BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00003235
         (1499 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003602643.1| E3 ubiquitin-protein ligase mib1 [Medicago t...   559   e-157
ref|XP_003525130.1| PREDICTED: E3 ubiquitin-protein ligase RGLG1...   556   e-156
ref|XP_002309925.1| predicted protein [Populus trichocarpa] gi|1...   555   e-156
ref|XP_002306262.1| predicted protein [Populus trichocarpa] gi|2...   553   e-155
ref|XP_003523218.1| PREDICTED: E3 ubiquitin-protein ligase RGLG1...   548   e-153

>ref|XP_003602643.1| E3 ubiquitin-protein ligase mib1 [Medicago truncatula]
            gi|355491691|gb|AES72894.1| E3 ubiquitin-protein ligase
            mib1 [Medicago truncatula]
          Length = 432

 Score =  559 bits (1440), Expect = e-157
 Identities = 278/394 (70%), Positives = 315/394 (79%), Gaps = 4/394 (1%)
 Frame = -3

Query: 1401 THQQPSFQAQSSMDTHHRQDPR--TSDMDPRNRNKHQPQRIDDNYSSLDQVISALRDAGL 1228
            T+ QPS    +  +T+H Q     TS ++ R+ +  QP  I DN+SSLDQV+SALR+AGL
Sbjct: 39   TYDQPSAYTGNLGNTNHHQPSSHATSSVNTRHHHNKQPTYIADNFSSLDQVVSALREAGL 98

Query: 1227 ESSNLILGIDFTKSNEWTGKYSFNRRSLHAIGSTPNPYEQAISIIGRTLSPFDEDNLIPC 1048
            ESSNLILGIDFTKSNEWTGKYSF+R+SLH IGS PNPYEQAISIIGRTLS FDEDNLIPC
Sbjct: 99   ESSNLILGIDFTKSNEWTGKYSFHRKSLHHIGSAPNPYEQAISIIGRTLSTFDEDNLIPC 158

Query: 1047 FGFGDASTHDKFVFRFFAENRYCHGFEEVLFRYREIVPHLKLSGPTSFAPVIDAAIDIVE 868
            FGFGDASTHD+ VF F+ +NR CHGFEEVL RYR+IVPHLKLSGPTSFAPVIDAAIDIVE
Sbjct: 159  FGFGDASTHDQNVFSFYPDNRVCHGFEEVLARYRQIVPHLKLSGPTSFAPVIDAAIDIVE 218

Query: 867  KSSGQYHVLVIIADGQVTRNPDTPPGRVSHQEQATINSIVAASHYPLSIILVGVGDGPWD 688
            +++GQYHVLVIIADGQVTRNP TP G++S QEQATI SI+AASHYPLSIILVGVGDGPWD
Sbjct: 219  RNNGQYHVLVIIADGQVTRNPGTPHGKLSPQEQATITSIIAASHYPLSIILVGVGDGPWD 278

Query: 687  AMHQFDDNIPYRAFDNFQFVNFTKIMSENKDTSKKESEFALAALMEIPIQYKATQRLSIL 508
             M  FDDNI  R FDNFQFVNFTKIMSEN + SKKE+EFALAALMEIP QY+A Q + + 
Sbjct: 279  EMKHFDDNITGRVFDNFQFVNFTKIMSENTEASKKETEFALAALMEIPFQYRAAQNIQLA 338

Query: 507  ESDNVGLPRTSXXXXXPEVIDHDNAVNMHPPMTSFQPVVDMAP--VEQVCPICLTNPKDM 334
             S+ V            E+IDHDNA    P +T+F  +   AP   E VCPICLTNPKDM
Sbjct: 339  NSEPVHYQHKRPLPPPKEIIDHDNAFIATPRITNFDSIEPTAPASAEPVCPICLTNPKDM 398

Query: 333  AFGCGHLTCKECSITVSTCPICREPITTRLRLYS 232
            AFGCGH TCKEC  T+S CP+CR+ ITTRLRLY+
Sbjct: 399  AFGCGHTTCKECGATLSLCPMCRQQITTRLRLYT 432


>ref|XP_003525130.1| PREDICTED: E3 ubiquitin-protein ligase RGLG1-like [Glycine max]
          Length = 426

 Score =  556 bits (1433), Expect = e-156
 Identities = 286/426 (67%), Positives = 325/426 (76%), Gaps = 18/426 (4%)
 Frame = -3

