BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00002902
         (2250 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI23872.3| unnamed protein product [Vitis vinifera]              614   e-173
ref|XP_002276384.1| PREDICTED: 3-isopropylmalate dehydrogenase, ...   614   e-173
gb|ACF17658.1| putative 3-isopropylmalate dehydrogenase large su...   610   e-172
sp|P29102.1|LEU3_BRANA RecName: Full=3-isopropylmalate dehydroge...   603   e-170
ref|XP_002298230.1| predicted protein [Populus trichocarpa] gi|2...   603   e-170

>emb|CBI23872.3| unnamed protein product [Vitis vinifera]
          Length = 506

 Score =  614 bits (1584), Expect = e-173
 Identities = 316/389 (81%), Positives = 342/389 (87%)
 Frame = +2

Query: 701  IRCSASSKRREYNITLLPGDGIGPEVISVAKKVLTLAGSQQGIEFQFKEMPVGGAALDLV 880
            IRCSA++  + Y+ITLLPGDGIGPEVISVAK VL LAGS +GIEF F+EMPVGG ALDL 
Sbjct: 133  IRCSAATPPKRYSITLLPGDGIGPEVISVAKNVLKLAGSLEGIEFGFQEMPVGGCALDLT 192

Query: 881  GVPLPDETLSVAKHSHAVLLGAIGGXXXXXXXXXXXXXXXXXXXXXXXXXYKWDNNEKHL 1060
            GVPLP+ETL VAK S AVLLGAIGG                         YKWD+NEKHL
Sbjct: 193  GVPLPEETLLVAKQSDAVLLGAIGG-------------------------YKWDSNEKHL 227

Query: 1061 KPETGLLQLRAGLGVFANLRPATVLPQLVDSSTLKKEVAEGVDLMVVRELTGGIYFGHPR 1240
            KPETGLLQLR+GL VFANLRPA+VLPQLVD+STLKKEVAEGVDLMVVRELTGGIYFG PR
Sbjct: 228  KPETGLLQLRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPR 287

Query: 1241 GFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRV 1420
            GFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRV
Sbjct: 288  GFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRV 347

Query: 1421 TMMASDYPDVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPS 1600
            T MAS+YPDVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSIGMLPS
Sbjct: 348  TAMASEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPS 407

Query: 1601 ASLGESGPGLFEPIHGSAPDISGQDKANPLATVLSVAMLLRYGLHEENAARIIESAVLDT 1780
            AS+GESGPGLFEPIHGSAPDI+GQDKANPLATVLS AMLLRYGL E NAA+ IE+AVLDT
Sbjct: 408  ASVGESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAKRIETAVLDT 467

Query: 1781 LNKGFRTGDIFSAGKKLVGCKEIGEEVMK 1867
            L++GFRT DI+ AG KLVGCKE+GE+V+K
Sbjct: 468  LDRGFRTRDIYEAGTKLVGCKEMGEQVLK 496


>ref|XP_002276384.1| PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic [Vitis
            vinifera]
          Length = 405

 Score =  614 bits (1584), Expect = e-173
 Identities = 316/389 (81%), Positives = 342/389 (87%)
 Frame = +2

Query: 701  IRCSASSKRREYNITLLPGDGIGPEVISVAKKVLTLAGSQQGIEFQFKEMPVGGAALDLV 880
            IRCSA++  + Y+ITLLPGDGIGPEVISVAK VL LAGS +GIEF F+EMPVGG ALDL 
Sbjct: 32   IRCSAATPPKRYSITLLPGDGIGPEVISVAKNVLKLAGSLEGIEFGFQEMPVGGCALDLT 91

Query: 881  GVPLPDETLSVAKHSHAVLLGAIGGXXXXXXXXXXXXXXXXXXXXXXXXXYKWDNNEKHL 1060
            GVPLP+ETL VAK S AVLLGAIGG                         YKWD+NEKHL
Sbjct: 92   GVPLPEETLLVAKQSDAVLLGAIGG-------------------------YKWDSNEKHL 126

