BLASTX nr result
ID: Coptis21_contig00005717
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00005717
(1712 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI37009.3| unnamed protein product [Vitis vinifera] 593 e-167
ref|XP_002272765.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe... 588 e-165
ref|XP_002515211.1| conserved hypothetical protein [Ricinus comm... 568 e-159
ref|XP_004158169.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe... 563 e-158
ref|XP_004135966.1| PREDICTED: tRNA guanosine-2'-O-methyltransfe... 563 e-158
>emb|CBI37009.3| unnamed protein product [Vitis vinifera]
Length = 448
Score = 593 bits (1530), Expect = e-167
Identities = 292/452 (64%), Positives = 352/452 (77%), Gaps = 2/452 (0%)
Frame = -3
Query: 1656 RCNFWLPRKNRFCANVPIAHSLFCGNHNPNSEQQRVPCPLDPSHSVLKENMEVHIKRCPF 1477
RC+FWLP+K RFCAN I S+FCGNHN S+ + VPCP+DPSHSVL EN+E H+KRCP
Sbjct: 4 RCSFWLPKKKRFCANFRINDSVFCGNHNTRSDAEWVPCPIDPSHSVLSENLEGHMKRCPL 63
Query: 1476 LKQAETLRLQPYYNKGINGGPDENCSDIHETTSTTISPLMKRNAVYGMSVTELLGLINKI 1297
LKQA++L QP+Y KGIN P + + TS LMKRNAVY M+V E LI+KI
Sbjct: 64 LKQAQSLSSQPFYQKGINAAP--TLASLENATS-----LMKRNAVYSMTVPEFSILISKI 116
Query: 1296 RLIHGSACSDIRDSFKMPEVCSAWMKREVDGKLPFQEKHVLQQASIIGNMDEFGLFSSST 1117
+ IH S +DIRDS+++PE C W+KREVD KLPFQEKHV+QQASI+GN++EFG+ S+
Sbjct: 117 QSIHSSIRNDIRDSYRVPEACDIWIKREVDRKLPFQEKHVVQQASILGNLEEFGVLEKSS 176
Query: 1116 DGVVSKQLEEQQECNGSLRDGNGVSSVVEFGAGRGYLTQMLADCYGINKVFLVERRSYKL 937
EQ + + S D NGV +VVEFGAGRGYLTQMLADCYGI +VFLVER+SYKL
Sbjct: 177 ----GDDQMEQCDSDRSSGDDNGVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKL 232
Query: 936 KADRSLRQKESLILERLRIDIEDLDLSSVESLRGVPYLAVGKHLCGPATDLTLRCCLHAH 757
KADRSLRQKESLILERLRIDIEDL+L +VESL+GVPYLA+GKHLCGPATDL+LRCCL
Sbjct: 233 KADRSLRQKESLILERLRIDIEDLNLKAVESLQGVPYLAIGKHLCGPATDLSLRCCLAEE 292
Query: 756 SNQDRGL--ASDNHLRGLAIATCCHHLCQWKHYINKAYLSELGITKNEFHAITWFTSWAV 583
SNQD + S ++LRGLAIATCCHHLCQWKHYINK YL LGITK++FHAITWFTSWAV
Sbjct: 293 SNQDDAVQCCSGHYLRGLAIATCCHHLCQWKHYINKKYLMNLGITKDDFHAITWFTSWAV 352
Query: 582 DADHSSDISDMVDQGFSVDSIKIKDSGEDACKIEGIVRGIPALERAVLGFMCKDIIDIGR 403
DADH SD+SD+ + SI+ K+ ED + IV+ + A+ERAV+GFMCK+IID+GR
Sbjct: 353 DADHGSDLSDVAGCRLHLQSIEKKECVEDVGGVAEIVQNMKAMERAVVGFMCKEIIDMGR 412
Query: 402 LMWIKKHSLGARLVKYVSSRVSPENHLLVAKY 307
LMW+K+H L +LVKYV +SPENHLL+AK+
Sbjct: 413 LMWVKEHGLETQLVKYVPPTISPENHLLIAKH 444
>ref|XP_002272765.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog [Vitis
vinifera]
Length = 465
