BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00001961
         (1735 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEK01241.1| ent-kaurene oxidase [Pyrus communis]                   692   0.0  
gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]              690   0.0  
gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]                   689   0.0  
ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplasti...   687   0.0  
ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus commu...   683   0.0  

>gb|AEK01241.1| ent-kaurene oxidase [Pyrus communis]
          Length = 514

 Score =  692 bits (1787), Expect = 0.0
 Identities = 320/466 (68%), Positives = 399/466 (85%)
 Frame = +1

Query: 160  PSLPVVPGLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAM 339
            PS+PVVPGLP++GNLLQLK+KKP++TFTRWAE YGPIYSI+TGA+T+VVLNT DVAKEAM
Sbjct: 48   PSVPVVPGLPVVGNLLQLKEKKPYKTFTRWAEEYGPIYSIRTGATTMVVLNTADVAKEAM 107

Query: 340  VTRFPSISTRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRD 519
            VTR+ S+STRKLS ALKILT DKCMVATSDYN+FH   KRY+L+  LGANAQKR R  RD
Sbjct: 108  VTRYSSMSTRKLSKALKILTFDKCMVATSDYNDFHNMVKRYVLTNVLGANAQKRHRSQRD 167

Query: 520  TMIKNMVRTLHSYTKDRPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSR 699
            TM +N+   LH++  + P +A NFR +FE+ELFG+ALKQ +GKD+E  + VEELGTTLSR
Sbjct: 168  TMRENVASRLHAHVNNSPQEAINFREIFESELFGIALKQAIGKDIEDSINVEELGTTLSR 227

Query: 700  EEIFEVLVLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKR 879
            ++IF+VLV D + GAI+VDWRDFFPYLRWVP K+++  ++R+  RR AVM  L+ ++KKR
Sbjct: 228  DDIFKVLVNDMMEGAIEVDWRDFFPYLRWVPIKTIENNMKRLSRRRIAVMNVLIDEQKKR 287

Query: 880  IASGEVLNSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKGHQD 1059
            IASGE L+ ++D+L SE KT + E+++MLIWE IIET+DTTLVTTEW MYELAK++  QD
Sbjct: 288  IASGEELDCHIDYLLSEAKTLTPEQISMLIWETIIETADTTLVTTEWGMYELAKDQKRQD 347

Query: 1060 QLYQEIESVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIP 1239
            +L++E+++VCG++KVTEE++SQ+PYL AVFHETLR+HSP PI+P+RY HEDTELGGY++P
Sbjct: 348  RLFEEVQNVCGTNKVTEEHLSQMPYLGAVFHETLRKHSPAPIVPLRYVHEDTELGGYYVP 407

Query: 1240 AGSEIAVNIYGCNMDKHQWAEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAM 1419
             G+EIA+NIYGCNMDK+QW  PE+WKPERFLD KYD MD+YKTMAFG GKRVCAGS QAM
Sbjct: 408  VGTEIAINIYGCNMDKNQWESPEEWKPERFLDPKYDPMDLYKTMAFGTGKRVCAGSLQAM 467

Query: 1420 TISCIAIATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPLHAIITPR 1557
             I+C  I   VQEFEWKL +GE+E+VDT+GLTTHKLHP+HAI+ PR
Sbjct: 468  LIACTTIGRLVQEFEWKLRDGEEENVDTVGLTTHKLHPMHAILKPR 513


>gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]
          Length = 514

 Score =  690 bits (1780), Expect = 0.0
 Identities = 324/466 (69%), Positives = 408/466 (87%)
 Frame = +1

Query: 160  PSLPVVPGLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAM 339
            P +PVVPGLP++GNLLQLK+KKP++TF RWAE++GPIYSI+TGAST+VV+N+T VAKEAM
Sbjct: 49   PPVPVVPGLPVVGNLLQLKEKKPYKTFLRWAEIHGPIYSIRTGASTMVVVNSTHVAKEAM 108

Query: 340  VTRFPSISTRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRD 519
            VTRF SISTRKLS AL++LTS+K MVATSDYNEFHK  K+YIL+  LGANAQKR R HRD
Sbjct: 109  VTRFSSISTRKLSKALELLTSNKSMVATSDYNEFHKMVKKYILAELLGANAQKRHRIHRD 168

Query: 520  TMIKNMVRTLHSYTKDRPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSR 699
            T+I+N++  LH++TK+ PLQA NFR +FE+ELFGLA+KQ LG D++S ++VEELGTTLSR
Sbjct: 169  TLIENVLNKLHAHTKNSPLQAVNFRKIFESELFGLAMKQALGYDVDS-LFVEELGTTLSR 227

Query: 700  EEIFEVLVLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKR 879
            EEI+ VLV D L GAI+VDWRDFFPYL+W+PNKS +MKI+R+ +RR+AVM ++V ++KK 
Sbjct: 228  EEIYNVLVSDMLKGAIEVDWRDFFPYLKWIPNKSFEMKIQRLASRRQAVMNSIVKEQKKS 287

