BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00006365
         (3821 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264362.2| PREDICTED: TEL2-interacting protein 1 homolo...  1375   0.0  
emb|CBI24199.3| unnamed protein product [Vitis vinifera]             1369   0.0  
ref|XP_002310678.1| predicted protein [Populus trichocarpa] gi|2...  1280   0.0  
ref|XP_002517107.1| conserved hypothetical protein [Ricinus comm...  1266   0.0  
ref|XP_004139893.1| PREDICTED: uncharacterized protein LOC101212...  1199   0.0  

>ref|XP_002264362.2| PREDICTED: TEL2-interacting protein 1 homolog [Vitis vinifera]
          Length = 1390

 Score = 1375 bits (3559), Expect = 0.0
 Identities = 701/1153 (60%), Positives = 885/1153 (76%), Gaps = 31/1153 (2%)
 Frame = -1

Query: 3602 WTLVLTPSQDTEAARGHRGSARLRVEAFLTLRVLIAKVGTADALAFFLPGVASQFGKVLH 3423
            W  +L  + DTEA RGHRGSA+LRVEAFL+LR+L+AKVG+ADALAFFLPGV SQF KVL+
Sbjct: 236  WLSLLLKAADTEAQRGHRGSAKLRVEAFLSLRMLVAKVGSADALAFFLPGVVSQFSKVLY 295

Query: 3422 ISKTMISGAAGSTEAIEQATRGLVEFLMIVLQDKANLSGLDMLVNESTVFHLSEDKSTKS 3243
            +SKTMISGAAGS EAI+QA RG+ EFLM+VL+D ANLSGLD ++      H ++D+ST+S
Sbjct: 296  VSKTMISGAAGSVEAIDQAIRGVAEFLMVVLRDDANLSGLDNVIAGC---HTNKDESTQS 352

Query: 3242 VVEALRRLPVNRENQDETLATDSSDQ---AVVLKVDSKDK---CINNNVGFLCVNRTKDW 3081
             +E LR+LP+  + Q ET+A DSS +   ++  K   ++K        +G L V RTKDW
Sbjct: 353  FLEELRQLPLKAQGQSETIAEDSSGEIISSISPKFGFEEKGSISSRKMLGSLHVTRTKDW 412

Query: 3080 IKETSVHVDKLMSATFPHLCVHPAKKVRQGLVDSILGLLSECKHTLKRSRXXXXXXXXXX 2901
            I++TS  VDKL+  TFP +CVHPAKKVR+GL+ +I GLLS+C HTLK+SR          
Sbjct: 413  IEKTSTQVDKLLCTTFPKICVHPAKKVRRGLLVAIQGLLSKCSHTLKKSRLMLLECLCVL 472

Query: 2900 XCDDSEDVSVVAQDLLESFFKVGEKHLIEREIAEIFNRLIEKLPRVVLGSDATNAVAYAQ 2721
             CDDSE+VS VAQ  LE  F   +KH IE ++AEIF+RLIE LP+VVLGS+ + A+++AQ
Sbjct: 473  VCDDSEEVSAVAQGFLEYLFSSSDKHHIECDVAEIFSRLIENLPKVVLGSEESVALSHAQ 532

Query: 2720 QLLSFMYYSGPQLVVDNLLRSPLTASRFLEVLTLCIDQNSVFAGSLDKFISERPLSVGYL 2541
            QLL  +Y+SGPQ VVD+LL+SP+ A+RFL+V  LC+ QNSVF+GS+DK + ERP S GYL
Sbjct: 533  QLLVLIYFSGPQFVVDHLLQSPIKAARFLDVFALCLSQNSVFSGSIDKLLLERPSSTGYL 592

Query: 2540 LSIAELRSG-----------SHTPTAY--------KHLQAPLDNVHQAYEVPKMPPWFRQ 2418
             S+AEL+S            S  P           K +Q PL+N+ + YE+P MPPWF  
Sbjct: 593  QSVAELKSSIRFTSDDQATLSTAPYEISKFAGLKDKEIQYPLENMQKDYELPHMPPWFVY 652

Query: 2417 SGSQKLYQVLAGILRLAGLSIMADSRREVSLSLIIEIPLNYLRQLISEVRVKQYRKESWN 2238
             GSQKLY+ LAGILRL GLS MAD R E  LS+I +IPL Y R+L+SEVR+++Y KESW 
Sbjct: 653  VGSQKLYKALAGILRLVGLSTMADFRSEGYLSVITDIPLGYFRKLVSEVRMREYSKESWQ 712

Query: 2237 SFYRRSGLGQLVRQASTAACILNEVIYGLSDQSVEDFAKMFRRSRFKGDRPQGDEVVYSI 2058
            S+Y R+G GQL+RQASTAAC+LNE+I+G+SDQ+VEDFA+MF++S+   +  +G       
Sbjct: 713  SWYHRTGSGQLLRQASTAACMLNEMIFGISDQAVEDFARMFQKSKINQENMKG------Y 766

Query: 2057 ESVWKVCHGVDVRGHLIDCIGSILHEYLSPEVWDIPIDSKLATSQMDYDAEDITLHLFHD 1878
            +S+W+V  G   R HLIDCIG+I+HEYLS EVWD+P + K +  Q D +A + +LH   D
Sbjct: 767  DSIWRVWQGRGARSHLIDCIGNIMHEYLSSEVWDLPTEQKSSLLQADGEAGNFSLHFLCD 826

Query: 1877 TTMLHQ------VIIDGIGTFNVCLGKEFSSSGFLHSALYLLLENLICSSSEIRRASDAV 1716
            TT+LHQ      VIIDGIG FN+CLG +F+SSGFLHS+LYLLLENLIC + +IRRA DA+
Sbjct: 827  TTLLHQEIYSFFVIIDGIGIFNICLGNDFASSGFLHSSLYLLLENLICPNFQIRRACDAI 886

Query: 1715 LHVISASSGYPTVGCLVVANADYIIDSLCRELRHLDLNPHVANVVAAMLSYVGVANEILP 1536
            LHV++ +SGY TVG LV+ NADY+IDS+CR+LRHLDLNPHV NV+ AMLSY+G+A++ILP
Sbjct: 887  LHVLATTSGYSTVGHLVLENADYVIDSICRQLRHLDLNPHVPNVLGAMLSYIGIAHKILP 946

