BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00007184
         (1880 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]      1054   0.0  
gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver...   512   e-142
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    486   e-135
ref|XP_002284806.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   485   e-134
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           483   e-133

>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 534

 Score = 1054 bits (2725), Expect = 0.0
 Identities = 522/534 (97%), Positives = 522/534 (97%)
 Frame = -2

Query: 1801 MHNPTLKQWIRTSTLPAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNL 1622
            MHNPTLKQWIRTSTLPAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNL
Sbjct: 1    MHNPTLKQWIRTSTLPAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNL 60

Query: 1621 LSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAI 1442
            LSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAI
Sbjct: 61   LSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAI 120

Query: 1441 KYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTK 1262
            KYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTK
Sbjct: 121  KYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTK 180

Query: 1261 RKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMDVMVFSDV 1082
            RKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMDVMVFSDV
Sbjct: 181  RKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMDVMVFSDV 240

Query: 1081 IPYLGWLDRLRGVDSEIKRTAKELDSVLESWVDEHRQKRVSISAGIGGIVNXXXXXXXXX 902
            IPYLGWLDRLRGVDSEIKRTAKELDS LESWVDEHRQKRVSISAGIGGIVN         
Sbjct: 241  IPYLGWLDRLRGVDSEIKRTAKELDSALESWVDEHRQKRVSISAGIGGIVNITEEEEIDF 300

Query: 901  XXIMLSIIAKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQC 722
              IMLSIIAKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQC
Sbjct: 301  IDIMLSIIAKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQC 360

Query: 721  ELDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTR 542
            ELDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTR
Sbjct: 361  ELDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTR 420

Query: 541  LWINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSL 362
            LWINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSL
Sbjct: 421  LWINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSL 480

Query: 361  QVMHLVLARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPRFPPVFYM 200
            QVMHLVLARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPRFPPVFYM
Sbjct: 481  QVMHLVLARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPRFPPVFYM 534


>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
          Length = 526

 Score =  512 bits (1318), Expect = e-142
 Identities = 260/515 (50%), Positives = 350/515 (67%), Gaps = 5/515 (0%)
 Frame = -2

Query: 1750 PIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNLLSVPKPAYIVLGELADQ 1571
            PI V+ +VI L + + +R+      K APE  G  P++GHL+L +  +  +  LG +AD 
Sbjct: 21   PISVALVVIALVWNYGRRNPTK---KLAPEASGGRPIMGHLHLFNDGELTHRKLGAMADT 77

Query: 1570 YGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGP 1391
            YGP F+I+FG H  LVVS WE+VK CFTTNDK FS+R     IK MFYD  ++ +AP+G 
Sbjct: 78   YGPVFNIRFGSHKTLVVSDWEIVKECFTTNDKLFSNRPGTLGIKLMFYDADSVGYAPYGA 137

Query: 1390 YWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTKRKDENAGVLVDMSKWFG 1211
            YWR+LRK+ TL LLSN R+  +KH R SE+++C + LY      +       V M  W G
Sbjct: 138  YWRDLRKISTLKLLSNHRIDTIKHLRSSEVESCFESLYSQWGNGEKSGEFAPVRMDSWLG 197

Query: 1210 DISFNVVTRIVAGKHIFGPK----TERYMNVMEEARRLMDVMVFSDVIPYLGWLDRLRGV 1043
            D++FNVV RIVAGK  F        +RY   M+EA RLM    FSDVIP L WLD LRG+
Sbjct: 198  DLTFNVVARIVAGKKNFSANGDVGAQRYKAAMDEAMRLMRFFAFSDVIPSLSWLDNLRGL 257

Query: 1042 DSEIKRTAKELDSVLESWVDEHRQKRVSISAGIGGIVNXXXXXXXXXXXIMLSIIAKNKL 863
              E+K+ A E+DS++ +WV+EHR KR S     GG              + L I+  + L
Sbjct: 258  VREMKKCASEIDSIMATWVEEHRVKRNS-----GG----NSQLEHDFIDVCLDIMEHSSL 308