Score = 588 bits (1517), Expect = e-165
Identities = 294/462 (63%), Positives = 356/462 (77%), Gaps = 12/462 (2%)
Frame = -3
Query: 1656 RCNFWLPRKNRFCANVPIAHSLFCGNHNPNSEQQRVPCPLDPSHSVLKENMEVHIKRCPF 1477
RC+FWLP+K RFCAN I S+FCGNHN S+ + VPCP+DPSHSVL EN+E H+KRCP
Sbjct: 4 RCSFWLPKKKRFCANFRINDSVFCGNHNTRSDAEWVPCPIDPSHSVLSENLEGHMKRCPL 63
Query: 1476 LKQAETLRLQPYYNKGINGGPD-ENCSDIHETTSTTISPL------MKRNAVYGMSVTEL 1318
LKQA++L QP+Y KGINGG D E+ D + T++ L MKRNAVY M+V E
Sbjct: 64 LKQAQSLSSQPFYQKGINGGKDDEDEGDEKGAAAPTLASLENATSLMKRNAVYSMTVPEF 123
Query: 1317 LGLINKIRLIHGSACSDIRDSFKMPEVCSAWMKREVDGKLPFQEKHVLQQASIIGNMDEF 1138
LI+KI+ IH S +DIRDS+++PE C W+KREVD KLPFQEKHV+QQASI+GN++EF
Sbjct: 124 SILISKIQSIHSSIRNDIRDSYRVPEACDIWIKREVDRKLPFQEKHVVQQASILGNLEEF 183
Query: 1137 GLFSSSTDGVVSKQLEEQQECNGSLRDGNGVSSVVEFGAGRGYLTQMLADCYGINKVFLV 958
G+ S+ EQ + + S D NGV +VVEFGAGRGYLTQMLADCYGI +VFLV
Sbjct: 184 GVLEKSS----GDDQMEQCDSDRSSGDDNGVPAVVEFGAGRGYLTQMLADCYGIKRVFLV 239
Query: 957 ERRSYKLKADRSLRQKESLILERLRIDIEDLDLSSVESLRGVPYLAVGKHLCGPATDLTL 778
ER+SYKLKADRSLRQKESLILERLRIDIEDL+L +VESL+GVPYLA+GKHLCGPATDL+L
Sbjct: 240 ERKSYKLKADRSLRQKESLILERLRIDIEDLNLKAVESLQGVPYLAIGKHLCGPATDLSL 299
Query: 777 RCCLHAHSNQDRGL--ASDNHLRGLAIATCCHHLCQWKHYINKAYLSELGITKNEFHAIT 604
RCCL SNQD + S ++LRGLAIATCCHHLCQWKHYINK YL LGITK++FHAIT
Sbjct: 300 RCCLAEESNQDDAVQCCSGHYLRGLAIATCCHHLCQWKHYINKKYLMNLGITKDDFHAIT 359
Query: 603 WFTSWAVDADHSSDISDMVDQGFSVDSI---KIKDSGEDACKIEGIVRGIPALERAVLGF 433
WFTSWAVDADH SD+SD+ + S + K+ ED + IV+ + A+ERAV+GF
Sbjct: 360 WFTSWAVDADHGSDLSDVAGIDILILSCCHSEKKECVEDVGGVAEIVQNMKAMERAVVGF 419
Query: 432 MCKDIIDIGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAKY 307
MCK+IID+GRLMW+K+H L +LVKYV +SPENHLL+AK+
Sbjct: 420 MCKEIIDMGRLMWVKEHGLETQLVKYVPPTISPENHLLIAKH 461
>ref|XP_002515211.1| conserved hypothetical protein [Ricinus communis]
gi|223545691|gb|EEF47195.1| conserved hypothetical
protein [Ricinus communis]
Length = 438
Score = 568 bits (1463), Expect = e-159
Identities = 281/452 (62%), Positives = 345/452 (76%), Gaps = 2/452 (0%)
Frame = -3
Query: 1656 RCNFWLPRKNRFCANVPIAHSLFCGNHNPNSEQQRVPCPLDPSHSVLKENMEVHIKRCPF 1477
RC FWLP+KNR CAN P++ S FCGNH Q +PCP+D SHSVLKEN+E HIKRCP
Sbjct: 5 RCKFWLPKKNRSCANTPLSDSPFCGNHTQRCNGQWIPCPIDSSHSVLKENLEGHIKRCPL 64
Query: 1476 LKQAETLRLQPYYNKGINGGPDENCSDIHETTSTTISPLMKRNAVYGMSVTELLGLINKI 1297
LKQ ++L LQP+Y KGIN G +++ I+ MKRNAVY M+V+E LI KI
Sbjct: 65 LKQTQSLSLQPFYQKGINAGKEDD---------DDITSEMKRNAVYSMTVSEFYKLIKKI 115
