL 507
Score = 230 bits (586), Expect(2) = e-133
Identities = 112/234 (47%), Positives = 151/234 (64%), Gaps = 1/234 (0%)
Frame = +3
Query: 201 LPPSPFAIPILGHLHLFKQPLNRTLQTLSDKYGPVLFLKFGARRXXXXXXXXXVEECFTK 380
LPPSPFA+P++GHLHL K PL + LQ LS +YGP+LFL+FG R V+ECFTK
Sbjct: 32 LPPSPFALPVIGHLHLLKPPLYQGLQALSSQYGPILFLRFGCRPFVVVSSPSAVQECFTK 91
Query: 381 NDIVFANRPHTVTVGHLNYNYTTVAFAPYGDLWRNIRRITTIEIFSSKSLQMSSTIRREE 560
ND+V ANRP ++ H+ YNYT A+A YG LWR +RR+T +EI SS L + ST+R EE
Sbjct: 92 NDVVLANRPRSMIGDHVTYNYTAFAWASYGHLWRVLRRLTVVEILSSNKLLLLSTVREEE 151
Query: 561 IHCLIKNLFQSSKRGLHHFNLKLMFSKLMFNVMMKMVAGKRCF-EECMGLDEQKRLHVHL 737
+ L++ LF+ S G +++L S FN +MK + GKRC EE G++ ++ L
Sbjct: 152 VRYLLRQLFKVSNDGAQKVDMRLYLSLFSFNFIMKTITGKRCIEEEAEGIETNRQFLERL 211
Query: 738 KECFMPRQLLNMEDLLPILRWIGLYRREEKFVINLQKMRDSFLQEMIDDYRRKN 899
K F+P N+ D PILRW+G Y+ EK VI K RD +LQ M+D++RR N
Sbjct: 212 KRIFVPTTTTNLCDFFPILRWVG-YKGLEKSVIQFGKERDGYLQGMLDEFRRNN 264
>ref|XP_002510136.1| cytochrome P450, putative [Ricinus communis]
gi|223550837|gb|EEF52323.1| cytochrome P450, putative
[Ricinus communis]
Length = 505
Score = 257 bits (657), Expect(2) = e-131
Identities = 129/234 (55%), Positives = 165/234 (70%), Gaps = 1/234 (0%)
Frame = +1
Query: 1000 ILDALLSIQEAEPEHYSDKIIKGIILLLFAAGTDTTSLTLEWAFSLLVNNPDILHKARSE 1179
+ + LLS+QE EP Y+D +IK I++++F AG +T+++ LEWA SLL+ +P+IL K R+E
Sbjct: 274 VAETLLSLQELEPAIYTDDVIKSIMVMMFIAGVETSAVALEWAMSLLLIHPEILQKLRAE 333
Query: 1180 IDNHA-QGRLLDNYDLAKLPYLHCIVNEXXXXXXXXXXXXXHVSSEECIVGGFRIPCGTI 1356
IDN GRLL++ DL KLPYL C+VNE H+SSE C VGGF I GT+
Sbjct: 334 IDNCVGHGRLLNDLDLVKLPYLRCVVNETLRLYPPGPLMLPHLSSEICTVGGFEIQKGTL 393
Query: 1357 LFANIWAIHRDPKVWVEPTKFMPERFEGVQGEKELGVKFSPFGLGRRGCPGAGMAIRMLA 1536
L N+WA+HRDP VW EPT+F PERFEG GE E KF PFG+GRR CPGAGM RM++
Sbjct: 394 LMVNLWAMHRDPNVWEEPTEFKPERFEGDLGE-EHAFKFMPFGMGRRACPGAGMGTRMVS 452
Query: 1537 LALGTLIQCFEWERVGEEQVEMNEKNTGATMPKAMPLVVTCKPHPKMVNALSQI 1698
LALG LIQ FEWE+ G E+V+MN + G ++ KA PLVV C P P+MV LSQ+
Sbjct: 453 LALGALIQSFEWEKDGLEKVDMNPR-FGMSLSKAKPLVVLCCPRPEMVEVLSQL 505
Score = 241 bits (615), Expect(2) = e-131
Identities = 120/249 (48%), Positives = 164/249 (65%), Gaps = 1/249 (0%)
Frame = +3
Query: 174 KLLLQANKNLPPSPFAIPILGHLHLFKQPLNRTLQTLSDKYGPVLFLKFGARRXXXXXXX 353
K+L +NLPP PF++P++GHLH+ K+PL L+TL KYGP+L LKFG R