Query: 1535 LLEEPMRCVSLELEVLGRHQHPNLTMPFLKGVAEIVKASKCEACVMPTQAVEYSRRVRSK 1356
            LLEEPMR VS+ELE+LGRHQHP+LT+PFLK VAEI KASK EAC MP Q   YS  V+SK
Sbjct: 947  LLEEPMRTVSMELEILGRHQHPDLTIPFLKAVAEIAKASKKEACSMPIQTESYSIHVKSK 1006

Query: 1355 VSDVEKTKGKEFENGFAFHYGSDTNVNSIETEDSCSSGRGSDMELEQLEEMLFKLNESRR 1176
            +SDVEK    +        Y  D + +  E+E +      +DM L++ E +LFKLN+S+R
Sbjct: 1007 MSDVEKKARVDSGKSSISCYEEDMDTSPEESEGADIYLNDADMHLDEWESILFKLNDSKR 1066

Query: 1175 YRRIVGSIAGSCLTAASPLLASANESACLVALGIVEDGITALAKVEEAFRHEKGTEEEIK 996
            YRR VGSIA SCLTAA+PL+AS N++ACLVAL IVEDGI  LAKVEEA+RHEK T+E I+
Sbjct: 1067 YRRTVGSIASSCLTAATPLVASVNQAACLVALDIVEDGIATLAKVEEAYRHEKETKEAIE 1126

Query: 995  HAIQLCSFHDLQDTLDAADEGADENRLLPAMNKLWPYLVICIKNKNPLAIRRCLSMVSTT 816
              I++CSF+ LQDTLDAA+EG DENRLLPAMNK+WP+LV+CI+NKNP+A+RRCL ++S  
Sbjct: 1127 RVIKMCSFYHLQDTLDAAEEGTDENRLLPAMNKIWPFLVVCIRNKNPVAVRRCLDVMSKV 1186

Query: 815  VQICGGDFFSRRFHNDGPHFWKLLTTSPFTNKPILRDEKTPLQLPYRIVPKSSEDSMAET 636
            + ICGGDFFSRRFH DG HFWKLLTTSPF  +P+ ++E+ PLQLPYR  P S EDSMAE 
Sbjct: 1187 IHICGGDFFSRRFHTDGTHFWKLLTTSPFQKQPVSKEERIPLQLPYRSAPTSPEDSMAEV 1246

Query: 635  SSLKVQAAALNMIADLSRNKKSKSALQAVLKKVSGLVVGIACSSIAGLHDASINALSGLA 456
            S+LKVQAA LNMIADLS NK+S SAL+AVLKKVSGLVVGIACSS++GL DA++NAL+GL+
Sbjct: 1247 STLKVQAAMLNMIADLSLNKRSASALEAVLKKVSGLVVGIACSSVSGLRDAALNALTGLS 1306

Query: 455  HIDPDLIWLLLADVYYSLKKKDVPSPPSTGLQEISQLLPSPSSHKEFLFAQYGGESFGFG 276
             IDPDLIWLLLADVYY+ +KK +PSPP++ L EISQ+LP PSS K++L+ QYGG+S+GF 
Sbjct: 1307 SIDPDLIWLLLADVYYTFRKKHIPSPPTSDLPEISQILPPPSSPKDYLYVQYGGQSYGFD 1366

Query: 275  VDVPSVERVFQKM 237
            VD  SVE VFQK+
Sbjct: 1367 VDFSSVEIVFQKL 1379



 Score =  105 bits (261), Expect = 1e-19
 Identities = 51/82 (62%), Positives = 60/82 (73%)
 Frame = -2

Query: 3820 EFREGIVRCFKALLLRLHPCSDKSCVCGQIRGLPALISSTNSQIPHTAYLKPRSESVECL 3641
            EFREG++RCF+AL+L L PCSD SC C Q  G P L++S + Q+P     K  SE  ECL
Sbjct: 161  EFREGVIRCFRALILSLQPCSDMSCSCKQSLGFPILLASGDLQVPLVNTSKYDSEPGECL 220

Query: 3640 LAFLQSQNASAAVGHWFSLLLK 3575
            +AFLQSQ ASAAVGHW SLLLK
Sbjct: 221  IAFLQSQGASAAVGHWLSLLLK 242


>emb|CBI24199.3| unnamed protein product [Vitis vinifera]
          Length = 1386

 Score = 1369 bits (3544), Expect = 0.0
 Identities = 701/1153 (60%), Positives = 881/1153 (76%), Gaps = 31/1153 (2%)
 Frame = -1

Query: 3602 WTLVLTPSQDTEAARGHRGSARLRVEAFLTLRVLIAKVGTADALAFFLPGVASQFGKVLH 3423
            W  +L  + DTEA RGHRGSA+LRVEAFL+LR+L+AKVG+ADALAFFLPGV SQF KVL+
Sbjct: 236  WLSLLLKAADTEAQRGHRGSAKLRVEAFLSLRMLVAKVGSADALAFFLPGVVSQFSKVLY 295

Query: 3422 ISKTMISGAAGSTEAIEQATRGLVEFLMIVLQDKANLSGLDMLVNESTVFHLSEDKSTKS 3243
            +SKTMISGAAGS EAI+QA RG+ EFLM+VL+D ANLSGLD ++      H ++D+ST+S
Sbjct: 296  VSKTMISGAAGSVEAIDQAIRGVAEFLMVVLRDDANLSGLDNVIAGC---HTNKDESTQS 352

Query: 3242 VVEALRRLPVNRENQDETLATDSSDQ---AVVLKVDSKDK---CINNNVGFLCVNRTKDW 3081
             +E LR+LP+  + Q ET+A DSS +   ++  K   ++K        +G L V RTKDW
Sbjct: 353  FLEELRQLPLKAQGQSETIAEDSSGEIISSISPKFGFEEKGSISSRKMLGSLHVTRTKDW 412

Query: 3080 IKETSVHVDKLMSATFPHLCVHPAKKVRQGLVDSILGLLSECKHTLKRSRXXXXXXXXXX 2901
            I++TS  VDKL+  TFP +CVHPAKKVR+GL+ +I GLLS+C HTLK+SR          
Sbjct: 413  IEKTSTQVDKLLCTTFPKICVHPAKKVRRGLLVAIQGLLSKCSHTLKKSRLMLLECLCVL 472