Query: 1455 MGNTESMDDMDD----APRY-----VNT----HQQPSFQAQSSMDTHHRQDPRT---SDM 1324
            MGN ES  D  D     PR       NT    HQ PS+   S+  +H    P +   S +
Sbjct: 1    MGNEESTHDSHDDFFRQPRSHDGSSPNTSHQYHQPPSYDGSSANTSHQYHQPSSYAGSSV 60

Query: 1323 DPRNRNKHQPQRIDDNYSSLDQVISALRDAGLESSNLILGIDFTKSNEWTGKYSFNRRSL 1144
            + R++ K +P  I D++SSLDQV+SALR+AGLESSNLI+GIDFTKSNEWTGK+SFN +SL
Sbjct: 61   NTRHQYKQRPTYIADSFSSLDQVVSALREAGLESSNLIIGIDFTKSNEWTGKHSFNHKSL 120

Query: 1143 HAIGSTPNPYEQAISIIGRTLSPFDEDNLIPCFGFGDASTHDKFVFRFFAENRYCHGFEE 964
            H  G+TPNPYEQAISIIGRTLS FDEDNLIPCFGFGDASTHD+ VF F+ +  YCHGFE+
Sbjct: 121  HFTGNTPNPYEQAISIIGRTLSSFDEDNLIPCFGFGDASTHDQNVFSFYPDECYCHGFEQ 180

Query: 963  VLFRYREIVPHLKLSGPTSFAPVIDAAIDIVEKSSGQYHVLVIIADGQVTRNPDTPPGRV 784
            VL RYREIVPHLKL+GPTSFAPVIDAA+ IVE+S+GQYHVLVIIADGQVTRN DTP G+ 
Sbjct: 181  VLARYREIVPHLKLAGPTSFAPVIDAAVGIVERSNGQYHVLVIIADGQVTRNSDTPHGKF 240

Query: 783  SHQEQATINSIVAASHYPLSIILVGVGDGPWDAMHQFDDNIPYRAFDNFQFVNFTKIMSE 604
            S QEQATINSI+AASHYPLSIILVGVGDGPWD M  FDDNI  R FDNFQFVNFTKI SE
Sbjct: 241  SPQEQATINSIIAASHYPLSIILVGVGDGPWDEMQHFDDNITQRLFDNFQFVNFTKITSE 300

Query: 603  NKDTSKKESEFALAALMEIPIQYKATQRLSILESDNVGLPRTSXXXXXPEVIDHDNAVNM 424
            NKD SKKE+ FALAALMEIPIQY+ TQ L I   +     R        EVID DNAV  
Sbjct: 301  NKDASKKEAAFALAALMEIPIQYRITQNLQIANENPTSHQRKRPLPPPKEVIDRDNAVLA 360

Query: 423  HPPMTSFQPVVDMAP--VEQVCPICLTNPKDMAFGCGHLTCKECSITVSTCPICREPITT 250
             P + +F+ V   AP  VE VCPICLTNPKDMAFGCGH TCKEC +T+S+CP+CR+ ITT
Sbjct: 361  VPHVPNFESVEPSAPAAVESVCPICLTNPKDMAFGCGHTTCKECGVTLSSCPMCRQEITT 420

Query: 249  RLRLYS 232
            RLRLY+
Sbjct: 421  RLRLYT 426


>ref|XP_002309925.1| predicted protein [Populus trichocarpa] gi|118486339|gb|ABK95010.1|
            unknown [Populus trichocarpa] gi|222852828|gb|EEE90375.1|
            predicted protein [Populus trichocarpa]
          Length = 364

 Score =  555 bits (1431), Expect = e-156
 Identities = 273/365 (74%), Positives = 309/365 (84%)
 Frame = -3

Query: 1326 MDPRNRNKHQPQRIDDNYSSLDQVISALRDAGLESSNLILGIDFTKSNEWTGKYSFNRRS 1147
            MD   + +  P  I DN++SLDQVISALR+AGLESSNLILGIDFTKSNEWTG++SF+R+S
Sbjct: 1    MDHNYQRRQHPTYIADNFNSLDQVISALREAGLESSNLILGIDFTKSNEWTGRHSFHRKS 60

Query: 1146 LHAIGSTPNPYEQAISIIGRTLSPFDEDNLIPCFGFGDASTHDKFVFRFFAENRYCHGFE 967
            LHAIGST NPYEQAISIIGRTLSPFDEDNLIPCFGFGDASTHDK+VF F+ +NR C+  E
Sbjct: 61   LHAIGSTLNPYEQAISIIGRTLSPFDEDNLIPCFGFGDASTHDKYVFSFYPDNRPCNDLE 120

