BLASTX nr result
ID: Coptis21_contig00001121
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00001121
(1852 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta] 713 0.0
gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver... 548 e-153
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica] 525 e-146
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 504 e-140
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum] 500 e-139
>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 534
Score = 713 bits (1840), Expect = 0.0
Identities = 354/535 (66%), Positives = 413/535 (77%)
Frame = +2
Query: 14 MQYPELHQWLPTSIIAPAPIVFSFIVIVLICIVKKRSGNVSKTRKAPEVSGAWPLVGHLN 193
M P L QW+ TS + PAPI+ SFIVI+L KKRS N+ +T+KAPEV GAWP++GHLN
Sbjct: 1 MHNPTLKQWIRTSTL-PAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLN 59
Query: 194 LVSGPKLLHFVLGELTDKYGPAFTIHLGMYPTLVVSNWELVKECFTTNDKIFSNRPVNKA 373
L+S PK + VLGEL D+YGPAF+I G++P LVVS+WELVK CFTTNDK FS+R VNKA
Sbjct: 60 LLSVPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKA 119
Query: 374 IKYMFYNEESIGFTPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWT 553
IKYMFY++++I F P+G YWRELRKM TL LLSN RL MLK RISEMDAC + LYEL T
Sbjct: 120 IKYMFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELST 179
Query: 554 KNKDDNVWVLVDMSNWFGEISFNVVARIVAGKKNFGSKGDRYKKVMEEVVRLMCVTALSD 733
K KD+N VLVDMS WFG+ISFNVV RIVAGK FG K +RY VMEE RLM V SD
Sbjct: 180 KRKDENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMDVMVFSD 239
Query: 734 IVPYXXXXXXXXXXXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTTNTTKEEVEK 913
++PY +KR AKELDS LESWV+EHRQ+RVS+SAG G N T+EE E
Sbjct: 240 VIPYLGWLDRLRGVDSEIKRTAKELDSALESWVDEHRQKRVSISAGIGGIVNITEEE-EI 298
Query: 914 DFIDITLSIMEENQLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXXHVLKKA 1093
DFIDI LSI+ +N+L GDDP T+IK+++ +M L D VLK+A
Sbjct: 299 DFIDIMLSIIAKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRA 358
Query: 1094 QRELDAQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAG 1273
Q ELDAQ+GKERQVEDSDI LPYIQAI+KE+MRLYP GPIIERE +EDCDVG F +PAG
Sbjct: 359 QCELDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAG 418
Query: 1274 TRLWVNLWKLQRDPNVWPNDPLEFQPERFLTGHADVDLRGQHLELIPFGSGRRVCPGISF 1453
TRLW+NLWKLQRDPNVWPNDP EFQPERFL GHAD+D++GQH ELIPFGSGRR+CPG+SF
Sbjct: 419 TRLWINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSF 478
Query: 1454 ALQVMHLALARIIHGFELKISNDSNIDMSGTPGIISCKATPLQVLLTPRFNPMFY 1618
+LQVMHL LARIIHGFELK D++IDMS T G+IS KATPL+VLLTPRF P+FY
Sbjct: 479 SLQVMHLVLARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPRFPPVFY 533
>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
Length = 526
Score = 548 bits (1413), Expect = e-153
Identities = 273/517 (52%), Positives = 361/517 (69%), Gaps = 6/517 (1%)
