BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00002217
         (1028 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002516294.1| cytochrome P450, putative [Ricinus communis]...   446   e-123
dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       436   e-120
ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ...   436   e-120
ref|XP_003635295.1| PREDICTED: secologanin synthase-like [Vitis ...   435   e-119
ref|XP_002281332.1| PREDICTED: secologanin synthase [Vitis vinif...   431   e-118

>ref|XP_002516294.1| cytochrome P450, putative [Ricinus communis]
            gi|223544780|gb|EEF46296.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 488

 Score =  446 bits (1147), Expect = e-123
 Identities = 210/341 (61%), Positives = 272/341 (79%)
 Frame = +1

Query: 4    RKLISPAFHQEKLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRT 183
            R+LI+PAFH EKLKGM P F+ SC ++IE+WKK    Q  CE+D W +IQ    D+ISR 
Sbjct: 122  RRLINPAFHLEKLKGMVPVFVISCNKMIEEWKKMINHQETCEVDVWPEIQKVAKDIISRA 181

Query: 184  AFGSNFEEGKKIFELQKEQSILVMQAVQSVYIPGFRFIPTKENKRRMNLDKEIVVNLRDL 363
            AFGSN+EEGKKIFELQ E  +L ++A+Q++YIPGFRF+PTK+N+RR  L+ EI   LRD+
Sbjct: 182  AFGSNYEEGKKIFELQHELMMLTIEAMQTLYIPGFRFVPTKKNRRRKKLNIEITSMLRDI 241

Query: 364  IRRKTLALESGKSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQE 543
            + RK  A+ +G+S VDDLL +LL  S ++S  + +  ++ G++I++VIEECK+FY AGQE
Sbjct: 242  VERKQNAMRTGQSKVDDLLSLLLHSSEQNSSGNESGRRSDGLTIEEVIEECKVFYLAGQE 301

Query: 544  TSSNLLTWTMIVLAMHPSWQEKAREDVLQICGKNMPSYEDLNHLKIVTMILYEVLRLYPP 723
            T+S+LLTWT+IVLAMH  WQEKARE+VLQ+CGK  P +E L HLK VTMIL EVLRLYPP
Sbjct: 302  TTSSLLTWTIIVLAMHQDWQEKAREEVLQVCGKKEPDFEALTHLKTVTMILNEVLRLYPP 361

Query: 724  LVQQIRYTCKTTKLGDITLPAGVQLILPILLIHHDREFWGDDAEDFRPERFSEGISKASK 903
             +   ++T + TK+GDI++PAGV + LP +LIH D EFWGDDAE+F+PERF+ GI+KASK
Sbjct: 362  AIALYQHTREATKIGDISIPAGVDITLPTMLIHRDPEFWGDDAEEFKPERFAAGITKASK 421

Query: 904  DQKVAFFPFGWGPRICIGQNFAMIEAKMALAMILQHFSFQL 1026
            D  +AFFPFGWGPRICIGQ+F+++EAK  LAMILQHFSF+L
Sbjct: 422  DH-LAFFPFGWGPRICIGQSFSLLEAKTVLAMILQHFSFEL 461


>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  436 bits (1121), Expect = e-120
 Identities = 213/341 (62%), Positives = 268/341 (78%)
 Frame = +1

Query: 4    RKLISPAFHQEKLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRT 183
            R++++PAFH EKLK M PA  TSC E++ KW+    SQ  CELD W  +Q+ TADVISRT
Sbjct: 154  RRILNPAFHLEKLKMMLPAIYTSCSEMVNKWEMMV-SQDSCELDVWPHLQNLTADVISRT 212

Query: 184  AFGSNFEEGKKIFELQKEQSILVMQAVQSVYIPGFRFIPTKENKRRMNLDKEIVVNLRDL 363
            AFGS++EEG++IF+LQ EQ+ LV++A+QS+YIPG RF+PTK N R   +  E  V LRD+
Sbjct: 213  AFGSSYEEGRRIFQLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDI 272

Query: 364  IRRKTLALESGKSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQE 543
            + ++  A++ G++  DDLLG+L++ S      D  N K  GM+ID+VI+ECKLFYFAGQE
Sbjct: 273  VNKREKAMKIGEAHNDDLLGLLME-SNFKEITDNVNSKNFGMTIDEVIDECKLFYFAGQE 331

Query: 544  TSSNLLTWTMIVLAMHPSWQEKAREDVLQICGKNMPSYEDLNHLKIVTMILYEVLRLYPP 723
            T+S LL WTM+VL+MHP WQEKARE+VLQ+ G   P ++ LNHLKIVTMILYEVLRLYPP
Sbjct: 332  TTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDGLNHLKIVTMILYEVLRLYPP 391