Sbjct: 19 KILFPQKQNLPPGPFSLPLIGHLHMLKKPLPLALETLLSKYGPILSLKFGYRSVLVVSSP 78
Query: 354 XXVEECFTKNDIVFANRPHTVTVGHLNYNYTTVAFAPYGDLWRNIRRITTIEIFSSKSLQ 533
VEECFTKNDI+FANRP ++ H YNYTT +APYG+LWR++RR+T +E+FSSKSLQ
Sbjct: 79 SAVEECFTKNDIIFANRPKSMAGDHFTYNYTTYVWAPYGELWRSLRRLTVVELFSSKSLQ 138
Query: 534 MSSTIRREEIHCLIKNLFQSSKRGLHHFNLKLMFSKLMFNVMMKMVAGKRCF-EECMGLD 710
+S IR++E+H L+ LF+ S G +LK + S LM NVMMK+ GK C EE G +
Sbjct: 139 NNSAIRKQEVHRLVSRLFKVSAGGKQKVDLKFLLSLLMCNVMMKIAVGKLCVKEEIEGTE 198
Query: 711 EQKRLHVHLKECFMPRQLLNMEDLLPILRWIGLYRREEKFVINLQKMRDSFLQEMIDDYR 890
+K+L+ K+ F P LN+ D +P LR IG ++ EK +I +Q RD FLQ+++D R
Sbjct: 199 VEKQLYQGFKDKFFPSLTLNVCDFIPFLRMIG-FKGIEKNMIKMQNKRDEFLQDLLDGIR 257
Query: 891 RKNIRSSTT 917
S T+
Sbjct: 258 LNRKHSKTS 266
>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
gi|223550831|gb|EEF52317.1| cytochrome P450, putative
[Ricinus communis]
Length = 500
Score = 275 bits (702), Expect(2) = e-130
Identities = 127/234 (54%), Positives = 171/234 (73%), Gaps = 1/234 (0%)
Frame = +1
Query: 1000 ILDALLSIQEAEPEHYSDKIIKGIILLLFAAGTDTTSLTLEWAFSLLVNNPDILHKARSE 1179
+++ LLS+QE+EPE+Y+D+ IK ++L+L AAGTDT++ T+EWA SLLVNNP+ L KA++E
Sbjct: 269 MIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTSAATMEWAMSLLVNNPEALKKAQTE 328
Query: 1180 IDNH-AQGRLLDNYDLAKLPYLHCIVNEXXXXXXXXXXXXXHVSSEECIVGGFRIPCGTI 1356
ID+ RL++ D +KLPYL+CI+NE H SSEEC +GG+R+P GT+
Sbjct: 329 IDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAGPLLVPHESSEECFIGGYRVPAGTM 388
Query: 1357 LFANIWAIHRDPKVWVEPTKFMPERFEGVQGEKELGVKFSPFGLGRRGCPGAGMAIRMLA 1536
L N+W+I DP+VW EP F PERFEG +G ++ G + PFG GRR CPG G+A+RM+
Sbjct: 389 LLVNLWSIQNDPRVWEEPRNFKPERFEGCEGVRD-GFRLMPFGSGRRSCPGEGLALRMVG 447
Query: 1537 LALGTLIQCFEWERVGEEQVEMNEKNTGATMPKAMPLVVTCKPHPKMVNALSQI 1698
L +GTL+QCF+WERVG+E ++M E G TMPKA PLVV C P P MVN LSQ+
Sbjct: 448 LGIGTLLQCFDWERVGKEMIDMTE-GVGLTMPKAQPLVVQCSPRPSMVNLLSQL 500
Score = 219 bits (557), Expect(2) = e-130
Identities = 111/236 (47%), Positives = 161/236 (68%), Gaps = 2/236 (0%)
Frame = +3
Query: 195 KNLPPSPF-AIPILGHLHLFKQPLNRTLQTLSDKYGPVLFLKFGARRXXXXXXXXXVEEC 371
+N PPSPF +P+LGHLHL K+PL+R+L TLS++YGPVL L+FG RR EEC
Sbjct: 24 RNHPPSPFPTLPLLGHLHLLKKPLHRSLSTLSNRYGPVLLLQFGYRRVLVVSSPSAAEEC 83
Query: 372 FTKNDIVFANRPHTVTVGHLNYNYTTVAFAPYGDLWRNIRRITTIEIFSSKSLQMSSTIR 551
TK D VFANRP + H+ YNYT++A+APYGDLWRN+R+++ +EI SS LQ+ S+IR
