BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00006488
         (1948 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002533700.1| amsh, putative [Ricinus communis] gi|2235263...   422   e-169
ref|XP_002278560.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   399   e-168
ref|XP_002321479.1| predicted protein [Populus trichocarpa] gi|2...   420   e-162
ref|XP_003525234.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   398   e-155
ref|XP_003528952.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   388   e-154

>ref|XP_002533700.1| amsh, putative [Ricinus communis] gi|223526395|gb|EEF28683.1| amsh,
            putative [Ricinus communis]
          Length = 514

 Score =  422 bits (1084), Expect(2) = e-169
 Identities = 207/300 (69%), Positives = 254/300 (84%), Gaps = 2/300 (0%)
 Frame = +3

Query: 726  YPSNIDLTPIEIPSLQKPVEDNVFVQKDTSS-DLERLTLESVLSLNVDSHSLLTEELCSV 902
            YPSNIDL+P+EIP     +E+ + ++ D+SS +LE L+ +SVL++N +S +   EEL  +
Sbjct: 219  YPSNIDLSPVEIPRFDSSLENGLALKLDSSSSELETLSSQSVLTVNDNSQTSRVEELSPM 278

Query: 903  TTSSLDTGLMELDMQTSIIRQPSPSPVLAEVQDLMPVTSPQVSNSVSEF-ATLPDELARS 1079
               S +T   E  +QT +IRQPSP  VLAEVQDL+P  SPQ + + ++   + PD++ RS
Sbjct: 279  I--SFET--TETPVQTDLIRQPSPPAVLAEVQDLIPAMSPQATEAENKMDISSPDDIVRS 334

Query: 1080 KCPLQLHISTTMMEHFLSLAKSNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSDSC 1259
            + PLQLHISTTMME+F+ LAK+NTD+NLETCG+LAGSLKNRKF++TALIIPKQESTSDSC
Sbjct: 335  ESPLQLHISTTMMENFMKLAKANTDRNLETCGVLAGSLKNRKFYVTALIIPKQESTSDSC 394

Query: 1260 QATNEEEIFDVQDKQSLFQLGWIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPKD 1439
            Q TNEEEIF+VQDK+SLF LGWIHTHP+QSCFMSSID+HTHYSYQIMLPE+VAIVMAP+D
Sbjct: 395  QTTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRD 454

Query: 1440 SSRTHGIFRLTSPGGVSVIRNCDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDLR 1619
            +SRTHGIFRLT+PGG+SVIRNC +RGFHPHD+PPDGGPIY+ CTDVYMNPNLKFDVIDLR
Sbjct: 455  TSRTHGIFRLTTPGGMSVIRNCQQRGFHPHDQPPDGGPIYKTCTDVYMNPNLKFDVIDLR 514



 Score =  202 bits (515), Expect(2) = e-169
 Identities = 106/214 (49%), Positives = 142/214 (66%), Gaps = 4/214 (1%)
 Frame = +1

Query: 40  GGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFRGEENVVDLFVMLMRFSSLITET 219
           G  +IA +  + DVDNRISLR+YYRIADN+L+QA+IFR E+N++DL++ML+RFSSL++ET
Sbjct: 8   GRINIATSAQKIDVDNRISLRFYYRIADNILKQADIFREEKNIIDLYIMLLRFSSLVSET 67

Query: 220 IPCHKDYKLYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKVNRLATTQVDRWGHHQSN 399
           IPCH+DY+   Q +K                  P VQ++ +++NR  T QV+ WG    N
Sbjct: 68  IPCHRDYRTSQQSKKIYLKQKLLNAVKELEQLKPAVQQKINELNRKHTHQVNGWGSVNQN 127

Query: 400 YRDPSLEWPPVKKQLPKYNEIRKTPRYVGSEGT----GSLVQQDKFSLSRPMEEQYRKVN 567
               SLEWPPVKK+     +   T   + +       GS  QQ   S +RP+ EQ+RK++
Sbjct: 128 ---DSLEWPPVKKKTLTGYDAGATKAVIPAAREFVYHGSRTQQ--LSYARPVGEQFRKMS 182