Query: 724  LVQQIRYTCKTTKLGDITLPAGVQLILPILLIHHDREFWGDDAEDFRPERFSEGISKASK 903
             V   R T KT KLG+ITLPAGV L +P LL+HHDRE WG+DAEDF PERFS G+SKA+K
Sbjct: 392  AVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGVSKATK 451

Query: 904  DQKVAFFPFGWGPRICIGQNFAMIEAKMALAMILQHFSFQL 1026
            +Q V+FFPF WGPRICIGQNFA++E+KMA+AMILQ FSF+L
Sbjct: 452  NQ-VSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFEL 491


>ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
            vinifera]
          Length = 520

 Score =  436 bits (1120), Expect = e-120
 Identities = 213/342 (62%), Positives = 271/342 (79%), Gaps = 1/342 (0%)
 Frame = +1

Query: 4    RKLISPAFHQEKLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRT 183
            R++I+PAFH EKLK M PAF TSC  LIE+WK+    Q  CE+D W ++Q+ T DVISR 
Sbjct: 153  RRIINPAFHLEKLKVMIPAFTTSCSMLIERWKELASLQETCEVDIWPELQNLTRDVISRA 212

Query: 184  AFGSNFEEGKKIFELQKEQSILVMQAVQSVYIPGFRFIPTKENKRRMNLDKEIVVNLRDL 363
            A GS+FEEG++IFELQKE   L ++A+Q++YIPGFRFIPTK+N+RR  L K      RDL
Sbjct: 213  ALGSSFEEGRQIFELQKEHITLTLEAMQTLYIPGFRFIPTKKNQRRKYLQKRTTSMFRDL 272

Query: 364  IRRKTLALESGKSSVDDLLGMLLQYSRESSQAD-MNNLKTTGMSIDQVIEECKLFYFAGQ 540
            I+RK  A+ +G++  D+LLG+LL  S +++  + + + K   +++++VIEECK FY AG 
Sbjct: 273  IQRKKDAIRTGQAEGDNLLGLLLLSSSQNNLPENVMSTKDNAITLEEVIEECKQFYLAGH 332

Query: 541  ETSSNLLTWTMIVLAMHPSWQEKAREDVLQICGKNMPSYEDLNHLKIVTMILYEVLRLYP 720
            ET+S+ LTWT+ VLAMHP+WQEKARE+V+QICGK  P  E L+HLKIV+MILYEVLRLYP
Sbjct: 333  ETTSSWLTWTVTVLAMHPNWQEKAREEVMQICGKKEPDSEALSHLKIVSMILYEVLRLYP 392

Query: 721  PLVQQIRYTCKTTKLGDITLPAGVQLILPILLIHHDREFWGDDAEDFRPERFSEGISKAS 900
            P++   ++  K TK+G I+LPAGV L LP LLIHHD E WGDDAE+F+PERF+EG+SKAS
Sbjct: 393  PVIAVYQHAYKETKIGTISLPAGVDLTLPTLLIHHDPELWGDDAEEFKPERFAEGVSKAS 452

Query: 901  KDQKVAFFPFGWGPRICIGQNFAMIEAKMALAMILQHFSFQL 1026
            KDQ +AFFPFGWGPR CIGQNFAMIEAK+ALAMILQHFSF+L
Sbjct: 453  KDQ-LAFFPFGWGPRTCIGQNFAMIEAKVALAMILQHFSFEL 493


>ref|XP_003635295.1| PREDICTED: secologanin synthase-like [Vitis vinifera]
            gi|297741788|emb|CBI33075.3| unnamed protein product
            [Vitis vinifera]
          Length = 513

 Score =  435 bits (1118), Expect = e-119
 Identities = 206/342 (60%), Positives = 264/342 (77%)
 Frame = +1

Query: 1    QRKLISPAFHQEKLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISR 180
            +RKL++PAFH EKLKGM PAF  SC +LIE+WK     QG  ELD   + Q+ T DVIS+
Sbjct: 154  RRKLLTPAFHFEKLKGMVPAFSVSCRKLIERWKNLVAPQGTYELDMMPEFQNLTGDVISQ 213

Query: 181  TAFGSNFEEGKKIFELQKEQSILVMQAVQSVYIPGFRFIPTKENKRRMNLDKEIVVNLRD 360
             AFGSN+EEGKK+FELQKEQ++LVM+A ++ YIPGFRF+P  +NK+R  +D EI   L+ 
Sbjct: 214  VAFGSNYEEGKKVFELQKEQAVLVMEAFRTFYIPGFRFVPIGKNKKRYYIDSEIKAILKK 273

