BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00004249
         (2256 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279139.1| PREDICTED: acyl-CoA synthetase family member...   555   0.0  
ref|XP_002298229.1| predicted protein [Populus trichocarpa] gi|2...   504   0.0  
gb|AFD33354.1| acyl-activating enzyme 10 [Cannabis sativa]            497   0.0  
tpg|DAA57519.1| TPA: putative AMP-dependent synthetase and ligas...   479   0.0  
ref|XP_003530154.1| PREDICTED: acyl-CoA synthetase family member...   485   0.0  

>ref|XP_002279139.1| PREDICTED: acyl-CoA synthetase family member 3, mitochondrial-like
            [Vitis vinifera]
          Length = 552

 Score =  555 bits (1430), Expect(2) = 0.0
 Identities = 280/377 (74%), Positives = 314/377 (83%), Gaps = 8/377 (2%)
 Frame = -3

Query: 1903 MEVVKAAFRRGSGACDSVALRLDKESYTYAQXXXXXXXXXXXLCDKVGKTT----SQGNR 1736
            MEV+KA  R+GS   +SVA+R +++SY+Y Q           LC+   K T       + 
Sbjct: 1    MEVIKAVARQGSATAESVAIRANQKSYSYNQLISSARKISSLLCNGDIKPTYGVSKHKHS 60

Query: 1735 GTGHLGGARVGIVAKPSAEFVAGMLGTWLSGGVAVPLALSYPETELLHVMNDSDISMLLS 1556
            G GHLGGAR+GIVAKPS EFVAG+LGTW SGGVAVPLALSYPE ELLHVMNDSD+SM+LS
Sbjct: 61   GNGHLGGARIGIVAKPSVEFVAGILGTWFSGGVAVPLALSYPEAELLHVMNDSDVSMILS 120

Query: 1555 TEDHQELMKNVAAKCSAQFYLLPPVSAISSKTSTH----AGEVDMEKKLSEEIENSKVIE 1388
            TED++ELM+NVAAK SAQF L+PPV +I S TS       GE+  +K L  EI+      
Sbjct: 121  TEDYRELMENVAAKSSAQFSLIPPVPSIPSPTSARDHPQTGEIVADKSLQGEID------ 174

Query: 1387 ADDPALIVYTSGTTGKPKGVVHTHKSINSQVQILTDAWGYSSLDQFLHSLPLHHVHGLFN 1208
             +DPALI+YTSGTTGKPKGVVHTHKSINSQVQILT+AWGY+S DQFLH LPLHHVHGLFN
Sbjct: 175  -EDPALIIYTSGTTGKPKGVVHTHKSINSQVQILTEAWGYTSADQFLHCLPLHHVHGLFN 233

Query: 1207 ALLAPLYAGAVVEFLPKFSVRGIWQRWRESYPKDGTKVDDAITVFTGVPTMYTRLLQGYE 1028
            ALLAPLYAG+ VEF+PKFSVRGIWQRWRES+PKDGTKVDDAITVFTGVPTMYTRL+QGYE
Sbjct: 234  ALLAPLYAGSTVEFMPKFSVRGIWQRWRESHPKDGTKVDDAITVFTGVPTMYTRLIQGYE 293

Query: 1027 AMDPELQATSASAASKLRLMMCGSSALPYPVMEQWENITGHRLLERYGMTEFVMAVSNPL 848
            AMDPELQA SASAASKLRLMMCGSSALPYPVM+QWE ITGHRLLERYGMTEFVMA+SNPL
Sbjct: 294  AMDPELQAASASAASKLRLMMCGSSALPYPVMQQWETITGHRLLERYGMTEFVMAISNPL 353

Query: 847  EGMRKGGTVGKPFPGVQ 797
            +G+RKGGTVGKPFPGVQ
Sbjct: 354  KGVRKGGTVGKPFPGVQ 370



 Score =  291 bits (746), Expect(2) = 0.0
 Identities = 140/172 (81%), Positives = 153/172 (88%)
 Frame = -2

