BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00001076
         (1604 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   371   e-100
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    282   2e-73
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   226   1e-56
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   215   3e-53
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   213   9e-53

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  371 bits (953), Expect = e-100
 Identities = 183/198 (92%), Positives = 183/198 (92%)
 Frame = +3

Query: 759  ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIK 938
            ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIK
Sbjct: 286  ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIK 345

Query: 939  EAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFL 1118
            EAM               ETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFL
Sbjct: 346  EAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFL 405

Query: 1119 ESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMN 1298
            ESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMN
Sbjct: 406  ESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMN 465

Query: 1299 EMLSLTLAIDPSLCVVPK 1352
            EMLSLTLAIDPSLCVVPK
Sbjct: 466  EMLSLTLAIDPSLCVVPK 483


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  282 bits (722), Expect = 2e-73
 Identities = 136/198 (68%), Positives = 160/198 (80%)
 Frame = +3

Query: 759  ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIK 938
            ETFGPGSETSSAT EW +AELIK+P  +AKVR+EL++VVG ST+KESDLP LPYLQACIK
Sbjct: 146  ETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIK 205

Query: 939  EAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFL 1118
            E M               ETC VMGYTIPK++QVLVN YAIGRDPK+WK+P+TF PERFL
Sbjct: 206  ETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPERFL 265

Query: 1119 ESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMN 1298
            ESDVD++G H++FIPFG+GRR CVGMPLATRTIPLIV +LV   D+ LP G  P+DL MN
Sbjct: 266  ESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQDLVMN 325

Query: 1299 EMLSLTLAIDPSLCVVPK 1352
            E LSL+LA+DP+L V+PK
Sbjct: 326  ESLSLSLALDPTLTVIPK 343


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  226 bits (577), Expect = 1e-56
 Identities = 111/199 (55%), Positives = 142/199 (71%), Gaps = 1/199 (0%)
 Frame = +3

Query: 759  ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVG-TSTIKESDLPQLPYLQACI 935
            ETFGPGS+T+S  IEW +A+LIK+P ++AK+R+EL+ VVG +ST+KES   +LPYLQAC+
Sbjct: 286  ETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVKESHFSELPYLQACV 345

Query: 936  KEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERF 1115
            KE M               ETC+VMGYTIPK   V VNA+AIGRDPK WKDP  F PERF
Sbjct: 346  KETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDPKDWKDPLKFQPERF 405

Query: 1116 LESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKM 1295
            L+SD++++G  +QFIPFGSGRR C G PLA R IPL++ SLVH + + LP G   E L M
Sbjct: 406  LDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFGWELPDGVPNEKLDM 465

Query: 1296 NEMLSLTLAIDPSLCVVPK 1352
             E+ +L+L +   L V+PK
Sbjct: 466  EELFTLSLCMAKPLRVIPK 484


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  215 bits (547), Expect = 3e-53
 Identities = 106/198 (53%), Positives = 133/198 (67%)
 Frame = +3

Query: 759  ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIK 938
            E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG +T+KESDLP LPYLQA +K
Sbjct: 285  ELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVK 344

Query: 939  EAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFL 1118
            E +               ETC VM YTIPK  Q++VNA+AIGRDPK+W DP  F PERFL
Sbjct: 345  ETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFL 404

Query: 1119 ESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMN 1298
             SDVD+ G  ++ IPFG GRR C G+PLA++ I LIV +LV N+++ LP G    +L M+
Sbjct: 405  SSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMD 464

Query: 1299 EMLSLTLAIDPSLCVVPK 1352
            E   LTL  DP L +V K
Sbjct: 465  EKFGLTLQKDPPLLIVLK 482


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  213 bits (543), Expect = 9e-53
 Identities = 104/198 (52%), Positives = 132/198 (66%)
 Frame = +3

Query: 759  ETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIK 938
            E FG G+ETS++TIEW ++EL K+P+  A +R EL  VVG   +KESD+P +PYLQA +K
Sbjct: 281  EIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVK 340

Query: 939  EAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFL 1118
            E +               ETC+V+ YTIPK  Q++VNA+ IGRDPK W DP  F PERFL
Sbjct: 341  ETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFL 400

Query: 1119 ESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMN 1298
             S +DF G  ++ IPFG+GRR C G+PLAT+ I LIV SLV N+D+GLP G  P  L M 
Sbjct: 401  NSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGLPKGMDPSQLIME 460

Query: 1299 EMLSLTLAIDPSLCVVPK 1352
            E   LTL  +P L +VPK
Sbjct: 461  EKFGLTLQKEPPLYIVPK 478