BLASTX nr result
ID: Coptis21_contig00001143
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00001143
(1399 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002514982.1| 3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransf... 325 2e-86
ref|XP_002271612.1| PREDICTED: vinorine synthase-like [Vitis vin... 325 2e-86
gb|ADD71922.1| BAHD-type acyltransferase [Actaea racemosa] 323 9e-86
ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|2... 311 3e-82
ref|XP_002514981.1| Salutaridinol 7-O-acetyltransferase, putativ... 308 3e-81
>ref|XP_002514982.1| 3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase, putative [Ricinus
communis] gi|223546033|gb|EEF47536.1|
3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase,
putative [Ricinus communis]
Length = 441
Score = 325 bits (833), Expect = 2e-86
Identities = 189/429 (44%), Positives = 261/429 (60%), Gaps = 11/429 (2%)
Frame = +2
Query: 11 MTLDLKVLSQETIKPSSPTPNHLRNFKLSLADQHAPPAAISLILIYPTNN-------NDR 169
M +++K++ ++ IKPSSPTPNHLR FKLSL DQ +P A S++L+Y N +
Sbjct: 1 MEINVKIIKKDIIKPSSPTPNHLRIFKLSLLDQLSPAAYGSMLLVYSINKPSTAIDKSQL 60
Query: 170 LKRSLSEILTHFYPLAGTIDNNQFIDCNDEGVNFYEARVT---GRQLSQILNHPIPEELD 340
LKRSLSE LT FYPLAG I +N ++CND+G + EA+V + L + +H + L
Sbjct: 61 LKRSLSETLTRFYPLAGKIKDNSAVECNDDGAVYLEAQVDCLLSKLLEKPDDHQVIRNLI 120
Query: 341 QLLHRNDFYRVGPKPLLSIQANIFDCGGMALGVCISHKAADASSLGLFLDHWAAIARG-D 517
H LL +QA F CGG+A+GVCISHK ADA ++ F+ WAA A G D
Sbjct: 121 PAEHIQSLSETQAGCLLLVQATFFACGGLAIGVCISHKLADARTVCTFIQGWAAAALGTD 180
Query: 518 NEVPQPKLVQTSLFPPKDVPSYVPFGASPIQKVVNKFFVFNAGNIAALKAKASHKSYVEH 697
+E +PK +S+FPP+++PS + + +K V K +VF+A IA LK KA+ +S V H
Sbjct: 181 HEAVRPKFNVSSIFPPQNLPSELAVKLNE-EKCVTKRYVFHASGIATLKDKAASES-VRH 238
Query: 698 PTRVEAVSALIWRCALNASRTRPGSVKSESAMILAMNLRGKMVPALSNLSFGNFMIGVTA 877
PTRVEAV+ALIW+CA+ ASR+ K S + ++N+R K+VP LS S GN M
Sbjct: 239 PTRVEAVTALIWKCAMKASRSNSKQPKM-SVLAQSVNIRKKLVPPLSEYSIGNLMGHFAL 297
Query: 878 TTIESAPELHCLVGELREEKMKVDDDYVRKLQVDDGLALLWNSVKDLNVRHIKGELELYT 1057
E+ EL LV ++R+ + ++YV+KLQ + L ++ ++K++ G E Y
Sbjct: 298 RATENEVELGTLVTKMRKGIQEFGENYVKKLQEGNALKAVFEALKEMGSLLHGGSTEFYI 357
Query: 1058 TSSWCGFPFYEADFGWGKPMWISSVNLVTPNIIMLMDSKCGIGVEASVTLDEEVMARFEC 1237
TSS C FPFY DFGWGKP W+ NII L+D++ G G+EA VTL EE MA FE
Sbjct: 358 TSSLCRFPFYGIDFGWGKPAWVVPPRDPIKNIISLIDARDGNGIEAWVTLKEEDMAIFER 417
Query: 1238 DPELIAFTS 1264
