BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00001380
         (485 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       317   8e-85
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   223   1e-56
gb|AGC29950.1| CYP81B56, partial [Sinopodophyllum hexandrum]          194   5e-48
emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]   191   4e-47
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   191   5e-47

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  317 bits (811), Expect = 8e-85
 Identities = 159/161 (98%), Positives = 161/161 (100%)
 Frame = +1

Query: 1   MIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGVKGVEKKVWNLK 180
           MIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIG+KG+EKKVWNLK
Sbjct: 182 MIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLK 241

Query: 181 EKRDKFLQELIEEHRISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLA 360
           EKRDKFLQELIEEHRISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLA
Sbjct: 242 EKRDKFLQELIEEHRISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLA 301

Query: 361 AGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI 483
           AGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI
Sbjct: 302 AGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI 342


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  223 bits (568), Expect = 1e-56
 Identities = 113/163 (69%), Positives = 133/163 (81%), Gaps = 2/163 (1%)
 Frame = +1

Query: 1   MIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGVKGVEKKVWNLK 180
           MIAGKRYYG  V +LEQA++F+EIV+ T  +SGASN+GDFLP+L WIG KG+EK++  LK
Sbjct: 183 MIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLK 242

Query: 181 EKRDKFLQELIEEHRISR--SGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVL 354
            KRD+FLQELI+E R  R  S    ++EK  TLIDVLLSLQETEPEYYTDEMI G+IW+L
Sbjct: 243 NKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWIL 302

Query: 355 LAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI 483
            AAGTDTSA TMEWAMSLLLNNP +IKKAQ+EID  +E GR I
Sbjct: 303 FAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPI 345


>gb|AGC29950.1| CYP81B56, partial [Sinopodophyllum hexandrum]
          Length = 344

 Score =  194 bits (494), Expect = 5e-48
 Identities = 100/162 (61%), Positives = 127/162 (78%), Gaps = 2/162 (1%)
 Frame = +1

Query: 4   IAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGVKGVEKKVWNLKE 183
           I+GK YYG   E  E+A RF+EIV+ T A+ GASN+ DFLP+L W+G +G EKK   LK 
Sbjct: 23  ISGKSYYGE--EHSEEAMRFEEIVEETFAMGGASNVVDFLPVLRWVGSRGTEKKFAELKR 80

Query: 184 KRDKFLQELIEEHR-ISRSGGMQTKEK-NATLIDVLLSLQETEPEYYTDEMILGIIWVLL 357
           KRD+FL+EL+E+ R + R+GG    +    TLIDVLL+LQ  E +YYTD++I+GIIW++L
Sbjct: 81  KRDEFLKELLEQQRRLKRNGGGGDGDLVKMTLIDVLLTLQVNESDYYTDDIIIGIIWIML 140

Query: 358 AAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI 483
           +AGTDTSA TMEWAMSLLLNNP+VIKKAQ E+DNN+E GRLI
Sbjct: 141 SAGTDTSAGTMEWAMSLLLNNPEVIKKAQLEMDNNIEPGRLI 182


>emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]
          Length = 1001

 Score =  191 bits (486), Expect = 4e-47
 Identities = 97/161 (60%), Positives = 127/161 (78%)
 Frame = +1

Query: 1    MIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGVKGVEKKVWNLK 180
            MI+GKRYYG  V ELE+ ++F+EIV  T  +SGA+NI DF+P   WIG+ G+EKK+  L+
Sbjct: 683  MISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVDFVPFSKWIGLNGIEKKLVILQ 742

Query: 181  EKRDKFLQELIEEHRISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLA 360
             KRD F+Q LIEEHR  RS     + ++ T++DVLLSLQETEPE YTD++I G++ V+L+
Sbjct: 743  GKRDGFMQNLIEEHRRMRS---PCEXRSKTMLDVLLSLQETEPECYTDDIIRGMMQVMLS 799

Query: 361  AGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI 483
            AGTDTSA TMEWAMSLLLNNP+ ++KAQAEID++L + RLI
Sbjct: 800  AGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSRLI 840


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  191 bits (485), Expect = 5e-47
 Identities = 97/161 (60%), Positives = 127/161 (78%)
 Frame = +1

Query: 1   MIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIGVKGVEKKVWNLK 180
           MI+GKRYYG  V ELE+ ++F+EIV  T  +SGA+NI DF+P   WIG+ G+EKK+  L+
Sbjct: 185 MISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVDFVPFSKWIGLNGIEKKLVILQ 244

Query: 181 EKRDKFLQELIEEHRISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLA 360
            KRD F+Q LIEEHR  RS     + ++ T++DVLLSLQETEPE YTD++I G++ V+L+
Sbjct: 245 GKRDGFMQNLIEEHRRMRS---PCEGRSKTMLDVLLSLQETEPECYTDDIIRGMMQVMLS 301

Query: 361 AGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLI 483
           AGTDTSA TMEWAMSLLLNNP+ ++KAQAEID++L + RLI
Sbjct: 302 AGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSRLI 342