Query: 1296 RLIHGSACSDIRDSFKMPEVCSAWMKREVDGKLPFQEKHVLQQASIIGNMDEFGLFSSST 1117
IH S C+DI +SFK+PE C+ W+KRE+D +LPFQEKHV QQA+I+GN+++FG+ S+
Sbjct: 116 ESIHASLCNDILESFKLPEACNMWIKREIDSQLPFQEKHVKQQAAILGNLEDFGVLKSTL 175
Query: 1116 DGVVSKQLEEQQECNGSLRDGNGVSSVVEFGAGRGYLTQMLADCYGINKVFLVERRSYKL 937
V C+ + N V +VVEFGAGRGYLTQMLADCYGI +VFLVER+SYKL
Sbjct: 176 GNKV---------CDDD--NANCVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKL 224
Query: 936 KADRSLRQKESLILERLRIDIEDLDLSSVESLRGVPYLAVGKHLCGPATDLTLRCCLHAH 757
KADRSLRQKESLILERLRIDIEDL+L++VESL+GVPYLA+GKHLCGPATDLTLRCC
Sbjct: 225 KADRSLRQKESLILERLRIDIEDLNLNAVESLQGVPYLAIGKHLCGPATDLTLRCCFSKQ 284
Query: 756 SNQ-DRGLASDN-HLRGLAIATCCHHLCQWKHYINKAYLSELGITKNEFHAITWFTSWAV 583
S++ + G SDN LRGLAIATCCHHLCQWKHYINK +++LGITK EFHAITWFTSWAV
Sbjct: 285 SSEHNMGHCSDNDFLRGLAIATCCHHLCQWKHYINKNLIADLGITKEEFHAITWFTSWAV 344
Query: 582 DADHSSDISDMVDQGFSVDSIKIKDSGEDACKIEGIVRGIPALERAVLGFMCKDIIDIGR 403
DADH SD+S +D F + S++ + G DA +E VR + A++RA+LGFMCK IID+GR
Sbjct: 345 DADHGSDLS--IDGRFHLQSMEEEQCGGDADGVEDAVRNMKAVQRAILGFMCKQIIDMGR 402
Query: 402 LMWIKKHSLGARLVKYVSSRVSPENHLLVAKY 307
++W K+ L A+LVKYV S VSPENHLL+A++
Sbjct: 403 MIWAKECGLDAKLVKYVPSVVSPENHLLIARH 434
>ref|XP_004158169.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
[Cucumis sativus]
Length = 464
Score = 563 bits (1450), Expect = e-158
Identities = 281/454 (61%), Positives = 337/454 (74%), Gaps = 5/454 (1%)
Frame = -3
Query: 1656 RCNFWLPRKNRFCANVPIAHSLFCGNHNPNSEQQRVPCPLDPSHSVLKENMEVHIKRCPF 1477
RC FWLP+KNRFCA PI S FCGNH+ ++ VPCP+DPSHSVL+EN++ H+KRCP
Sbjct: 4 RCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKRCPL 63
Query: 1476 LKQAETLRLQPYYNKGINGGPDENCSDI--HETTSTTISPLMKRNAVYGMSVTELLGLIN 1303
LKQ ++L QP+Y KGIN G +++ S H S +IS MKRN VYGMS + L+
Sbjct: 64 LKQTQSLAGQPFYQKGINAGEEDSMSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLG 123
Query: 1302 KIRLIHGSACSDIRDSFKMPEVCSAWMKREVDGKLPFQEKHVLQQASIIGNMDEFGLFSS 1123
KIR +H C DI+DS+K+ E C+ W+K E+D K+PFQEKHVLQQASI+GN+DEFG+ S+
Sbjct: 124 KIRALHDLICKDIQDSYKIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSN 183
Query: 1122 STDGVVSKQLEEQQECNGSL-RDGNGVSSVVEFGAGRGYLTQMLADCYGINKVFLVERRS 946
G EQ+E + S D N V +VVEFGAGRGYLTQMLADCYGI +VFLVER+S
Sbjct: 184 YDGG-------EQREGDRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKS 236
Query: 945 YKLKADRSLRQKESLILERLRIDIEDLDLSSVESLRGVPYLAVGKHLCGPATDLTLRCCL 766
YKLKADRSLRQKESLILERLRIDIEDL+L++VESLR PYLA+GKHLCGPATDL LRCCL
Sbjct: 237 YKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCL 296
