BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00006082
         (1139 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           285   2e-74
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    282   1e-73
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   277   3e-72
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]       275   2e-71
ref|XP_002284806.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   270   6e-70

>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  285 bits (728), Expect = 2e-74
 Identities = 150/374 (40%), Positives = 224/374 (59%), Gaps = 11/374 (2%)
 Frame = +2

Query: 50   MDFAAMLL---------QSALFILSLMVVYTKFWRKSATSNPSSANNKRXXXXXXXXXXX 202
            MDF+++LL          S   +L+L++VY     KS++S  +S   K+           
Sbjct: 1    MDFSSLLLLLLNTWISAYSMAALLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAVTGA 60

Query: 203  X-ILGHLHMLMGDELPHHVLGKLADKHGPAFLIHIGPHPELVISSWELAKECFTTNDKYF 379
              + GHLH+    E PH +L KLA+K+GP+F +  G H  LV+S   + KECFTTND  F
Sbjct: 61   WPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTNDTLF 120

Query: 380  VNRPSNKAFKYLSYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFF 559
             NRPS  AF  ++Y   S+ F PY P W E+RK+S   LLSN R++  KQ+R +EV   F
Sbjct: 121  SNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVSVCF 180

Query: 560  NELYQLCSTPNNNDATGSNSSYDRDGAVVGRGPVIVEMNKWFEELTLNVVTRMVSGKQHV 739
             ELY L +  N+N A                 PV +++ +WF+E++ NV+ R++ GKQ+ 
Sbjct: 181  KELYDLTNKKNDNGA-----------------PVPIDLKRWFDEVSNNVIMRVIFGKQNF 223

Query: 740  GTNARHG-DSEAKYYKKVIDDAARFMGNHVISDIFPSLGWLDYLLGHDGAMKKTGKELDS 916
            G+    G D EA +YKK++D+ +R     ++SD+ P LGWLDY  G   AMK+ GKEL+S
Sbjct: 224  GSKIVLGEDQEAVHYKKIMDELSRLSSLTMLSDMVPLLGWLDYFKGDLRAMKRNGKELNS 283

Query: 917  IFDSWVKEHQQKRKVQGSSNKNHEEDFIDITLSVMEESQLHGLDIETFIKSICVGMIFGG 1096
            I   W++EH+ K+      + +  +DF+D+ LS+ +++QL+G D +TFIK+ C+ MI GG
Sbjct: 284  ILQKWLEEHKSKK------SSDARQDFMDVMLSISKDTQLYGHDQDTFIKATCLAMIMGG 337

Query: 1097 SDTTSVALTWVLSL 1138
            +++T VALTW+LSL
Sbjct: 338  TNSTEVALTWILSL 351


>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  282 bits (721), Expect = 1e-73
 Identities = 142/354 (40%), Positives = 212/354 (59%)
 Frame = +2

Query: 77   SALFILSLMVVYTKFWRKSATSNPSSANNKRXXXXXXXXXXXXILGHLHMLMGDELPHHV 256
            S   I++ + +Y  F       +  +  NK+            +LGHLH+    ELPH +
Sbjct: 13   SVASIIAFVFLYNLF-------SSRTLKNKKIRTAPMATGAWPVLGHLHLFGSGELPHKM 65

Query: 257  LGKLADKHGPAFLIHIGPHPELVISSWELAKECFTTNDKYFVNRPSNKAFKYLSYDQASV 436
            L  +ADK+G AF +  G H  LV+S   + KECFTTND  F NRPS KAF+ ++YD  SV
Sbjct: 66   LAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMTYDNESV 125

Query: 437  GFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFNELYQLCSTPNNNDATGSN 616
             F PYG  W E+RK+S   LLSN R+Q  K VRA+EV+  F  LY  C  P+        
Sbjct: 126  AFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYDQCKNPS-------- 177

Query: 617  SSYDRDGAVVGRGPVIVEMNKWFEELTLNVVTRMVSGKQHVGTNARHGDSEAKYYKKVID 796
                      G  P++++M KWFEE++ NVV R++ G+Q+ G+    G+ EA +YKKV+D
Sbjct: 178  ----------GSAPILIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMD 227

