BLASTX nr result
ID: Coptis21_contig00003944
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00003944
(1467 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285921.1| PREDICTED: 4-coumarate--CoA ligase-like 5-li... 265 2e-68
ref|XP_003538157.1| PREDICTED: 4-coumarate--CoA ligase-like 7-li... 265 3e-68
gb|AAF79611.1|AC027665_12 F5M15.17 [Arabidopsis thaliana] 264 5e-68
ref|NP_973872.1| OPC-8:0 CoA ligase1 [Arabidopsis thaliana] gi|3... 264 5e-68
gb|AAP03021.1| 4-coumarate-CoA ligase-like protein [Arabidopsis ... 264 5e-68
>ref|XP_002285921.1| PREDICTED: 4-coumarate--CoA ligase-like 5-like [Vitis vinifera]
Length = 549
Score = 265 bits (677), Expect = 2e-68
Identities = 159/417 (38%), Positives = 237/417 (56%), Gaps = 17/417 (4%)
Frame = +3
Query: 264 EMEVSIESGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKS 443
E+ + SGFC S ++ SK LPQ + + +F+ S+ K AFID+ + +
Sbjct: 6 ELMIDPRSGFCKSNSIFYSKRRPLSLPQNEFIDVATFISSRPHQ---GKTAFIDAATGRR 62
Query: 444 ITYGELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHT 623
+T+ ++ S++ LGI+KGD++L++ PNS+++P++ L V+SLGA+ +T NPL+T
Sbjct: 63 LTFTDVWRAVDSVSSCLSELGIRKGDVILLLTPNSIFFPIVCLSVLSLGAVVTTTNPLNT 122
Query: 624 RPELQSQIQDTNPKLIITTPEQRSKLDDRQSARPIILVDD------------LMDTLLQY 767
E+ QI D+ P L+ TT KL S PI+L+ + +D +++
Sbjct: 123 PREIGKQIVDSKPVLVFTTQSLAPKLTG--SNLPIVLMGEHGDYHAGAEVVGSLDEMIRK 180
Query: 768 PRQHKTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAM--SCFLRHVWESEDGAFD 941
K +G +NQ+D A L+YSSGTTG+SK VV HRN IAM S + +E E+ +F
Sbjct: 181 EPNGKRVGERVNQEDAATLLYSSGTTGESKGVVSSHRNLIAMVQSIVSMNSFEDEEESF- 239
Query: 942 DIYLCVLPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXX 1121
+C LP+FHM+GL F G LA ST +VL ++ + +ML ++ KY+ T
Sbjct: 240 ---VCFLPMFHMYGLGAFALGRLARGSTVIVLPRFEMNEMLTTISKYRATCLPLVPPILV 296
Query: 1122 XXXXXXDGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTE--TCGPI 1292
D +K YDL S++ +IC GAPL K +E+F + YP + + Q Y LTE T G
Sbjct: 297 ALVNGADQIKAKYDLSSLQYIICGGAPLSKEVIEEFLEKYPNVNIRQGYALTESTTVGAS 356
Query: 1293 TLCNGFSGMVHESIGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDP 1463
T S H + G L PSMEAKIVD +GK+L GEL LRGP++M+GYF +P
Sbjct: 357 THTLEESRR-HGTAGLLSPSMEAKIVDPESGKALSVNRAGELWLRGPTIMKGYFRNP 412
>ref|XP_003538157.1| PREDICTED: 4-coumarate--CoA ligase-like 7-like [Glycine max]
Length = 548
Score = 265 bits (676), Expect = 3e-68
Identities = 158/405 (39%), Positives = 234/405 (57%), Gaps = 19/405 (4%)
Frame = +3
Query: 306 GLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITYGELRSLTSSLA 485
G+Y S +LP P+LSL SFL +SS K A +DS S +++T L+S + LA
Sbjct: 10 GIYRSLRPCLVLPNDPNLSLVSFLFQSVSSFP-SKIALVDSHSSQTLTLAHLKSQVAKLA 68
Query: 486 HVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPELQSQIQDTNPK 665
H F LGI K D+VL++ PNS++YP+ L ++GA+ STANP++T E+ Q+ D+NPK
Sbjct: 69 HGFLKLGINKNDVVLLLAPNSIHYPICFLAATAIGAVVSTANPIYTVNEISKQVDDSNPK 128
Query: 666 LIITTPEQRSKLD---------DRQSARPIILVD--------DLMDTLLQYPRQHKTL-G 791
L+IT PE K+ D ++A+ ++ + +D +++ L
Sbjct: 129 LLITVPELWDKVKNLNLPAVIIDTETAQGLVSFEAGNEVSRITSLDAVMEMAGPATELPE 188
Query: 792 TEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCVLPLF 971
+ + Q D AAL+YSSGTTG SK VV HRNFIA S + + + G DD+YLCVLP+F
Sbjct: 189 SGVKQGDTAALLYSSGTTGLSKGVVLTHRNFIAASVMI-GMDDDLAGEQDDVYLCVLPMF 247
Query: 972 HMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXXDGVK 1151
H+FGL++ L S VV+ ++ LE +L ++EK +VT V
Sbjct: 248 HVFGLAVVTYAALRRGSAVVVMERFELEALLKAIEKQRVT-KLWVVPPILLGLAKQSVVG 306
Query: 1152 GYDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSGMVHE- 1328
YDL S++ + APLGK ME+ + +P + + Q YG+TETCG +++ N G+ H
Sbjct: 307 NYDLSSLRRIGSGAAPLGKDLMEECGRRFPHVAICQGYGMTETCGIVSVENPRVGVRHTG 366
Query: 1329 SIGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDP 1463
S G L+ +EA+IV V T K LPP + GE+ +RGP++MQGY +P
Sbjct: 367 STGTLVSGVEAQIVSVDTQKPLPPRQLGEIWVRGPNMMQGYHNNP 411
>gb|AAF79611.1|AC027665_12 F5M15.17 [Arabidopsis thaliana]
Length = 580
Score = 264 bits (674), Expect = 5e-68
Identities = 156/413 (37%), Positives = 238/413 (57%), Gaps = 17/413 (4%)
Frame = +3
Query: 273 VSIESGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITY 452
V+ SGFC+S + SK LP P L + +F+ SQ + AFID+ + +++T+
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRI---AFIDASTGQNLTF 60
Query: 453 GELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPE 632
EL S+A +GI+KG +VL++ PNS+ +P++ L VMSLGAI +T NPL+T E