Query: 2900 XCDDSEDVSVVAQDLLESFFKVGEKHLIEREIAEIFNRLIEKLPRVVLGSDATNAVAYAQ 2721
             CDDSE+VS VAQ  LE  F   +KH IE ++AEIF+RLIE LP+VVLGS+ + A+++AQ
Sbjct: 473  VCDDSEEVSAVAQGFLEYLFSSSDKHHIECDVAEIFSRLIENLPKVVLGSEESVALSHAQ 532

Query: 2720 QLLSFMYYSGPQLVVDNLLRSPLTASRFLEVLTLCIDQNSVFAGSLDKFISERPLSVGYL 2541
            QLL  +Y+SGPQ VVD+LL+SP+ A+RFL+V  LC+ QNSVF+GS+DK + ERP S GYL
Sbjct: 533  QLLVLIYFSGPQFVVDHLLQSPIKAARFLDVFALCLSQNSVFSGSIDKLLLERPSSTGYL 592

Query: 2540 LSIAELRSG-----------SHTPTAY--------KHLQAPLDNVHQAYEVPKMPPWFRQ 2418
             S+AEL+S            S  P           K +Q PL+N+ + YE+P MPPWF  
Sbjct: 593  QSVAELKSSIRFTSDDQATLSTAPYEISKFAGLKDKEIQYPLENMQKDYELPHMPPWFVY 652

Query: 2417 SGSQKLYQVLAGILRLAGLSIMADSRREVSLSLIIEIPLNYLRQLISEVRVKQYRKESWN 2238
             GSQKLY+ LAGILRL GLS MAD R E  LS+I +IPL Y R+L+SEVR+++Y KESW 
Sbjct: 653  VGSQKLYKALAGILRLVGLSTMADFRSEGYLSVITDIPLGYFRKLVSEVRMREYSKESWQ 712

Query: 2237 SFYRRSGLGQLVRQASTAACILNEVIYGLSDQSVEDFAKMFRRSRFKGDRPQGDEVVYSI 2058
            S+Y R+G GQL+RQASTAAC+LNE+I+G+SDQ+VEDFA+MF++           E     
Sbjct: 713  SWYHRTGSGQLLRQASTAACMLNEMIFGISDQAVEDFARMFQKH----------EAPMIN 762

Query: 2057 ESVWKVCHGVDVRGHLIDCIGSILHEYLSPEVWDIPIDSKLATSQMDYDAEDITLHLFHD 1878
            ES+W+V  G   R HLIDCIG+I+HEYLS EVWD+P + K +  Q D +A + +LH   D
Sbjct: 763  ESIWRVWQGRGARSHLIDCIGNIMHEYLSSEVWDLPTEQKSSLLQADGEAGNFSLHFLCD 822

Query: 1877 TTMLHQ------VIIDGIGTFNVCLGKEFSSSGFLHSALYLLLENLICSSSEIRRASDAV 1716
            TT+LHQ      VIIDGIG FN+CLG +F+SSGFLHS+LYLLLENLIC + +IRRA DA+
Sbjct: 823  TTLLHQEIYSFFVIIDGIGIFNICLGNDFASSGFLHSSLYLLLENLICPNFQIRRACDAI 882

Query: 1715 LHVISASSGYPTVGCLVVANADYIIDSLCRELRHLDLNPHVANVVAAMLSYVGVANEILP 1536
            LHV++ +SGY TVG LV+ NADY+IDS+CR+LRHLDLNPHV NV+ AMLSY+G+A++ILP
Sbjct: 883  LHVLATTSGYSTVGHLVLENADYVIDSICRQLRHLDLNPHVPNVLGAMLSYIGIAHKILP 942

Query: 1535 LLEEPMRCVSLELEVLGRHQHPNLTMPFLKGVAEIVKASKCEACVMPTQAVEYSRRVRSK 1356
            LLEEPMR VS+ELE+LGRHQHP+LT+PFLK VAEI KASK EAC MP Q   YS  V+SK
Sbjct: 943  LLEEPMRTVSMELEILGRHQHPDLTIPFLKAVAEIAKASKKEACSMPIQTESYSIHVKSK 1002

Query: 1355 VSDVEKTKGKEFENGFAFHYGSDTNVNSIETEDSCSSGRGSDMELEQLEEMLFKLNESRR 1176
            +SDVEK    +        Y  D + +  E+E +      +DM L++ E +LFKLN+S+R
Sbjct: 1003 MSDVEKKARVDSGKSSISCYEEDMDTSPEESEGADIYLNDADMHLDEWESILFKLNDSKR 1062

Query: 1175 YRRIVGSIAGSCLTAASPLLASANESACLVALGIVEDGITALAKVEEAFRHEKGTEEEIK 996
            YRR VGSIA SCLTAA+PL+AS N++ACLVAL IVEDGI  LAKVEEA+RHEK T+E I+
Sbjct: 1063 YRRTVGSIASSCLTAATPLVASVNQAACLVALDIVEDGIATLAKVEEAYRHEKETKEAIE 1122

Query: 995  HAIQLCSFHDLQDTLDAADEGADENRLLPAMNKLWPYLVICIKNKNPLAIRRCLSMVSTT 816
              I++CSF+ LQDTLDAA+EG DENRLLPAMNK+WP+LV+CI+NKNP+A+RRCL ++S  
Sbjct: 1123 RVIKMCSFYHLQDTLDAAEEGTDENRLLPAMNKIWPFLVVCIRNKNPVAVRRCLDVMSKV 1182

Query: 815  VQICGGDFFSRRFHNDGPHFWKLLTTSPFTNKPILRDEKTPLQLPYRIVPKSSEDSMAET 636
            + ICGGDFFSRRFH DG HFWKLLTTSPF  +P+ ++E+ PLQLPYR  P S EDSMAE 
Sbjct: 1183 IHICGGDFFSRRFHTDGTHFWKLLTTSPFQKQPVSKEERIPLQLPYRSAPTSPEDSMAEV 1242

Query: 635  SSLKVQAAALNMIADLSRNKKSKSALQAVLKKVSGLVVGIACSSIAGLHDASINALSGLA 456
            S+LKVQAA LNMIADLS NK+S SAL+AVLKKVSGLVVGIACSS++GL DA++NAL+GL+
Sbjct: 1243 STLKVQAAMLNMIADLSLNKRSASALEAVLKKVSGLVVGIACSSVSGLRDAALNALTGLS 1302