D EL+A S
Sbjct: 418 DQELLAVAS 426
>ref|XP_002271612.1| PREDICTED: vinorine synthase-like [Vitis vinifera]
Length = 433
Score = 325 bits (832), Expect = 2e-86
Identities = 185/439 (42%), Positives = 271/439 (61%), Gaps = 18/439 (4%)
Frame = +2
Query: 11 MTLDLKVLSQETIKPSSPTPNHLRNFKLSLADQHAPPAAISLILIYPTNNND-------- 166
M + + +LS + IKPSSPTPNHLR+FK+SL DQ APP + +IL++ ++ D
Sbjct: 1 MEMKIDILSTQPIKPSSPTPNHLRSFKISLLDQLAPPFYVPVILLFSADDFDCEAVDHVT 60
Query: 167 ---RLKRSLSEILTHFYPLAGTIDNNQFIDCNDEGVNFYEARVTGRQLSQILNHPIPEEL 337
LKRSLS+ L+ FYPLAG + N +DC+D+G F EAR +LS+IL P + L
Sbjct: 61 ICDLLKRSLSQTLSRFYPLAGKLKGNDSVDCSDDGAVFMEARANV-ELSEILRDPEIDLL 119
Query: 338 DQLLHRNDFYRVGP----KPLLSIQANIFDCGGMALGVCISHKAADASSLGLFLDHWAAI 505
+LL Y VG + + +IQA IF+CGG+ +GVC+SHK AD ++L FL W+A
Sbjct: 120 QKLLPCEP-YSVGSESSDRAITAIQATIFECGGIGIGVCMSHKVADGATLATFLTAWSAT 178
Query: 506 ARGDNEVPQPKLVQTSLFPPKDVPSYVPFGASPIQKVVNKFFVFNAGNIAALKAKASHKS 685
A G ++ P L SLFPP+D+ + + G K + + F+F+A ++A L++KAS+
Sbjct: 179 AMGTDDGITPFLDSASLFPPRDINTVLSSGVISHGKTLTRRFLFDAASLARLQSKASNS- 237
Query: 686 YVEHPTRVEAVSALIWRCALNASRTRPGSVKSESAMILAMNLRGKMVPALSNLSFGNF-- 859
TRVEAV++LIW+ A++ +R + G S + +NLRGK P LS+ S GN
Sbjct: 238 -----TRVEAVTSLIWKSAMDVAREKSGKDTISSIVTHVVNLRGKTEPPLSDRSLGNLWQ 292
Query: 860 MIGVTATTIESAPELHCLVGELREEKMKVDDDYVRKLQVDDGLALLWNSVKDLNVRHI-K 1036
T T E EL LVG LR KVD +YV+++Q ++GL+ ++K++ + K
Sbjct: 293 QAVATVTEQEGKVELDDLVGRLRRAIKKVDKEYVKEIQGEEGLSKACGAMKEVQKMIMSK 352
Query: 1037 GELELYTTSSWCGFPFYEADFGWGKPMWISSVNLVTPNIIMLMDSKCGIGVEASVTLDEE 1216
GE+ELY SSW FPFYE DFG+G+P+W+ ++ N+I+LMD+K G G+EA VT+ EE
Sbjct: 353 GEMELYRFSSWSRFPFYETDFGFGRPIWVCTITAPIKNVIILMDTKSGGGIEAWVTMVEE 412
Query: 1217 VMARFECDPELIAFTSTTK 1273
M +F+ EL+ F S+T+
Sbjct: 413 DMTKFQRHYELLEFVSSTQ 431
>gb|ADD71922.1| BAHD-type acyltransferase [Actaea racemosa]
Length = 424
Score = 323 bits (827), Expect = 9e-86
Identities = 196/434 (45%), Positives = 264/434 (60%), Gaps = 19/434 (4%)
Frame = +2
Query: 17 LDLKVLSQETIKPSSPTPNHLRNFKLSLADQHAPPAAISLILIYP----------TNNND 166
+++ V S+E IKPSSPTPNHL+ SL DQ++ +S++L Y TN +D
Sbjct: 1 MEVVVSSREIIKPSSPTPNHLKKLNFSLNDQYSTHY-VSVLLFYSAQGDVDRFKQTNTSD 59
Query: 167 RLKRSLSEILTHFYPLAGTIDNNQFIDCNDEGVNFYEARVTGRQLSQILNHPIPEELDQL 346
RLK+SLSEILT FYPLAG I NN+ IDCND+G+ + EARV LSQ+L P +EL+QL
Sbjct: 60 RLKKSLSEILTQFYPLAGRIINNECIDCNDDGLEYLEARVPC-PLSQLLGCPKADELNQL 118