Query: 966  EVLFRYREIVPHLKLSGPTSFAPVIDAAIDIVEKSSGQYHVLVIIADGQVTRNPDTPPGR 787
            E L RYREIVPHLKLSGPTSFA +I+AAIDIVEKS GQYHVLVIIADGQVTR PDTPPGR
Sbjct: 121  EALARYREIVPHLKLSGPTSFAAIINAAIDIVEKS-GQYHVLVIIADGQVTRTPDTPPGR 179

Query: 786  VSHQEQATINSIVAASHYPLSIILVGVGDGPWDAMHQFDDNIPYRAFDNFQFVNFTKIMS 607
            +S QEQAT+NSIVAAS +PLSII VGVGDGPW+AM QFDDNIP R FDNFQFVNFTKIM 
Sbjct: 180  LSPQEQATVNSIVAASKHPLSIIFVGVGDGPWNAMQQFDDNIPQREFDNFQFVNFTKIML 239

Query: 606  ENKDTSKKESEFALAALMEIPIQYKATQRLSILESDNVGLPRTSXXXXXPEVIDHDNAVN 427
            +N + SKKE+ FALAALMEIP+QY+ATQRL   + D VG PRT       EVIDHDNAV 
Sbjct: 240  DNTEASKKETAFALAALMEIPLQYRATQRLQHTDHDLVGGPRTRPLPPPREVIDHDNAVK 299

Query: 426  MHPPMTSFQPVVDMAPVEQVCPICLTNPKDMAFGCGHLTCKECSITVSTCPICREPITTR 247
              P +T+F+ V     VE VCPICLTNPKDMAFGCGHLTC++C  ++STCPICR+PITTR
Sbjct: 300  SIPHITNFETVQPSTSVEPVCPICLTNPKDMAFGCGHLTCRDCGASLSTCPICRQPITTR 359

Query: 246  LRLYS 232
            L+L++
Sbjct: 360  LKLFT 364


>ref|XP_002306262.1| predicted protein [Populus trichocarpa] gi|222855711|gb|EEE93258.1|
            predicted protein [Populus trichocarpa]
          Length = 351

 Score =  553 bits (1424), Expect = e-155
 Identities = 270/355 (76%), Positives = 303/355 (85%), Gaps = 3/355 (0%)
 Frame = -3

Query: 1287 IDDNYSSLDQVISALRDAGLESSNLILGIDFTKSNEWTGKYSFNRRSLHAIGSTPNPYEQ 1108
            I DN++SLDQVIS+LR+AGLESSNLILGIDFTKSNEWTG++SF+R+SLHAIGST NPYEQ
Sbjct: 3    IADNFNSLDQVISSLREAGLESSNLILGIDFTKSNEWTGRHSFHRKSLHAIGSTLNPYEQ 62

Query: 1107 AISIIGRTLSPFDEDNLIPCFGFGDASTHDKFVFRFFAENRYCHGFEEVLFRYREIVPHL 928
            AISIIGRTLSPFDEDNLIPCFGFGDASTHDK VF F+ +NR C+G EE L RYREIVPHL
Sbjct: 63   AISIIGRTLSPFDEDNLIPCFGFGDASTHDKHVFSFYPDNRPCNGLEEALTRYREIVPHL 122

Query: 927  KLSGPTSFAPVIDAAIDIVEKSSGQYHVLVIIADGQVTRNPDTPPGRVSHQEQATINSIV 748
            KLSGPTSFAP+IDAAIDIVEKS GQYHVLV+IADGQVTR PDTPPGR S QE AT+NSIV
Sbjct: 123  KLSGPTSFAPIIDAAIDIVEKSGGQYHVLVVIADGQVTRGPDTPPGRFSPQEHATVNSIV 182

Query: 747  AASHYPLSIILVGVGDGPWDAMHQFDDNIPYRAFDNFQFVNFTKIMSENKDTSKKESEFA 568
            AAS YP+SIILVGVGDGPWDAM QFDDNIP RAFDNFQFVNFTKIMS+N + SKKE+ FA
Sbjct: 183  AASKYPISIILVGVGDGPWDAMQQFDDNIPQRAFDNFQFVNFTKIMSDNTEASKKETAFA 242

