BLASTX nr result
ID: Coptis21_contig00006652
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00006652
(1241 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hy... 389 e-106
ref|NP_001241587.1| uncharacterized protein LOC100810230 [Glycin... 389 e-106
ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hy... 385 e-104
sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nit... 385 e-104
sp|Q42965.1|NRL4A_TOBAC RecName: Full=Bifunctional nitrilase/nit... 384 e-104
>ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A-like
[Glycine max]
Length = 350
Score = 389 bits (1000), Expect = e-106
Identities = 196/290 (67%), Positives = 227/290 (78%)
Frame = -1
Query: 1181 PTVRATVVQASTVFYDTPATLDKAERLIAGAAAYGSQLVVFPEAFVGGYPRFINFDITDA 1002
PTVRATVVQAST+FYDTPATLDKAERL+A AA+YGSQLVVFPEAFVGGYPR F ++
Sbjct: 28 PTVRATVVQASTIFYDTPATLDKAERLLAEAASYGSQLVVFPEAFVGGYPRGSAFGLSIG 87
Query: 1001 NHPIEEYEEFRKYHTAAIDVPGPEVDRLAAICLKYKVHLVMGVVERVGVTLYSTLLFFDS 822
N + EEFRKYH+AAIDVPGPEVDRLAA+ KYKVHLVMGV+ER G TLY T+LFFDS
Sbjct: 88 NRTAKGREEFRKYHSAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDS 147
Query: 821 QGQCLGKNRKLIPTASEHMIWQSGEKATPPVYKTSVGMIGGLACWDNRMPPLRTELYSEG 642
QG LGK+RK++PTA E +IW G+ +T PV++T VG IG CW+NRMP LRT +Y++G
Sbjct: 148 QGHYLGKHRKIMPTALERVIWGFGDGSTIPVFETPVGKIGAAICWENRMPLLRTAMYAKG 207
Query: 641 VEIYCAPTADARDAWKASMTHIAVEGGCFVLSANQFCQRKDYPLRPVNDSRDTNGDLSPD 462
VEIYCAPTAD+RD W+ASMTHIA+EGGCFVLSANQFC+RKDYP P T DL+PD
Sbjct: 208 VEIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFAGTEEDLTPD 267
Query: 461 XXXXXXXXXXXXXXGAVLAGPNCQGESLISADLDLDEITQAKLEYDVVRH 312
GAVLAGPN GE+LISADLDL EI +AK ++DVV H
Sbjct: 268 SVVCAGGSVIISPSGAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGH 317
>ref|NP_001241587.1| uncharacterized protein LOC100810230 [Glycine max]
gi|255636059|gb|ACU18374.1| unknown [Glycine max]
Length = 350
Score = 389 bits (999), Expect = e-106
Identities = 195/290 (67%), Positives = 227/290 (78%)
Frame = -1
Query: 1181 PTVRATVVQASTVFYDTPATLDKAERLIAGAAAYGSQLVVFPEAFVGGYPRFINFDITDA 1002
PTVRATVVQAST+FYDTPATLDKAERL+A A +YGSQLVVFPEAFVGGYPR F ++
Sbjct: 28 PTVRATVVQASTIFYDTPATLDKAERLLAEATSYGSQLVVFPEAFVGGYPRGSAFGLSIG 87
Query: 1001 NHPIEEYEEFRKYHTAAIDVPGPEVDRLAAICLKYKVHLVMGVVERVGVTLYSTLLFFDS 822
N ++ EEFRKYH+AAIDVPGPEVDRLAA+ KYKVHLVMGV+ER G TLY T+LFFDS
Sbjct: 88 NRTVKGREEFRKYHSAAIDVPGPEVDRLAAMAGKYKVHLVMGVIERDGYTLYCTVLFFDS 147
Query: 821 QGQCLGKNRKLIPTASEHMIWQSGEKATPPVYKTSVGMIGGLACWDNRMPPLRTELYSEG 642
