BLASTX nr result
ID: Coptis21_contig00006719
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00006719
(1682 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 485 e-134
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 485 e-134
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 484 e-134
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 483 e-134
ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222... 481 e-133
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 485 bits (1249), Expect = e-134
Identities = 236/452 (52%), Positives = 321/452 (71%), Gaps = 1/452 (0%)
Frame = -1
Query: 1472 IIGNILSLGVKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1293
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 486 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 545
Query: 1292 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLEEMVR 1113
VPH + K+ E N MS+ WA EC + WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 546 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 605
Query: 1112 YLGTKEGKEVNIVEVAFATIINVISGLCFTKDFVQLDDEKEVGGMKQHIRTIMEVGSVPN 933
++ TKEG+ + + EV FAT++N ++ + ++DF+ +D+ + GGMK +R +M + PN
Sbjct: 606 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 665
Query: 932 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 753
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 666 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 724
Query: 752 FSDDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREIYSVFVRDSDL 573
+SDD+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI + SDL
Sbjct: 725 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 784
Query: 572 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 396
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 785 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 844
Query: 395 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGFDWYLP 216
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H FDW LP
Sbjct: 845 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 904
Query: 215 GNICAPELDMNEKFGITLLKEQPLVLIPKMRK 120
ELDMN+KFGI L KEQPL++IPK+RK
Sbjct: 905 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 485 bits (1249), Expect = e-134
Identities = 236/452 (52%), Positives = 321/452 (71%), Gaps = 1/452 (0%)
Frame = -1
Query: 1472 IIGNILSLGVKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1293
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 1292 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLEEMVR 1113
VPH + K+ E N MS+ WA EC + WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 113 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 1112 YLGTKEGKEVNIVEVAFATIINVISGLCFTKDFVQLDDEKEVGGMKQHIRTIMEVGSVPN 933
++ TKEG+ + + EV FAT++N ++ + ++DF+ +D+ + GGMK +R +M + PN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 232
Query: 932 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 753
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 291
Query: 752 FSDDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREIYSVFVRDSDL 573
+SDD+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI + SDL
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 572 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 396
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 395 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGFDWYLP 216
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H FDW LP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 215 GNICAPELDMNEKFGITLLKEQPLVLIPKMRK 120
ELDMN+KFGI L KEQPL++IPK+RK
Sbjct: 472 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 484 bits (1245), Expect = e-134
Identities = 235/452 (51%), Positives = 321/452 (71%), Gaps = 1/452 (0%)
Frame = -1
Query: 1472 IIGNILSLGVKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1293
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 1292 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLEEMVR 1113
VPH + K+ E N MS+ WA EC WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 113 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 1112 YLGTKEGKEVNIVEVAFATIINVISGLCFTKDFVQLDDEKEVGGMKQHIRTIMEVGSVPN 933
++ TKEG+ + + EV FAT++N ++ + ++DF+ +D+ + GGMK +R ++ + PN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 232
