BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00001636
         (667 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       249   3e-64
gb|ABC59099.1| cytochrome P450 monooxygenase CYP72A66 [Medicago ...   196   3e-48
ref|XP_003543244.1| PREDICTED: secologanin synthase-like [Glycin...   196   3e-48
ref|NP_188086.1| cytochrome P450, family 72, subfamily A, polype...   196   3e-48
ref|XP_002334834.1| cytochrome P450 [Populus trichocarpa] gi|222...   194   1e-47

>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  249 bits (636), Expect = 3e-64
 Identities = 119/132 (90%), Positives = 125/132 (94%)
 Frame = -1

Query: 667 RLYPPAVSIIRGTYKTMKLGEITLPRGIHLAMPTLLVHHDRELWGEDAEEFNPERFSGGV 488
           RLYPPAV + RGTYKTMKLGEITLP G+HLAMPTLLVHHDRELWGEDAE+FNPERFSGGV
Sbjct: 387 RLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWGEDAEDFNPERFSGGV 446

Query: 487 SNATKNQVSFFPFGWGPRICIGQSFALIESKMAIALILQRFCFELSSTYVHAPSTVITLQ 308
           S ATKNQVSFFPF WGPRICIGQ+FAL+ESKMAIA+ILQRF FELSSTYVHAP TVITLQ
Sbjct: 447 SKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFELSSTYVHAPYTVITLQ 506

Query: 307 PQHGAQLILHKL 272
           PQHGAQLILHKL
Sbjct: 507 PQHGAQLILHKL 518


>gb|ABC59099.1| cytochrome P450 monooxygenase CYP72A66 [Medicago truncatula]
          Length = 395

 Score =  196 bits (499), Expect = 3e-48
 Identities = 87/132 (65%), Positives = 110/132 (83%)
 Frame = -1

Query: 667 RLYPPAVSIIRGTYKTMKLGEITLPRGIHLAMPTLLVHHDRELWGEDAEEFNPERFSGGV 488
           RLYPP + + R   K MKLG +TLP G+ + +P LL+HHD +LWG+DA+ FNPERFSGG+
Sbjct: 262 RLYPPVMGLARNVVKDMKLGNLTLPAGVEVFLPILLIHHDCKLWGDDAKMFNPERFSGGI 321

Query: 487 SNATKNQVSFFPFGWGPRICIGQSFALIESKMAIALILQRFCFELSSTYVHAPSTVITLQ 308
           S AT  +VSFFPFGWGPRICIGQ+F+L+E+KMA+++ILQ F FELSSTY HAPSTVITLQ
Sbjct: 322 SKATNGRVSFFPFGWGPRICIGQNFSLMEAKMALSMILQHFSFELSSTYAHAPSTVITLQ 381

Query: 307 PQHGAQLILHKL 272
           PQ+GA +I+ K+
Sbjct: 382 PQYGAHIIIRKV 393


>ref|XP_003543244.1| PREDICTED: secologanin synthase-like [Glycine max]
          Length = 486

 Score =  196 bits (499), Expect = 3e-48
 Identities = 86/132 (65%), Positives = 110/132 (83%)
 Frame = -1

Query: 667 RLYPPAVSIIRGTYKTMKLGEITLPRGIHLAMPTLLVHHDRELWGEDAEEFNPERFSGGV 488
           RLYPP + + R  ++ MKLG +TLP G+ +++P ++VHHDRELWG+DA+EFNPERFS GV
Sbjct: 353 RLYPPGIGLTRSVHRDMKLGNLTLPAGVQVSLPIIMVHHDRELWGDDAKEFNPERFSEGV 412

Query: 487 SNATKNQVSFFPFGWGPRICIGQSFALIESKMAIALILQRFCFELSSTYVHAPSTVITLQ 308
           S AT  +VSFFPFGWGPRICIGQ+F+L+E+KMA+++ILQ F FELS  Y HAP TVITLQ
Sbjct: 413 SKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQHFSFELSPAYTHAPFTVITLQ 472

Query: 307 PQHGAQLILHKL 272
           PQ+GA +IL K+
Sbjct: 473 PQYGAHVILRKV 484


>ref|NP_188086.1| cytochrome P450, family 72, subfamily A, polypeptide 14
           [Arabidopsis thaliana]
           gi|13605897|gb|AAK32934.1|AF367347_1 AT3g14680/MIE1_18
           [Arabidopsis thaliana] gi|9294390|dbj|BAB02400.1|
           cytochrome P450 [Arabidopsis thaliana]
           gi|24111277|gb|AAN46762.1| At3g14680/MIE1_18
           [Arabidopsis thaliana] gi|332642034|gb|AEE75555.1|
           cytochrome P450, family 72, subfamily A, polypeptide 14
           [Arabidopsis thaliana]
          Length = 512

 Score =  196 bits (498), Expect = 3e-48
 Identities = 90/132 (68%), Positives = 107/132 (81%)
 Frame = -1

Query: 667 RLYPPAVSIIRGTYKTMKLGEITLPRGIHLAMPTLLVHHDRELWGEDAEEFNPERFSGGV 488
           RLYPP V + R  +K MKLG++TLP G+ +++P LLVH D ELWG DA EF PERF  G+
Sbjct: 381 RLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPERFKDGL 440

Query: 487 SNATKNQVSFFPFGWGPRICIGQSFALIESKMAIALILQRFCFELSSTYVHAPSTVITLQ 308
           S ATKNQVSFFPF WGPRICIGQ+F L+E+KMA++LILQRF FELS +YVHAP T+ITL 
Sbjct: 441 SKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYTIITLY 500

Query: 307 PQHGAQLILHKL 272
           PQ GA L+LHKL
Sbjct: 501 PQFGAHLMLHKL 512


>ref|XP_002334834.1| cytochrome P450 [Populus trichocarpa] gi|222875161|gb|EEF12292.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 353

 Score =  194 bits (494), Expect = 1e-47
 Identities = 85/132 (64%), Positives = 111/132 (84%)
 Frame = -1

Query: 667 RLYPPAVSIIRGTYKTMKLGEITLPRGIHLAMPTLLVHHDRELWGEDAEEFNPERFSGGV 488
           RLYPP +++ R  ++ +KLG + LP G+ +++PT+L+H D ELWG+DA EF PERF+ GV
Sbjct: 222 RLYPPVITLNRDVHEEIKLGNLLLPAGVQVSLPTILLHQDHELWGDDASEFKPERFAEGV 281

Query: 487 SNATKNQVSFFPFGWGPRICIGQSFALIESKMAIALILQRFCFELSSTYVHAPSTVITLQ 308
           S ATK+QVSF PFGWGPRIC+GQ+FALIE+KMA+A++LQR+ FELS +Y+HAP TVITLQ
Sbjct: 282 SKATKSQVSFLPFGWGPRICVGQNFALIEAKMALAMVLQRYSFELSPSYIHAPRTVITLQ 341

Query: 307 PQHGAQLILHKL 272
           PQHGA +IL KL
Sbjct: 342 PQHGAPMILRKL 353