BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000887
         (2217 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vini...   811   0.0  
ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|2...   788   0.0  
sp|P83948.1|PME3_CITSI RecName: Full=Pectinesterase 3; Short=PE ...   784   0.0  
gb|AFB77929.1| pectin methylesterase [Gossypium hirsutum]             784   0.0  
gb|AAB57670.1| pectinesterase [Citrus sinensis]                       779   0.0  

>ref|XP_002265171.1| PREDICTED: pectinesterase 3-like [Vitis vinifera]
          Length = 611

 Score =  811 bits (2095), Expect = 0.0
 Identities = 405/588 (68%), Positives = 479/588 (81%), Gaps = 7/588 (1%)
 Frame = +1

Query: 178  KMTNIKDVFAGMSDSS--CKFCKKRKFLFIIL-STXXXXXXXXXXXXXXXSRKKSND--- 339
            +M ++K  F GMSDS       K+ K LF+++ S+               SRK S +   
Sbjct: 30   QMPSVKQFFTGMSDSGKHTSISKRNKKLFLLVFSSVLLIAAVVGIVAGVHSRKSSTNDVG 89

Query: 340  -TLSHAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTI 516
             T  HA+LKSACS+TRYP+LCYSAIA+VPG + K+++QKDVI  SLN+T+TAVEHNYFTI
Sbjct: 90   LTAGHAVLKSACSSTRYPDLCYSAIATVPGASKKVTSQKDVIAVSLNITVTAVEHNYFTI 149

Query: 517  KKIIKTRKALTKREKTALHDCLEMIDETLDEVRDTEDELDDYPKKKSLNQYAEDLKTLVS 696
            +K++   K LTKREK ALHDCLE IDETLDE+    D+LD+YP KKSL Q+A+DLKTL+S
Sbjct: 150  EKLLDF-KNLTKREKAALHDCLETIDETLDELHVAMDDLDEYPDKKSLTQHADDLKTLMS 208

Query: 697  GAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSG 876
             AMTNQ++CLDGFS++ ADK VR++L+ GQ HV HM SNALAMI NMT+TD+  +R    
Sbjct: 209  AAMTNQETCLDGFSHDDADKHVREVLLKGQRHVEHMCSNALAMIKNMTDTDIAREREAMN 268

Query: 877  RNLVEEEDVNKIDSDGFPKWLSAGDRRLLQAPNVQPNVIVAADGSGNYRTXXXXXXXXPE 1056
            R L+EE D       G+PKWLSAGDRRLLQ+ +V P+V+VAADGSG+Y+T        PE
Sbjct: 269  RKLMEERD-----ESGWPKWLSAGDRRLLQSSSVTPDVVVAADGSGDYKTVSAAVAAAPE 323

Query: 1057 KSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAA 1236
            KSS RYII IKAG Y+ENVE+ KKKTNIMF+GDGR NTIITGS+NVVDGSTTF SATVA 
Sbjct: 324  KSSKRYIIGIKAGVYKENVEVGKKKTNIMFLGDGRSNTIITGSKNVVDGSTTFNSATVAV 383

Query: 1237 VGERFLARDITFQNTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCL 1416
            VGE+F+ARDITFQNTAGPSKHQAVALRVGSDLSAFY+CDMLAYQDTLYVHS RQFY+ CL
Sbjct: 384  VGEKFIARDITFQNTAGPSKHQAVALRVGSDLSAFYKCDMLAYQDTLYVHSNRQFYINCL 443

Query: 1417 VAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRADPNQNTGIVIQKCRIGATSD 1596
            VAGTVDFIFGNAAAV QDCD+HARRPNSGQ+NM TAQGR DPNQNTGIVIQKCRIGATSD
Sbjct: 444  VAGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMLTAQGRTDPNQNTGIVIQKCRIGATSD 503

Query: 1597 LLPVISSFPSYLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGA 1776
            L  VISSF +YLGRPWKEYSRT+VMQ+SI+NVI+PAGW+EW+G+FAL+TLYY EYQNTGA
Sbjct: 504  LQAVISSFKTYLGRPWKEYSRTVVMQTSITNVIDPAGWHEWSGSFALSTLYYGEYQNTGA 563

Query: 1777 GAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 1920
            GAGT+ RV W+G++V+T+A++ Q FT G FI GSSWLGSTGFP+SLGL
Sbjct: 564  GAGTSKRVTWKGFKVITSASEAQGFTPGTFIAGSSWLGSTGFPYSLGL 611


>ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|222841689|gb|EEE79236.1|
            predicted protein [Populus trichocarpa]
          Length = 579

 Score =  788 bits (2035), Expect = 0.0
 Identities = 390/583 (66%), Positives = 471/583 (80%), Gaps = 3/583 (0%)
 Frame = +1