Query: 722 DESGSDIVGVGELCVNSPSLFKEYWKLPEVTKESFIEGGFFKTGDTVKVDEDGYYVILGR 543
           DES ++  GVGELC+ SPSLFKEYWKLPEVTKESFI+GGFFKTGD VKVD+DGYY+ILGR
Sbjct: 376 DESETETTGVGELCIKSPSLFKEYWKLPEVTKESFIDGGFFKTGDAVKVDDDGYYIILGR 435

Query: 542 TSADIMKVGGYKLSALDIEAVLLEHPVVSECCVLGLPDKAYGEAVCTIIVPEESAKKRGE 363
           TSADIMKVGGYKLSAL+IEA LLEHP VSECCVLGLPDK YGEAVC IIVPE   KK+ E
Sbjct: 436 TSADIMKVGGYKLSALEIEAALLEHPAVSECCVLGLPDKDYGEAVCAIIVPEADEKKKQE 495

Query: 362 EEGKPAITLEELQAWAKEKLAPYKIPTRLFLWESLPRNAMGKVNKKELKKVL 207
           EE KPAI+LEEL  WAKEKLAPYK+PT+L LW+ LPRNAMGKVNKKELK+ L
Sbjct: 496 EELKPAISLEELCTWAKEKLAPYKLPTQLLLWDLLPRNAMGKVNKKELKRKL 547


>ref|XP_002298229.1| predicted protein [Populus trichocarpa] gi|222845487|gb|EEE83034.1|
            predicted protein [Populus trichocarpa]
          Length = 539

 Score =  504 bits (1299), Expect(2) = 0.0
 Identities = 252/369 (68%), Positives = 292/369 (79%)
 Frame = -3

Query: 1903 MEVVKAAFRRGSGACDSVALRLDKESYTYAQXXXXXXXXXXXLCDKVGKTTSQGNRGTGH 1724
            MEVVK  +R+G+ A DS+A+R + +SY+Y Q           LC+  G  T  G  G  H
Sbjct: 1    MEVVKKVYRQGTAAADSIAIRAEHKSYSYKQLISSAWEISDLLCNS-GLETVDGVGGCKH 59

Query: 1723 LGGARVGIVAKPSAEFVAGMLGTWLSGGVAVPLALSYPETELLHVMNDSDISMLLSTEDH 1544
            LGGAR+GIVAKPSAEFVAG+L TW SGGVAVPLALSYPETELLHVMND++ISM+LSTEDH
Sbjct: 60   LGGARIGIVAKPSAEFVAGILATWFSGGVAVPLALSYPETELLHVMNDAEISMVLSTEDH 119

Query: 1543 QELMKNVAAKCSAQFYLLPPVSAISSKTSTHAGEVDMEKKLSEEIENSKVIEADDPALIV 1364
            QELM+NVA KC+AQF L+P V  ISS  S H            ++E   + + DDPALI+
Sbjct: 120  QELMQNVADKCAAQFSLIPSVHRISSHESVHD---------HSQVEEIALEKGDDPALII 170

Query: 1363 YTSGTTGKPKGVVHTHKSINSQVQILTDAWGYSSLDQFLHSLPLHHVHGLFNALLAPLYA 1184
            YTSGTTGKPKG VHTHKSI +QVQ L +AW YSS DQFL+ LPLHHVHG FN+L AP+YA
Sbjct: 171  YTSGTTGKPKGAVHTHKSITAQVQTLAEAWEYSSTDQFLNCLPLHHVHGFFNSLFAPIYA 230

Query: 1183 GAVVEFLPKFSVRGIWQRWRESYPKDGTKVDDAITVFTGVPTMYTRLLQGYEAMDPELQA 1004
            G+ VEF+PKFSVRGIWQRWRESYP +G+K DDAITVFTGVPT+Y+RL+QGY AMD + QA
Sbjct: 231  GSTVEFMPKFSVRGIWQRWRESYPPNGSKADDAITVFTGVPTIYSRLIQGYGAMDSDQQA 290