Query: 765 HAHSNQ--DRGLASDNHLRGLAIATCCHHLCQWKHYINKAYLSELGITKNEFHAITWFTS 592
N + LRGLAIATCCHHLCQW HY NK YL ELGITK EF AITWFTS
Sbjct: 297 TKQPNHAVTERCRDKSKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAITWFTS 356
Query: 591 WAVDADHSSDISDMVDQGFSVDSIKIKDSGEDACKIEGIVRGIPALERAVLGFMCKDIID 412
WAVDA+HS DISD+ D S + + G C ++ IV+ + +ERAVLGFMCK+IID
Sbjct: 357 WAVDANHSEDISDVTDSKTFFQSSENESDGMIGCMVKDIVKSMNPVERAVLGFMCKEIID 416
Query: 411 IGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAK 310
+GRLMW+K+ L +LVKYV S +SPENHLL+AK
Sbjct: 417 MGRLMWLKECGLETQLVKYVPSTISPENHLLIAK 450
>ref|XP_004135966.1| PREDICTED: tRNA guanosine-2'-O-methyltransferase TRM13 homolog
[Cucumis sativus]
Length = 458
Score = 563 bits (1450), Expect = e-158
Identities = 281/454 (61%), Positives = 337/454 (74%), Gaps = 5/454 (1%)
Frame = -3
Query: 1656 RCNFWLPRKNRFCANVPIAHSLFCGNHNPNSEQQRVPCPLDPSHSVLKENMEVHIKRCPF 1477
RC FWLP+KNRFCA PI S FCGNH+ ++ VPCP+DPSHSVL+EN++ H+KRCP
Sbjct: 4 RCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKRCPL 63
Query: 1476 LKQAETLRLQPYYNKGINGGPDENCSDI--HETTSTTISPLMKRNAVYGMSVTELLGLIN 1303
LKQ ++L QP+Y KGIN G +++ S H S +IS MKRN VYGMS + L+
Sbjct: 64 LKQTQSLAGQPFYQKGINAGEEDSMSSNGPHSGLSDSISSEMKRNIVYGMSGDQFHRLLG 123
Query: 1302 KIRLIHGSACSDIRDSFKMPEVCSAWMKREVDGKLPFQEKHVLQQASIIGNMDEFGLFSS 1123
KIR +H C DI+DS+K+ E C+ W+K E+D K+PFQEKHVLQQASI+GN+DEFG+ S+
Sbjct: 124 KIRALHDLICKDIQDSYKIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGVLSN 183
Query: 1122 STDGVVSKQLEEQQECNGSL-RDGNGVSSVVEFGAGRGYLTQMLADCYGINKVFLVERRS 946
G EQ+E + S D N V +VVEFGAGRGYLTQMLADCYGI +VFLVER+S
Sbjct: 184 YDGG-------EQREGDRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKS 236
Query: 945 YKLKADRSLRQKESLILERLRIDIEDLDLSSVESLRGVPYLAVGKHLCGPATDLTLRCCL 766
YKLKADRSLRQKESLILERLRIDIEDL+L++VESLR PYLA+GKHLCGPATDL LRCCL
Sbjct: 237 YKLKADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCL 296
Query: 765 HAHSNQ--DRGLASDNHLRGLAIATCCHHLCQWKHYINKAYLSELGITKNEFHAITWFTS 592
N + LRGLAIATCCHHLCQW HY NK YL ELGITK EF AITWFTS
Sbjct: 297 TKQPNHAVTERCRDKSKLRGLAIATCCHHLCQWNHYTNKRYLLELGITKEEFLAITWFTS 356
Query: 591 WAVDADHSSDISDMVDQGFSVDSIKIKDSGEDACKIEGIVRGIPALERAVLGFMCKDIID 412
WAVDA+HS DISD+ D S + + G C ++ IV+ + +ERAVLGFMCK+IID
Sbjct: 357 WAVDANHSEDISDVTDSKTFFQSSENESDGMIGCMVKDIVKSMNPVERAVLGFMCKEIID 416
Query: 411 IGRLMWIKKHSLGARLVKYVSSRVSPENHLLVAK 310
+GRLMW+K+ L +LVKYV S +SPENHLL+AK
Sbjct: 417 MGRLMWLKECGLETQLVKYVPSTISPENHLLIAK 450