Frame = +2
Query: 68 PIVFSFIVIVLICIVKKRSGNVSKTRK-APEVSGAWPLVGHLNLVSGPKLLHFVLGELTD 244
PI + +VI L+ +R+ T+K APE SG P++GHL+L + +L H LG + D
Sbjct: 21 PISVALVVIALVWNYGRRN----PTKKLAPEASGGRPIMGHLHLFNDGELTHRKLGAMAD 76
Query: 245 KYGPAFTIHLGMYPTLVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFTPYG 424
YGP F I G + TLVVS+WE+VKECFTTNDK+FSNRP IK MFY+ +S+G+ PYG
Sbjct: 77 TYGPVFNIRFGSHKTLVVSDWEIVKECFTTNDKLFSNRPGTLGIKLMFYDADSVGYAPYG 136
Query: 425 SYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMSNWF 604
+YWR+LRK++TLKLLSNHR+D +K LR SE+++CF +LY W + + V M +W
Sbjct: 137 AYWRDLRKISTLKLLSNHRIDTIKHLRSSEVESCFESLYSQWGNGEKSGEFAPVRMDSWL 196
Query: 605 GEISFNVVARIVAGKKNFGSKGD----RYKKVMEEVVRLMCVTALSDIVPYXXXXXXXXX 772
G+++FNVVARIVAGKKNF + GD RYK M+E +RLM A SD++P
Sbjct: 197 GDLTFNVVARIVAGKKNFSANGDVGAQRYKAAMDEAMRLMRFFAFSDVIPSLSWLDNLRG 256
Query: 773 XXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTTNTTKEEVEKDFIDITLSIMEEN 952
MK+ A E+DS++ +WVEEHR +R ++G S ++E DFID+ L IME +
Sbjct: 257 LVREMKKCASEIDSIMATWVEEHRVKR---NSGGNS-------QLEHDFIDVCLDIMEHS 306
Query: 953 QLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXXHVLKKAQRELDAQIGKERQ 1132
LPGDDPD V+KS LDMILGGSD VL+KA+ EL+ Q+GK RQ
Sbjct: 307 SLPGDDPDLVVKSTCLDMILGGSDTTTVTLTWAMSLLLNHPQVLQKAKEELETQVGKNRQ 366
Query: 1133 VEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAGTRLWVNLWKLQRD 1312
V+DSDI NLP+IQAIIKE MRLYPAGP+IER EDC+V G+ VPAGTRL VN+WK+QRD
Sbjct: 367 VDDSDIPNLPFIQAIIKETMRLYPAGPLIERRTMEDCEVAGYQVPAGTRLLVNVWKMQRD 426
Query: 1313 PNVWPNDPLEFQPERFLTGHADVDLRGQHLELIPFGSGRRVCPGISFALQVMHLALARII 1492
NV+ DPLEF+P+RFLT +ADVDL+GQH ELIPFG+GRR+CPG+SFA+Q+MHL LAR++
Sbjct: 427 GNVYKGDPLEFRPDRFLTSNADVDLKGQHYELIPFGAGRRICPGVSFAVQLMHLVLARLL 486
Query: 1493 HGFEL-KISNDSNIDMSGTPGIISCKATPLQVLLTPR 1600
H FE+ + ++ +DM+ + G++ K PL+VL+ PR
Sbjct: 487 HEFEITTVEPETKVDMAESGGLLCYKIMPLEVLIKPR 523
>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
Length = 524
Score = 525 bits (1353), Expect = e-146
Identities = 262/535 (48%), Positives = 359/535 (67%), Gaps = 8/535 (1%)
Frame = +2
Query: 41 LPTSIIAPAPIVFSFIVIVLICIVKKRSGNVSKTRKAPEVSGAWPLVGHLNLVSGPKLLH 220
L + + P + + L + R+ K R AP +GAWP++GHL+L +L H
Sbjct: 4 LMLAYLFPISVASIIAFVFLYNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSGELPH 63
Query: 221 FVLGELTDKYGPAFTIHLGMYPTLVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEE 400
+L + DKYG AF + G + TLVVS+ +VKECFTTND +FSNRP KA + M Y+ E
Sbjct: 64 KMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMTYDNE 123
Query: 401 SIGFTPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWV 580
S+ FTPYGSYWRE+RK++TLKLLSNHRL +K +R SE++ CF+ LY+ KN + +
Sbjct: 124 SVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYD-QCKNPSGSAPI 182
Query: 581 LVDMSNWFGEISFNVVARIVAGKKNFGSK-------GDRYKKVMEEVVRLMCVTALSDIV 739