Query: 568 LNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIG 669
           LN P+PKEETLSRHSILGPNGL+GQWQPP S+ G
Sbjct: 183 LNFPQPKEETLSRHSILGPNGLQGQWQPPKSDKG 216


>ref|XP_002278560.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1 [Vitis vinifera]
            gi|297734223|emb|CBI15470.3| unnamed protein product
            [Vitis vinifera]
          Length = 506

 Score =  399 bits (1026), Expect(2) = e-168
 Identities = 203/302 (67%), Positives = 241/302 (79%), Gaps = 3/302 (0%)
 Frame = +3

Query: 723  RYPSNIDLTPIEIPSLQKPVEDNVFVQKD-TSSDLERLTLESVLSLNVDSHSLLTEELCS 899
            +YPSN+DLTP++ PSLQ  VED +    + +SS+ E+ +LESVL+LN D+     EEL S
Sbjct: 211  QYPSNLDLTPVQFPSLQHHVEDGLMNNNEASSSEHEQSSLESVLTLNEDNQIHPAEELGS 270

Query: 900  -VTTSSLDTGLMELDMQTSIIRQPSPSPVLAEVQDLMPVTSPQVSNSVSEFAT-LPDELA 1073
             ++  + +T        T IIRQPSP PVLA+VQDL+P T  QVS    +  T   D L 
Sbjct: 271  LISFETTETP------HTEIIRQPSPPPVLADVQDLIPETPTQVSEVNGQMETPSTDGLV 324

Query: 1074 RSKCPLQLHISTTMMEHFLSLAKSNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSD 1253
             S+ PL++HIST +ME FL LAKSNT +NLETCGILAGSLKNRKF++TALIIPKQESTSD
Sbjct: 325  CSEAPLEMHISTVLMESFLKLAKSNTVRNLETCGILAGSLKNRKFYVTALIIPKQESTSD 384

Query: 1254 SCQATNEEEIFDVQDKQSLFQLGWIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAP 1433
            SCQ TNEEEIF+VQDKQSLF LGWIHTHP+QSCFMSS+DLHTHYSYQ MLPEA+AIVMAP
Sbjct: 385  SCQTTNEEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSVDLHTHYSYQTMLPEAIAIVMAP 444

Query: 1434 KDSSRTHGIFRLTSPGGVSVIRNCDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVID 1613
            KD+S+ HGIFRLT+PGG+SVIR+CD+RGFHPH  P DGGPIY+ CTDVYMNPN+ FDVID
Sbjct: 445  KDASKKHGIFRLTTPGGMSVIRHCDQRGFHPHHSPSDGGPIYKSCTDVYMNPNIHFDVID 504

Query: 1614 LR 1619
            LR
Sbjct: 505  LR 506



 Score =  221 bits (564), Expect(2) = e-168
 Identities = 113/213 (53%), Positives = 151/213 (70%), Gaps = 2/213 (0%)
 Frame = +1

Query: 25  MKTSNGGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFRGEENVVDLFVMLMRFSS 204
           M+ S+GG +IAA+  + DVDNRISLRYYYRIADN+L+QA+IFR E+N++DL++ML+RFSS
Sbjct: 1   MRPSSGGINIAASAQKLDVDNRISLRYYYRIADNILKQADIFREEKNIIDLYIMLLRFSS 60

Query: 205 LITETIPCHKDYKLYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKVNRLATTQVDRWG 384
           L +ETIPCH+DYK   Q +K+                 P V+++ D++N+  T QV+RWG
Sbjct: 61  LTSETIPCHRDYKASLQSKKNYLKKNLLRALCELEELKPAVRQKIDELNQKHTYQVNRWG 120

Query: 385 HHQSNYRDPSLEWPPVKKQLPKYNEIRKTPRYVGS--EGTGSLVQQDKFSLSRPMEEQYR 558
                  D SLEWP V+KQ    +E+ KTPR      E  GS +QQ  FS ++P+EEQ+R
Sbjct: 121 ------ADSSLEWPHVRKQTLTKHEMTKTPRPTARDFEYQGSKIQQ--FSRAKPVEEQFR 172

Query: 559 KVNLNIPRPKEETLSRHSILGPNGLRGQWQPPT 657
           ++++ I RP+EETLSRHSILGPNGL GQWQPPT
Sbjct: 173 RLSVGILRPREETLSRHSILGPNGLHGQWQPPT 205