Query: 181  MTNIKDVFAGMSDSSCK---FCKKRKFLFIILSTXXXXXXXXXXXXXXXSRKKSNDTLSH 351
            MT  K    G+SDS      F K +K   +IL++               S K S +  +H
Sbjct: 1    MTQDKQSLTGISDSGKHISIFKKNKKLFLVILASLLLVTAIIAIVAGVNSHKSSKNEGTH 60

Query: 352  AILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVEHNYFTIKKIIK 531
            AILKS+CS+T YPELCYSA+A+VPG  + +++QKDVIE S+NLT  AV+HN+FT++K+I 
Sbjct: 61   AILKSSCSSTLYPELCYSAVATVPGATSNLASQKDVIELSINLTTKAVQHNFFTVEKLIA 120

Query: 532  TRKALTKREKTALHDCLEMIDETLDEVRDTEDELDDYPKKKSLNQYAEDLKTLVSGAMTN 711
            T+K LTKREKTALHDCLE+IDETLDE+ +   +L+DYP  KSL ++A+DLKTL+S A+TN
Sbjct: 121  TKK-LTKREKTALHDCLEIIDETLDELHEALVDLNDYPNNKSLKKHADDLKTLLSSAITN 179

Query: 712  QDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSGRNLVE 891
            Q++CLDGFS+++ADK+VRK L+ GQ+HV  M SN LAMI NMT+TD+ N+   + R L++
Sbjct: 180  QETCLDGFSHDEADKKVRKALLKGQIHVEKMCSNVLAMIKNMTDTDVANELKTTNRKLMQ 239

Query: 892  EEDVNKIDSDGFPKWLSAGDRRLLQAPNVQPNVIVAADGSGNYRTXXXXXXXXPEKSSTR 1071
            E++ N+ +   +P+W+S  DRRLLQ+ +V P+V+VAADGSGNY+T        P+KSS R
Sbjct: 240  EKEGNESE---WPEWMSVADRRLLQSSSVTPDVVVAADGSGNYKTVSAAVAAAPKKSSKR 296

Query: 1072 YIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAAVGERF 1251
            YIIRIKAG YRENV++PK KTNIMFMGDGR+ TIIT SRNVVDGSTTF SATVAAVG+ F
Sbjct: 297  YIIRIKAGVYRENVDVPKDKTNIMFMGDGRKTTIITASRNVVDGSTTFNSATVAAVGQGF 356

Query: 1252 LARDITFQNTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCLVAGTV 1431
            LAR ITFQNTAGPSKHQAVALRVGSDLSAFY CDMLAYQDTLYVHS RQF++ CLVAGTV
Sbjct: 357  LARGITFQNTAGPSKHQAVALRVGSDLSAFYDCDMLAYQDTLYVHSNRQFFINCLVAGTV 416

Query: 1432 DFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRADPNQNTGIVIQKCRIGATSDLLPVI 1611
            DFIFGNAA VLQDCD+HARRPNSGQ+NM TAQGR DPNQNTGIVIQK RIGATSDL PV 
Sbjct: 417  DFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVK 476

Query: 1612 SSFPSYLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGAGTA 1791
            SSFP+YLGRPWKEYSRT++MQSSI++VI PAGW+EW+G+FALNTLYYAEYQN+GAGAGT+
Sbjct: 477  SSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWFEWSGSFALNTLYYAEYQNSGAGAGTS 536

Query: 1792 NRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 1920
             RV W+GYRV+T+A + Q FT G FI GSSWL ST FPFSLGL
Sbjct: 537  RRVTWKGYRVITSATEAQRFTPGNFIAGSSWLRSTTFPFSLGL 579


>sp|P83948.1|PME3_CITSI RecName: Full=Pectinesterase 3; Short=PE 3; AltName: Full=Pectin
            methylesterase 3; Flags: Precursor
          Length = 584

 Score =  784 bits (2025), Expect = 0.0
 Identities = 399/589 (67%), Positives = 469/589 (79%), Gaps = 9/589 (1%)
 Frame = +1

Query: 181  MTNIKDVFAGMSDSSCK-----FCKKRKFLFIIL-STXXXXXXXXXXXXXXXSRKKSNDT 342
            MT IK+ F  +S+SS         KK+K LF+ L +T               SRK S D 
Sbjct: 1    MTRIKEFFTKLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDN 60

Query: 343  LS---HAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVEHNYFT 513
             +   HAILKS+CS+TRYP+LC+SAIA+VP  + K+++QKDVIE SLN+T TAVEHNYF 
Sbjct: 61   GNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFG 120