Query: 1061 KPETGLLQLRAGLGVFANLRPATVLPQLVDSSTLKKEVAEGVDLMVVRELTGGIYFGHPR 1240
            KPETGLLQLR+GL VFANLRPA+VLPQLVD+STLKKEVAEGVDLMVVRELTGGIYFG PR
Sbjct: 127  KPETGLLQLRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPR 186

Query: 1241 GFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRV 1420
            GFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRV
Sbjct: 187  GFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLWRKRV 246

Query: 1421 TMMASDYPDVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPS 1600
            T MAS+YPDVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSIGMLPS
Sbjct: 247  TAMASEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPS 306

Query: 1601 ASLGESGPGLFEPIHGSAPDISGQDKANPLATVLSVAMLLRYGLHEENAARIIESAVLDT 1780
            AS+GESGPGLFEPIHGSAPDI+GQDKANPLATVLS AMLLRYGL E NAA+ IE+AVLDT
Sbjct: 307  ASVGESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAKRIETAVLDT 366

Query: 1781 LNKGFRTGDIFSAGKKLVGCKEIGEEVMK 1867
            L++GFRT DI+ AG KLVGCKE+GE+V+K
Sbjct: 367  LDRGFRTRDIYEAGTKLVGCKEMGEQVLK 395


>gb|ACF17658.1| putative 3-isopropylmalate dehydrogenase large subunit [Capsicum
            annuum]
          Length = 406

 Score =  610 bits (1572), Expect = e-172
 Identities = 316/413 (76%), Positives = 343/413 (83%)
 Frame = +2

Query: 629  NPLKFNTTIPIHIKSVTPLTKWSRIRCSASSKRREYNITLLPGDGIGPEVISVAKKVLTL 808
            +PL  +  +P H        KW  IRCSA+++ + YNITLLPGDGIGPEVISVAK  L L
Sbjct: 15   DPLFHSQKLPKHA------AKWGTIRCSATTQSKSYNITLLPGDGIGPEVISVAKNALQL 68

Query: 809  AGSQQGIEFQFKEMPVGGAALDLVGVPLPDETLSVAKHSHAVLLGAIGGXXXXXXXXXXX 988
              S +G E  F+EMP+GGAALD VGVPLPDETL  AK S A LLGAIGG           
Sbjct: 69   VASLEGFEVAFEEMPMGGAALDAVGVPLPDETLKSAKQSDATLLGAIGG----------- 117

Query: 989  XXXXXXXXXXXXXXYKWDNNEKHLKPETGLLQLRAGLGVFANLRPATVLPQLVDSSTLKK 1168
                          YKWDNN KHLKPETGLLQLR GL VFANLRPATVLPQLVD+STLKK
Sbjct: 118  --------------YKWDNNAKHLKPETGLLQLRKGLQVFANLRPATVLPQLVDASTLKK 163

Query: 1169 EVAEGVDLMVVRELTGGIYFGHPRGFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQK 1348
            EVAEGVDLMVVRELTGGIYFG PRGFS +ENGEEIGFNTEVYATYEIDRIARIAFETA+K
Sbjct: 164  EVAEGVDLMVVRELTGGIYFGKPRGFSTDENGEEIGFNTEVYATYEIDRIARIAFETARK 223

Query: 1349 RRGKLCSVDKANVLEASMLWRKRVTMMASDYPDVELTHMYVDNAAMQLIRDPKQFDTIVT 1528
            RRGKLCSVDKANVLEASMLWRKRVT +AS+YPDVEL+HMYVDNAAMQL+R+PKQFDTIVT
Sbjct: 224  RRGKLCSVDKANVLEASMLWRKRVTALASEYPDVELSHMYVDNAAMQLVRNPKQFDTIVT 283