>ref|XP_002321479.1| predicted protein [Populus trichocarpa] gi|222868475|gb|EEF05606.1|
            predicted protein [Populus trichocarpa]
          Length = 497

 Score =  420 bits (1080), Expect(2) = e-162
 Identities = 212/303 (69%), Positives = 253/303 (83%), Gaps = 4/303 (1%)
 Frame = +3

Query: 723  RYPSNIDLTPIEIPSLQKPVEDNVFVQKD-TSSDLERLTLESVLSLNVDSHSLLTEELCS 899
            +YPS IDLTP+EIP LQ+ V+  +FV+ + +SS+  R +LES+LS+  D+     EE CS
Sbjct: 199  KYPSIIDLTPVEIPRLQESVKAELFVKSEHSSSEPGRSSLESILSVQDDNQKCRDEEPCS 258

Query: 900  VTTSSLDTGLMELDMQTSIIRQPSPSPVLAEVQDLMPVTSPQVSNSVSEF-ATLPDELAR 1076
            +   S +T  +E  +  ++IRQPSP PVLAEVQDL+P T PQVS + ++   + P++L  
Sbjct: 259  LI--SFET--IETPVLPAVIRQPSPPPVLAEVQDLIPATPPQVSEAENKMDISSPNDLIC 314

Query: 1077 SKCPLQLHISTTMMEHFLSLAKSNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSDS 1256
            S+ PLQLHISTT+ME+F+ +AKSNTDKNLETCG+LAGSLKNRKF++TALIIPKQESTSDS
Sbjct: 315  SEAPLQLHISTTLMENFMKMAKSNTDKNLETCGVLAGSLKNRKFYVTALIIPKQESTSDS 374

Query: 1257 CQATNEEEIFDVQDKQSLFQLGWIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPK 1436
            CQ TNEEEIF+VQDKQSLF LGWIHTHP+QSCFMSSID+HTHYSYQIMLPEAVAIVMAP+
Sbjct: 375  CQTTNEEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPR 434

Query: 1437 DSSR--THGIFRLTSPGGVSVIRNCDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVI 1610
            DSSR  THGIFRLT+PGG+SVIR C  RGFHPHD PPDGGPIY+ CTDVYMNPNLKFDVI
Sbjct: 435  DSSRQVTHGIFRLTTPGGMSVIRQCQHRGFHPHDPPPDGGPIYKPCTDVYMNPNLKFDVI 494

Query: 1611 DLR 1619
            DLR
Sbjct: 495  DLR 497



 Score =  180 bits (457), Expect(2) = e-162
 Identities = 95/207 (45%), Positives = 130/207 (62%)
 Frame = +1

Query: 49  SIAANVPRRDVDNRISLRYYYRIADNLLRQANIFRGEENVVDLFVMLMRFSSLITETIPC 228
           +I A   R DVDNR +L  Y+RIA+N+L+QA IFR E+N++DL+VML+RFSSL++ETIPC
Sbjct: 5   NIEARTQRLDVDNRFALNIYFRIANNILKQAEIFRAEKNIIDLYVMLLRFSSLVSETIPC 64

Query: 229 HKDYKLYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKVNRLATTQVDRWGHHQSNYRD 408
           H+ Y  + Q +KD                   VQ+R +++NR  T QV+ WG++  N   
Sbjct: 65  HRGYGAFPQSKKDYLRKKLLNALGELEQLKTAVQQRINELNRKHTHQVNGWGYNSQN--- 121

Query: 409 PSLEWPPVKKQLPKYNEIRKTPRYVGSEGTGSLVQQDKFSLSRPMEEQYRKVNLNIPRPK 588
             LE PP  K++   NE      Y GS       +  ++S  RP+E+Q+R+V+LN  RP 
Sbjct: 122 DLLEKPPYNKKILNGNE----SLYQGS-------RTRQYSYVRPVEQQFRRVSLNFQRPN 170

Query: 589 EETLSRHSILGPNGLRGQWQPPTSNIG 669
           EETLSRHSILGPNGL  +WQPP +N G
Sbjct: 171 EETLSRHSILGPNGLNAKWQPPRTNEG 197