Query: 514  IKKIIKTRKALTKREKTALHDCLEMIDETLDEVRDTEDELDDYPKKKSLNQYAEDLKTLV 693
            I+K++K R  LTKREK ALHDCLE IDETLDE+    ++L++YP KKSL+Q+A+DLKTL+
Sbjct: 121  IQKLLK-RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLM 179

Query: 694  SGAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVS 873
            S AMTNQ +CLDGFS++ A+K VR  L  GQVHV  M SNALAMI NMT+TDM   R+ +
Sbjct: 180  SAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN 239

Query: 874  GRNLVEEEDVNKIDSDGFPKWLSAGDRRLLQAPNVQPNVIVAADGSGNYRTXXXXXXXXP 1053
             R L+EE        DG+P WLS GDRRLLQ+ +V PNV+VAADGSGN++T        P
Sbjct: 240  NRKLIEETST----VDGWPAWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAP 295

Query: 1054 EKSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVA 1233
            +  + RYIIRIKAG YRENVE+ KK  NIMF+GDGR  TIITGSRNVVDGSTTFKSATVA
Sbjct: 296  QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 355

Query: 1234 AVGERFLARDITFQNTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKC 1413
             VGE FLARDITFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHS RQF+V C
Sbjct: 356  VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 415

Query: 1414 LVAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRADPNQNTGIVIQKCRIGATS 1593
            L+AGTVDFIFGNAAAVLQ+CD+HAR+PNSGQ+NM TAQGRADPNQNTGIVIQK RIGATS
Sbjct: 416  LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 475

Query: 1594 DLLPVISSFPSYLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTG 1773
            DL PV  SFP+YLGRPWKEYSRT++MQSSI++VI+PAGW+EW+GNFALNTL+Y E+QN G
Sbjct: 476  DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 535

Query: 1774 AGAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 1920
            AGAGT+ RVKW+G+RV+T+A + Q FT G FI GSSWLGSTGFPFSLGL
Sbjct: 536  AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584


>gb|AFB77929.1| pectin methylesterase [Gossypium hirsutum]
          Length = 582

 Score =  784 bits (2024), Expect = 0.0
 Identities = 395/586 (67%), Positives = 471/586 (80%), Gaps = 6/586 (1%)
 Frame = +1

Query: 181  MTNIKDVFAGMSDSS--CKFCKKRKFLFIILSTXXXXXXXXXXXXXXXSRKKSND---TL 345
            M+ IK+  + +S+S+    F KK K +F+ L                 S + ++D   T 
Sbjct: 1    MSRIKETLSNISNSAKHISFTKKHKKIFLALFASLVIVAAIIGIVAGVSSRNNSDESDTS 60

Query: 346  SHAILKSACSNTRYPELCYSAIASVP-GVAAKISTQKDVIEASLNLTITAVEHNYFTIKK 522
             HA++KSACS T YP+LC+SA+ +VP G A K+ +QKDVIE SLN+T TAVEHNYF IKK
Sbjct: 61   HHAVVKSACSGTFYPDLCFSAVTTVPAGTAKKVRSQKDVIELSLNITTTAVEHNYFKIKK 120

Query: 523  IIKTRKALTKREKTALHDCLEMIDETLDEVRDTEDELDDYPKKKSLNQYAEDLKTLVSGA 702
            ++  RK LT REKTALHDCLE IDETLDE+ +  ++L +YP KKSL Q+A+DLKTL+S A
Sbjct: 121  LL-ARKDLTTREKTALHDCLETIDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAA 179

Query: 703  MTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVSGRN 882
            MTNQ++CLDGFS+E ADK++R++LI G+ +V  M SNALAMI NMT+TD+ N+  +   N
Sbjct: 180  MTNQETCLDGFSHEGADKKIREVLIDGEKYVEKMCSNALAMIKNMTDTDIANEMMLKSSN 239

Query: 883  LVEEEDVNKIDSDGFPKWLSAGDRRLLQAPNVQPNVIVAADGSGNYRTXXXXXXXXPEKS 1062
               +ED + I    +P+WLSAGDRRLLQ+ +V PNV+VAADGSGN++T        PEKS
Sbjct: 240  RKLKEDESGI---AWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFKTVSEAVAKAPEKS 296

Query: 1063 STRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAAVG 1242
            S RYIIRIKAG YRENVE+PKKK+NIMF+GDGR  TIITGSRNVVDGSTTF SATVAAVG
Sbjct: 297  SKRYIIRIKAGVYRENVEVPKKKSNIMFIGDGRTKTIITGSRNVVDGSTTFHSATVAAVG 356