Query: 455  HIDPDLIWLLLADVYYSLKKKDVPSPPSTGLQEISQLLPSPSSHKEFLFAQYGGESFGFG 276
             IDPDLIWLLLADVYY+ +KK +PSPP++ L EISQ+LP PSS K++L+ QYGG+S+GF 
Sbjct: 1303 SIDPDLIWLLLADVYYTFRKKHIPSPPTSDLPEISQILPPPSSPKDYLYVQYGGQSYGFD 1362

Query: 275  VDVPSVERVFQKM 237
            VD  SVE VFQK+
Sbjct: 1363 VDFSSVEIVFQKL 1375



 Score =  105 bits (261), Expect = 1e-19
 Identities = 51/82 (62%), Positives = 60/82 (73%)
 Frame = -2

Query: 3820 EFREGIVRCFKALLLRLHPCSDKSCVCGQIRGLPALISSTNSQIPHTAYLKPRSESVECL 3641
            EFREG++RCF+AL+L L PCSD SC C Q  G P L++S + Q+P     K  SE  ECL
Sbjct: 161  EFREGVIRCFRALILSLQPCSDMSCSCKQSLGFPILLASGDLQVPLVNTSKYDSEPGECL 220

Query: 3640 LAFLQSQNASAAVGHWFSLLLK 3575
            +AFLQSQ ASAAVGHW SLLLK
Sbjct: 221  IAFLQSQGASAAVGHWLSLLLK 242


>ref|XP_002310678.1| predicted protein [Populus trichocarpa] gi|222853581|gb|EEE91128.1|
            predicted protein [Populus trichocarpa]
          Length = 1399

 Score = 1280 bits (3312), Expect = 0.0
 Identities = 673/1176 (57%), Positives = 853/1176 (72%), Gaps = 54/1176 (4%)
 Frame = -1

Query: 3602 WTLVLTPSQDTEAARGHRGSARLRVEAFLTLRVLIAKVGTADALAFFLPGVASQFGKVLH 3423
            W  +L  + D E ARGHRGSA++RVEAFLT+R L+AK+GTADALAFFLPGV SQF KVLH
Sbjct: 231  WFSLLLKAADNEVARGHRGSAKIRVEAFLTVRGLVAKIGTADALAFFLPGVVSQFAKVLH 290

Query: 3422 ISKTMISGAAGSTEAIEQATRGLVEFLMIVLQDKANLSGLDMLVNESTVFHLSEDKST-K 3246
            +SKTMISGAAGS EAI+QA R L E+LMIVL+D AN+S LD  +   + F+ ++  S+  
Sbjct: 291  MSKTMISGAAGSVEAIDQAIRALAEYLMIVLEDDANVSSLDRSLCAGSGFNSNKKGSSIH 350

Query: 3245 SVVEALRRLPVNRENQDETLATDSSDQAVVLKVDSKD---KCINNNVGFLCVNRTKDWIK 3075
            SV++ LR+LPV+ +NQ +  A +S  +AV     + +       N  G L V+RT+DW++
Sbjct: 351  SVLDELRQLPVSTQNQSKVAAENSVAEAVKSVTPASEFQSAKPGNEKGALHVDRTRDWVE 410

Query: 3074 ETSVHVDKLMSATFPHLCVHPAKKVRQGLVDSILGLLSECKHTLKRSRXXXXXXXXXXXC 2895
            ETS HVD+L+SATFPH+C+HPA+KVRQGL+  I GLLS+C  TLK+S+            
Sbjct: 411  ETSAHVDRLLSATFPHICLHPARKVRQGLLAVIRGLLSKCSCTLKQSKSMFLECLFVLVV 470

Query: 2894 DDSEDVSVVAQDLLESFFKVGEKHLIEREIAEIFNRLIEKLPRVVLGSDATNAVAYAQQL 2715
            D+  D+S  AQ+ LE       K  ++ ++AE+F+RL+EKLP+VV G+D ++A+++AQQL
Sbjct: 471  DECGDISAPAQEFLEYLLSSNSKLNVQSDVAELFSRLVEKLPKVVFGNDESHALSHAQQL 530

Query: 2714 LSFMYYSGPQLVVDNLLRSPLTASRFLEVLTLCIDQNSVFAGSLDKFISERPLSVGYLLS 2535
            L  +YYSGP+ ++D+L +SP+TA+RFL++  L + QNSVF G+LDK +  RP S+GYL S
Sbjct: 531  LVVIYYSGPKFLMDHL-QSPVTAARFLDIFALSLSQNSVFTGALDKLMLARPSSIGYLHS 589

Query: 2534 IAELRSGSHTPTAY-------------------KHLQAPLDNVHQAYEVPKMPPWFRQSG 2412
            IAEL+S S   + Y                   K +Q P  ++    E+P+MPPWF   G
Sbjct: 590  IAELKSSSRFSSDYQSIVDVVPSDNPNSRDIHGKAIQNPSLSLQDNSELPRMPPWF---G 646

Query: 2411 SQKLYQVLAGILRLAGLSIMADSRREVSLSLIIEIPLNYLRQLISEVRVKQYRKESWNSF 2232
            SQKLYQ LAGILRL GLS+M DS+ E  +S++ +IPL +LR+L+SE+R K++ KESW S+
Sbjct: 647  SQKLYQTLAGILRLVGLSLMTDSKSEGHMSVVSDIPLGHLRKLVSEIRDKEFTKESWQSW 706

Query: 2231 YRRSGLGQLVRQASTAACILNEVIYGLSDQSVEDFAKMFRRSRF----------KGDRPQ 2082
            Y R+G GQL+RQASTA CILNE+I+GLSDQ+V++  ++F  S            KG   Q
Sbjct: 707  YNRTGSGQLLRQASTAVCILNEMIFGLSDQAVDNLIRLFHTSELNREGVQAPDAKGADAQ 766

Query: 2081 GDEVVYS--IESVWKVCHGVDVRGHLIDCIGSILHEYLSPEVWDIPIDSKLATSQMDYDA 1908
             + V +     S+WKV      R HL DC+G I HEYLS EVW++PID K +  Q D + 
Sbjct: 767  PNTVEHPERTRSIWKVSQERVARSHLNDCVGRIAHEYLSSEVWNLPIDQKSSLVQSDGEV 826

