BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00006579
         (2013 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   486   e-134
emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]   484   e-134
gb|ABC69377.1| CYP81B2v2 [Nicotiana tabacum]                          481   e-133
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   475   e-131
gb|ABC69378.1| CYP81B2v1 [Nicotiana tabacum] gi|158635852|dbj|BA...   475   e-131

>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  486 bits (1250), Expect = e-134
 Identities = 250/503 (49%), Positives = 338/503 (67%), Gaps = 2/503 (0%)
 Frame = +3

Query: 381  YILIF-FILFITKYLFQKLTNQNXXXXXXXXXXXXXXXXXXXXXXXXXQSLSLKHGSIMF 557
            YIL+F FI F+TK+ FQ    +                            +S ++G I+F
Sbjct: 7    YILLFLFIYFLTKHFFQS-NKKLPPSPPLSLPIIGHLHLFKKPLHRTFAKISNQYGPILF 65

Query: 558  LKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQKYTALGWVPYGEHWRNLR 737
            ++FG R V+ VSSP+  EE FT NDIVFANRP L+AG+HLG  YT L W PYG+HWRNLR
Sbjct: 66   IRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYTTLTWAPYGQHWRNLR 125

Query: 738  RVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLFDLTVNIMMRI 917
            R+ S+EI              +E++  + ++F  +   ++  +D++SM F+LT+N MMR+
Sbjct: 126  RIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDMKSMFFELTLNNMMRM 185

Query: 918  VVGRPCYGENMG-IEEKSRMREFLKETFVPNMAMNLGDIYPVFRRFFSFVGLDKKLVKLQ 1094
            + G+  YG+N+  +EE  + RE + ETF  + A N+ D  P F ++    G++KKLV LQ
Sbjct: 186  ISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVDFVP-FSKWIGLNGIEKKLVILQ 244

Query: 1095 STREEFLQGLIEETRIKRVDDSIDGEKNRTTVTDGLLSLQKMEPDYYSDDVIRGIISIMF 1274
              R+ F+Q LIEE R  R+    +G     T+ D LLSLQ+ EP+ Y+DD+IRG++ +M 
Sbjct: 245  GKRDGFMQNLIEEHR--RMRSPCEGRSK--TMLDVLLSLQETEPECYTDDIIRGMMQVML 300

Query: 1275 TAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGCLIDDSDLANLPYLHCILNET 1454
            +AGTDTS+ TMEWAMSLLLN+PE LEKA+AEIDSH+    LID+ D+A LPYL  I+ ET
Sbjct: 301  SAGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSRLIDELDIAELPYLRGIIKET 360

Query: 1455 LRLYPAAPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPNNFKPERFTGV 1634
            LR+YPAAPLLVPH SSEECT+GG+++P GTMLLVN +AI  DP LW EP+ FKPERF G 
Sbjct: 361  LRMYPAAPLLVPHESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPSKFKPERFQGP 420

Query: 1635 QGEREGFKFFPFGMGRRVCIGAGMGVKSVSLVVGTLIQCFEWKRLGPELVDLTEGSGTTM 1814
            +G+R GF F PFG GRR C G G+ ++ V L +G+LIQ FEW+R+  E+VD++EG+G TM
Sbjct: 421  EGQRNGFMFSPFGAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMVDMSEGTGLTM 480

Query: 1815 PKAESLEATYRPRPQLMQFLSQL 1883
            PKA+SL A  RPRP ++  LSQL
Sbjct: 481  PKAQSLVAKCRPRPSMVSLLSQL 503


>emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]
          Length = 1001

 Score =  484 bits (1245), Expect = e-134
 Identities = 250/503 (49%), Positives = 336/503 (66%), Gaps = 2/503 (0%)
 Frame = +3

Query: 381  YILIF-FILFITKYLFQKLTNQNXXXXXXXXXXXXXXXXXXXXXXXXXQSLSLKHGSIMF 557
            YIL+F FI F+TK+ FQ    +                            +S ++G I+F
Sbjct: 505  YILLFLFIYFLTKHFFQS-NKKLPPSPPLSLPIIGHLHLFKKPLHRTFAKISNQYGPILF 563

Query: 558  LKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQKYTALGWVPYGEHWRNLR 737
            ++FG R V+ VSSP+  EE FT NDIVFANRP L+AG+HLG  YT L W PYG+HWRNLR
Sbjct: 564  IRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYTTLTWAPYGQHWRNLR 623

Query: 738  RVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLFDLTVNIMMRI 917
            R+ S+EI              +E++  + ++F  +   ++  +D++SM F+LT+N MMR+
Sbjct: 624  RIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDMKSMFFELTLNNMMRM 683

Query: 918  VVGRPCYGENMG-IEEKSRMREFLKETFVPNMAMNLGDIYPVFRRFFSFVGLDKKLVKLQ 1094
            + G+  YG+N+  +EE  + RE + ETF  + A N+ D  P F ++    G++KKLV LQ
Sbjct: 684  ISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVDFVP-FSKWIGLNGIEKKLVILQ 742

Query: 1095 STREEFLQGLIEETRIKRVDDSIDGEKNRTTVTDGLLSLQKMEPDYYSDDVIRGIISIMF 1274
              R+ F+Q LIEE R  R       E    T+ D LLSLQ+ EP+ Y+DD+IRG++ +M 
Sbjct: 743  GKRDGFMQNLIEEHRRMRSPC----EXRSKTMLDVLLSLQETEPECYTDDIIRGMMQVML 798

Query: 1275 TAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGCLIDDSDLANLPYLHCILNET 1454
            +AGTDTS+ TMEWAMSLLLN+PE LEKA+AEIDSH+    LID+ D+A LPYL  I+ ET
Sbjct: 799  SAGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSRLIDELDIAXLPYLRGIIMET 858

Query: 1455 LRLYPAAPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPNNFKPERFTGV 1634
            LR+YPAAPLLVPH SSEECT+GG+++P GTMLLVN +AI  DP LW EP+ FKPERF G 
Sbjct: 859  LRMYPAAPLLVPHESSEECTVGGFRVPSGTMLLVNMWAIQNDPMLWAEPSKFKPERFQGP 918

Query: 1635 QGEREGFKFFPFGMGRRVCIGAGMGVKSVSLVVGTLIQCFEWKRLGPELVDLTEGSGTTM 1814
            +G+R GF F PFG GRR C G G+ ++ V L +G+LIQ FEW+R+  E+VD++EG+G TM
Sbjct: 919  EGQRNGFMFSPFGAGRRGCPGEGLAMRVVGLALGSLIQFFEWERVDEEMVDMSEGTGLTM 978

Query: 1815 PKAESLEATYRPRPQLMQFLSQL 1883
            PKA+SL A  RPRP ++  LSQL
Sbjct: 979  PKAQSLVAKCRPRPSMVSLLSQL 1001


>gb|ABC69377.1| CYP81B2v2 [Nicotiana tabacum]
          Length = 511

 Score =  481 bits (1237), Expect = e-133
 Identities = 243/507 (47%), Positives = 336/507 (66%), Gaps = 7/507 (1%)
 Frame = +3

Query: 384  ILIFFILFITKYLFQKLTNQNXXXXXXXXXXXXXXXXXXXXXXXXXQSLSLKHGSIMFLK 563
            +L F+I  ITK+  +KL N N                           +S ++GS++ L+
Sbjct: 14   LLAFYI--ITKHFLRKLRN-NPPAPFLTFPFIGHLYLFKKPLQRTLAKISERYGSVLLLE 70

Query: 564  FGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQKYTALGWVPYGEHWRNLRRV 743
            FG R VL VSSP+  EE  T NDI+FANRP L+AG+HLG  +T+L W  YG+HWRNLRR+
Sbjct: 71   FGSRKVLLVSSPSAAEECLTKNDIIFANRPLLMAGKHLGYNFTSLAWSSYGDHWRNLRRI 130

Query: 744  TSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLFDLTVNIMMRIVV 923
            TS+E+F             +E+K  VK + S    +  K +D++SM F+L +N+MMR + 
Sbjct: 131  TSVEMFSTHRLQMLHGIRIDEVKSMVKRLNSSAMAE--KSVDMKSMFFELMLNVMMRTIA 188

Query: 924  GRPCYGENM-GIEEKSRMREFLKETFVPNMAMNLGDIYPVFRRFFSFVGLDKKLVKLQST 1100
            G+  YGEN+  IEE +R +  ++ETF    A N+GD  P  +       L+K L+ LQ  
Sbjct: 189  GKRYYGENVEDIEEATRFKGLVQETFRIGGATNIGDFLPALKLLVR--KLEKSLIVLQEN 246