Query: 1243 ERFLARDITFQNTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKCLVA 1422
            E+FLARDITFQNTAGPSKHQAVALRVGSDLSAFY CDMLAYQDTLYVHS RQFYV CLVA
Sbjct: 357  EKFLARDITFQNTAGPSKHQAVALRVGSDLSAFYNCDMLAYQDTLYVHSNRQFYVNCLVA 416

Query: 1423 GTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRADPNQNTGIVIQKCRIGATSDLL 1602
            GTVDFIFGNAAAV Q+CD+HAR+PNSGQ+NM TAQGR DPNQNTGIVIQKCRIGATSDL 
Sbjct: 417  GTVDFIFGNAAAVFQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQ 476

Query: 1603 PVISSFPSYLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTGAGA 1782
            PV  +FP+YLGRPWKEYSRT+VMQS+IS+VI PAGW+EW+G+FAL TL+YAEYQNTGAGA
Sbjct: 477  PVRKNFPTYLGRPWKEYSRTVVMQSTISDVIQPAGWHEWSGSFALKTLFYAEYQNTGAGA 536

Query: 1783 GTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 1920
             T+ RVKW GY+V+T+A++ Q FT GRFI G SWL STGFPF+LGL
Sbjct: 537  STSARVKWGGYKVITSASEAQAFTPGRFIAGGSWLSSTGFPFALGL 582


>gb|AAB57670.1| pectinesterase [Citrus sinensis]
          Length = 584

 Score =  779 bits (2012), Expect = 0.0
 Identities = 396/589 (67%), Positives = 468/589 (79%), Gaps = 9/589 (1%)
 Frame = +1

Query: 181  MTNIKDVFAGMSDSSCK-----FCKKRKFLFIIL-STXXXXXXXXXXXXXXXSRKKSNDT 342
            MT+IK+ F  +S+SS         KK+K LF+ L +T               SRK S D 
Sbjct: 1    MTHIKEFFTKLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDN 60

Query: 343  LS---HAILKSACSNTRYPELCYSAIASVPGVAAKISTQKDVIEASLNLTITAVEHNYFT 513
             +   HAILKS+CS+TRYP+LC+SAIA+VP  + K+++QKDVIE SLN+T TAVEHNYF 
Sbjct: 61   GNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFG 120

Query: 514  IKKIIKTRKALTKREKTALHDCLEMIDETLDEVRDTEDELDDYPKKKSLNQYAEDLKTLV 693
            I+K++K R  LTKREK ALHDCLE IDETLDE+    ++L++YP KKSL+Q+A+DLKTL+
Sbjct: 121  IQKLLK-RTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLM 179

Query: 694  SGAMTNQDSCLDGFSYEKADKEVRKLLIAGQVHVYHMLSNALAMICNMTNTDMFNQRSVS 873
            S AMTNQ +CLDGFS++ A+K VR  L  GQVHV  M SNALAMI NMT+TDM   R+ +
Sbjct: 180  SAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN 239

Query: 874  GRNLVEEEDVNKIDSDGFPKWLSAGDRRLLQAPNVQPNVIVAADGSGNYRTXXXXXXXXP 1053
             R L EE        DG+P WLS GDRRLLQ+ +V PN +VAADGSGN++T        P
Sbjct: 240  NRKLTEETST----VDGWPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAP 295

Query: 1054 EKSSTRYIIRIKAGTYRENVEIPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVA 1233
            +  + RYIIRIKAG YRENVE+ KK  NIMF+GDGR  TIITGSRNVVDGSTTFKSATVA
Sbjct: 296  QGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 355

Query: 1234 AVGERFLARDITFQNTAGPSKHQAVALRVGSDLSAFYQCDMLAYQDTLYVHSLRQFYVKC 1413
             VGE FLARDITFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHS RQF+V C
Sbjct: 356  VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 415

Query: 1414 LVAGTVDFIFGNAAAVLQDCDLHARRPNSGQRNMFTAQGRADPNQNTGIVIQKCRIGATS 1593
            L+AGTVDFIFGNAAAVLQ+CD+HAR+PNSGQ+NM TAQGR DPNQNTGIVIQK RIGATS
Sbjct: 416  LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 475

Query: 1594 DLLPVISSFPSYLGRPWKEYSRTIVMQSSISNVINPAGWYEWNGNFALNTLYYAEYQNTG 1773
            DL PV  SFP+YLGRPWKEYSRT++MQSSI+++I+PAGW+EW+GNFALNTL+Y E+QN+G
Sbjct: 476  DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 535

Query: 1774 AGAGTANRVKWRGYRVLTNAADVQPFTVGRFIQGSSWLGSTGFPFSLGL 1920
            AGAGT+ RVKW+G+RV+T+A + Q FT G FI GSSWLGSTGFPFSLGL
Sbjct: 536  AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584