BLASTX nr result
ID: Coptis21_contig00001443
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00001443
(1394 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bon... 499 e-139
ref|XP_002873802.1| hypothetical protein ARALYDRAFT_909686 [Arab... 497 e-138
ref|NP_197201.1| 2-alkenal reductase [Arabidopsis thaliana] gi|2... 495 e-137
ref|XP_002873801.1| AT-AER [Arabidopsis lyrata subsp. lyrata] gi... 495 e-137
pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Red... 494 e-137
>ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bond reductase P2 [Vitis
vinifera] gi|147792339|emb|CAN61471.1| hypothetical
protein VITISV_043825 [Vitis vinifera]
Length = 345
Score = 499 bits (1285), Expect = e-139
Identities = 244/346 (70%), Positives = 291/346 (84%)
Frame = +2
Query: 41 QSMENKQIVLKDHVVGYPRETDMELRRSTLEPKVVEGGGAVLLKNLYLSCDPYMRGRMSK 220
+++ NKQ++ +D+V G+P+E+DM + ST+ KV EG AVL+KNLYLSCDPYMR RM
Sbjct: 5 EAVSNKQVIFRDYVSGFPKESDMYVTTSTISLKVPEGSEAVLVKNLYLSCDPYMRSRM-- 62
Query: 221 PRPDTQSYSNSLVPGSPINGYGVSKVVESGHKDYKEGDLVWGITGWEQFTLLPNPNPAVL 400
R SY PGSPI GYGV+KV++S H D++ GDLVWGITGWE+++L+ L
Sbjct: 63 -RNIQGSYVEPFKPGSPITGYGVAKVLDSRHPDFRTGDLVWGITGWEEYSLITTDER--L 119
Query: 401 TKIKYTDVPLSYYTGILGMPGLTAYVGFHEICSPKKGETVYVSAASGAVGQLVGQFAKLM 580
KI++TDVPLSYYTGILGM G+TAY GF+EICSPKKGE V++SAASGAVGQLVGQFAKL+
Sbjct: 120 FKIQHTDVPLSYYTGILGMAGMTAYSGFYEICSPKKGEYVFISAASGAVGQLVGQFAKLL 179
Query: 581 GCYVVGSAGTSEKVELLKNKFGFDDAFNYKEEQDLTAALKRCFPEGIDIYFENVGGRMLD 760
GCYVVGSAGT EKV+LLKNKFGFD+AFNYKEEQDL A LKR FPEGIDIYFENVGG+MLD
Sbjct: 180 GCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLEACLKRYFPEGIDIYFENVGGKMLD 239
Query: 761 AVLLNMNVHGRIAVCGLISQYNLTEHEGVRNLFCIITKRIRMQGFIEPDYKHMYPEFLEL 940
AVL+NM +HGRIAVCG+ISQYNL E EGVRNLF I+TKRIRM+GF+ DY H+YP+FL+L
Sbjct: 240 AVLVNMRLHGRIAVCGMISQYNLEEPEGVRNLFTIVTKRIRMEGFLVFDYYHLYPKFLDL 299
Query: 941 VIQYIREGKIVYIEDTAEGLEEAPSALIGLFSGRNVGKQVVRIADE 1078
++ YIREGKIVY+ED AEGLE AP+ALIGL+SGRNVGKQVV +A E
Sbjct: 300 IMPYIREGKIVYVEDIAEGLESAPTALIGLYSGRNVGKQVVVVARE 345
>ref|XP_002873802.1| hypothetical protein ARALYDRAFT_909686 [Arabidopsis lyrata subsp.