Query: 1907 EDITLHLFHDTTMLHQVIIDGIGTFNVCLGKEFSSSGFLHSALYLLLENLICSSSEIRRA 1728
            E+ITLH FHDT ML QVIIDGIG F++CLGK+F+SS FLHS+LYLLLE+LICS+ ++R+A
Sbjct: 827  EEITLHFFHDTAMLQQVIIDGIGIFSMCLGKDFASSWFLHSSLYLLLESLICSNIQVRQA 886

Query: 1727 SDAVLHVISASSGYPTVGCLVVANADYIIDSLCRELRHLDLNPHVANVVAAMLSYVGVAN 1548
            SDAVLHV+S +SG+PTVG LV+ANADYIIDS+CR+LRHLDLNP V NV+A++LSY+GVA+
Sbjct: 887  SDAVLHVLSCASGHPTVGQLVLANADYIIDSICRQLRHLDLNPRVPNVLASLLSYIGVAH 946

Query: 1547 EILPLLEEPMRCVSLELEVLGRHQHPNLTMPFLKGVAEIVKASKCEACVMPTQAVEYSRR 1368
            +ILPLLEEPMR VS ELE+LGRHQHP LT+PFLK VAEI KASK EA  +PT A  Y   
Sbjct: 947  KILPLLEEPMRSVSQELEILGRHQHPVLTIPFLKAVAEIGKASKHEASSLPTNAESYLMH 1006

Query: 1367 VRSKVSDVEKTKGKEFENGFAFHYGSDTNVNSIETEDSCSSGRGSDMELEQLEEMLFKLN 1188
            V+SKVSD+ K K  E       +Y +D ++              SDME EQ E +LFKLN
Sbjct: 1007 VKSKVSDMGKGKKLESHENSTSYYDNDIDM--------------SDMESEQWENLLFKLN 1052

Query: 1187 ESRRYRRIVGSIAGSCLTAASPLLASANESACLVALGIVEDGITALAKVEEAFRHEKGTE 1008
            +S+RYRR VGSIAGSCLTAA PLLAS  +  CLVAL IVEDGI  L KVEEA+RHEK T+
Sbjct: 1053 DSKRYRRTVGSIAGSCLTAAIPLLASMKQEECLVALNIVEDGIVTLGKVEEAYRHEKETK 1112

Query: 1007 EEIKHAIQLCSFHDLQDTLDAADEGADENRLLPAMNKLWPYLVICIKNKNP--------- 855
            E I+  I+  S + LQDTLDAA+EG DENRLLPAMNK+WP+LV C++NKNP         
Sbjct: 1113 EAIEEVIRSYSLYQLQDTLDAAEEGTDENRLLPAMNKIWPFLVACVRNKNPVVRIHLFLL 1172

Query: 854  ----------LAIRRCLSMVSTTVQICGGDFFSRRFHNDGPHFWKLLTTSPFTNKPILRD 705
                      +A+RRCLS++S+ V ICGGDFFSRRFH DGPHFWKLLTTSP   KP  ++
Sbjct: 1173 EAALCGIPLVMAVRRCLSVISSVVLICGGDFFSRRFHTDGPHFWKLLTTSPLQKKPFSKE 1232

Query: 704  EKTPLQLPYRIVPKSSEDSMAETSSLKVQAAALNMIADLSRNKKSKSALQAVLKKVSGLV 525
            + TPLQLPYR  P SS DSM+E S+LKVQ A LNMIA LS+NK+S SALQ VLKKVSGLV
Sbjct: 1233 DTTPLQLPYRSAPTSSGDSMSEISNLKVQVAVLNMIAHLSQNKRSTSALQIVLKKVSGLV 1292

Query: 524  VGIACSSIAGLHDASINALSGLAHIDPDLIWLLLADVYYSLKKKDVPSPPSTGLQEISQL 345
            VGIA S + GLHDASINAL GLA ID DLIWLLLADVYY+LKKKD+PSPP +GL +IS++
Sbjct: 1293 VGIAFSGVKGLHDASINALRGLASIDSDLIWLLLADVYYALKKKDLPSPPISGLPQISKI 1352

Query: 344  LPSPSSHKEFLFAQYGGESFGFGVDVPSVERVFQKM 237
            LP P S K +L+ QYGG+SFGF +D PSVE VF+K+
Sbjct: 1353 LPPPLSPKGYLYVQYGGQSFGFDIDYPSVETVFKKL 1388



 Score = 85.5 bits (210), Expect = 1e-13
 Identities = 43/84 (51%), Positives = 61/84 (72%), Gaps = 2/84 (2%)
 Frame = -2

Query: 3820 EFREGIVRCFKALLLRLHPCSDKSCVCGQIRGLPALISSTNSQIPHTA--YLKPRSESVE 3647
            EFREG+++CF+AL+  L  C  + C C +I GLPAL+ + +++  ++A  YL       E
Sbjct: 157  EFREGVIKCFRALIEGLSSCGVEGCSCEEINGLPALVEAGDNRNVNSARDYLGGEG---E 213

Query: 3646 CLLAFLQSQNASAAVGHWFSLLLK 3575
            CL++FL+SQ+ASAAVGHWFSLLLK
Sbjct: 214  CLVSFLRSQSASAAVGHWFSLLLK 237


>ref|XP_002517107.1| conserved hypothetical protein [Ricinus communis]
            gi|223543742|gb|EEF45270.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 1377

 Score = 1266 bits (3275), Expect = 0.0
 Identities = 666/1153 (57%), Positives = 839/1153 (72%), Gaps = 31/1153 (2%)
 Frame = -1

Query: 3602 WTLVLTPSQDTEAARGHRGSARLRVEAFLTLRVLIAKVGTADALAFFLPGVASQFGKVLH 3423
            W  +L      EA RGHRG+A++RVEAFLTLRVL++KVGTADALAFFLPGV SQF +VLH
Sbjct: 233  WLSLLLKVHYIEATRGHRGNAKIRVEAFLTLRVLVSKVGTADALAFFLPGVISQFARVLH 292