Query: 1529 NNIFGDILSDEASMLTGSIGMLPSASLGESGPGLFEPIHGSAPDISGQDKANPLATVLSV 1708
            NNIFGDILSDEASM+TGSIGMLPSASLG SGPGLFEPIHGSAPDI+GQDKANPLAT+LS 
Sbjct: 284  NNIFGDILSDEASMITGSIGMLPSASLGASGPGLFEPIHGSAPDIAGQDKANPLATMLSA 343

Query: 1709 AMLLRYGLHEENAARIIESAVLDTLNKGFRTGDIFSAGKKLVGCKEIGEEVMK 1867
            AMLL+YGL EE AAR IE+AVLD+LN+GFRTGDI SAG KLVGCKE+GEEV+K
Sbjct: 344  AMLLKYGLGEEKAARRIEAAVLDSLNRGFRTGDIHSAGHKLVGCKEMGEEVLK 396


>sp|P29102.1|LEU3_BRANA RecName: Full=3-isopropylmalate dehydrogenase, chloroplastic;
            Short=3-IPM-DH; Short=IMDH; AltName: Full=Beta-IPM
            dehydrogenase; Flags: Precursor gi|17827|emb|CAA42596.1|
            3-isopropylmalate dehydrogenase [Brassica napus]
          Length = 406

 Score =  603 bits (1556), Expect = e-170
 Identities = 311/423 (73%), Positives = 350/423 (82%), Gaps = 1/423 (0%)
 Frame = +2

Query: 602  MSTLNMINNNPLKFNTTIPIHIKSVTPLTKWSRIRCSASSK-RREYNITLLPGDGIGPEV 778
            M+     N  P+KF  T+    K  +P     R+RC+A+S  ++ YNITLLPGDGIGPEV
Sbjct: 1    MAAALQTNIRPVKFPATLRALTKQSSPAP--FRVRCAAASPGKKRYNITLLPGDGIGPEV 58

Query: 779  ISVAKKVLTLAGSQQGIEFQFKEMPVGGAALDLVGVPLPDETLSVAKHSHAVLLGAIGGX 958
            IS+AK VL  AGS +G+EF F+EMPVGGAALDLVGVPLP+ET+S AK S AVLLGAIGG 
Sbjct: 59   ISIAKNVLQQAGSLEGLEFSFQEMPVGGAALDLVGVPLPEETVSAAKESDAVLLGAIGG- 117

Query: 959  XXXXXXXXXXXXXXXXXXXXXXXXYKWDNNEKHLKPETGLLQLRAGLGVFANLRPATVLP 1138
                                    YKWD NEKHLKPETGLLQLRAGL VFANLRPATVLP
Sbjct: 118  ------------------------YKWDKNEKHLKPETGLLQLRAGLKVFANLRPATVLP 153

Query: 1139 QLVDSSTLKKEVAEGVDLMVVRELTGGIYFGHPRGFSKNENGEEIGFNTEVYATYEIDRI 1318
            QLVD+STLK+EVAEGVDLMVVRELTGGIYFG PRG   NENGEE+G+NTEVYA +EIDRI
Sbjct: 154  QLVDASTLKREVAEGVDLMVVRELTGGIYFGVPRGIKTNENGEEVGYNTEVYAAHEIDRI 213

Query: 1319 ARIAFETAQKRRGKLCSVDKANVLEASMLWRKRVTMMASDYPDVELTHMYVDNAAMQLIR 1498
            AR+AFETA+KRRGKLCSVDKANVL+AS+LWR+RVT +A++YPDVEL+HMYVDNAAMQL+R
Sbjct: 214  ARVAFETARKRRGKLCSVDKANVLDASILWRRRVTALAAEYPDVELSHMYVDNAAMQLVR 273

Query: 1499 DPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPSASLGESGPGLFEPIHGSAPDISGQDK 1678
            DPKQFDTIVTNNIFGDILSDEASM+TGSIGMLPSASL +SGPGLFEPIHGSAPDI+GQDK
Sbjct: 274  DPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDK 333