Query: 862  LGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVE 683
             GDDP  ++K+   +M L   D TTVTLTWA+ LLLN+ QVL++A+ EL+ QVGK RQV+
Sbjct: 309  PGDDPDLVVKSTCLDMILGGSDTTTVTLTWAMSLLLNHPQVLQKAKEELETQVGKNRQVD 368

Query: 682  DSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTRLWINLWKLQRDPN 503
            DSDI  LP+IQAI+KE+MRLYP GP+IER T EDC+V  +++PAGTRL +N+WK+QRD N
Sbjct: 369  DSDIPNLPFIQAIIKETMRLYPAGPLIERRTMEDCEVAGYQVPAGTRLLVNVWKMQRDGN 428

Query: 502  VWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSLQVMHLVLARIIHG 323
            V+  DP EF+P+RFL  +AD+D+KGQH+ELIPFG+GRR+CPGVSF++Q+MHLVLAR++H 
Sbjct: 429  VYKGDPLEFRPDRFLTSNADVDLKGQHYELIPFGAGRRICPGVSFAVQLMHLVLARLLHE 488

Query: 322  FELKT-PTDADIDMSTTLGMISWKATPLEVLLTPR 221
            FE+ T   +  +DM+ + G++ +K  PLEVL+ PR
Sbjct: 489  FEITTVEPETKVDMAESGGLLCYKIMPLEVLIKPR 523


>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  486 bits (1251), Expect = e-135
 Identities = 246/520 (47%), Positives = 351/520 (67%), Gaps = 10/520 (1%)
 Frame = -2

Query: 1750 PIIVSFIVILLFYF--FKKRSSNMIRTKKAPEVVGAWPVIGHLNLLSVPKPAYIVLGELA 1577
            PI V+ I+  +F +  F  R+    + + AP   GAWPV+GHL+L    +  + +L  +A
Sbjct: 11   PISVASIIAFVFLYNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSGELPHKMLAAMA 70

Query: 1576 DQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAIKYMFYDQKTISFAPH 1397
            D+YG AF ++FG H  LVVS   +VK CFTTND  FS+R   KA + M YD ++++F P+
Sbjct: 71   DKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMTYDNESVAFTPY 130

Query: 1396 GPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTKRKDENAGVLVDMSKW 1217
            G YWRE+RK+ TL LLSN RL+ +K  R SE++ C K LY+   K    +A +L+DM KW
Sbjct: 131  GSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYD-QCKNPSGSAPILIDMKKW 189

Query: 1216 FGDISFNVVTRIVAGKHIFGPKTER-------YMNVMEEARRLMDVMVFSDVIPYLGWLD 1058
            F ++S NVV R++ G+  FG K  +       Y  VM+E  RL  + +FSD  P LG++D
Sbjct: 190  FEEVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFAPLLGFVD 249

Query: 1057 RLRGVDSEIKRTAKELDSVLESWVDEHRQKRVSISAGIGGIVNXXXXXXXXXXXIMLSII 878
              +G  S +KR AK++D++LE+W++EHR+K+ S++      ++            MLSI+
Sbjct: 250  IFQGNLSAMKRNAKKVDAILENWLEEHRKKKNSVAESQQDFMDV-----------MLSIV 298

Query: 877  AKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGK 698
             ++KL G D   +IKA    M +   D T V+LTW + LL+NN+  LK+A+ ELDA VGK
Sbjct: 299  EESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKAREELDALVGK 358

Query: 697  ERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTRLWINLWKL 518
            +RQVEDSD+  L Y+ AIVKE+MR+YP G ++ERET EDC++  F +  GTRL +N+WKL
Sbjct: 359  DRQVEDSDLKNLVYMNAIVKETMRMYPLGTLLERETKEDCEIDGFHVKGGTRLLVNVWKL 418