Query: 880  IASGEVLNSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKGHQD 1059
            IASG+  N Y+++L SE KT +E+++++L WE IIET+DTT+VTTEWAMYELAKN   QD
Sbjct: 288  IASGKGENCYLNYLLSEAKTLTEKQISILAWETIIETADTTVVTTEWAMYELAKNPKQQD 347

Query: 1060 QLYQEIESVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIP 1239
            +LY EI++VCG+DK+TEE++S+LPYLSAVFHETLR++SP P++P+RYAHEDT+LGGY++P
Sbjct: 348  RLYNEIQNVCGTDKITEEHLSKLPYLSAVFHETLRKYSPSPLVPLRYAHEDTQLGGYYVP 407

Query: 1240 AGSEIAVNIYGCNMDKHQWAEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAM 1419
            AG+EIAVNIYGCNMDK+QW  PE+WKPERFLD KYD MDMYKTM+FG GKRVCAGS QA 
Sbjct: 408  AGTEIAVNIYGCNMDKNQWETPEEWKPERFLDEKYDPMDMYKTMSFGSGKRVCAGSLQAS 467

Query: 1420 TISCIAIATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPLHAIITPR 1557
             I+C +I   VQEFEW+L +GE E+VDTLGLTTHKL+P+ AI+ PR
Sbjct: 468  LIACTSIGRLVQEFEWRLKDGEVENVDTLGLTTHKLYPMQAILQPR 513


>gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]
          Length = 508

 Score =  689 bits (1778), Expect = 0.0
 Identities = 327/467 (70%), Positives = 400/467 (85%)
 Frame = +1

Query: 160  PSLPVVPGLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAM 339
            P +P +PGLPLIGNLLQLK+KKPH+TF RWAE YGPI+SI+TGAST++VLN+++VAKEAM
Sbjct: 43   PPVPDIPGLPLIGNLLQLKEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAM 102

Query: 340  VTRFPSISTRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRD 519
            VTRF SISTRKLSNALKILT DKCMVATSDYN+FHK  K +IL   LGA AQKR R HRD
Sbjct: 103  VTRFSSISTRKLSNALKILTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRD 162

Query: 520  TMIKNMVRTLHSYTKDRPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSR 699
            T+I+N+ + LH++ K  PL+    + +FE+E+FGLALKQ LGKD+ES +YVEELGTTLSR
Sbjct: 163  TLIENISKYLHAHVKTSPLEPVVLKKIFESEIFGLALKQALGKDIES-IYVEELGTTLSR 221

Query: 700  EEIFEVLVLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKR 879
            EEIF VLV+DP+ GAI+VDWRDFFPYL W+PNKS++MKI+RM  RR A+MKAL+G++KKR
Sbjct: 222  EEIFAVLVVDPMAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKR 281

Query: 880  IASGEVLNSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKGHQD 1059
            I SGE  NSY+DFL SE  T +E+++ MLIWE IIE SDTTLVT+EWAMYELAK+   Q+
Sbjct: 282  IGSGEEKNSYIDFLLSEATTLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQE 341

Query: 1060 QLYQEIESVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIP 1239
             LY+EI  VCGS+K+TEEN+S+LPYL++VFHETLR++SP P++P+RYAHEDT+LGGYHIP
Sbjct: 342  ILYREIHKVCGSNKLTEENLSKLPYLNSVFHETLRKYSPAPMVPVRYAHEDTQLGGYHIP 401

Query: 1240 AGSEIAVNIYGCNMDKHQWAEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAM 1419
            AGS+IA+NIYGCNM+K QW  PE+WKPERFLD KYD MD++KTMAFGGGKRVCAG+ QAM
Sbjct: 402  AGSQIAINIYGCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAM 461

Query: 1420 TISCIAIATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPLHAIITPRQ 1560
             I+C +I  FVQEFEWKL  GE+E+VDT+ LT+ KLHP+ AII  R+
Sbjct: 462  LIACTSIGRFVQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIKARE 508


>ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplastic [Vitis vinifera]
            gi|302142385|emb|CBI19588.3| unnamed protein product
            [Vitis vinifera]
          Length = 508

 Score =  687 bits (1774), Expect = 0.0
 Identities = 327/467 (70%), Positives = 398/467 (85%)
 Frame = +1

Query: 160  PSLPVVPGLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAM 339
            P +P +PGLPLIGNLLQLK+KKPH+TF RWAE YGPI+SI+TGAST++VLN+++VAKEAM
Sbjct: 43   PPVPDIPGLPLIGNLLQLKEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAM 102

Query: 340  VTRFPSISTRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRD 519
            VTRF SISTRKLSNALKILT DKCMVATSDYN+FHK  K +IL   LGA AQKR R HRD
Sbjct: 103  VTRFSSISTRKLSNALKILTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRD 162

Query: 520  TMIKNMVRTLHSYTKDRPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSR 699
            T+I+N+ + LH++ K  PL+    + +FE+E+FGLALKQ LGKD+ES +YVEELGTTLSR
Sbjct: 163  TLIENISKYLHAHVKTSPLEPVVLKKIFESEIFGLALKQALGKDIES-IYVEELGTTLSR 221