Query: 3422 ISKTMISGAAGSTEAIEQATRGLVEFLMIVLQDKANLSGLDMLVNESTVFHLSEDKSTKS 3243
            +SKTMISGAAGS EA + A RGL E+LMIVL D AN S LD+  N+   F ++ ++S  S
Sbjct: 293  VSKTMISGAAGSVEATDHAIRGLAEYLMIVLCDDANFSSLDVSSNDLAGFSVNNNESIHS 352

Query: 3242 VVEALRRLPVNRENQDETLATDSSDQAVVLKVDSKDKCINNNVGFLCVNRTKDWIKETSV 3063
            +++ LR LP + + + + +A +S+ +A+ +   +++K     +G L V+RT+DWIK+T+V
Sbjct: 353  LLDELRHLPNSNQGKRDKVAEESNGEALNIGSPARNK-FGKEIGSLHVDRTRDWIKKTAV 411

Query: 3062 HVDKLMSATFPHLCVHPAKKVRQGLVDSILGLLSECKHTLKRSRXXXXXXXXXXXCDDSE 2883
            H++K++SATFPH+CVHPAKKVR+GL+ +I GLLS+C +TLK SR            DD +
Sbjct: 412  HLNKVLSATFPHICVHPAKKVREGLLGAIQGLLSKCSYTLKDSRLMLLECLCVLIVDDCK 471

Query: 2882 DVSVVAQDLLESFFKVGEKHLIEREIAEIFNRLIEKLPRVVLGSDATNAVAYAQQLLSFM 2703
            DVS  AQ  LE  F    KH ++ ++ EIF  LIEKLP+VVL ++ +  +++AQQLL  +
Sbjct: 472  DVSTPAQQFLEYLFSSSGKHHVQHDMTEIFGSLIEKLPKVVLRNEESLTLSHAQQLLVVI 531

Query: 2702 YYSGPQLVVDNLLRSPLTASRFLEVLTLCIDQNSVFAGSLDKFISERPLSVGYLLSIAEL 2523
            YYSGPQ V+D LL SP+TA+RFL+V  LC+ QNS F G+LDK    R  S GYL SIAEL
Sbjct: 532  YYSGPQFVLDQLL-SPVTAARFLDVFALCLSQNSAFTGALDKLTLARSHSAGYLPSIAEL 590

Query: 2522 RSGSHTPTAYKHLQ--APLD-----------------NVHQAYEVPKMPPWFRQSGSQKL 2400
            ++GSH    Y+ +   AP D                  V   YE+P+MPPWF   GSQKL
Sbjct: 591  KAGSHFANNYQVIMDAAPSDISKFSDVQGKRTQYSSKTVESNYELPRMPPWFAYVGSQKL 650

Query: 2399 YQVLAGILRLAGLSIMADSRREVSLSLIIEIPLNYLRQLISEVRVKQYRKESWNSFYRRS 2220
            Y+ LAGILRL GLS+M+D   E  +S++ +IPL+YLR+LISEVR K Y KE+W S+Y R+
Sbjct: 651  YRALAGILRLVGLSLMSDFGSEGHMSVVTDIPLDYLRKLISEVRAKDYTKETWQSWYNRT 710

Query: 2219 GLGQLVRQASTAACILNEVIYGLSDQSVEDFAKMFRRSRFKGD------------RPQGD 2076
            G GQL+R ASTAACILNE+I+GLSDQS++   KMF +S  KG+            +P   
Sbjct: 711  GSGQLLRHASTAACILNEMIFGLSDQSIDSLTKMFHKSMVKGEEIQEFDARGAGNQPCTF 770

Query: 2075 EVVYSIESVWKVCHGVDVRGHLIDCIGSILHEYLSPEVWDIPIDSKLATSQMDYDAEDIT 1896
            E      S+WK+      R  LI+CIG ILHEYLS EVWD+P+D K +  Q D +  +IT
Sbjct: 771  ECPELTRSIWKLSLEKASRVSLIECIGRILHEYLSSEVWDLPMDHKPSHVQPDDEVGEIT 830

Query: 1895 LHLFHDTTMLHQVIIDGIGTFNVCLGKEFSSSGFLHSALYLLLENLICSSSEIRRASDAV 1716
            LH FHDT MLHQVIIDGIG F VCLGK+F+SSGFLHS+LYLLLE LI S+  +R ASDAV
Sbjct: 831  LHFFHDTAMLHQVIIDGIGIFAVCLGKDFASSGFLHSSLYLLLEGLISSNFHVRIASDAV 890

Query: 1715 LHVISASSGYPTVGCLVVANADYIIDSLCRELRHLDLNPHVANVVAAMLSYVGVANEILP 1536
            LHV+SA+SG  TVG LV+ NADYIIDS+CR+LRHLDLNPHV  V+A+MLSY+GVA++I+P
Sbjct: 891  LHVLSATSGCQTVGQLVLENADYIIDSICRQLRHLDLNPHVPGVLASMLSYIGVAHKIMP 950

Query: 1535 LLEEPMRCVSLELEVLGRHQHPNLTMPFLKGVAEIVKASKCEACVMPTQAVEYSRRVRSK 1356
            LLEEPMR  S ELE+LGRHQHP LT+PFLK VAEI KASK EA  +   A  Y   V++K
Sbjct: 951  LLEEPMRSASQELEILGRHQHPELTIPFLKAVAEIAKASKREASSLLANAELYLSHVKAK 1010

Query: 1355 VSDVEKTKGKEFENGFAFHYGSDTNVNSIETEDSCSSGRGSDMELEQLEEMLFKLNESRR 1176
            V   EK    E   G   H  SD + N ++            ME +Q E +LF+LN+SRR
Sbjct: 1011 V---EKEVRLESRQGSPSH--SDNHTNMLQ------------MECDQWENILFQLNDSRR 1053

Query: 1175 YRRIVGSIAGSCLTAASPLLASANESACLVALGIVEDGITALAKVEEAFRHEKGTEEEIK 996
            +RR VGSIA SCLTAA+PLLAS  ++ACL+AL IVEDG+T LAKVEEA R+E  T+E I+
Sbjct: 1054 FRRTVGSIAVSCLTAATPLLASVKQAACLIALDIVEDGMTTLAKVEEAHRYENQTKEMIE 1113