Query: 517  QRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSLQVMHLVLA 338
            QRDPNVW  DP EF+PERFL  +ADID+ GQHFEL+PFG+GRR+CPGV F+LQ MHLVLA
Sbjct: 419  QRDPNVWV-DPTEFRPERFLTENADIDVGGQHFELLPFGAGRRVCPGVXFALQFMHLVLA 477

Query: 337  RIIHGFELKTPTDADIDMS-TTLGMISWKATPLEVLLTPR 221
            R+IHG++L T  + ++D++ +  G ++ KA+PL+++LTPR
Sbjct: 478  RLIHGYDLNTLNEENVDLTESPEGHVNHKASPLDLILTPR 517


>ref|XP_002284806.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
          Length = 527

 Score =  485 bits (1248), Expect = e-134
 Identities = 247/521 (47%), Positives = 347/521 (66%), Gaps = 6/521 (1%)
 Frame = -2

Query: 1747 IIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNLLSVPKPAYIVLGELADQY 1568
            ++++ I +LL   F  +  N  + K+  E  G WP+IGHL+LL   K  +  LG++AD+Y
Sbjct: 15   LLLATICLLLATVFNAKG-NKKKGKRPQEPSGRWPIIGHLHLLGADKLPHRTLGDMADKY 73

Query: 1567 GPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAIKYMFYDQKTISFAPHGPY 1388
            GP F I  G+   LVVSSWE+ K C+TTNDK F++R  + A+K M YD   + FAP+GPY
Sbjct: 74   GPIFCIHLGLRKALVVSSWEVAKECYTTNDKVFATRPRSLAVKLMGYDHAMLGFAPYGPY 133

Query: 1387 WRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTKRKDENAGVLVDMSKWFGD 1208
            WR++RK+  + LLSN +L+MLKH + SE++  +K+LY    + KD  A  LV+M + FG+
Sbjct: 134  WRDVRKLAMVELLSNRQLEMLKHVQDSEVEFLIKELYGQWARNKDSPA--LVEMKERFGN 191

Query: 1207 ISFNVVTRIVAGKHIFG-----PKTERYMNVMEEARRLMDVMVFSDVIPYLGWLDRLRGV 1043
            +  NV+ R +AGK  FG      + +R    +++   L+ + + SD IP+LGWLD ++G 
Sbjct: 192  LVMNVMVRAIAGKRYFGTHACDDEPKRGKKALDDFMLLVGLFMVSDAIPFLGWLDTVKGY 251

Query: 1042 DSEIKRTAKELDSVLESWVDEHRQKRVSISAGIGGIVNXXXXXXXXXXXIMLSIIAKNKL 863
             +++K+ AKELD +L  WV+EHRQ+R+S +     +             +MLS+I   + 
Sbjct: 252  TTDMKKIAKELDYLLGRWVEEHRQQRLSANNNRAEV---------DFLHVMLSVIDDGQF 302

Query: 862  LGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCELDAQVGKERQVE 683
             G DP T+IKA    + LA +D T++TLTWAL LLLNN+  LK+AQ EL+  VGK RQV+
Sbjct: 303  SGRDPDTVIKATCLNLILAGYDTTSITLTWALSLLLNNRHALKKAQAELEIHVGKHRQVD 362

Query: 682  DSDINTLPYIQAIVKESMRLYPPGPI-IERETTEDCDVGDFRIPAGTRLWINLWKLQRDP 506
             SDI  L Y+QAIVKE++RLYPPGP+ +  E  EDC V  F I AGTRL +NLWKL RDP
Sbjct: 363  GSDIKNLVYLQAIVKETLRLYPPGPLSVPHEAMEDCTVAGFHIQAGTRLLVNLWKLHRDP 422