Query: 1003 TSASAASKLRLMMCGSSALPYPVMEQWENITGHRLLERYGMTEFVMAVSNPLEGMRKGGT 824
             SASAA +LRLMM GSSALP PVM++WE ITGHRLLERYGMTEFVMA+SNPL G+RK GT
Sbjct: 291  ASASAARQLRLMMSGSSALPLPVMQEWEAITGHRLLERYGMTEFVMAISNPLRGVRKAGT 350

Query: 823  VGKPFPGVQ 797
            VGKPFPGVQ
Sbjct: 351  VGKPFPGVQ 359



 Score =  288 bits (736), Expect(2) = 0.0
 Identities = 132/173 (76%), Positives = 156/173 (90%)
 Frame = -2

Query: 725 DDESGSDIVGVGELCVNSPSLFKEYWKLPEVTKESFIEGGFFKTGDTVKVDEDGYYVILG 546
           +D++ +D  GVGELC+ SPS+FKEYWKLP+VT++SF + GFF+TGD  K+DEDGYYVILG
Sbjct: 364 EDQNENDTTGVGELCIRSPSMFKEYWKLPQVTEDSFTDDGFFRTGDAGKIDEDGYYVILG 423

Query: 545 RTSADIMKVGGYKLSALDIEAVLLEHPVVSECCVLGLPDKAYGEAVCTIIVPEESAKKRG 366
           RTSADIMKVGGYKLSAL+IE+ LLEHPVV+ECCVLGLPDK YG+AVC IIVPE++ K++ 
Sbjct: 424 RTSADIMKVGGYKLSALEIESALLEHPVVAECCVLGLPDKTYGDAVCAIIVPEDAVKRKQ 483

Query: 365 EEEGKPAITLEELQAWAKEKLAPYKIPTRLFLWESLPRNAMGKVNKKELKKVL 207
           EE  KPAI+LEEL+ WAK+KLAPYK+PTRLFLWESLP NAMGKVNKKELKKVL
Sbjct: 484 EESSKPAISLEELREWAKDKLAPYKLPTRLFLWESLPHNAMGKVNKKELKKVL 536


>gb|AFD33354.1| acyl-activating enzyme 10 [Cannabis sativa]
          Length = 564

 Score =  497 bits (1280), Expect(2) = 0.0
 Identities = 257/386 (66%), Positives = 296/386 (76%), Gaps = 17/386 (4%)
 Frame = -3

Query: 1903 MEVVKAAFRRGSGACDSVALRLDKESYTYAQXXXXXXXXXXXLCD-------KVGK---- 1757
            MEV+K   + GS A + VA+R D++SY+Y Q           LC        + GK    
Sbjct: 1    MEVLKEVAKEGSAAREGVAIRADQKSYSYKQLISSAQSICSLLCGTELKAIHEAGKQARP 60

Query: 1756 -TTSQGNRGTGHLGGARVGIVAKPSAEFVAGMLGTWLSGGVAVPLALSYPETELLHVMND 1580
              +  G  G GHLGGAR+GIVAKPSAEFVAG+LGTWLSGGVAVPLALSYPE ELLHVMND
Sbjct: 61   SASINGAGGHGHLGGARIGIVAKPSAEFVAGVLGTWLSGGVAVPLALSYPEAELLHVMND 120

Query: 1579 SDISMLLSTEDHQELMKNVAAKCSAQFYLLPPVSAISSKTSTHAGEVDMEKKLSEEIENS 1400
            SDISM+LSTEDHQELM+N+AAK SAQF L+P V +  S+      EV ++ + + +I   
Sbjct: 121  SDISMILSTEDHQELMQNIAAKTSAQFSLIPSVPSSCSQ------EVAVDHRQTGDISTD 174

Query: 1399 KV-----IEADDPALIVYTSGTTGKPKGVVHTHKSINSQVQILTDAWGYSSLDQFLHSLP 1235
             +     I  ++PALIVYTSGTTGKPKGVVHTH+SI++QVQ L  AW Y+  DQFLH LP
Sbjct: 175  SILLNREISNENPALIVYTSGTTGKPKGVVHTHQSISAQVQTLAKAWEYTPADQFLHCLP 234