Query: 797  DAARFMGNHVISDIFPSLGWLDYLLGHDGAMKKTGKELDSIFDSWVKEHQQKRKVQGSSN 976
            +  R     + SD  P LG++D   G+  AMK+  K++D+I ++W++EH++K+    +S 
Sbjct: 228  ELLRLASLSMFSDFAPLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKK----NSV 283

Query: 977  KNHEEDFIDITLSVMEESQLHGLDIETFIKSICVGMIFGGSDTTSVALTWVLSL 1138
               ++DF+D+ LS++EES+L G D +  IK+ C+ MI GG+DTT+V+LTW++SL
Sbjct: 284  AESQQDFMDVMLSIVEESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISL 337


>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
            gi|302142392|emb|CBI19595.3| unnamed protein product
            [Vitis vinifera]
          Length = 527

 Score =  277 bits (709), Expect = 3e-72
 Identities = 160/366 (43%), Positives = 213/366 (58%), Gaps = 3/366 (0%)
 Frame = +2

Query: 50   MDFAAMLLQSALFILSLMVVYTKFWRKSATSNPSSANNKRXXXXXXXXXXXXILGHLHML 229
            MD A  L    +F+  + V Y    RK    +  S +               I+GHLH+L
Sbjct: 1    MDPALHLPAFFVFLSLIYVFYAMLGRKKIIKSSKSRDAPEPGGAWP------IIGHLHLL 54

Query: 230  MG-DELPHHVLGKLADKHGPAFLIHIGPHPELVISSWELAKECFTTNDKYFVNRPSNKAF 406
             G D+L +  LG +ADK+GPAF I +G     V+SSWE+AKECFT NDK    RP+  A 
Sbjct: 55   GGGDQLLYRTLGAMADKYGPAFNIRLGSRRAFVVSSWEVAKECFTINDKALATRPTTVAA 114

Query: 407  KYLSYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFNELYQLCST 586
            K++ Y+ A  GFAPY P W EMRK++   LLSN+R+++ K VR +EVD    ELY L   
Sbjct: 115  KHMGYNYAVFGFAPYSPFWREMRKIATLELLSNRRLEILKHVRTSEVDMGIRELYGLWV- 173

Query: 587  PNNNDATGSNSSYDRDGAVVGRGPVIVEMNKWFEELTLNVVTRMVSGKQHVGTNARHGDS 766
                     NSS           P++VE+N+W E++TLNV+ RMV+GK++ G  A    S
Sbjct: 174  --------KNSS----------RPLLVELNRWLEDMTLNVIVRMVAGKRYFGAAAASDSS 215

Query: 767  EAKYYKKVIDDAARFMGNHVISDIFPSLGWLDYLLGHDGAMKKTGKELDSIFDSWVKEHQ 946
            EA+  +K I    R +G  V+SD  P L WLD L GH+ AMK T KELDSI + W++EH+
Sbjct: 216  EARRCQKAISQFFRLIGIFVVSDALPFLWWLD-LQGHERAMKTTAKELDSILEGWLEEHR 274

Query: 947  QKRKVQGSSNKNHEEDFIDITLSVMEESQLHGL--DIETFIKSICVGMIFGGSDTTSVAL 1120
            Q R+V        E+DFID+ LS+ EE +L G   D ET IKS C+ +I GGSDTT+  L
Sbjct: 275  Q-RRVSSLIKAEGEQDFIDVMLSLQEEGRLSGFQYDSETSIKSTCLALILGGSDTTAGTL 333

Query: 1121 TWVLSL 1138
            TW +SL
Sbjct: 334  TWAISL 339


>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 511

 Score =  275 bits (702), Expect = 2e-71
 Identities = 136/311 (43%), Positives = 203/311 (65%)
 Frame = +2

Query: 206  ILGHLHMLMGDELPHHVLGKLADKHGPAFLIHIGPHPELVISSWELAKECFTTNDKYFVN 385
            I+GHLH+L G EL H  LG +ADK+GP F I IG +  LV+S+ ++AKECFTTNDK F +
Sbjct: 34   IIGHLHLLGGSELRHKTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFAS 93

Query: 386  RPSNKAFKYLSYDQASVGFAPYGPLWIEMRKVSKSNLLSNQRIQMQKQVRATEVDAFFNE 565
            RP++ A K L YD    G APYG  W+E+RK++ S LLSN+R+++ K VR +E+DA   +
Sbjct: 94   RPTSTASKILGYDYVMFGMAPYGQYWVELRKITMSELLSNRRLELLKHVRDSEIDASIQD 153