Query: 995  HAIQLCSFHDLQDTLDAADEGADENRLLPAMNKLWPYLVICIKNKNPLAIRRCLSMVSTT 816
              I+  SF+ L DTL+AA+EG +ENRLLPAMNK+WP+LV CI+NKNP+A+RRCLS VS  
Sbjct: 1114 DVIRSYSFYHLHDTLEAAEEGNNENRLLPAMNKIWPFLVACIRNKNPVAVRRCLSTVSNV 1173

Query: 815  VQICGGDFFSRRFHNDGPHFWKLLTTSPFTNKPILRDEKTPLQLPYRIVPKSSEDSMAET 636
            VQICGGDFFSRRFH DG HFWKLL+TSPF  +P  ++E+ PLQLPYR  P S EDSMAE 
Sbjct: 1174 VQICGGDFFSRRFHTDGTHFWKLLSTSPFQKRPFSKEERIPLQLPYRSTPTSPEDSMAEV 1233

Query: 635  SSLKVQAAALNMIADLSRNKKSKSALQAVLKKVSGLVVGIACSSIAGLHDASINALSGLA 456
            SSLKVQAA LNMIADLSRNK+S S+L+AVLKKVSG+VVGIACS +AGLH+A++NAL+GLA
Sbjct: 1234 SSLKVQAAVLNMIADLSRNKRSASSLEAVLKKVSGVVVGIACSGVAGLHEAAVNALNGLA 1293

Query: 455  HIDPDLIWLLLADVYYSLKKKDVPSPPSTGLQEISQLLPSPSSHKEFLFAQYGGESFGFG 276
             ID DLIWLLLADVYYSLKKK  PSPP++    +SQ+LP P S K +L+ Q GG+S+GF 
Sbjct: 1294 SIDSDLIWLLLADVYYSLKKKGQPSPPTSSFPPMSQILPPPLSPKGYLYVQSGGQSYGFD 1353

Query: 275  VDVPSVERVFQKM 237
            +D+ SVE VF+K+
Sbjct: 1354 IDLSSVEAVFKKL 1366



 Score = 99.8 bits (247), Expect = 5e-18
 Identities = 50/83 (60%), Positives = 61/83 (73%)
 Frame = -2

Query: 3820 EFREGIVRCFKALLLRLHPCSDKSCVCGQIRGLPALISSTNSQIPHTAYLKPRSESVECL 3641
            EF EG+++CFKALLLRL PCSD++C C Q  GLPAL+ S + QI  T+     +E  ECL
Sbjct: 160  EFCEGVIKCFKALLLRLCPCSDEACSCRQSLGLPALLKSADMQICETS--NSDAEQGECL 217

Query: 3640 LAFLQSQNASAAVGHWFSLLLKI 3572
            LAFLQSQ A+  VGHW SLLLK+
Sbjct: 218  LAFLQSQAAAPGVGHWLSLLLKV 240


>ref|XP_004139893.1| PREDICTED: uncharacterized protein LOC101212708 [Cucumis sativus]
          Length = 1380

 Score = 1199 bits (3101), Expect = 0.0
 Identities = 626/1145 (54%), Positives = 822/1145 (71%), Gaps = 22/1145 (1%)
 Frame = -1

Query: 3602 WTLVLTPSQDTEAARGHRGSARLRVEAFLTLRVLIAKVGTADALAFFLPGVASQFGKVLH 3423
            W  +L  + D EA RGH GS+++R+EAF+TLR+L+AKVGTADALAFFLPGV SQF KVL 
Sbjct: 255  WLSLLLKAADIEATRGHLGSSKIRIEAFMTLRILVAKVGTADALAFFLPGVVSQFSKVLR 314

Query: 3422 ISKTMISGAAGSTEAIEQATRGLVEFLMIVLQDKANLSGLDMLVNESTVFHLSEDKSTKS 3243
             SKT +SGAAG+TEA  QA RGL E+LMIVL+++AN S L M ++  +   + + K  + 
Sbjct: 315  ASKTSLSGAAGNTEATNQAIRGLAEYLMIVLENEANKSSLVMFMDFQSEIIMEKGKKAQY 374

Query: 3242 VVEALRRLPVNRENQDETLATDSSDQAVVLKVD---------SKDKCINNNVGFLCVNRT 3090
            ++E LR+LP ++      +  + S   V  K           S D    NN     V+RT
Sbjct: 375  ILEELRQLP-DKVRSGSIMVGECSSAVVAKKTTYESGSKETMSADYLKGNNS--FHVDRT 431

Query: 3089 KDWIKETSVHVDKLMSATFPHLCVHPAKKVRQGLVDSILGLLSECKHTLKRSRXXXXXXX 2910
            K+W+ +TS HVDKL+ ATFP++C+H  KKVR G++ +I GLLS C  TLK SR       
Sbjct: 432  KEWVAQTSTHVDKLLRATFPYICMHLVKKVRLGILAAIEGLLSRCSCTLKESRSMLLECL 491

Query: 2909 XXXXCDDSEDVSVVAQDLLESFFKVGEKHLIEREIAEIFNRLIEKLPRVVLGSDATNAVA 2730
                 D+SEDVS  AQ+ LE  F +   H ++ ++A+IF RL+EKLP VVLG+D   A++
Sbjct: 492  CTLAIDESEDVSFTAQEFLEYLFWITGNHQLQHDVAKIFVRLVEKLPNVVLGTDEKFALS 551

Query: 2729 YAQQLLSFMYYSGPQLVVDNLLRSPLTASRFLEVLTLCIDQNSVFAGSLDKFISERPLSV 2550
            +A+QLL   YYSGPQL++D+L+ SP+TA RFL+V  +C++QNSV+A S+ KF+S RP S+
Sbjct: 552  HARQLLVVAYYSGPQLIIDHLIHSPVTAVRFLDVFAVCLNQNSVYACSIGKFLSARPSSL 611

Query: 2549 GYLLSIAELRSGSH------------TPTAYKHLQAPLDNVHQAYEV-PKMPPWFRQSGS 2409
            GYL S+ EL+ G++            +P   +       ++ Q   V P+MPPWF   G+
Sbjct: 612  GYLHSLTELKVGTNLISDCLSIMNTASPAVSELTMVQEKDIQQRNHVLPRMPPWFNGIGN 671