QG LGK+RK++PTA E +IW G+ +T PV++T VG IG CW+NRMP LRT +Y++G
Sbjct: 148 QGHYLGKHRKIMPTALERVIWGFGDGSTIPVFETPVGKIGAAICWENRMPLLRTAMYAKG 207
Query: 641 VEIYCAPTADARDAWKASMTHIAVEGGCFVLSANQFCQRKDYPLRPVNDSRDTNGDLSPD 462
VEIYCAPTADARD W+ASMTHIA+EGGCFVLSANQFC+R+DYP P T DL+PD
Sbjct: 208 VEIYCAPTADARDVWQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFAGTEVDLTPD 267
Query: 461 XXXXXXXXXXXXXXGAVLAGPNCQGESLISADLDLDEITQAKLEYDVVRH 312
GAVLAGPN GE+LISADLDL EI +AK ++DVV H
Sbjct: 268 SVVCAGGSVIISPLGAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGH 317
>ref|XP_002272837.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4B-like [Vitis
vinifera]
Length = 347
Score = 385 bits (989), Expect = e-104
Identities = 195/301 (64%), Positives = 230/301 (76%)
Frame = -1
Query: 1214 AFVDDQCRGPRPTVRATVVQASTVFYDTPATLDKAERLIAGAAAYGSQLVVFPEAFVGGY 1035
A VD PTVRATVVQASTVFYDTPATLDKAERL+A A++YGSQLVVFPEAF+GGY
Sbjct: 15 AEVDMGADSSAPTVRATVVQASTVFYDTPATLDKAERLLAEASSYGSQLVVFPEAFIGGY 74
Query: 1034 PRFINFDITDANHPIEEYEEFRKYHTAAIDVPGPEVDRLAAICLKYKVHLVMGVVERVGV 855
PR NF +T N + E+FRKYH AAIDVPGPEVDRLAA+ KYKV+LVMGV+ER G
Sbjct: 75 PRGSNFGVTIGNRTAKGREDFRKYHAAAIDVPGPEVDRLAAMAGKYKVYLVMGVIERDGY 134
Query: 854 TLYSTLLFFDSQGQCLGKNRKLIPTASEHMIWQSGEKATPPVYKTSVGMIGGLACWDNRM 675
TLY T+LFFDSQG LGK+RK++PTA E +IW G+ +T PVY+T +G IG CW+NRM
Sbjct: 135 TLYCTVLFFDSQGHYLGKHRKVMPTALERIIWGFGDGSTIPVYETPIGKIGAAICWENRM 194
Query: 674 PPLRTELYSEGVEIYCAPTADARDAWKASMTHIAVEGGCFVLSANQFCQRKDYPLRPVND 495
P LRT +Y++G+EIYCAPTADARD W++SMTHIA+EGGCFVLSANQFC+RKDYP P +
Sbjct: 195 PLLRTAMYAKGIEIYCAPTADARDIWQSSMTHIALEGGCFVLSANQFCRRKDYPPPPEYE 254
Query: 494 SRDTNGDLSPDXXXXXXXXXXXXXXGAVLAGPNCQGESLISADLDLDEITQAKLEYDVVR 315
+ DL+PD G VLAGPN GE+LISADLDL EI +AK ++DVV
Sbjct: 255 FSGAD-DLTPDSVVCAGGSVIISPSGTVLAGPNYDGEALISADLDLGEIARAKFDFDVVG 313
Query: 314 H 312
H
Sbjct: 314 H 314
>sp|Q42966.1|NRL4B_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4B;
Short=TNIT4B; AltName: Full=Cyanoalanine nitrilase B;
AltName: Full=Nitrilase 4B gi|1181615|dbj|BAA11770.1|
nitrilase [Nicotiana tabacum]
Length = 348
Score = 385 bits (988), Expect = e-104
Identities = 202/322 (62%), Positives = 238/322 (73%), Gaps = 5/322 (1%)
Frame = -1
Query: 1214 AFVDDQCRGPRPTVRATVVQASTVFYDTPATLDKAERLIAGAAAYGSQLVVFPEAFVGGY 1035
A VD PTVRATVVQAST+FYDTPATLDKAERL+A AA+YG+QLVVFPEAF+GGY
Sbjct: 16 AEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASYGAQLVVFPEAFIGGY 75
Query: 1034 PRFINFDITDANHPIEEYEEFRKYHTAAIDVPGPEVDRLAAICLKYKVHLVMGVVERVGV 855
PR F ++ N + EEFRKYH +AIDVPGPEVDRLAA+ KYKV+LVMGV+ER G
Sbjct: 76 PRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLVMGVIERDGY 135
Query: 854 TLYSTLLFFDSQGQCLGKNRKLIPTASEHMIWQSGEKATPPVYKTSVGMIGGLACWDNRM 675
TLY T+LFFDSQG LGK+RK++PTA E +IW G+ +T PVY T +G IG CW+NRM
Sbjct: 136 TLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAAICWENRM 195
Query: 674 PPLRTELYSEGVEIYCAPTADARDAWKASMTHIAVEGGCFVLSANQFCQRKDYPLRPVND 495
P LRT +Y++G+EIYCAPTAD+RD W+ASMTHIA+EGGCFVLSANQFC+RKDYP P
Sbjct: 196 PLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYV 255
Query: 494 SRDTNGDLSPDXXXXXXXXXXXXXXGAVLAGPNCQGESLISADLDLDEITQAKLEYDVVR 315
T DL+PD GAVLAGPN +GE+LISADLDL EI +AK ++DVV
Sbjct: 256 FSGTE-DLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLDLGEIARAKFDFDVVG 314
Query: 314 HAA-----SLGLNRKLVSSVSF 264
H A SL + VS VSF
Sbjct: 315 HYARPEVLSLIVRDHAVSPVSF 336
>sp|Q42965.1|NRL4A_TOBAC RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4A;
Short=TNIT4A; AltName: Full=Cyanoalanine nitrilase A;
AltName: Full=Nitrilase 4A gi|1171482|dbj|BAA09645.1|
nitrilase [Nicotiana tabacum]
Length = 349
Score = 384 bits (985), Expect = e-104
Identities = 201/322 (62%), Positives = 236/322 (73%), Gaps = 5/322 (1%)
Frame = -1
Query: 1214 AFVDDQCRGPRPTVRATVVQASTVFYDTPATLDKAERLIAGAAAYGSQLVVFPEAFVGGY 1035
A VD PTVRATVVQAST+FYDTPATL KAERL+A AA+YG+QLVVFPEAF+GGY
Sbjct: 16 AEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVVFPEAFIGGY 75
Query: 1034 PRFINFDITDANHPIEEYEEFRKYHTAAIDVPGPEVDRLAAICLKYKVHLVMGVVERVGV 855
PR F ++ N + EEFRKYH +AIDVPGPEVDRLAA+ KYKV+LVMGV+ER G
Sbjct: 76 PRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLVMGVIERDGY 135
Query: 854 TLYSTLLFFDSQGQCLGKNRKLIPTASEHMIWQSGEKATPPVYKTSVGMIGGLACWDNRM 675
TLY T+LFFDSQG LGK+RK++PTA E +IW G+ +T PVY T +G IG CW+NRM
Sbjct: 136 TLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAAICWENRM 195
Query: 674 PPLRTELYSEGVEIYCAPTADARDAWKASMTHIAVEGGCFVLSANQFCQRKDYPLRPVND 495
P LRT +Y++G+EIYCAPTAD+RD W+ASMTHIA+EGGCFVLSANQFC+RKDYP P
Sbjct: 196 PLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYV 255
Query: 494 SRDTNGDLSPDXXXXXXXXXXXXXXGAVLAGPNCQGESLISADLDLDEITQAKLEYDVVR 315
T DL+PD GAVLAGPN GE+LISADLDL EI +AK ++DVV
Sbjct: 256 FSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLDLGEIARAKFDFDVVG 315
Query: 314 HAA-----SLGLNRKLVSSVSF 264
H A SL + VS VSF
Sbjct: 316 HYARPEVLSLIVRDHAVSPVSF 337