L+DM WF E+S NVV R++ G++NFGSK YKKVM+E++RL ++ SD
Sbjct: 183 LIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFA 242
Query: 740 PYXXXXXXXXXXXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTTNTTKEEVEKDF 919
P AMKR AK++D++LE+W+EEHR+++ SV+ E ++DF
Sbjct: 243 PLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKKNSVA------------ESQQDF 290
Query: 920 IDITLSIMEENQLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXXHVLKKAQR 1099
+D+ LSI+EE++L G D D VIK+ L MI+GG+D H LKKA+
Sbjct: 291 MDVMLSIVEESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKARE 350
Query: 1100 ELDAQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAGTR 1279
ELDA +GK+RQVEDSD+KNL Y+ AI+KE MR+YP G ++ERE EDC++ GFHV GTR
Sbjct: 351 ELDALVGKDRQVEDSDLKNLVYMNAIVKETMRMYPLGTLLERETKEDCEIDGFHVKGGTR 410
Query: 1280 LWVNLWKLQRDPNVWPNDPLEFQPERFLTGHADVDLRGQHLELIPFGSGRRVCPGISFAL 1459
L VN+WKLQRDPNVW DP EF+PERFLT +AD+D+ GQH EL+PFG+GRRVCPG+ FAL
Sbjct: 411 LLVNVWKLQRDPNVWV-DPTEFRPERFLTENADIDVGGQHFELLPFGAGRRVCPGVXFAL 469
Query: 1460 QVMHLALARIIHGFELKISNDSNIDMSGTP-GIISCKATPLQVLLTPRFNPMFYK 1621
Q MHL LAR+IHG++L N+ N+D++ +P G ++ KA+PL ++LTPR + Y+
Sbjct: 470 QFMHLVLARLIHGYDLNTLNEENVDLTESPEGHVNHKASPLDLILTPRLHYKLYE 524
>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|302142392|emb|CBI19595.3| unnamed protein product
[Vitis vinifera]
Length = 527
Score = 504 bits (1297), Expect = e-140
Identities = 270/531 (50%), Positives = 348/531 (65%), Gaps = 12/531 (2%)
Frame = +2
Query: 62 PAPIVFSFIVIVLICIV-KKRSGNVSKTRKAPEVSGAWPLVGHLNLVSG-PKLLHFVLGE 235
PA VF ++ V ++ +K+ SK+R APE GAWP++GHL+L+ G +LL+ LG
Sbjct: 8 PAFFVFLSLIYVFYAMLGRKKIIKSSKSRDAPEPGGAWPIIGHLHLLGGGDQLLYRTLGA 67
Query: 236 LTDKYGPAFTIHLGMYPTLVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFT 415
+ DKYGPAF I LG VVS+WE+ KECFT NDK + RP A K+M YN GF
Sbjct: 68 MADKYGPAFNIRLGSRRAFVVSSWEVAKECFTINDKALATRPTTVAAKHMGYNYAVFGFA 127
Query: 416 PYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMS 595
PY +WRE+RK+ TL+LLSN RL++LK +R SE+D R LY LW KN +LV+++
Sbjct: 128 PYSPFWREMRKIATLELLSNRRLEILKHVRTSEVDMGIRELYGLWVKNSSRP--LLVELN 185
Query: 596 NWFGEISFNVVARIVAGKKNFG-------SKGDRYKKVMEEVVRLMCVTALSDIVPYXXX 754
W +++ NV+ R+VAGK+ FG S+ R +K + + RL+ + +SD +P+
Sbjct: 186 RWLEDMTLNVIVRMVAGKRYFGAAAASDSSEARRCQKAISQFFRLIGIFVVSDALPF-LW 244
Query: 755 XXXXXXXXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTTNTTKEEVEKDFIDITL 934
AMK AKELDS+LE W+EEHRQRRVS + K E E+DFID+ L
Sbjct: 245 WLDLQGHERAMKTTAKELDSILEGWLEEHRQRRVS---------SLIKAEGEQDFIDVML 295
Query: 935 SIMEENQLPG--DDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXXHVLKKAQRELD 1108
S+ EE +L G D +T IKS L +ILGGSD H LKKAQ ELD
Sbjct: 296 SLQEEGRLSGFQYDSETSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHALKKAQEELD 355