Query: 567  LAALMEIPIQYKATQRLSILESDNVGLPRTSXXXXXPEVIDHDNAVNMHPPMTSFQPVVD 388
            LAALMEIP+QY+ATQ L +      G P T       EVIDHDNAV   P MT+F+PV  
Sbjct: 243  LAALMEIPLQYRATQTLQL------GGPHTRLLPPPREVIDHDNAVKSIPHMTNFKPVQP 296

Query: 387  MAP---VEQVCPICLTNPKDMAFGCGHLTCKECSITVSTCPICREPITTRLRLYS 232
             AP   VE VCPICL NPKD+AFGCGH+TC++C  ++S CP+CR+PITTRLRL++
Sbjct: 297  TAPTASVEPVCPICLANPKDLAFGCGHMTCRDCGASISACPLCRQPITTRLRLFT 351


>ref|XP_003523218.1| PREDICTED: E3 ubiquitin-protein ligase RGLG1-like [Glycine max]
          Length = 432

 Score =  548 bits (1411), Expect = e-153
 Identities = 278/432 (64%), Positives = 328/432 (75%), Gaps = 24/432 (5%)
 Frame = -3

Query: 1455 MGNTESMDDMDD-----APRYVNTHQQPSFQAQSSMDTHHRQDPRT-------------- 1333
            MGN+ESM +  D      P Y N++ QPS  A++S +T + +                  
Sbjct: 1    MGNSESMYESQDNFYQHPPSYYNSYHQPSPYAENSENTTYHEPSSYAWNSSNTTYYQSST 60

Query: 1332 ---SDMDPRNRNKHQPQRIDDNYSSLDQVISALRDAGLESSNLILGIDFTKSNEWTGKYS 1162
               S  + R+++  QP  I DN+SSLDQV+S+LR+AGLESSNLILGIDFTKSNEWTGK+S
Sbjct: 61   YAGSSENTRHQHTRQPTYIADNFSSLDQVVSSLREAGLESSNLILGIDFTKSNEWTGKHS 120

Query: 1161 FNRRSLHAIGSTPNPYEQAISIIGRTLSPFDEDNLIPCFGFGDASTHDKFVFRFFAENRY 982
            F+R+SLH IG+TPNPYEQAISI+G TLS FDEDNLIPCFGFGDASTHD+ VF F+ +NR+
Sbjct: 121  FHRKSLHHIGNTPNPYEQAISIVGHTLSSFDEDNLIPCFGFGDASTHDQNVFCFYQDNRF 180

Query: 981  CHGFEEVLFRYREIVPHLKLSGPTSFAPVIDAAIDIVEKSSGQYHVLVIIADGQVTRNPD 802
            CHGFEEVL RYREIVP++KLSGPTSFAPVIDAAIDIVE+++GQYHVLVIIADGQVTRNPD
Sbjct: 181  CHGFEEVLARYREIVPYIKLSGPTSFAPVIDAAIDIVERNNGQYHVLVIIADGQVTRNPD 240

Query: 801  TPPGRVSHQEQATINSIVAASHYPLSIILVGVGDGPWDAMHQFDDNIPYRAFDNFQFVNF 622
             P G++S QEQATINSI+AASHYPLSIILVGVGDGPWD M  +DDNI  R FDNFQFVNF
Sbjct: 241  VPYGKLSPQEQATINSIIAASHYPLSIILVGVGDGPWDEMKYYDDNITERLFDNFQFVNF 300

Query: 621  TKIMSENKDTSKKESEFALAALMEIPIQYKATQRLSILESDNVGLPRTSXXXXXPEVIDH 442
            TKIMSEN + SKKE+ FALAALMEIP+QY+A Q + + + ++V            EVI H
Sbjct: 301  TKIMSENTEASKKEATFALAALMEIPLQYRAAQNIQLNDRESVLHQHKRPLPPPNEVIHH 360

Query: 441  DNAVNMHPPMTSFQPVVDMAP--VEQVCPICLTNPKDMAFGCGHLTCKECSITVSTCPIC 268
            DNA    P M + +     AP   E VCPICLTNPKDMAFGCGH TCKEC  T+S+CP+C
Sbjct: 361  DNARMAIPHMPNLESAESTAPAAAEPVCPICLTNPKDMAFGCGHTTCKECGSTLSSCPMC 420

Query: 267  REPITTRLRLYS 232
            R  ITTRLRLY+
Sbjct: 421  RHQITTRLRLYT 432