BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00006413
         (1356 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      342   e-129
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   330   e-123
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       326   e-120
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   293   e-110
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   273   2e-98

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  342 bits (877), Expect(2) = e-129
 Identities = 165/224 (73%), Positives = 192/224 (85%)
 Frame = -1

Query: 1311 PYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEAETLDSQLYEAPT 1132
            PYDDIKQLIR ELA+RLQWGYK T EEQ+AE+  L HSL+ MKIA E ETLDSQLYE P 
Sbjct: 26   PYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQMKIATEVETLDSQLYEIPI 85

Query: 1131 SFHKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTL 952
             F KI+ GS +K SCCYFK+DS TLD+AEIAMLDLYCERAQI+DG SVLDLGCGQGALTL
Sbjct: 86   EFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTL 145

Query: 951  FVAQKYKQCQVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTSDRILAIGL 772
             VAQKYK C+V A+TNS+SQKEYI+E+ R RNL+NVEV LADITT+E  +T DRIL I L
Sbjct: 146  HVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIEL 205

Query: 771  FEHMQNYKLLLKKISKWMAKDGLLFVDHICHKTLAYQYKALDED 640
            FEHM+NY+LLL+KIS+W++KDGLLF++HICHKT AY Y+ LD+D
Sbjct: 206  FEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDD 249



 Score =  147 bits (372), Expect(2) = e-129
 Identities = 65/88 (73%), Positives = 77/88 (87%)
 Frame = -3

Query: 532 LYFQDDVSVVNHWILNGNHFSQTHGEWLKRFDANIVTIKQIFKSLTGNGEEALKMINFWR 353
           LYFQDDVSVVNHW L+G HFS+T+ EWLKR DAN+  IK +F++L GN EEA+K+IN+WR
Sbjct: 271 LYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLINYWR 330

Query: 352 MFCLSGIELFGYNNGEEWMTSHVLFKKK 269
            FCLSG+E+FGYNNGEEWM SHVLFKKK
Sbjct: 331 GFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  330 bits (845), Expect(2) = e-123
 Identities = 157/224 (70%), Positives = 191/224 (85%)
 Frame = -1

Query: 1311 PYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEAETLDSQLYEAPT 1132
            P D+IK+LIRI+L +RLQWG KST EEQ+A+++ L HSL+ MKIA E ETLD Q+YE P 
Sbjct: 28   PDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVETLDDQMYEVPI 87

Query: 1131 SFHKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTL 952
             F KI+ GS +K SCCYFK+DS TLD+AEIAML+LYCERAQIKDG+SVLDLGCGQGALTL
Sbjct: 88   DFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIKDGHSVLDLGCGQGALTL 147

Query: 951  FVAQKYKQCQVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTSDRILAIGL 772
            +VAQKYK  +V A+TNS+SQKE+I+E+ R RNL NVEV+LADITT++  DT DRIL + L
Sbjct: 148  YVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADITTHKMPDTYDRILVVEL 207

Query: 771  FEHMQNYKLLLKKISKWMAKDGLLFVDHICHKTLAYQYKALDED 640
            FEHM+NY+LLL+KI +WMAKDGLLFV+HICHKT AY Y+ +DED
Sbjct: 208  FEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDED 251



 Score =  140 bits (354), Expect(2) = e-123
 Identities = 63/89 (70%), Positives = 76/89 (85%), Gaps = 1/89 (1%)
 Frame = -3

Query: 532 LYFQDDVSVVNHWILNGNHFSQTHGEWLKRFDANIVTIKQIFKSLTGN-GEEALKMINFW 356
           LYFQDDVSVVNHW L+G HFS+T+ EWLKR DAN+  IK +F ++TG   +EA+K+IN+W
Sbjct: 273 LYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVTITGQCRQEAMKLINYW 332

Query: 355 RMFCLSGIELFGYNNGEEWMTSHVLFKKK 269
           R FCLSG+E+FGYNNGEEWM SHVLFKKK
Sbjct: 333 RGFCLSGMEMFGYNNGEEWMASHVLFKKK 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  326 bits (836), Expect(2) = e-120
 Identities = 155/224 (69%), Positives = 188/224 (83%)
 Frame = -1

Query: 1311 PYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEAETLDSQLYEAPT 1132
            P D++K+L+RI+LA+RLQWGYKST EEQ+A+VL L HSL+HM IA E +TLDS +YE P 
Sbjct: 19   PNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVDTLDSNMYEVPL 78

Query: 1131 SFHKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTL 952
             F KI+ GS +K S CYFKDDS TLD+AEIAM+DL+CERAQIKDG+SVLDLGCG G+LT 
Sbjct: 79   DFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVLDLGCGHGSLTF 138

Query: 951  FVAQKYKQCQVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTSDRILAIGL 772
            +VAQKYK+  V A+TNS+SQKEYI+E+ R RNL NVEV+LADI T+E  DT DRIL +GL
Sbjct: 139  YVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMADTYDRILVVGL 198