>ref|XP_003525234.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1-like [Glycine max]
          Length = 509

 Score =  398 bits (1023), Expect(2) = e-155
 Identities = 200/301 (66%), Positives = 235/301 (78%), Gaps = 2/301 (0%)
 Frame = +3

Query: 723  RYPSNIDLTPIEIPSLQKPVEDNVFVQKDTS-SDLERLTLESVLSLNVDSHSLLTEELCS 899
            RYP+ IDL+P+EIPSLQ+P+ D    +KD S S+  +  LES+L+ + D  +   +E  S
Sbjct: 213  RYPTIIDLSPVEIPSLQQPLVDGSLTKKDNSISEQHKSDLESILTQSDDCKAKHADEAPS 272

Query: 900  VTTSSLDTGLMELDMQTSIIRQPSPSPVLAEVQDLMPVTSPQV-SNSVSEFATLPDELAR 1076
            + +        E+  Q  + RQPSP PVLAEVQDL+P   P V          + D + R
Sbjct: 273  LISFEAT----EIPAQIEVTRQPSPPPVLAEVQDLVPAMLPHVIEEGCKTEIPMSDSIVR 328

Query: 1077 SKCPLQLHISTTMMEHFLSLAKSNTDKNLETCGILAGSLKNRKFFITALIIPKQESTSDS 1256
            ++ PLQLHIST+MME F+ LAKSNTDKNLETCGILAG LKNRKF+IT LIIPKQE+TS S
Sbjct: 329  AESPLQLHISTSMMESFMKLAKSNTDKNLETCGILAGLLKNRKFYITTLIIPKQEATSSS 388

Query: 1257 CQATNEEEIFDVQDKQSLFQLGWIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVMAPK 1436
            CQATNEEEIF+VQDKQSLF LGWIHTHP+QSCFMSSID+HTHYSYQIMLPEAVAIVMAP 
Sbjct: 389  CQATNEEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPT 448

Query: 1437 DSSRTHGIFRLTSPGGVSVIRNCDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDVIDL 1616
            DSSR HGIFRLT+PGG+SVIR C +RGFHPH++PPDGGPIY  CTDVYMNP+LKFDVIDL
Sbjct: 449  DSSRNHGIFRLTTPGGMSVIRQCQQRGFHPHNQPPDGGPIYDTCTDVYMNPDLKFDVIDL 508

Query: 1617 R 1619
            R
Sbjct: 509  R 509



 Score =  177 bits (450), Expect(2) = e-155
 Identities = 100/219 (45%), Positives = 139/219 (63%), Gaps = 7/219 (3%)
 Frame = +1

Query: 34  SNGGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFRGEENVVDLFVMLMRFSSLIT 213
           S+   +IAA+  + DVDNRI+LR+YYRIADN+L+QA+IFR E N+VDL++ML+RFSSL++
Sbjct: 3   SSETINIAASTQKLDVDNRIALRFYYRIADNILKQADIFRAETNIVDLYIMLLRFSSLVS 62

Query: 214 ETIPCHKDYKLYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKVNRLATTQVDRWGH-H 390
           ETIP H+DY+   Q +K+                 P VQ++ ++ N     Q + W   H
Sbjct: 63  ETIPRHRDYRSSPQGKKEALRKKLLHSMNELEKLKPKVQQKINEFNSRRAYQPNGWEKCH 122

Query: 391 QSNYRDPSLEWPPVKKQLPKYNE----IRKTPRYV--GSEGTGSLVQQDKFSLSRPMEEQ 552
            +N+ D S   P  K+ L  YN+    I  T  +V  GS G        +FS  RP+EE 
Sbjct: 123 SNNFMDFS---PAKKQTLTSYNKKKAVIPTTGEFVYQGSRG-------QQFSSVRPVEEN 172

Query: 553 YRKVNLNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIG 669
            R+++L++ RPKEETLSRHSILGPNGL+GQW+PP S+ G
Sbjct: 173 MRRLSLSLLRPKEETLSRHSILGPNGLKGQWRPPASDKG 211


>ref|XP_003528952.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1-like [Glycine max]
          Length = 519

 Score =  388 bits (997), Expect(2) = e-154
 Identities = 195/304 (64%), Positives = 236/304 (77%), Gaps = 5/304 (1%)
 Frame = +3