Query: 1234 LHHVHGLFNALLAPLYAGAVVEFLPKFSVRGIWQRWRESYPKDGTKVDDAITVFTGVPTM 1055
            LHHVHGLFNAL APLYA + VEFLPKFSVRGIWQRWRESYP   TK +D ITVFTGVPTM
Sbjct: 235  LHHVHGLFNALFAPLYARSTVEFLPKFSVRGIWQRWRESYPTSETKANDCITVFTGVPTM 294

Query: 1054 YTRLLQGYEAMDPELQATSASAASKLRLMMCGSSALPYPVMEQWENITGHRLLERYGMTE 875
            YTRL+QGYEAMDPEL+  SASAA +LRLMMCGSSALP PVM+QW+ ITGHRLLERYGMTE
Sbjct: 295  YTRLIQGYEAMDPELKEASASAAKQLRLMMCGSSALPVPVMQQWQTITGHRLLERYGMTE 354

Query: 874  FVMAVSNPLEGMRKGGTVGKPFPGVQ 797
            FVMA+SNPL+G RK GTVGKPFPGV+
Sbjct: 355  FVMAISNPLKGERKSGTVGKPFPGVE 380



 Score =  286 bits (731), Expect(2) = 0.0
 Identities = 138/173 (79%), Positives = 151/173 (87%)
 Frame = -2

Query: 725 DDESGSDIVGVGELCVNSPSLFKEYWKLPEVTKESFIEGGFFKTGDTVKVDEDGYYVILG 546
           +DE+G D  GVGELCV SPSLFKEYW+LPEVTK SF + GFFKTGD  KVDEDGYYVILG
Sbjct: 386 EDENGDDATGVGELCVRSPSLFKEYWRLPEVTKASFTDDGFFKTGDAGKVDEDGYYVILG 445

Query: 545 RTSADIMKVGGYKLSALDIEAVLLEHPVVSECCVLGLPDKAYGEAVCTIIVPEESAKKRG 366
           RTSADIMKVGGYKLSAL+IE+VLLEHP V ECCVLGLPDK YGEAV  IIVP   AKK+ 
Sbjct: 446 RTSADIMKVGGYKLSALEIESVLLEHPTVEECCVLGLPDKDYGEAVSAIIVPAAEAKKKR 505

Query: 365 EEEGKPAITLEELQAWAKEKLAPYKIPTRLFLWESLPRNAMGKVNKKELKKVL 207
           EEE +PAI+LEEL +WA+ KLAPYK+PTRLFLW+SLPRNAMGKVNKKELKK L
Sbjct: 506 EEESRPAISLEELFSWAQHKLAPYKLPTRLFLWDSLPRNAMGKVNKKELKKKL 558


>tpg|DAA57519.1| TPA: putative AMP-dependent synthetase and ligase superfamily protein
            [Zea mays] gi|414880390|tpg|DAA57521.1| TPA: putative
            AMP-dependent synthetase and ligase superfamily protein
            [Zea mays]
          Length = 627

 Score =  479 bits (1232), Expect(2) = 0.0
 Identities = 241/380 (63%), Positives = 289/380 (76%), Gaps = 10/380 (2%)
 Frame = -3

Query: 1906 FMEVVKAAFRRGSGACDSVALRLDKESYTYAQXXXXXXXXXXXLCDK------VGKTTSQ 1745
            FMEVV+   R GS       +R +K+SY+  Q           LC K      +  ++S+
Sbjct: 69   FMEVVQEVLRHGSADSVRAVIRANKKSYSLVQLIAASFDVYNILCSKNMTPNGMQDSSSK 128

Query: 1744 GNRGTGHLGGARVGIVAKPSAEFVAGMLGTWLSGGVAVPLALSYPETELLHVMNDSDISM 1565
            G  GTG L GARVGIVAKPS EFVAG+ GTWLSGGVAVPLALSYPE ELLHVMNDSDISM
Sbjct: 129  GRNGTGFLHGARVGIVAKPSPEFVAGVFGTWLSGGVAVPLALSYPEAELLHVMNDSDISM 188