Sbjct: 61 TELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 120
Query: 633 LQSQIQDTNPKLIITTPEQRSKLDDRQSARPIILVDD-----------LMDTLLQYPRQH 779
+ QI+D+NP L TT + K+ PI+L+D+ L++ + + P +
Sbjct: 121 IAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGN 180
Query: 780 KTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 959
+ + ++QDD A L+YSSGTTG SK V+ HRN IAM + + + S+DG + ++C
Sbjct: 181 R-VKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDG--EQRFICT 237
Query: 960 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXX 1139
+P+FH++GL+ F G LA ST +VL+K+ + +M+ ++ KY+ T
Sbjct: 238 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 297
Query: 1140 DGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSG 1316
D +K YDL S+ V+C GAPL K E F++ YP +++ Q YGLTE+ G +
Sbjct: 298 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG----IGASTD 353
Query: 1317 MVHES-----IGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGD 1460
V ES G L SME +IVD TG+ L P + GEL L+GPS+M+GYF +
Sbjct: 354 TVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSN 406
>ref|NP_973872.1| OPC-8:0 CoA ligase1 [Arabidopsis thaliana]
gi|332191860|gb|AEE29981.1| OPC-8:0 CoA ligase1
[Arabidopsis thaliana]
Length = 473
Score = 264 bits (674), Expect = 5e-68
Identities = 156/413 (37%), Positives = 238/413 (57%), Gaps = 17/413 (4%)
Frame = +3
Query: 273 VSIESGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITY 452
V+ SGFC+S + SK LP P L + +F+ SQ + AFID+ + +++T+
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRI---AFIDASTGQNLTF 60
Query: 453 GELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPE 632
EL S+A +GI+KG +VL++ PNS+ +P++ L VMSLGAI +T NPL+T E
Sbjct: 61 TELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 120
Query: 633 LQSQIQDTNPKLIITTPEQRSKLDDRQSARPIILVDD-----------LMDTLLQYPRQH 779
+ QI+D+NP L TT + K+ PI+L+D+ L++ + + P +
Sbjct: 121 IAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGN 180
Query: 780 KTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 959
+ + ++QDD A L+YSSGTTG SK V+ HRN IAM + + + S+DG + ++C
Sbjct: 181 R-VKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDG--EQRFICT 237
Query: 960 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXX 1139
+P+FH++GL+ F G LA ST +VL+K+ + +M+ ++ KY+ T
Sbjct: 238 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 297
Query: 1140 DGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSG 1316
D +K YDL S+ V+C GAPL K E F++ YP +++ Q YGLTE+ G +
Sbjct: 298 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG----IGASTD 353
Query: 1317 MVHES-----IGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGD 1460
V ES G L SME +IVD TG+ L P + GEL L+GPS+M+GYF +
Sbjct: 354 TVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSN 406
>gb|AAP03021.1| 4-coumarate-CoA ligase-like protein [Arabidopsis thaliana]
Length = 546
Score = 264 bits (674), Expect = 5e-68
Identities = 156/413 (37%), Positives = 238/413 (57%), Gaps = 17/413 (4%)
Frame = +3
Query: 273 VSIESGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITY 452
V+ SGFC+S + SK LP P L + +F+ SQ + AFID+ + +++T+
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRI---AFIDASTGQNLTF 60
Query: 453 GELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPE 632
EL S+A +GI+KG +VL++ PNS+ +P++ L VMSLGAI +T NPL+T E
Sbjct: 61 TELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 120
Query: 633 LQSQIQDTNPKLIITTPEQRSKLDDRQSARPIILVDD-----------LMDTLLQYPRQH 779
+ QI+D+NP L TT + K+ PI+L+D+ L++ + + P +
Sbjct: 121 IAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGN 180
Query: 780 KTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 959
+ + ++QDD A L+YSSGTTG SK V+ HRN IAM + + + S+DG + ++C
Sbjct: 181 R-VKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDG--EQRFICT 237
Query: 960 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXX 1139
+P+FH++GL+ F G LA ST +VL+K+ + +M+ ++ KY+ T
Sbjct: 238 VPMFHIYGLTAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 297
Query: 1140 DGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSG 1316
D +K YDL S+ V+C GAPL K E F++ YP +++ Q YGLTE+ G +
Sbjct: 298 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG----IGASTD 353
Query: 1317 MVHES-----IGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGD 1460
V ES G L SME +IVD TG+ L P + GEL L+GPS+M+GYF +
Sbjct: 354 TVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSN 406