Query: 505  NVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSLQVMHLVLARIIH 326
             VW  DP EFQPERFL  HA +D++G+++EL+PFGSGRR+CPG+SF+L++ HL LAR++H
Sbjct: 423  RVW-LDPLEFQPERFLTNHAGLDVRGKNYELLPFGSGRRVCPGISFALELTHLALARLLH 481

Query: 325  GFELKTPTDADIDMSTTLGMISWKATPLEVLLTPRFPPVFY 203
            GFEL    D+ +DM+   G+ + KATPLEV + PR P   Y
Sbjct: 482  GFELGVVADSPVDMTEGPGLSAPKATPLEVTIVPRLPFELY 522


>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  483 bits (1242), Expect = e-133
 Identities = 249/543 (45%), Positives = 345/543 (63%), Gaps = 15/543 (2%)
 Frame = -2

Query: 1786 LKQWIRTSTLPAPIIVSFIVILLFYFFKKRSSNMIRTK------KAPEVVGAWPVIGHLN 1625
            L  WI   ++ A  +++ +++      K  SS     K      + P V GAWPV GHL+
Sbjct: 11   LNTWISAYSMAA--LLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAVTGAWPVFGHLH 68

Query: 1624 LLSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKA 1445
            L    +  + +L +LA++YGP+F+++FG H  LVVS   +VK CFTTND  FS+R    A
Sbjct: 69   LFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTNDTLFSNRPSTIA 128

Query: 1444 IKYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELST 1265
               M Y   +I+F P+ PYWRELRK+ TL LLSN RL+ +K  R SE+  C K+LY+L+ 
Sbjct: 129  FDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVSVCFKELYDLTN 188

Query: 1264 KRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPK--------TERYMNVMEEARRL 1109
            K+ D  A V +D+ +WF ++S NV+ R++ GK  FG K           Y  +M+E  RL
Sbjct: 189  KKNDNGAPVPIDLKRWFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHYKKIMDELSRL 248

Query: 1108 MDVMVFSDVIPYLGWLDRLRGVDSEIKRTAKELDSVLESWVDEHRQKRVSISAGIGGIVN 929
              + + SD++P LGWLD  +G    +KR  KEL+S+L+ W++EH+ K+ S          
Sbjct: 249  SSLTMLSDMVPLLGWLDYFKGDLRAMKRNGKELNSILQKWLEEHKSKKSS---------- 298

Query: 928  XXXXXXXXXXXIMLSIIAKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNN 749
                       +MLSI    +L G D  T IKA    M +   ++T V LTW L LL+NN
Sbjct: 299  ---DARQDFMDVMLSISKDTQLYGHDQDTFIKATCLAMIMGGTNSTEVALTWILSLLMNN 355

Query: 748  KQVLKRAQCELDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVG 569
            +  L +A+ E+D  VGK+RQVEDSD+  L Y+ AI+KE+MRLYP G ++ER+T EDC+V 
Sbjct: 356  RCALHKAREEIDLLVGKDRQVEDSDVKNLTYMNAIIKETMRLYPLGFLLERDTKEDCEVS 415

Query: 568  DFRIPAGTRLWINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRR 389
             F I  GTRL IN+WKLQRDPNVW  DP EF+PERFL  +ADID+ GQHFEL+PFG+GRR
Sbjct: 416  GFNIKGGTRLLINVWKLQRDPNVW-TDPMEFKPERFLTENADIDVGGQHFELLPFGAGRR 474

Query: 388  MCPGVSFSLQVMHLVLARIIHGFELKTPTDADIDMSTTL-GMISWKATPLEVLLTPRFPP 212
            +CPGVSF+LQ MHLVLAR+IHG++++T    D+D+S +  G ++ K+TPLE++LTPR  P
Sbjct: 475  VCPGVSFALQFMHLVLARLIHGYDMETLNGEDVDLSVSSGGHVNIKSTPLELILTPRLHP 534

Query: 211  VFY 203
              Y
Sbjct: 535  ELY 537