Query: 1101 REEFLQGLIEETRIKR------VDDSIDGEKNRTTVTDGLLSLQKMEPDYYSDDVIRGII 1262
            R+EF+Q LI++ R +        D  I+G  N+  + + LL+LQ+ EP+YY D++IR ++
Sbjct: 247  RDEFMQELIKDCRKRMEKEGTVTDSEIEG--NKKCLIEVLLTLQENEPEYYKDEIIRSLM 304

Query: 1263 SIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGCLIDDSDLANLPYLHCI 1442
             ++ +AGTDTS  TMEWA+SL+LNHPE L+KA+AEID HI +  L+D+SD+ NLPYL CI
Sbjct: 305  LVLLSAGTDTSVGTMEWALSLMLNHPETLKKAQAEIDEHIGHERLVDESDINNLPYLRCI 364

Query: 1443 LNETLRLYPAAPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPNNFKPER 1622
            +NET R+YPA PLLVPH SSEE T+GGY++P GTMLLVN +AIH DPKLW+EP  FKPER
Sbjct: 365  INETFRMYPAGPLLVPHESSEETTVGGYRVPGGTMLLVNLWAIHNDPKLWDEPRKFKPER 424

Query: 1623 FTGVQGEREGFKFFPFGMGRRVCIGAGMGVKSVSLVVGTLIQCFEWKRLGPELVDLTEGS 1802
            F G+ G R+G+K  PFG GRR C G G+ V+ V+L +G +IQCF+W+R+G ELVD+TEG+
Sbjct: 425  FQGLDGVRDGYKMMPFGSGRRSCPGEGLAVRMVALSLGCIIQCFDWQRIGEELVDMTEGT 484

Query: 1803 GTTMPKAESLEATYRPRPQLMQFLSQL 1883
            G T+PKA+ L A   PRP++   LSQ+
Sbjct: 485  GLTLPKAQPLVAKCSPRPKMANLLSQI 511


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  475 bits (1223), Expect = e-131
 Identities = 239/508 (47%), Positives = 343/508 (67%), Gaps = 8/508 (1%)
 Frame = +3

Query: 384  ILIFFILFITKYLFQKLT-----NQNXXXXXXXXXXXXXXXXXXXXXXXXXQSLSLKHGS 548
            IL++F+LFIT  L  KL      ++N                          ++S ++G 
Sbjct: 4    ILVYFLLFITFLLLSKLVFSSPKHKNLPPSPPSLPIIGHLHLFKKPLYRTLTNISNQYGP 63

Query: 549  IMFLKFGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQKYTALGWVPYGEHWR 728
            +++ +FG R VL VSSP+  EE  T NDI FANRP L+ G+H G  YT L W  YG++WR
Sbjct: 64   VLYFQFGSRPVLVVSSPSAAEECLTKNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWR 123

Query: 729  NLRRVTSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLFDLTVNIM 908
            NLRR++++E+F             +E+K  V+ + +     E+  ++++S+ F+LT+NIM
Sbjct: 124  NLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLVAGY---EYHKVEMKSVFFELTLNIM 180

Query: 909  MRIVVGRPCYGENMG-IEEKSRMREFLKETFVPNMAMNLGDIYPVFRRFFSFVGLDKKLV 1085
            MR++ G+  YGE++  +E+  + +E ++ETF  + A N+GD  P+ R +  + G++K+L+
Sbjct: 181  MRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLR-WIGYKGIEKRLI 239

Query: 1086 KLQSTREEFLQGLIEETRIKRVDD-SIDGEKNRTTVTDGLLSLQKMEPDYYSDDVIRGII 1262
            +L++ R+EFLQ LI+E R  RV+  S   E+ + T+ D LLSLQ+ EP+YY+D++IRG+I
Sbjct: 240  RLKNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLI 299

Query: 1263 SIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGCLIDDSDLANLPYLHCI 1442
             I+F AGTDTS+ TMEWAMSLLLN+P++++KA++EID  IE G  ID+SDL  LPYLHCI
Sbjct: 300  WILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCI 359

Query: 1443 LNETLRLYPAAPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPNNFKPER 1622
            +NETLR+YPA PLL+PH SSE+C IGGY IP GTMLLVN +AI  DP LW+EP  FKPER
Sbjct: 360  INETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPER 419

Query: 1623 FTGVQ-GEREGFKFFPFGMGRRVCIGAGMGVKSVSLVVGTLIQCFEWKRLGPELVDLTEG 1799
            F G Q G R+GFK  PFG GRR C G G+  + V L + +L+QCF+W R+G E+V+++EG
Sbjct: 420  FEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMSEG 479