Query: 1564 LLSTEDHQELMKNVAAKCSAQFYLLPPVSAISSKTSTHAGE----VDMEKKLSEEIENSK 1397
            +LST++H E+M+N++ KCSA   +LP V++I S+ +         +     L  E  +SK
Sbjct: 189  VLSTKEHHEIMENLSIKCSAYCSILPSVTSIPSEINPQEPSSNEAISSVSTLITETNSSK 248

Query: 1396 VIEADDPALIVYTSGTTGKPKGVVHTHKSINSQVQILTDAWGYSSLDQFLHSLPLHHVHG 1217
             I+ DDPALI+YTSGTTGKPKGVVHTH+ I SQVQIL++AWGY S DQFLH LPLHHVHG
Sbjct: 249  KIKGDDPALILYTSGTTGKPKGVVHTHEGILSQVQILSEAWGYRSEDQFLHCLPLHHVHG 308

Query: 1216 LFNALLAPLYAGAVVEFLPKFSVRGIWQRWRESYPKDGTKVDDAITVFTGVPTMYTRLLQ 1037
            LFNAL APLY+G+VVEF+PKFSV GIWQRWRESYP D +K  +AITVFTGVPTMYTRLLQ
Sbjct: 309  LFNALFAPLYSGSVVEFIPKFSVSGIWQRWRESYPNDASKSTEAITVFTGVPTMYTRLLQ 368

Query: 1036 GYEAMDPELQATSASAASKLRLMMCGSSALPYPVMEQWENITGHRLLERYGMTEFVMAVS 857
            GY++MDP+ ++ S+ AA  LRLMMCGSSALP P+M++WE +TGHRLLERYGMTEFVMA+S
Sbjct: 369  GYDSMDPDQKSASSYAAKHLRLMMCGSSALPSPLMKRWEEVTGHRLLERYGMTEFVMALS 428

Query: 856  NPLEGMRKGGTVGKPFPGVQ 797
            NPL G+RK GTVGKP P ++
Sbjct: 429  NPLHGIRKEGTVGKPLPRIE 448



 Score =  273 bits (699), Expect(2) = 0.0
 Identities = 127/172 (73%), Positives = 149/172 (86%)
 Frame = -2

Query: 722 DESGSDIVGVGELCVNSPSLFKEYWKLPEVTKESFIEGGFFKTGDTVKVDEDGYYVILGR 543
           ++      GVGELC+ SPSLFKEYWK PEVT ESFI+GGFFKTGDTV VDE+GY++ILGR
Sbjct: 454 EDGAETTTGVGELCIRSPSLFKEYWKRPEVTAESFIDGGFFKTGDTVTVDEEGYFIILGR 513

Query: 542 TSADIMKVGGYKLSALDIEAVLLEHPVVSECCVLGLPDKAYGEAVCTIIVPEESAKKRGE 363
           T+ADIMK GGYKLSAL+IEAVLLEH  V EC VLGLPD+AYGE +C IIVP+E AKK+  
Sbjct: 514 TNADIMKFGGYKLSALEIEAVLLEHDAVLECAVLGLPDEAYGEVICAIIVPKEDAKKKAG 573

Query: 362 EEGKPAITLEELQAWAKEKLAPYKIPTRLFLWESLPRNAMGKVNKKELKKVL 207
           ++ KPA+TLE L +W+K+KLAPYKIPTRL+LW+SLPRNAMGKVNKKELKK+L
Sbjct: 574 QDSKPALTLEALTSWSKDKLAPYKIPTRLYLWDSLPRNAMGKVNKKELKKLL 625


>ref|XP_003530154.1| PREDICTED: acyl-CoA synthetase family member 3, mitochondrial-like
            [Glycine max]
          Length = 606

 Score =  485 bits (1249), Expect(2) = 0.0
 Identities = 247/373 (66%), Positives = 291/373 (78%), Gaps = 4/373 (1%)
 Frame = -3

Query: 1903 MEVVKAAFRRGSGACDSVALRLDKESYTYAQXXXXXXXXXXXLCDKVGKTTSQGNRGTGH 1724
            MEVVKA  +    A +SVA+R D++SY+Y Q              K+       +  TG+
Sbjct: 64   MEVVKAIAKHEPAAPESVAIRADQKSYSYKQLITS--------AQKISNLLCGSDAQTGN 115