Sbjct: 84 LTKCDTVFANRPRLLAGKHIGYNYTSLAWAPYGDLWRNLRKVSALEILSSHRLQLLSSIR 143
Query: 552 REEIHCLIKNLFQSSKRGLHHFNLKLMFSKLMFNVMMKMVAGKRCF-EECMGLDEQKRLH 728
R+E+ LI+ LF+++K ++K F +LM NVMM+M+AGKR + E ++E KR
Sbjct: 144 RDEVKLLIQRLFRNNKDCREKVDMKSAFFELMLNVMMRMIAGKRYYGENVEQVEEAKRFR 203
Query: 729 VHLKECFMPRQLLNMEDLLPILRWIGLYRREEKFVINLQKMRDSFLQEMIDDYRRK 896
++E F+ NM D LP++ +G +EK + L K RD F+Q++ID++R++
Sbjct: 204 EIVRETFLAAGTSNMGDFLPLVAVVG---GQEKRLKELGKRRDGFIQDLIDEHRKR 256
>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
Length = 503
Score = 271 bits (693), Expect(2) = e-128
Identities = 131/234 (55%), Positives = 172/234 (73%), Gaps = 1/234 (0%)
Frame = +1
Query: 1000 ILDALLSIQEAEPEHYSDKIIKGIILLLFAAGTDTTSLTLEWAFSLLVNNPDILHKARSE 1179
+LD LLS+QE EPE Y+D II+G++ ++ +AGTDT++ T+EWA SLL+NNP+ L KA++E
Sbjct: 272 MLDVLLSLQETEPECYTDDIIRGMMQVMLSAGTDTSAGTMEWAMSLLLNNPEALEKAQAE 331
Query: 1180 IDNH-AQGRLLDNYDLAKLPYLHCIVNEXXXXXXXXXXXXXHVSSEECIVGGFRIPCGTI 1356
ID+H + RL+D D+A+LPYL I+ E H SSEEC VGGFR+P GT+
Sbjct: 332 IDSHLGKSRLIDELDIAELPYLRGIIKETLRMYPAAPLLVPHESSEECTVGGFRVPSGTM 391
Query: 1357 LFANIWAIHRDPKVWVEPTKFMPERFEGVQGEKELGVKFSPFGLGRRGCPGAGMAIRMLA 1536
L N+WAI DP +W EP+KF PERF+G +G++ G FSPFG GRRGCPG G+A+R++
Sbjct: 392 LLVNMWAIQNDPMLWAEPSKFKPERFQGPEGQRN-GFMFSPFGAGRRGCPGEGLAMRVVG 450
Query: 1537 LALGTLIQCFEWERVGEEQVEMNEKNTGATMPKAMPLVVTCKPHPKMVNALSQI 1698
LALG+LIQ FEWERV EE V+M+E TG TMPKA LV C+P P MV+ LSQ+
Sbjct: 451 LALGSLIQFFEWERVDEEMVDMSE-GTGLTMPKAQSLVAKCRPRPSMVSLLSQL 503
Score = 217 bits (552), Expect(2) = e-128
Identities = 113/245 (46%), Positives = 159/245 (64%), Gaps = 3/245 (1%)
Frame = +3
Query: 168 MRKLLLQANKNLPPSP-FAIPILGHLHLFKQPLNRTLQTLSDKYGPVLFLKFGARRXXXX 344
+ K Q+NK LPPSP ++PI+GHLHLFK+PL+RT +S++YGP+LF++FG+R
Sbjct: 17 LTKHFFQSNKKLPPSPPLSLPIIGHLHLFKKPLHRTFAKISNQYGPILFIRFGSRPVIIV 76
Query: 345 XXXXXVEECFTKNDIVFANRPHTVTVGHLNYNYTTVAFAPYGDLWRNIRRITTIEIFSSK 524
EECFTKNDIVFANRP + HL YNYTT+ +APYG WRN+RRI ++EI SS
Sbjct: 77 SSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYTTLTWAPYGQHWRNLRRIASLEILSSN 136
Query: 525 SLQMSSTIRREEIHCLIKNLFQSSKRG-LHHFNLKLMFSKLMFNVMMKMVAGKRCF-EEC 698
LQM IR +E+ L+ LF++S G ++K MF +L N MM+M++GKR + +
Sbjct: 137 RLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDMKSMFFELTLNNMMRMISGKRYYGDNV 196