Query: 2408 QKLYQVLAGILRLAGLSIMADSRREVSLSLIIEIPLNYLRQLISEVRVKQYRKESWNSFY 2229
            QKLY+ L G+LRL GLS+ +D++ E SLS+ I+IPL  L++L+SE+R K+Y +E+W  +Y
Sbjct: 672  QKLYEALGGVLRLVGLSLASDNKGEGSLSVTIDIPLGNLQKLVSELRKKEYSEENWEYWY 731

Query: 2228 RRSGLGQLVRQASTAACILNEVIYGLSDQSVEDFAKMFRRSRFKGDRPQGDEVVYSIESV 2049
            RR+G GQLVRQASTA CILNE+I+G+S+ SV+ F+ MF+R+R         E V + E+ 
Sbjct: 732  RRTGSGQLVRQASTAVCILNEMIFGVSEHSVDYFSSMFQRARMHRKVTNDYECVTTNEAC 791

Query: 2048 WKVCHGVDVRGHLIDCIGSILHEYLSPEVWDIPIDSKLATSQMDYDAEDITLHLFHDTTM 1869
            WK+     +R  LIDCIG ILHEYLSPE+WD+P   K +      + +DI+LH F DT M
Sbjct: 792  WKISPE-KIRAQLIDCIGRILHEYLSPEIWDLPTQHKYSPMHSAGE-DDISLHFFRDTAM 849

Query: 1868 LHQVIIDGIGTFNVCLGKEFSSSGFLHSALYLLLENLICSSSEIRRASDAVLHVISASSG 1689
            LHQVII+GIG F++CLGK FSS GFLHS+LYLLLENLI S++E+R  SDA+LHV+S+SSG
Sbjct: 850  LHQVIIEGIGIFSMCLGKYFSSCGFLHSSLYLLLENLISSNAEVRSTSDAILHVLSSSSG 909

Query: 1688 YPTVGCLVVANADYIIDSLCRELRHLDLNPHVANVVAAMLSYVGVANEILPLLEEPMRCV 1509
            YPTV  LV+ NADY+IDS+CR+LRHLDLNPHV NV+AA+LSY+G+A+EILPLLEEPM  V
Sbjct: 910  YPTVRNLVLENADYVIDSICRQLRHLDLNPHVPNVLAAILSYIGIAHEILPLLEEPMHKV 969

Query: 1508 SLELEVLGRHQHPNLTMPFLKGVAEIVKASKCEACVMPTQAVEYSRRVRSKVSDVEKTKG 1329
            S ELE+LGRHQHPNLT PFLK VAEI + SK E+  +P++A  Y+  V+S +S  EK  G
Sbjct: 970  SSELEILGRHQHPNLTGPFLKAVAEIARVSKHESNSLPSKAASYTSHVKSLISKGEKQAG 1029

Query: 1328 KEFENGFAFHYGSDTNVNSIETEDSCSSGRGSDMELEQLEEMLFKLNESRRYRRIVGSIA 1149
                +     +  D N++S+E+E                E +LFKLN+SRRYRR VGSIA
Sbjct: 1030 GVSRSC----HDDDINISSLESE---------------WENILFKLNDSRRYRRTVGSIA 1070

Query: 1148 GSCLTAASPLLASANESACLVALGIVEDGITALAKVEEAFRHEKGTEEEIKHAIQLCSFH 969
            GSC+  A PLLAS  ++ CLVAL IVE G+ ALAKVEEA++HEK  +E I+  +   SF+
Sbjct: 1071 GSCIVTAIPLLASQKQATCLVALDIVEYGVAALAKVEEAYKHEKDIKEAIEETLHSHSFY 1130

Query: 968  DLQDTLDAADEGADENRLLPAMNKLWPYLVICIKNKNPLAIRRCLSMVSTTVQICGGDFF 789
             L DTLD ++EG+DENRLLPAMNK+WP+LV CI+NKNP+A RRCL+++S++VQICGGDFF
Sbjct: 1131 RLLDTLDVSEEGSDENRLLPAMNKIWPFLVACIQNKNPVAARRCLNVISSSVQICGGDFF 1190

Query: 788  SRRFHNDGPHFWKLLTTSPFTNKPILRDEKTPLQLPYRIVPKSSEDSMAETSSLKVQAAA 609
            +RRFH DG HFWKLLT+SPF  K  +R+EK  LQLPYR    SSEDS+AE S+LKVQ A 
Sbjct: 1191 TRRFHTDGSHFWKLLTSSPFLRKQNVREEKAVLQLPYRNTYISSEDSVAEGSNLKVQVAL 1250

Query: 608  LNMIADLSRNKKSKSALQAVLKKVSGLVVGIACSSIAGLHDASINALSGLAHIDPDLIWL 429
            LNMIADLSRN++S SAL+ VLKK+SGLV G+A S + GL +AS+NAL GLA IDPDLIWL
Sbjct: 1251 LNMIADLSRNRRSASALEVVLKKISGLVAGVAFSGVVGLREASLNALGGLASIDPDLIWL 1310

Query: 428  LLADVYYSLKKKDVPSPPSTGLQEISQLLPSPSSHKEFLFAQYGGESFGFGVDVPSVERV 249
            L+ADVYYS+ KKDVP PPS+   E+S+LLP PSS K +L+  YGG+S+GF ++V SVE V
Sbjct: 1311 LVADVYYSI-KKDVPLPPSSEFPEVSRLLPPPSSPKGYLYVLYGGQSYGFDIEVSSVEIV 1369

Query: 248  FQKMQ 234
            F+K+Q
Sbjct: 1370 FKKLQ 1374



 Score = 93.2 bits (230), Expect = 5e-16
 Identities = 48/97 (49%), Positives = 62/97 (63%)
 Frame = -2

Query: 3820 EFREGIVRCFKALLLRLHPCSDKSCVCGQIRGLPALISSTNSQIPHTAYLKPRSESVECL 3641
            EFREGI++CFKA+ + L+PCS+ +C C QI G PAL  +   Q  H       S+  ECL
Sbjct: 181  EFREGIIKCFKAIFMNLYPCSNDACSCKQISGSPALAENREFQ-GHLDVFSEESKPNECL 239

Query: 3640 LAFLQSQNASAAVGHWFSLLLKILRLHEDIAGVQGYV 3530
            L FL+S+ ASAAVGHW SLLLK      DI   +G++
Sbjct: 240  LEFLRSETASAAVGHWLSLLLKA----ADIEATRGHL 272