Query: 347 LHRNDFYRVGPKPLLSIQANIFDCGGMALGVCISHKAADASSLGLFLDHWAAIARGDNEV 526
+ + + L ++Q ++FDCGG+A+GV ISH A DASSL F++ WAA A+G NE+
Sbjct: 119 IPFS-------QKLSAVQVSLFDCGGIAIGVTISHTAGDASSLTAFINSWAATAKGANEI 171
Query: 527 PQPKLVQTSLFPPKDVPSYVPFGASPIQ-----KVVNKFFVFNAGNIAALKAKASHKSYV 691
PK LFPP+DVP+ V FG + + V K F+F++ +AALK+ + V
Sbjct: 172 VPPKFGFDYLFPPRDVPT-VSFGGGAVDYTQLPRFVGKRFIFDSSKLAALKSACAD---V 227
Query: 692 EHPTRVEAVSALIWRCALNASRTRPGSVKSESAMILAMNLRGKMVPALSNLSFGNFMIGV 871
E PTRVE V+A I++C LN R+R S + + +NLRG+M P L SFGN I +
Sbjct: 228 ERPTRVEVVTAFIYKCFLNTIRSRS---SKPSVLSMPINLRGRMNPPLPPHSFGNLAIRL 284
Query: 872 TAT--TIESAPELHCLVGELREEKMKVDDDYVRKLQVDDGLALL--WNSVKDLNVRHIKG 1039
T+ E PEL+CLV +LRE KV+ +V KLQ D+ L W K + G
Sbjct: 285 TSQPWPAEKEPELNCLVKQLRETIRKVNGGFVEKLQADNAELLFEHWKDWKKGIELSLTG 344
Query: 1040 ELELYTTSSWCGFPFYEADFGWGKPMWISSVNLVTPNIIMLMDSKCGIGVEASVTLDEEV 1219
+L + C FPFYEADFGWGKP W + VNL + L+D+K G GVEA VTL E
Sbjct: 345 DLNVLMVLICCRFPFYEADFGWGKPAWATRVNLYPS--VSLVDTKDGEGVEAWVTLAEGD 402
Query: 1220 MARFECDPELIAFT 1261
M RF +P+L+ F+
Sbjct: 403 MTRFCSEPDLLDFS 416
>ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|222864679|gb|EEF01810.1|
predicted protein [Populus trichocarpa]
Length = 430
Score = 311 bits (797), Expect = 3e-82
Identities = 182/440 (41%), Positives = 270/440 (61%), Gaps = 19/440 (4%)
Frame = +2
Query: 11 MTLDLKVLSQETIKPSSPTPNHLRNFKLSLADQHAPPAAISLILIYPTNNN--------- 163
M ++++V+S E IKPSSPTP+HL +++LS DQ +PP L+L YP + +
Sbjct: 1 MKIEIEVISNEIIKPSSPTPDHLCHYQLSFLDQISPPTYNPLLLFYPADGDVKINNIEKP 60
Query: 164 DRLKRSLSEILTHFYPLAGTIDNNQFIDCNDEGVNFYEARVTGRQLSQILNHPIPEELDQ 343
++LK+SLSE+L +YPLAG I +N F++CNDEG+ F++A V R L Q++ +P P EL++
Sbjct: 61 NQLKQSLSEVLNLYYPLAGRIKDNLFVECNDEGIPFFQAEVKCR-LPQVVENPEPSELNK 119
Query: 344 LLHRNDFYRVGPKPL-LSIQANIFDCGGMALGVCISHKAADASSLGLFLDHWAAIARGD- 517
L+ F + L L IQ NIF+CGG+ +G+CISHK DASSL F+ +WAA ARG+
Sbjct: 120 LI---PFALDDAEELPLGIQYNIFECGGIVIGLCISHKVGDASSLFTFIKYWAATARGEA 176
Query: 518 NEVPQPKLVQTSLFPPKDVPSYVPFGASPIQKVVNKFFVFNAGNIAALKAKASHKS---- 685
+ + +P+ + +LFPP ++ + P + VV K FVF + +I LK K S S
Sbjct: 177 DHISRPEFISATLFPPINISGFKPATGITKEDVVTKRFVFRSSSIELLKEKCSPASGSLE 236
Query: 686 YVEHPTRVEAVSALIWRCALNASR--TRPGSVKSESAMILAMNLRGKMVPALSNLSFGNF 859
P+RVEA+S IW+ AS+ +RP + S M+ A+NLR +M P L SFGN+
Sbjct: 237 NQRPPSRVEALSVFIWQRFTAASKVESRPERIYS---MVHAVNLRSRMEPPLPEYSFGNY 293