Query: 723  RYPSNIDLTPIEIPSLQKPVEDNVFVQKDTS-SDLERLTLESVLSLNVDS--HSLLTEEL 893
            +YPSNIDL+P+E+PSLQ  +ED    +KD S ++  +  L+S+L+ + D   H     + 
Sbjct: 217  KYPSNIDLSPVELPSLQHSLEDESLKKKDNSIAEHHKSELDSILTQSEDCQPHPQPQPQH 276

Query: 894  CSVTTSSLDTGLMELDMQTSIIRQPSPSPVLAEVQDLMPVTSPQVSNSVSEFATLP--DE 1067
                 S +     E   +  +IRQPSP PVLAEVQDL+P  SP V+ +  +   +P  D 
Sbjct: 277  DQEPPSLISFETTETSARIEVIRQPSPPPVLAEVQDLVPAVSPCVNEAGCK-TEIPSSDS 335

Query: 1068 LARSKCPLQLHISTTMMEHFLSLAKSNTDKNLETCGILAGSLKNRKFFITALIIPKQEST 1247
                + P+QLHIST +ME F+ LAKSNT KNLETCG+LAG LKNRKF+ITALIIPKQEST
Sbjct: 336  SVHVEAPMQLHISTALMESFMKLAKSNTKKNLETCGVLAGLLKNRKFYITALIIPKQEST 395

Query: 1248 SDSCQATNEEEIFDVQDKQSLFQLGWIHTHPSQSCFMSSIDLHTHYSYQIMLPEAVAIVM 1427
            SDSCQ TNEEEIF+VQDK+SLF LGWIHTHP+QSCFMSSIDLHTHYSYQIMLPE+VAIVM
Sbjct: 396  SDSCQTTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSIDLHTHYSYQIMLPESVAIVM 455

Query: 1428 APKDSSRTHGIFRLTSPGGVSVIRNCDRRGFHPHDEPPDGGPIYQHCTDVYMNPNLKFDV 1607
            AP+DSSR HGIFRLT+PGG+SVI+ CD+RGFHPH +PPDGGPIY+ CTDVYMNP+LKF+V
Sbjct: 456  APRDSSRNHGIFRLTAPGGMSVIKQCDQRGFHPHSQPPDGGPIYKTCTDVYMNPDLKFEV 515

Query: 1608 IDLR 1619
            IDLR
Sbjct: 516  IDLR 519



 Score =  185 bits (469), Expect(2) = e-154
 Identities = 99/217 (45%), Positives = 135/217 (62%)
 Frame = +1

Query: 19  REMKTSNGGFSIAANVPRRDVDNRISLRYYYRIADNLLRQANIFRGEENVVDLFVMLMRF 198
           R   +     +IAA+  + DVDNRISLR+YYRIADN+LRQA+IFR E+N++DL+VML+RF
Sbjct: 2   RSSSSDRNRINIAASAQKVDVDNRISLRFYYRIADNILRQADIFRAEKNIIDLYVMLLRF 61

Query: 199 SSLITETIPCHKDYKLYSQREKDVFXXXXXXXXXXXXXXXPVVQERTDKVNRLATTQVDR 378
           SSL++ETIP H+DY+    R+K+                 PVVQ++ +++N     Q + 
Sbjct: 62  SSLVSETIPRHRDYRSSPPRQKESLKKKLLISLNELENLKPVVQQKINELNSKLAYQQNG 121

Query: 379 WGHHQSNYRDPSLEWPPVKKQLPKYNEIRKTPRYVGSEGTGSLVQQDKFSLSRPMEEQYR 558
            G   SN    SL++ PVKKQ      + K  R    E      +   FS  RP+EE  R
Sbjct: 122 QGKFSSN---NSLDFSPVKKQTSASYGLIKAVRPTAGEFVYQGSRSQPFSYVRPVEEHAR 178

Query: 559 KVNLNIPRPKEETLSRHSILGPNGLRGQWQPPTSNIG 669
           +++L +P PKEETLSRHSILGPNGL+GQW+PP  + G
Sbjct: 179 RLSLTLPPPKEETLSRHSILGPNGLKGQWRPPIIDKG 215