Query: 566  LYQLCSTPNNNDATGSNSSYDRDGAVVGRGPVIVEMNKWFEELTLNVVTRMVSGKQHVGT 745
            LY++     N+D                +GPV+V+M +WF +LTLNV+ RM++GK++ G+
Sbjct: 154  LYKVW---KNHDK--------------AKGPVLVDMKQWFGDLTLNVILRMIAGKRYSGS 196

Query: 746  NARHGDSEAKYYKKVIDDAARFMGNHVISDIFPSLGWLDYLLGHDGAMKKTGKELDSIFD 925
             +   ++EA+  +K + D  R +G  +I D  P L W D L G+   MK T KELDS+F 
Sbjct: 197  MSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWFD-LQGYKKEMKNTAKELDSVFQ 255

Query: 926  SWVKEHQQKRKVQGSSNKNHEEDFIDITLSVMEESQLHGLDIETFIKSICVGMIFGGSDT 1105
             W++EH + R+   +   N E+DF+D+ +S++E++++   D +T IKS C+ ++ GG DT
Sbjct: 256  RWLEEHNRMRQ---TGELNREQDFMDVLMSILEDTRISEYDNDTIIKSTCLSIVTGGGDT 312

Query: 1106 TSVALTWVLSL 1138
            T V LTW+LSL
Sbjct: 313  TMVTLTWILSL 323


>ref|XP_002284806.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
          Length = 527

 Score =  270 bits (689), Expect = 6e-70
 Identities = 142/335 (42%), Positives = 199/335 (59%)
 Frame = +2

Query: 134  ATSNPSSANNKRXXXXXXXXXXXXILGHLHMLMGDELPHHVLGKLADKHGPAFLIHIGPH 313
            AT   +  N K+            I+GHLH+L  D+LPH  LG +ADK+GP F IH+G  
Sbjct: 25   ATVFNAKGNKKKGKRPQEPSGRWPIIGHLHLLGADKLPHRTLGDMADKYGPIFCIHLGLR 84

Query: 314  PELVISSWELAKECFTTNDKYFVNRPSNKAFKYLSYDQASVGFAPYGPLWIEMRKVSKSN 493
              LV+SSWE+AKEC+TTNDK F  RP + A K + YD A +GFAPYGP W ++RK++   
Sbjct: 85   KALVVSSWEVAKECYTTNDKVFATRPRSLAVKLMGYDHAMLGFAPYGPYWRDVRKLAMVE 144

Query: 494  LLSNQRIQMQKQVRATEVDAFFNELYQLCSTPNNNDATGSNSSYDRDGAVVGRGPVIVEM 673
            LLSN++++M K V+ +EV+    ELY       N D+                 P +VEM
Sbjct: 145  LLSNRQLEMLKHVQDSEVEFLIKELYG--QWARNKDS-----------------PALVEM 185

Query: 674  NKWFEELTLNVVTRMVSGKQHVGTNARHGDSEAKYYKKVIDDAARFMGNHVISDIFPSLG 853
             + F  L +NV+ R ++GK++ GT+A   D E K  KK +DD    +G  ++SD  P LG
Sbjct: 186  KERFGNLVMNVMVRAIAGKRYFGTHA--CDDEPKRGKKALDDFMLLVGLFMVSDAIPFLG 243

Query: 854  WLDYLLGHDGAMKKTGKELDSIFDSWVKEHQQKRKVQGSSNKNHEEDFIDITLSVMEESQ 1033
            WLD + G+   MKK  KELD +   WV+EH+Q+R    ++N   E DF+ + LSV+++ Q
Sbjct: 244  WLDTVKGYTTDMKKIAKELDYLLGRWVEEHRQQR--LSANNNRAEVDFLHVMLSVIDDGQ 301

Query: 1034 LHGLDIETFIKSICVGMIFGGSDTTSVALTWVLSL 1138
              G D +T IK+ C+ +I  G DTTS+ LTW LSL
Sbjct: 302  FSGRDPDTVIKATCLNLILAGYDTTSITLTWALSL 336