Query: 1109 AQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPII-EREASEDCDVGGFHVPAGTRLW 1285
+G ERQVE+SD+KNL Y+QAIIKE +RLYPAGP++ REA +DC V G++VPAGTRL
Sbjct: 356 LCVGMERQVEESDVKNLVYLQAIIKETLRLYPAGPLLGPREALDDCTVAGYNVPAGTRLI 415
Query: 1286 VNLWKLQRDPNVWPNDPLEFQPERFLTGHADVDLRGQHLELIPFGSGRRVCPGISFALQV 1465
VN+WKLQRDP+VW N P FQPERFL HADVD++GQ EL+PFGSGRR CPG+SFALQV
Sbjct: 416 VNIWKLQRDPSVWTN-PCAFQPERFLNAHADVDVKGQQFELMPFGSGRRSCPGVSFALQV 474
Query: 1466 MHLALARIIHGFELKISNDSNIDMSGTPGIISCKATPLQVLLTPRFNPMFY 1618
+HL LAR++H FEL D +DM+ + G+ KATPL+VLLTPR N Y
Sbjct: 475 LHLTLARLLHAFELSTPVDQPVDMTESSGLTIPKATPLEVLLTPRLNSKLY 525
>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
Length = 541
Score = 500 bits (1287), Expect = e-139
Identities = 251/531 (47%), Positives = 349/531 (65%), Gaps = 18/531 (3%)
Frame = +2
Query: 80 SFIVIVLICIVKKRSGNVSKTR---------KAPEVSGAWPLVGHLNLVSGPKLLHFVLG 232
+ + +VL+ ++ + SKT + P V+GAWP+ GHL+L + H +L
Sbjct: 22 ALLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAVTGAWPVFGHLHLFGSGEHPHEMLS 81
Query: 233 ELTDKYGPAFTIHLGMYPTLVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGF 412
+L +KYGP+FT+ G + TLVVS+ +VKECFTTND +FSNRP A M Y +SI F
Sbjct: 82 KLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTNDTLFSNRPSTIAFDLMTYATDSIAF 141
Query: 413 TPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDM 592
TPY YWRELRK++TLKLLSN+RL+ +K LR SE+ CF+ LY+L K D+ V +D+
Sbjct: 142 TPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVSVCFKELYDLTNKKNDNGAPVPIDL 201
Query: 593 SNWFGEISFNVVARIVAGKKNFGSK--------GDRYKKVMEEVVRLMCVTALSDIVPYX 748
WF E+S NV+ R++ GK+NFGSK YKK+M+E+ RL +T LSD+VP
Sbjct: 202 KRWFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHYKKIMDELSRLSSLTMLSDMVPLL 261
Query: 749 XXXXXXXXXXXAMKRAAKELDSVLESWVEEHRQRRVSVSAGTGSTTNTTKEEVEKDFIDI 928
AMKR KEL+S+L+ W+EEH+ ++ S + +DF+D+
Sbjct: 262 GWLDYFKGDLRAMKRNGKELNSILQKWLEEHKSKKSS--------------DARQDFMDV 307
Query: 929 TLSIMEENQLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXXHVLKKAQRELD 1108
LSI ++ QL G D DT IK+ L MI+GG++ L KA+ E+D
Sbjct: 308 MLSISKDTQLYGHDQDTFIKATCLAMIMGGTNSTEVALTWILSLLMNNRCALHKAREEID 367
Query: 1109 AQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAGTRLWV 1288
+GK+RQVEDSD+KNL Y+ AIIKE MRLYP G ++ER+ EDC+V GF++ GTRL +
Sbjct: 368 LLVGKDRQVEDSDVKNLTYMNAIIKETMRLYPLGFLLERDTKEDCEVSGFNIKGGTRLLI 427
Query: 1289 NLWKLQRDPNVWPNDPLEFQPERFLTGHADVDLRGQHLELIPFGSGRRVCPGISFALQVM 1468
N+WKLQRDPNVW DP+EF+PERFLT +AD+D+ GQH EL+PFG+GRRVCPG+SFALQ M
Sbjct: 428 NVWKLQRDPNVW-TDPMEFKPERFLTENADIDVGGQHFELLPFGAGRRVCPGVSFALQFM 486
Query: 1469 HLALARIIHGFELKISNDSNIDMS-GTPGIISCKATPLQVLLTPRFNPMFY 1618
HL LAR+IHG++++ N ++D+S + G ++ K+TPL+++LTPR +P Y
Sbjct: 487 HLVLARLIHGYDMETLNGEDVDLSVSSGGHVNIKSTPLELILTPRLHPELY 537