Query: 771  FEHMQNYKLLLKKISKWMAKDGLLFVDHICHKTLAYQYKALDED 640
            FEHM+NY LLLKKIS+WMAKDGLLFV H+CHKT A+  + +DED
Sbjct: 199  FEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDED 242



 Score =  134 bits (338), Expect(2) = e-120
 Identities = 58/88 (65%), Positives = 71/88 (80%)
 Frame = -3

Query: 532 LYFQDDVSVVNHWILNGNHFSQTHGEWLKRFDANIVTIKQIFKSLTGNGEEALKMINFWR 353
           LYFQDD SVVNHW L+G HFS+T  EWLKR DAN+  I  +F ++TG+ EEA+K++N+WR
Sbjct: 264 LYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFATITGSEEEAVKLLNYWR 323

Query: 352 MFCLSGIELFGYNNGEEWMTSHVLFKKK 269
             C  G+EL+GYNNGEEWM SHVLFKKK
Sbjct: 324 GLCFFGVELYGYNNGEEWMESHVLFKKK 351


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  293 bits (751), Expect(2) = e-110
 Identities = 141/224 (62%), Positives = 178/224 (79%)
 Frame = -1

Query: 1311 PYDDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEAETLDSQLYEAPT 1132
            P  +I+QLIR+EL KRLQWGYK T EEQ++++L LVHSL+ MK+A E E LD +LYEAP 
Sbjct: 19   PDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPM 78

Query: 1131 SFHKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTL 952
             F KI  GS MK+S  Y+ D+S TLD+AEIAMLDLY ERAQIKDG SVLDLGCG GA+ L
Sbjct: 79   EFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVAL 138

Query: 951  FVAQKYKQCQVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTSDRILAIGL 772
            F A K+K+CQ   +T+S+ QK+YI+ +C+   L NV+V+LADITTYET++  DRI A+ L
Sbjct: 139  FGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVEL 198

Query: 771  FEHMQNYKLLLKKISKWMAKDGLLFVDHICHKTLAYQYKALDED 640
             EHM+NY+LLLKKIS+WM  DGLLFV+H+CHKTLAY Y+ +D +
Sbjct: 199  IEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAE 242



 Score =  133 bits (334), Expect(2) = e-110
 Identities = 56/88 (63%), Positives = 71/88 (80%)
 Frame = -3

Query: 532 LYFQDDVSVVNHWILNGNHFSQTHGEWLKRFDANIVTIKQIFKSLTGNGEEALKMINFWR 353
           LYFQDDVSVVN W L+G H+S++H EWLK  D NIV  K+I +S+T   +EA+K++NFWR
Sbjct: 264 LYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRSITKTEKEAIKLLNFWR 323

Query: 352 MFCLSGIELFGYNNGEEWMTSHVLFKKK 269
           +FC+ G ELFGY NGEEWM +H+LFKKK
Sbjct: 324 IFCMCGAELFGYKNGEEWMLTHLLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  273 bits (699), Expect(2) = 2e-98
 Identities = 126/222 (56%), Positives = 175/222 (78%)
 Frame = -1

Query: 1305 DDIKQLIRIELAKRLQWGYKSTCEEQVAEVLKLVHSLQHMKIANEAETLDSQLYEAPTSF 1126
            ++I+ +++I++ KRL+WGYK T E+Q+A+++    SL+ M++A E +TLD++LYE P  F
Sbjct: 26   EEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDTLDAELYEIPLPF 85

Query: 1125 HKIIFGSTMKESCCYFKDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTLFV 946
              I+ G T+K S  YFKD+S TLD++E+ M+DLYCERAQIKDG S+LDLGCG G+LTL V
Sbjct: 86   LHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKDGQSILDLGCGHGSLTLHV 145

Query: 945  AQKYKQCQVKAITNSISQKEYIDEQCRARNLVNVEVVLADITTYETDDTSDRILAIGLFE 766
            AQKY+ C+V  ITNS+SQKE+I +QC+  +L NVE++L D+T +ET+ T DRI A+ L E
Sbjct: 146  AQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIE 205

Query: 765  HMQNYKLLLKKISKWMAKDGLLFVDHICHKTLAYQYKALDED 640
            HM+NY+L LKK+S W+A+ GLLFV+H CHK  AYQY+ LDED
Sbjct: 206  HMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDED 247



 Score =  113 bits (283), Expect(2) = 2e-98
 Identities = 50/88 (56%), Positives = 66/88 (75%)
 Frame = -3

Query: 532 LYFQDDVSVVNHWILNGNHFSQTHGEWLKRFDANIVTIKQIFKSLTGNGEEALKMINFWR 353
           LYFQ+DVSVVNHW L+G H S    +WLKR D NI  +K+IF+S  G+ E+A+K I +WR
Sbjct: 269 LYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYGSKEKAMKFITYWR 328

Query: 352 MFCLSGIELFGYNNGEEWMTSHVLFKKK 269
           +FC++  +++  NNGEEWM S VLFKKK
Sbjct: 329 VFCIAHSQMYSTNNGEEWMLSQVLFKKK 356