Query: 860 M-IGVTATTIESAPELHCLVGELREEKMKVDDDYVRKLQV-DDGLALLWNSVKDLNVRHI 1033
I T +I++ E + L ++R+ KVD +YV+KLQ ++ L + K+ R +
Sbjct: 294 CRIAFTIPSIDTGEENYNLASQIRDSIGKVDKEYVKKLQKGNEHLGFM----KEQAARFL 349
Query: 1034 KGELELYTTSSWCGFPFYEADFGWGKPMWISSVNLVTPNIIMLMDSKCGIGVEASVTLDE 1213
+GE+ +S C FP YEADFGW KP+W+ S +L N+++ MD+ G G+EA V L E
Sbjct: 350 RGEVVPLNFTSLCRFPLYEADFGWAKPIWVGSPSLTFKNLVVFMDTASGDGIEALVHLKE 409
Query: 1214 EVMARFECDPELIAFTSTTK 1273
E MA+FE D EL+ + TK
Sbjct: 410 EDMAKFEEDEELLQYIVPTK 429
>ref|XP_002514981.1| Salutaridinol 7-O-acetyltransferase, putative [Ricinus communis]
gi|223546032|gb|EEF47535.1| Salutaridinol
7-O-acetyltransferase, putative [Ricinus communis]
Length = 430
Score = 308 bits (788), Expect = 3e-81
Identities = 180/438 (41%), Positives = 265/438 (60%), Gaps = 20/438 (4%)
Frame = +2
Query: 11 MTLDLKVLSQETIKPSSPTPNHLRNFKLSLADQHAPPAAISLILIYPTN-----NN---- 163
M L+++V+S+E IKPSSPTP+HLR++KLS DQ +PP L+L YPT+ NN
Sbjct: 1 MKLEIEVISEEIIKPSSPTPDHLRHYKLSFLDQISPPVYNPLLLFYPTDGRVKSNNAQKY 60
Query: 164 DRLKRSLSEILTHFYPLAGTIDNNQFIDCNDEGVNFYEARVTGRQLSQILNHPIPEELDQ 343
D+LK+SLS++LT++YPLAG I +N +DCNDEGV F +A+ +LS +N+P+P E +
Sbjct: 61 DQLKQSLSQVLTYYYPLAGRIKDNNLVDCNDEGVPFLQAQAIACRLSDAVNNPMPTEFRK 120
Query: 344 LLHRNDFYRVGPKPL-LSIQANIFDCGGMALGVCISHKAADASSLGLFLDHWAAIARGD- 517
LL F P+ IQ NIFDCGG+ + C+SHK ADA S +FL WAAI RG+
Sbjct: 121 LL---PFELDEPQEFAFGIQLNIFDCGGICIAFCLSHKIADALSTLVFLKTWAAITRGET 177
Query: 518 NEVPQPKLVQTSLFPPKDVPSYVPFGASPIQKVVNKFFVFNAGNIAALKAKAS------H 679
+++ P+ + +LFPPK++ + P + VV K FVF +I +L K S
Sbjct: 178 DDIVCPEFISATLFPPKNLSGFNPAIGITKENVVTKRFVFRLSSIESLIEKYSTSTTSIE 237
Query: 680 KSYVEHPTRVEAVSALIWRCALNASR--TRPGSVKSESAMILAMNLRGKMVPALSNLSFG 853
+ P+R+EA+S IW + A++ + PG V M+ A+NLR +M P L SFG
Sbjct: 238 NQQQKSPSRIEALSVFIWSRFMAATKVESEPGRVY---LMLHAVNLRTRMNPQLPEHSFG 294
Query: 854 NFM-IGVTATTIESAPELHCLVGELREEKMKVDDDYVRKLQVDDGLALLWNSVKDLNVRH 1030
N+ + +T + ++ E + LV +R+ K+D DYV+KLQ DG L + +K+
Sbjct: 295 NYYRVAITIPSSDTEEESYNLVRRMRDSISKIDKDYVKKLQ--DGNEHL-HFIKERAESF 351
Query: 1031 IKGELELYTTSSWCGFPFYEADFGWGKPMWISSVNLVTPNIIMLMDSKCGIGVEASVTLD 1210
+GE+ +S C FP YEADFGW KP+W + +L N+++ MD+ G G+EA + L
Sbjct: 352 TRGEMATLNFTSLCRFPLYEADFGWSKPIWAGAPSLTFKNLVVFMDTVSGDGIEALIHLK 411
Query: 1211 EEVMARFECDPELIAFTS 1264
EE MA+F+ D EL+ F +
Sbjct: 412 EEDMAKFQEDQELLQFAT 429