Query: 700  EEIFEVLVLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKR 879
            EEIF VLV+DP+ GAI+VDWRDFFPYL W+PNKS++MKI+RM  RR A+MKAL+G++KKR
Sbjct: 222  EEIFAVLVVDPMAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKR 281

Query: 880  IASGEVLNSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKGHQD 1059
            I SGE  N Y+DFL SE  T +E+++ MLIWE IIE SDTTLVT+EWAMYELAK+   Q+
Sbjct: 282  IGSGEEKNCYIDFLLSEATTLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQE 341

Query: 1060 QLYQEIESVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIP 1239
             LY+EI   CGS+KVTEEN+S+LPYL++VFHETLR++SP PI+P+RYAHEDT+LGGYHIP
Sbjct: 342  ILYREIRKFCGSNKVTEENLSKLPYLNSVFHETLRKYSPAPIVPVRYAHEDTQLGGYHIP 401

Query: 1240 AGSEIAVNIYGCNMDKHQWAEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAM 1419
            AGS+IA+NIYGCNM+K QW  PE+WKPERFLD KYD MD++KTMAFGGGKRVCAG+ QAM
Sbjct: 402  AGSQIAINIYGCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAM 461

Query: 1420 TISCIAIATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPLHAIITPRQ 1560
             I+C +I  FVQEFEWKL  GE+E+VDT+ LT+ KLHP+ AII  R+
Sbjct: 462  LIACTSIGRFVQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIKARE 508


>ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus communis]
            gi|223550989|gb|EEF52475.1| ent-kaurene oxidase, putative
            [Ricinus communis]
          Length = 513

 Score =  683 bits (1762), Expect = 0.0
 Identities = 325/474 (68%), Positives = 398/474 (83%)
 Frame = +1

Query: 139  QKSSPRNPSLPVVPGLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTT 318
            +K   R P +P VPG P+IGNLLQLK+KKPH+TF RWAE YGPIYSI+TGAST++VLN+T
Sbjct: 41   KKRHARLPPVPEVPGWPVIGNLLQLKEKKPHKTFMRWAETYGPIYSIRTGASTIIVLNST 100

Query: 319  DVAKEAMVTRFPSISTRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQK 498
            DVAKEAMVTR+ SISTRKLS AL ILT DKCMVATSDY+EFHK  KRY+L+  LGANAQ+
Sbjct: 101  DVAKEAMVTRYSSISTRKLSKALVILTQDKCMVATSDYDEFHKMVKRYLLTNVLGANAQR 160

Query: 499  RFRGHRDTMIKNMVRTLHSYTKDRPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEE 678
            R R HRDT+IKN+    H++ K  P Q  NFR++FE+ELFGL+LK+ LGKD++S +YV+E
Sbjct: 161  RHRCHRDTLIKNISSRFHAHVKTNPEQTVNFRDIFESELFGLSLKEALGKDVQS-IYVDE 219

Query: 679  LGTTLSREEIFEVLVLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKAL 858
            LGTTLSR+EIFEVLVLDP+ GAIDVDWRDFFPYLRWVPNKS +MKI++M  RRKAVM AL
Sbjct: 220  LGTTLSRKEIFEVLVLDPMEGAIDVDWRDFFPYLRWVPNKSWEMKIQQMHFRRKAVMGAL 279

Query: 859  VGQEKKRIASGEVLNSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELA 1038
            + ++KKRIA GE +N Y+DFLSSE  T ++E++ MLIWE I+ETSDTT+V TEWA+YELA
Sbjct: 280  IKEQKKRIALGEEVNCYLDFLSSES-TLTDEQIRMLIWETIVETSDTTMVATEWAIYELA 338

Query: 1039 KNKGHQDQLYQEIESVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTE 1218
            KN   QD LY++I++VCGS+K+TEE++S+LPYL+AVFHET+R++SP P+IP+RY HEDTE
Sbjct: 339  KNPNCQDLLYRQIQNVCGSEKITEEHLSKLPYLNAVFHETIRKYSPAPVIPLRYVHEDTE 398

Query: 1219 LGGYHIPAGSEIAVNIYGCNMDKHQWAEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVC 1398
            LGGY+IPAGSEIA+NIYGCNMDK++W  PE+W PERFL+G YD  D++KTMAFG GKR C
Sbjct: 399  LGGYYIPAGSEIAINIYGCNMDKNRWVNPEEWNPERFLNGNYDSGDLHKTMAFGAGKRAC 458

Query: 1399 AGSQQAMTISCIAIATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPLHAIITPRQ 1560
            AG+ QA  I+  +I   VQEFEW L  GE+EDVD +GLTT KLHPLH  I PR+
Sbjct: 459  AGALQASLIASTSIGRLVQEFEWGLKAGEEEDVDIVGLTTRKLHPLHLSIKPRR 512