Query: 361  LIRRKTLALESGKSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAGQ 540
            +I ++   ++ G    DDLLG+LLQ   ++            M+I+ VIEECKLFYFAGQ
Sbjct: 274  IILKRKQTMKPGDLGNDDLLGLLLQCQEQTDSE---------MTIEDVIEECKLFYFAGQ 324

Query: 541  ETSSNLLTWTMIVLAMHPSWQEKAREDVLQICGKNMPSYEDLNHLKIVTMILYEVLRLYP 720
            ET++N LTWT+++L+MHP+WQEKARE+VLQICGK MP  E +NHLKIV+MIL+EVLRLYP
Sbjct: 325  ETTANWLTWTILLLSMHPNWQEKAREEVLQICGKKMPDIEAINHLKIVSMILHEVLRLYP 384

Query: 721  PLVQQIRYTCKTTKLGDITLPAGVQLILPILLIHHDREFWGDDAEDFRPERFSEGISKAS 900
            P+ QQ R+TC+   +  + +PAGV L+LP LL+HH  E+WGDD E+F+PERFSEG+SKAS
Sbjct: 385  PVTQQFRHTCERINIAGMCIPAGVNLVLPTLLLHHSPEYWGDDVEEFKPERFSEGVSKAS 444

Query: 901  KDQKVAFFPFGWGPRICIGQNFAMIEAKMALAMILQHFSFQL 1026
            K  ++AF+PFGWG RIC+GQ FAMIEAKMALAMILQHF F+L
Sbjct: 445  KGDQIAFYPFGWGHRICLGQGFAMIEAKMALAMILQHFWFEL 486


>ref|XP_002281332.1| PREDICTED: secologanin synthase [Vitis vinifera]
            gi|296081714|emb|CBI20719.3| unnamed protein product
            [Vitis vinifera]
          Length = 512

 Score =  431 bits (1108), Expect = e-118
 Identities = 208/343 (60%), Positives = 268/343 (78%), Gaps = 1/343 (0%)
 Frame = +1

Query: 1    QRKLISPAFHQEKLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISR 180
            +RKLI+PAFH EKL GM PAF  SC  LIE+WK     QG  ELD   + Q+ T DVISR
Sbjct: 152  RRKLITPAFHVEKLMGMVPAFSMSCCNLIERWKNWVGPQGTYELDVMPEFQNVTGDVISR 211

Query: 181  TAFGSNFEEGKKIFELQKEQSILVMQAVQSVYIPGFRFIPTKENKRRMNLDKEIVVNLRD 360
             AFGS++EEGKK+FELQKEQ++LV++A +++Y+PGFRF+PT +N+RR ++D EI   LR 
Sbjct: 212  AAFGSSYEEGKKVFELQKEQAVLVIEASRAIYLPGFRFVPTVKNRRRYHIDNEIKAMLRS 271

Query: 361  LIRRKTLALESGKSSV-DDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIEECKLFYFAG 537
            +I RK  A+++G S   DDLLG+LLQ + E             M I+ +IEECKLFYFAG
Sbjct: 272  MIDRKKQAMKNGDSGYNDDLLGLLLQLTEEIDNE---------MRIEDLIEECKLFYFAG 322

Query: 538  QETSSNLLTWTMIVLAMHPSWQEKAREDVLQICGKNMPSYEDLNHLKIVTMILYEVLRLY 717
            QET++NLLTWTMI+L+M+P WQ+KARE+VLQICGK +P  E + HLKIV+MIL+EVLRLY
Sbjct: 323  QETTANLLTWTMILLSMNPKWQDKAREEVLQICGKKIPDLEAIKHLKIVSMILHEVLRLY 382

Query: 718  PPLVQQIRYTCKTTKLGDITLPAGVQLILPILLIHHDREFWGDDAEDFRPERFSEGISKA 897
            P +V  +RYT K T +  +++PAGV+L LP +L+HH  E+WGDD E+F+PERFSEG+SKA
Sbjct: 383  PSVVNLLRYTHKRTDVAGLSIPAGVELYLPTILLHHSPEYWGDDVEEFKPERFSEGVSKA 442

Query: 898  SKDQKVAFFPFGWGPRICIGQNFAMIEAKMALAMILQHFSFQL 1026
            SK  ++AF+PFGWGPRIC+GQ+FAMIEAKMALAMILQ+F F+L
Sbjct: 443  SKGDQIAFYPFGWGPRICLGQSFAMIEAKMALAMILQNFWFEL 485