lyrata] gi|297319639|gb|EFH50061.1| hypothetical protein
ARALYDRAFT_909686 [Arabidopsis lyrata subsp. lyrata]
Length = 346
Score = 497 bits (1279), Expect = e-138
Identities = 239/347 (68%), Positives = 288/347 (82%)
Frame = +2
Query: 38 TQSMENKQIVLKDHVVGYPRETDMELRRSTLEPKVVEGGGAVLLKNLYLSCDPYMRGRMS 217
T + NKQ+ LKD+V G+P+E+D + +T+E KV EG +VL+KNLYLSCDPYMR RMS
Sbjct: 2 TTTATNKQVTLKDYVSGFPKESDFDFTTTTVELKVPEGSNSVLVKNLYLSCDPYMRTRMS 61
Query: 218 KPRPDTQSYSNSLVPGSPINGYGVSKVVESGHKDYKEGDLVWGITGWEQFTLLPNPNPAV 397
KP+P T + + + PG PI+GYGVS+V+ESGH DY++GDL+WGI GWE+++++ P P V
Sbjct: 62 KPKPST-ALAQAYTPGQPISGYGVSRVIESGHPDYQKGDLLWGIVGWEEYSVI-TPTPQV 119
Query: 398 LTKIKYTDVPLSYYTGILGMPGLTAYVGFHEICSPKKGETVYVSAASGAVGQLVGQFAKL 577
KI++TDVPLSYYTG+LGMPG+TAY GF+E+CSPKKGETVYVSAASGAVGQLVGQ AK+
Sbjct: 120 HFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQLAKM 179
Query: 578 MGCYVVGSAGTSEKVELLKNKFGFDDAFNYKEEQDLTAALKRCFPEGIDIYFENVGGRML 757
MGCYVVGSAG+ EKV+LLK KFGFDDAFNYKEE DL+AALKRCFP+GIDIYFENVGG+ML
Sbjct: 180 MGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEEPDLSAALKRCFPKGIDIYFENVGGKML 239
Query: 758 DAVLLNMNVHGRIAVCGLISQYNLTEHEGVRNLFCIITKRIRMQGFIEPDYKHMYPEFLE 937
DAVLLNMN HGRIAVCG+ISQYNL EGV NL II KRIR+QGF DY Y +FLE
Sbjct: 240 DAVLLNMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFAVFDYYDKYSKFLE 299
Query: 938 LVIQYIREGKIVYIEDTAEGLEEAPSALIGLFSGRNVGKQVVRIADE 1078
V+ I+EGKI Y+ED A+GLE+ P AL+GLF G+NVGKQVV +A E
Sbjct: 300 FVLPCIKEGKITYVEDVADGLEKGPEALVGLFHGKNVGKQVVVVARE 346
>ref|NP_197201.1| 2-alkenal reductase [Arabidopsis thaliana]
gi|21431800|sp|Q39173.2|P2_ARATH RecName:
Full=NADP-dependent alkenal double bond reductase P2
gi|9755700|emb|CAC01712.1| quinone oxidoreductase-like
protein [Arabidopsis thaliana] gi|17529038|gb|AAL38729.1|
putative quinone oxidoreductase [Arabidopsis thaliana]
gi|20259087|gb|AAM14259.1| putative quinone
oxidoreductase [Arabidopsis thaliana]
gi|332004984|gb|AED92367.1| 2-alkenal reductase
[Arabidopsis thaliana]
Length = 343
Score = 495 bits (1275), Expect = e-137
Identities = 237/342 (69%), Positives = 286/342 (83%)
Frame = +2
Query: 53 NKQIVLKDHVVGYPRETDMELRRSTLEPKVVEGGGAVLLKNLYLSCDPYMRGRMSKPRPD 232
NKQ++ KDHV G+P+E+D +T+E +V EG +VL+KNLYLSCDPYMR RM KP P
Sbjct: 4 NKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKPDPS 63
Query: 233 TQSYSNSLVPGSPINGYGVSKVVESGHKDYKEGDLVWGITGWEQFTLLPNPNPAVLTKIK 412
+ + + + PG PI GYGVS+V+ESGH DYK+GDL+WGI GWE+++++ P + KI+
Sbjct: 64 S-ALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVI-TPMAHMHFKIQ 121
Query: 413 YTDVPLSYYTGILGMPGLTAYVGFHEICSPKKGETVYVSAASGAVGQLVGQFAKLMGCYV 592
+TDVPLSYYTG+LGMPG+TAY GF+E+CSPKKGETVYVSAASGAVGQLVGQFAK+MGCYV
Sbjct: 122 HTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCYV 181
Query: 593 VGSAGTSEKVELLKNKFGFDDAFNYKEEQDLTAALKRCFPEGIDIYFENVGGRMLDAVLL 772
VGSAG+ EKV+LLK KFGFDDAFNYKEE DL+AALKRCFP+GID+YFENVGG+MLDAVLL
Sbjct: 182 VGSAGSKEKVDLLKTKFGFDDAFNYKEESDLSAALKRCFPKGIDMYFENVGGKMLDAVLL 241
Query: 773 NMNVHGRIAVCGLISQYNLTEHEGVRNLFCIITKRIRMQGFIEPDYKHMYPEFLELVIQY 952
NMN HGRIAVCG+ISQYNL EGV NL II KRIR+QGF+ D+ YP+FLELV+
Sbjct: 242 NMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVADFYDKYPKFLELVLPR 301
Query: 953 IREGKIVYIEDTAEGLEEAPSALIGLFSGRNVGKQVVRIADE 1078
I+EGKI Y+ED A+GLE+AP AL+GLF G+NVGKQVV IA E
Sbjct: 302 IKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIARE 343
>ref|XP_002873801.1| AT-AER [Arabidopsis lyrata subsp. lyrata] gi|297319638|gb|EFH50060.1|