Query: 1800 SGTTMPKAESLEATYRPRPQLMQFLSQL 1883
            +G T+PK   LEA  RPR  ++ FLSQ+
Sbjct: 480  TGLTLPKLHPLEAHCRPRSTMLNFLSQV 507


>gb|ABC69378.1| CYP81B2v1 [Nicotiana tabacum] gi|158635852|dbj|BAF91366.1| cytochrome
            P450 [Nicotiana tabacum]
          Length = 511

 Score =  475 bits (1223), Expect = e-131
 Identities = 242/507 (47%), Positives = 334/507 (65%), Gaps = 7/507 (1%)
 Frame = +3

Query: 384  ILIFFILFITKYLFQKLTNQNXXXXXXXXXXXXXXXXXXXXXXXXXQSLSLKHGSIMFLK 563
            +L F+I  ITK+  +KL N N                           +S ++GS++ L+
Sbjct: 14   LLAFYI--ITKHFLRKLRN-NPPAPFLTFPFIGHLYLFKKPLQRTLAKISERYGSVLLLE 70

Query: 564  FGFRNVLTVSSPTYVEEIFTTNDIVFANRPNLVAGQHLGQKYTALGWVPYGEHWRNLRRV 743
            FG R VL VSSP+  EE  T NDI+FANRP L+AG+HLG  +T+L W  YG+HWRNLRR+
Sbjct: 71   FGSRKVLLVSSPSAAEECLTKNDIIFANRPLLMAGKHLGYNFTSLAWSSYGDHWRNLRRI 130

Query: 744  TSIEIFXXXXXXXXXXXXXEEIKRSVKEMFSQTRLDEWKFIDVRSMLFDLTVNIMMRIVV 923
            TS+E+F             +E+K  VK + S    +  K +D++SM F+L +N+MMR + 
Sbjct: 131  TSVEMFSTHRLQMLHGIRIDEVKSMVKRLNSSAIAE--KSVDMKSMFFELMLNVMMRTIA 188

Query: 924  GRPCYGENM-GIEEKSRMREFLKETFVPNMAMNLGDIYPVFRRFFSFVGLDKKLVKLQST 1100
            G+  YGEN+  IEE +R +  ++ETF    A N+GD  P  +       L+K L+ LQ  
Sbjct: 189  GKRYYGENVEDIEEATRFKGLVQETFRIGGATNIGDFLPALKLLVR--KLEKSLIVLQEN 246

Query: 1101 REEFLQGLIEETRIKR------VDDSIDGEKNRTTVTDGLLSLQKMEPDYYSDDVIRGII 1262
            R+EF+Q LI++ R +        D  I+G  N+  + + LL+LQ+ EP+YY D++IR ++
Sbjct: 247  RDEFMQELIKDCRKRMEKEGTVTDSEIEG--NKKCLIEVLLTLQENEPEYYKDEIIRSLM 304

Query: 1263 SIMFTAGTDTSSATMEWAMSLLLNHPEVLEKARAEIDSHIENGCLIDDSDLANLPYLHCI 1442
             ++ +AGTDTS  TMEWA+SL+LNHPE L+KA+AEID HI +  L+D+SD+ NLPYL CI
Sbjct: 305  LVLLSAGTDTSVGTMEWALSLMLNHPETLKKAQAEIDEHIGHERLVDESDINNLPYLRCI 364

Query: 1443 LNETLRLYPAAPLLVPHSSSEECTIGGYKIPRGTMLLVNAYAIHRDPKLWEEPNNFKPER 1622
            +NET R+YPA PLLVPH SSEE T+GGY++P GTMLLVN +AIH DPKLW+EP  FKPER
Sbjct: 365  INETFRMYPAGPLLVPHESSEETTVGGYRVPGGTMLLVNLWAIHNDPKLWDEPRKFKPER 424

Query: 1623 FTGVQGEREGFKFFPFGMGRRVCIGAGMGVKSVSLVVGTLIQCFEWKRLGPELVDLTEGS 1802
            F G++G R+G+K  PFG GRR C G G+ ++ V+L +G +IQCF+W+RLG  LVD TEG+
Sbjct: 425  FEGLEGVRDGYKMMPFGSGRRSCPGEGLAIRMVALSLGCIIQCFDWQRLGEGLVDKTEGT 484

Query: 1803 GTTMPKAESLEATYRPRPQLMQFLSQL 1883
            G T+PKA+ L A   PRP +   LSQ+
Sbjct: 485  GLTLPKAQPLVAKCSPRPIMANLLSQI 511