Query: 932 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 753
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 291
Query: 752 FSDDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREIYSVFVRDSDL 573
+SDD+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI + SDL
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 572 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 396
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 395 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGFDWYLP 216
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H FDW LP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 215 GNICAPELDMNEKFGITLLKEQPLVLIPKMRK 120
ELDMN+KFG+TL KEQPL++IPK+RK
Sbjct: 472 NGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 483 bits (1244), Expect = e-134
Identities = 235/453 (51%), Positives = 321/453 (70%), Gaps = 1/453 (0%)
Frame = -1
Query: 1472 IIGNILSLGVKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1293
I+GN+L LG PHIS A+ +Q +GPLI+L+LGSQI+++ S+ AA EILKTHDRV S R+
Sbjct: 617 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 676
Query: 1292 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLEEMVR 1113
VPH + K+ E N MS+ WA EC WK LRT+CR ELFS+K +ESQA + E K+ EMVR
Sbjct: 677 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 736
Query: 1112 YLGTKEGKEVNIVEVAFATIINVISGLCFTKDFVQLDDEKEVGGMKQHIRTIMEVGSVPN 933
++ TKEG+ + + EV FAT++N ++ + ++DF+ +D+ + GGMK +R ++ + PN
Sbjct: 737 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 796
Query: 932 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 753
+D++ I +GLD+QGL+KK E + + + W+ +I+ER+E + + S +D L++L+ +
Sbjct: 797 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPS-KQDFLNILIRSG 855
Query: 752 FSDDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREIYSVFVRDSDL 573
+SDD+IN +L AG+D++SST+EW MAEL+K+PE M +V EELAREI + SDL
Sbjct: 856 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 915
Query: 572 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 396
P L YL ACVKET RLHP A L+P RA +C++MNYTIPK S + VN WA+GRDP WE
Sbjct: 916 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 975
Query: 395 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGFDWYLP 216
DPL F P RFL+S +DFKGN+ E IPF AGRRICPG AS+ H FDW LP
Sbjct: 976 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 1035
Query: 215 GNICAPELDMNEKFGITLLKEQPLVLIPKMRKQ 117
ELDMN+KFG+TL KEQPL++IPK RK+
Sbjct: 1036 NGTTPDELDMNDKFGVTLQKEQPLLIIPKKRKK 1068
>ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222859506|gb|EEE97053.1|
cytochrome P450 [Populus trichocarpa]
Length = 503
Score = 481 bits (1238), Expect = e-133
Identities = 236/451 (52%), Positives = 315/451 (69%), Gaps = 1/451 (0%)
Frame = -1
Query: 1472 IIGNILSLGVKPHISFAKLAQVHGPLITLQLGSQIMIIGSSPKAAREILKTHDRVCSARH 1293
IIGN+L +G +PH+S A++A++HGPLI+L+LG+Q++++GSS KAA EILKTHDR SARH
Sbjct: 53 IIGNLLHVGNQPHVSLAEIAKIHGPLISLRLGTQLLVVGSSAKAAAEILKTHDRFLSARH 112
Query: 1292 VPHTLHTKSPESNHMSIVWASECTEKWKYLRTICRTELFSSKAIESQANLREDKLEEMVR 1113
VP + +S +++VW E + WK LR +CRTELFS+KAIES A LRE K+ E++
Sbjct: 113 VPQVIPRESHVLRRVALVWCPESIDTWKLLRGLCRTELFSAKAIESSATLREKKVGELMD 172
Query: 1112 YLGTKEGKEVNIVEVAFATIINVISGLCFTKDFVQLDDEKEVGGMKQHIRTIMEVGSVPN 933
+L +EGK V+I EV F+T+ N IS L F+ D L+++ G+K H+R +M + + PN
Sbjct: 173 FLVAREGKVVSIGEVVFSTVFNTISNLLFSNDLAGLEEKGMSSGLKSHVRKLMLLVATPN 232
Query: 932 FEDYFPILNGLDVQGLHKKFLECVRSMFASWDVLIKERKEAENSEKSTPRDVLDVLLANR 753
D++PI GLD QGL +K + V FA W + IKER+ + + S RD LDV LAN
Sbjct: 233 IADFYPIFAGLDPQGLRRKLSKLVEETFAIWAINIKERRNSYVHD-SPKRDFLDVFLANG 291
Query: 752 FSDDEINHFIFDLFVAGSDSTSSTVEWLMAELMKNPEVMGRVREELAREIYSVFVRDSDL 573
F DD+IN +LF AG+D+T++T+EW +AE++KN EVM +V EEL REI + +SD+
Sbjct: 292 FDDDQINWLAAELFSAGTDTTATTIEWAVAEILKNKEVMKKVDEELEREITKNTISESDV 351
Query: 572 PRLSYLNACVKETWRLHPPAS-LVPHRALETCKIMNYTIPKGSHVCVNLWALGRDPETWE 396
L YLNAC+KET RLHPP LVPHRA ETC++M YTIPK S V VN+WA+ RDP TWE
Sbjct: 352 SGLPYLNACIKETLRLHPPVPLLVPHRATETCEVMKYTIPKDSQVLVNVWAISRDPSTWE 411
Query: 395 DPLTFNPSRFLDSNIDFKGNDFELIPFSAGRRICPGXXXXXXXXXXXXASMVHGFDWYLP 216
DPL+F P RFL SN++FKG ++E +PF AGRRICPG AS++ FDW LP
Sbjct: 412 DPLSFKPDRFLGSNLEFKGGNYEFLPFGAGRRICPGLPMANKLVPLILASLIRCFDWSLP 471
Query: 215 GNICAPELDMNEKFGITLLKEQPLVLIPKMR 123
+LDM +KFG+ L KEQPLVL+PK R
Sbjct: 472 NGEDLAKLDMKDKFGVVLQKEQPLVLVPKRR 502