AT-AER [Arabidopsis lyrata subsp. lyrata]
Length = 345
Score = 495 bits (1274), Expect = e-137
Identities = 237/342 (69%), Positives = 284/342 (83%)
Frame = +2
Query: 53 NKQIVLKDHVVGYPRETDMELRRSTLEPKVVEGGGAVLLKNLYLSCDPYMRGRMSKPRPD 232
NKQ++LKD+V G+P+E+D + +T+E KV EG VL+KNLYLSCDPYMR RM KP P
Sbjct: 5 NKQVILKDYVNGFPKESDFDFTTTTVELKVPEGSNLVLVKNLYLSCDPYMRIRMGKPDPS 64
Query: 233 TQSYSNSLVPGSPINGYGVSKVVESGHKDYKEGDLVWGITGWEQFTLLPNPNPAVLTKIK 412
T + + + PG PI GY VS+VVESGH DYK+GDL+WGI WE+++++ P P V KI+
Sbjct: 65 TAALAQAYTPGQPIQGYVVSRVVESGHPDYKKGDLLWGIVAWEEYSVI-TPTPHVHFKIQ 123
Query: 413 YTDVPLSYYTGILGMPGLTAYVGFHEICSPKKGETVYVSAASGAVGQLVGQFAKLMGCYV 592
+TDVPLSYYTG+LGMPG+TAY GF+E+CSPKKGETVYVSAASGAVGQLVGQFAK++GCYV
Sbjct: 124 HTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMLGCYV 183
Query: 593 VGSAGTSEKVELLKNKFGFDDAFNYKEEQDLTAALKRCFPEGIDIYFENVGGRMLDAVLL 772
VGSAG+ EKV+LLK KFGFDDAFNYKEE DLTAALKRCFP GIDIYFENVGG+ML+AVL+
Sbjct: 184 VGSAGSKEKVDLLKTKFGFDDAFNYKEEPDLTAALKRCFPNGIDIYFENVGGKMLNAVLV 243
Query: 773 NMNVHGRIAVCGLISQYNLTEHEGVRNLFCIITKRIRMQGFIEPDYKHMYPEFLELVIQY 952
NMN HGRIAVCG+ISQYNL EGV NL II KRIR+QGF+ D+ Y +FLE V+ +
Sbjct: 244 NMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVFDFYDKYSKFLEFVLPH 303
Query: 953 IREGKIVYIEDTAEGLEEAPSALIGLFSGRNVGKQVVRIADE 1078
IREGKI Y+ED A+GLE+AP AL+GLF G+NVGKQVV +A E
Sbjct: 304 IREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVARE 345
>pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Reductase From
Nicotiana Tabacum gi|444302247|pdb|4HFJ|B Chain B, X-ray
Crystal Structure Of A Double Bond Reductase From
Nicotiana Tabacum gi|444302248|pdb|4HFM|A Chain A, X-ray
Crystal Structure Of A Nadp(h)-bound Double Bond
Reductase From Nicotiana Tabacum gi|444302249|pdb|4HFM|B
Chain B, X-ray Crystal Structure Of A Nadp(h)-bound
Double Bond Reductase From Nicotiana Tabacum
gi|444302250|pdb|4HFN|A Chain A, X-ray Crystal Structure
Of A Ternary Complex Of Double Bond Reductase From
Nicotiana Tabacum gi|444302251|pdb|4HFN|B Chain B, X-ray
Crystal Structure Of A Ternary Complex Of Double Bond
Reductase From Nicotiana Tabacum
Length = 351
Score = 494 bits (1273), Expect = e-137
Identities = 236/353 (66%), Positives = 293/353 (83%)
Frame = +2
Query: 41 QSMENKQIVLKDHVVGYPRETDMELRRSTLEPKVVEGGGAVLLKNLYLSCDPYMRGRMSK 220
+ + NKQ++LK++V GYP+E+DME++ T++ KV EG V++KNLYLSCDPYMR RM K
Sbjct: 3 EEVSNKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRMRK 62
Query: 221 PRPDTQSYSNSLVPGSPINGYGVSKVVESGHKDYKEGDLVWGITGWEQFTLLPNPNPAVL 400
SY S PGSPI GYGV+KV+ESG +++GDLVWG+TGWE+++++ L
Sbjct: 63 IEG---SYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQ--TL 117
Query: 401 TKIKYTDVPLSYYTGILGMPGLTAYVGFHEICSPKKGETVYVSAASGAVGQLVGQFAKLM 580
KI DVPLSYYTGILGMPG+TAY GFHE+CSPKKGETV+VSAASGAVGQLVGQFAK++
Sbjct: 118 FKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKML 177
Query: 581 GCYVVGSAGTSEKVELLKNKFGFDDAFNYKEEQDLTAALKRCFPEGIDIYFENVGGRMLD 760
GCYVVGSAG+ EKV+LLK+KFGFD+AFNYKEEQDL+AALKR FP+GIDIYFENVGG+MLD
Sbjct: 178 GCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLD 237
Query: 761 AVLLNMNVHGRIAVCGLISQYNLTEHEGVRNLFCIITKRIRMQGFIEPDYKHMYPEFLEL 940
AVL+NM ++GRIAVCG+ISQYNL + EGV NLFC+ITKRIRM+GF+ DY H+YP++LE+
Sbjct: 238 AVLVNMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEM 297
Query: 941 VIQYIREGKIVYIEDTAEGLEEAPSALIGLFSGRNVGKQVVRIADE*LRWVHH 1099
VI I+ GK+VY+ED A GLE AP+AL+GLFSGRN+GKQVV ++ E HH
Sbjct: 298 VIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVSRELEHHHHH 350