Query: 1679 ANPLATVLSVAMLLRYGLHEENAARIIESAVLDTLNKGFRTGDIFSAGKKLVGCKEIGEE 1858
            ANPLAT+LS AMLL+YGL EE AA+ IE AVL  LNKGFRTGDI+SAG KLVGCKE+GEE
Sbjct: 334  ANPLATILSAAMLLKYGLGEEKAAKRIEDAVLGALNKGFRTGDIYSAGTKLVGCKEMGEE 393

Query: 1859 VMK 1867
            V+K
Sbjct: 394  VLK 396


>ref|XP_002298230.1| predicted protein [Populus trichocarpa] gi|222845488|gb|EEE83035.1|
            predicted protein [Populus trichocarpa]
          Length = 400

 Score =  603 bits (1554), Expect = e-170
 Identities = 308/393 (78%), Positives = 339/393 (86%), Gaps = 2/393 (0%)
 Frame = +2

Query: 695  SRIRCSASSKR--REYNITLLPGDGIGPEVISVAKKVLTLAGSQQGIEFQFKEMPVGGAA 868
            SRIRC+A++ +  + Y+ITLLPGDGIGPEVISVAK VL LAGS +GIEF F+EMP+GG+A
Sbjct: 23   SRIRCAAATTKQSKRYSITLLPGDGIGPEVISVAKNVLNLAGSLEGIEFNFQEMPMGGSA 82

Query: 869  LDLVGVPLPDETLSVAKHSHAVLLGAIGGXXXXXXXXXXXXXXXXXXXXXXXXXYKWDNN 1048
            LD+ GVPLPDETL  AK S AVLLGAIGG                         YKWD N
Sbjct: 83   LDMAGVPLPDETLLAAKKSDAVLLGAIGG-------------------------YKWDKN 117

Query: 1049 EKHLKPETGLLQLRAGLGVFANLRPATVLPQLVDSSTLKKEVAEGVDLMVVRELTGGIYF 1228
            EKHLKPETGLLQLR GL VFANLRPATVLPQLVD+STLKKEVAEGVD+MVVRELTGGIYF
Sbjct: 118  EKHLKPETGLLQLREGLEVFANLRPATVLPQLVDASTLKKEVAEGVDVMVVRELTGGIYF 177

Query: 1229 GHPRGFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEASMLW 1408
            G PRGF  NE+GEEIGFNTEVYATYE+DRIAR+AFETA+KR GKLCSVDKANVLEASM W
Sbjct: 178  GKPRGFGTNEDGEEIGFNTEVYATYEVDRIARVAFETARKRHGKLCSVDKANVLEASMFW 237

Query: 1409 RKRVTMMASDYPDVELTHMYVDNAAMQLIRDPKQFDTIVTNNIFGDILSDEASMLTGSIG 1588
            RKRV  +A++YPDVEL+HMYVDNAAMQL+R+PKQFDTIVTNNIFGDILSDEASM+TGSIG
Sbjct: 238  RKRVISIAAEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIG 297

Query: 1589 MLPSASLGESGPGLFEPIHGSAPDISGQDKANPLATVLSVAMLLRYGLHEENAARIIESA 1768
            MLPSASLG SGPGLFEPIHGSAPDI+ QDKANPLATVLS AMLL+YGL EENAA+ IE+A
Sbjct: 298  MLPSASLGSSGPGLFEPIHGSAPDIANQDKANPLATVLSAAMLLKYGLGEENAAKRIENA 357

Query: 1769 VLDTLNKGFRTGDIFSAGKKLVGCKEIGEEVMK 1867
            VLDTLNKGFRTGDI+SAG KLVGCKE+GEEV+K
Sbjct: 358  VLDTLNKGFRTGDIYSAGNKLVGCKEMGEEVLK 390