Query: 1723 LGGARVGIVAKPSAEFVAGMLGTWLSGGVAVPLALSYPETELLHVMNDSDISMLLSTEDH 1544
            LGGAR+GIVAKPSAEFVAG+LG WLSGGVAVPLA SYPE ELL+V+N+SD+S +LSTEDH
Sbjct: 116  LGGARIGIVAKPSAEFVAGILGIWLSGGVAVPLATSYPEVELLYVINNSDVSAILSTEDH 175

Query: 1543 QELMKNVAAKCSAQFYLLPPVSAISSKTS----THAGEVDMEKKLSEEIENSKVIEADDP 1376
             E+M++VA K S+QF+ LPPV   SS+ S    +  G +  +K L ++   S    ++DP
Sbjct: 176  TEIMQSVANKSSSQFFHLPPVLNKSSEKSRDKHSQNGGIHTDKILLDKFGRS----SEDP 231

Query: 1375 ALIVYTSGTTGKPKGVVHTHKSINSQVQILTDAWGYSSLDQFLHSLPLHHVHGLFNALLA 1196
            ALI+YTSGTTGKPKGVVHTH+SI SQVQ LT AW YSS DQFLH LPLHHVHGLFN L+A
Sbjct: 232  ALILYTSGTTGKPKGVVHTHRSIISQVQTLTKAWEYSSADQFLHCLPLHHVHGLFNGLMA 291

Query: 1195 PLYAGAVVEFLPKFSVRGIWQRWRESYPKDGTKVDDAITVFTGVPTMYTRLLQGYEAMDP 1016
            PLYAG+ VEFLPKFSVRG+WQRWRESYP DG+K ++AITVFTGVPT+Y RL+QGY AMDP
Sbjct: 292  PLYAGSTVEFLPKFSVRGVWQRWRESYPTDGSKAEEAITVFTGVPTIYARLIQGYHAMDP 351

Query: 1015 ELQATSASAASKLRLMMCGSSALPYPVMEQWENITGHRLLERYGMTEFVMAVSNPLEGMR 836
            ELQA S SAA  LRLMMCGSSALP PVM++WE ITGHRLLERYGMTEFVMA+SNPL+G R
Sbjct: 352  ELQAASVSAAKNLRLMMCGSSALPLPVMQEWEAITGHRLLERYGMTEFVMALSNPLKGER 411

Query: 835  KGGTVGKPFPGVQ 797
            K GTVGKPFPG+Q
Sbjct: 412  KPGTVGKPFPGIQ 424



 Score =  266 bits (679), Expect(2) = 0.0
 Identities = 121/173 (69%), Positives = 147/173 (84%)
 Frame = -2

Query: 725 DDESGSDIVGVGELCVNSPSLFKEYWKLPEVTKESFIEGGFFKTGDTVKVDEDGYYVILG 546
           D+ES +   G+GELC+ SPSLFKEYWKLPEVTKESF + GFFKTGD V  DEDGY++ILG
Sbjct: 430 DEESVNGNTGMGELCIKSPSLFKEYWKLPEVTKESFTDDGFFKTGDAVTTDEDGYFIILG 489

Query: 545 RTSADIMKVGGYKLSALDIEAVLLEHPVVSECCVLGLPDKAYGEAVCTIIVPEESAKKRG 366
           RT+ADI+K GGYKLSAL+IE+V++EHP VSECCVLGLPDK YGE V  I+VPE   K++ 
Sbjct: 490 RTNADIIKAGGYKLSALEIESVIIEHPAVSECCVLGLPDKDYGEIVSAIVVPEADVKRKQ 549

Query: 365 EEEGKPAITLEELQAWAKEKLAPYKIPTRLFLWESLPRNAMGKVNKKELKKVL 207
           ++E KP ++LEEL  WAK+K+APYKIPT+L +W+ LPRNAMGKVNKKELKK+L
Sbjct: 550 DQESKPVLSLEELSNWAKDKIAPYKIPTQLIVWDKLPRNAMGKVNKKELKKLL 602