Query: 699 MGLDEQKRLHVHLKECFMPRQLLNMEDLLPILRWIGLYRREEKFVINLQKMRDSFLQEMI 878
L+E ++ + E F N+ D +P +WIGL E+K VI LQ RD F+Q +I
Sbjct: 197 TELEETRKFREIVAETFELSGATNIVDFVPFSKWIGLNGIEKKLVI-LQGKRDGFMQNLI 255
Query: 879 DDYRR 893
+++RR
Sbjct: 256 EEHRR 260
>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
Length = 507
Score = 277 bits (709), Expect(2) = e-128
Identities = 131/234 (55%), Positives = 168/234 (71%), Gaps = 1/234 (0%)
Frame = +1
Query: 1000 ILDALLSIQEAEPEHYSDKIIKGIILLLFAAGTDTTSLTLEWAFSLLVNNPDILHKARSE 1179
++D LLS+QE EPE+Y+D++I+G+I +LFAAGTDT++ T+EWA SLL+NNPD++ KA+SE
Sbjct: 275 LIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSE 334
Query: 1180 IDNHAQ-GRLLDNYDLAKLPYLHCIVNEXXXXXXXXXXXXXHVSSEECIVGGFRIPCGTI 1356
ID + GR +D DL KLPYLHCI+NE H SSE+C +GG+ IPCGT+
Sbjct: 335 IDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTM 394
Query: 1357 LFANIWAIHRDPKVWVEPTKFMPERFEGVQGEKELGVKFSPFGLGRRGCPGAGMAIRMLA 1536
L N+WAI DP +W EP KF PERFEG QG G K PFG GRRGCPG G+A R++
Sbjct: 395 LLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVG 454
Query: 1537 LALGTLIQCFEWERVGEEQVEMNEKNTGATMPKAMPLVVTCKPHPKMVNALSQI 1698
L L +L+QCF+W RVGEE VEM+E TG T+PK PL C+P M+N LSQ+
Sbjct: 455 LTLXSLLQCFDWHRVGEEMVEMSE-GTGLTLPKLHPLEAHCRPRSTMLNFLSQV 507
Score = 209 bits (533), Expect(2) = e-128
Identities = 112/277 (40%), Positives = 169/277 (61%), Gaps = 3/277 (1%)
Frame = +3
Query: 93 IWFICFLCFIMEXXXXXXXXXXXXXMRKLLLQA--NKNLPPSPFAIPILGHLHLFKQPLN 266
++F+ F+ F++ + KL+ + +KNLPPSP ++PI+GHLHLFK+PL
Sbjct: 6 VYFLLFITFLL--------------LSKLVFSSPKHKNLPPSPPSLPIIGHLHLFKKPLY 51
Query: 267 RTLQTLSDKYGPVLFLKFGARRXXXXXXXXXVEECFTKNDIVFANRPHTVTVGHLNYNYT 446
RTL +S++YGPVL+ +FG+R EEC TKNDI FANRP + H NYT
Sbjct: 52 RTLTNISNQYGPVLYFQFGSRPVLVVSSPSAAEECLTKNDIGFANRPRLMIGKHSGNNYT 111
Query: 447 TVAFAPYGDLWRNIRRITTIEIFSSKSLQMSSTIRREEIHCLIKNLFQSSKRGLHHFNLK 626
T+A+A YG WRN+RRI+ +E+FS +QM S IR +E+ +++ L + H +K
Sbjct: 112 TLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLVAGYE--YHKVEMK 169
Query: 627 LMFSKLMFNVMMKMVAGKRCF-EECMGLDEQKRLHVHLKECFMPRQLLNMEDLLPILRWI 803
+F +L N+MM+M+AGKR + E L++ ++ ++E F N+ D LP+LRWI
Sbjct: 170 SVFFELTLNIMMRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLRWI 229
Query: 804 GLYRREEKFVINLQKMRDSFLQEMIDDYRRKNIRSST 914
G Y+ EK +I L+ RD FLQE+I + R+ + S +
Sbjct: 230 G-YKGIEKRLIRLKNKRDEFLQELIQERRKLRVNSKS 265