BLASTX nr result
ID: Coptis21_contig00000806
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00000806
(1678 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 548 e-153
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 546 e-153
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 546 e-153
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 546 e-153
ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222... 532 e-149
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 548 bits (1413), Expect = e-153
Identities = 268/452 (59%), Positives = 350/452 (77%), Gaps = 2/452 (0%)
Frame = +2
Query: 152 VIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTHDRILSARC 331
++GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA EILKTHDR+LS R
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 332 VPHVIPVYPDQIH-LSMAW-VDCSDHWRYLRTLCRTELFSAKAIASQASVRDKKTNELVE 505
VPH +P +I+ +S+ W V+C+ W+ LRT+CR ELFS K + SQA V +KK E+V
Sbjct: 113 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 506 FLAKKEGKLVSIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGMKGFLRRFVELGSTPN 685
F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++++DGGMKG +R+ V + PN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 232
Query: 686 LADFYPILSGLDLQGITKKSKEMSGQIIGVWRVITKQRREDKGNDVSRQRDFLDSLLDTE 865
L DFY I SGLDLQG+ KK+KE+ +I +W + ++RRE +D S+Q DFL+ L+ +
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ-DFLNILIRSG 291
Query: 866 LRDDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAELERETSGHIVKESEL 1045
DDQIN L EL AGADTSSST+EWAM ELIK+P++M+KV EL RE S ++ K S+L
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 1046 PHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQVLVNAWAIGRDPSTWE 1225
PHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+ VNAWAIGRDP WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 1226 DPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAKQVTLILACLINCFDWSLP 1405
DPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ + LILA L + FDWSLP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 1406 HDMLLSELDMDDKFGVTSQKKKPLVLIPKVRK 1501
+ ELDM+DKFGVT QK++PL++IPKVRK
Sbjct: 472 NGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 546 bits (1408), Expect = e-153
Identities = 266/452 (58%), Positives = 349/452 (77%), Gaps = 2/452 (0%)
Frame = +2
Query: 152 VIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTHDRILSARC 331
++GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA EILKTHDR+LS R
Sbjct: 486 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 545
Query: 332 VPHVIPVYPDQIH-LSMAW-VDCSDHWRYLRTLCRTELFSAKAIASQASVRDKKTNELVE 505
VPH +P +I+ +S+ W V+C+D W+ LRT+CR ELFS K + SQA V +KK E+V
Sbjct: 546 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 605
Query: 506 FLAKKEGKLVSIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGMKGFLRRFVELGSTPN 685
F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++ +DGGMKG +R+ + + PN
Sbjct: 606 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 665
Query: 686 LADFYPILSGLDLQGITKKSKEMSGQIIGVWRVITKQRREDKGNDVSRQRDFLDSLLDTE 865
L DFY I SGLDLQG+ KK+KE+ +I +W + ++RRE +D S+Q DFL+ L+ +
Sbjct: 666 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ-DFLNILIRSG 724
Query: 866 LRDDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAELERETSGHIVKESEL 1045
DDQIN L EL AGADTSSST+EWAM ELIK+P++M+KV EL RE S ++ K S+L
Sbjct: 725 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 784
Query: 1046 PHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQVLVNAWAIGRDPSTWE 1225
PHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+ VNAWAIGRDP WE
Sbjct: 785 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 844
Query: 1226 DPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAKQVTLILACLINCFDWSLP 1405
DPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ + LILA L + FDWSLP
Sbjct: 845 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 904
Query: 1406 HDMLLSELDMDDKFGVTSQKKKPLVLIPKVRK 1501
+ ELDM+DKFG+ QK++PL++IPKVRK
Sbjct: 905 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 546 bits (1408), Expect = e-153
Identities = 266/452 (58%), Positives = 349/452 (77%), Gaps = 2/452 (0%)
Frame = +2
Query: 152 VIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTHDRILSARC 331
++GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA EILKTHDR+LS R
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 332 VPHVIPVYPDQIH-LSMAW-VDCSDHWRYLRTLCRTELFSAKAIASQASVRDKKTNELVE 505
VPH +P +I+ +S+ W V+C+D W+ LRT+CR ELFS K + SQA V +KK E+V
Sbjct: 113 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 506 FLAKKEGKLVSIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGMKGFLRRFVELGSTPN 685
F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++ +DGGMKG +R+ + + PN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 232
Query: 686 LADFYPILSGLDLQGITKKSKEMSGQIIGVWRVITKQRREDKGNDVSRQRDFLDSLLDTE 865
L DFY I SGLDLQG+ KK+KE+ +I +W + ++RRE +D S+Q DFL+ L+ +
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ-DFLNILIRSG 291
Query: 866 LRDDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAELERETSGHIVKESEL 1045
DDQIN L EL AGADTSSST+EWAM ELIK+P++M+KV EL RE S ++ K S+L
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 1046 PHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQVLVNAWAIGRDPSTWE 1225
PHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+ VNAWAIGRDP WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 1226 DPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAKQVTLILACLINCFDWSLP 1405
DPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ + LILA L + FDWSLP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 1406 HDMLLSELDMDDKFGVTSQKKKPLVLIPKVRK 1501
+ ELDM+DKFG+ QK++PL++IPKVRK
Sbjct: 472 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 546 bits (1407), Expect = e-153
Identities = 267/452 (59%), Positives = 349/452 (77%), Gaps = 2/452 (0%)
Frame = +2
Query: 152 VIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTHDRILSARC 331
++GN+L++GN PHISLAR +++YGPLISLRLG+Q++VV S+ +AA EILKTHDR+LS R
Sbjct: 617 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 676
Query: 332 VPHVIPVYPDQIH-LSMAW-VDCSDHWRYLRTLCRTELFSAKAIASQASVRDKKTNELVE 505
VPH +P +I+ +S+ W V+C+ W+ LRT+CR ELFS K + SQA V +KK E+V
Sbjct: 677 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 736
Query: 506 FLAKKEGKLVSIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGMKGFLRRFVELGSTPN 685
F++ KEG+++ +GEVVFATV N L+ + +S+DFIS E++++DGGMKG +R+ V + PN
Sbjct: 737 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 796
Query: 686 LADFYPILSGLDLQGITKKSKEMSGQIIGVWRVITKQRREDKGNDVSRQRDFLDSLLDTE 865
L DFY I SGLDLQG+ KK+KE+ +I +W + ++RRE +D S+Q DFL+ L+ +
Sbjct: 797 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERREGASDDPSKQ-DFLNILIRSG 855
Query: 866 LRDDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAELERETSGHIVKESEL 1045
DDQIN L EL AGADTSSST+EWAM ELIK+P++M+KV EL RE S ++ K S+L
Sbjct: 856 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 915
Query: 1046 PHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQVLVNAWAIGRDPSTWE 1225
PHLPYL + V ETLRLHP AP LLP RA +C+V+NYTIPKDSQ+ VNAWAIGRDP WE
Sbjct: 916 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 975
Query: 1226 DPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAKQVTLILACLINCFDWSLP 1405
DPL FKPERFL+S +D +N +FIPFGAGRRICPGLPMAA+ + LILA L + FDWSLP
Sbjct: 976 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 1035
Query: 1406 HDMLLSELDMDDKFGVTSQKKKPLVLIPKVRK 1501
+ ELDM+DKFGVT QK++PL++IPK RK
Sbjct: 1036 NGTTPDELDMNDKFGVTLQKEQPLLIIPKKRK 1067
>ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222859506|gb|EEE97053.1|
cytochrome P450 [Populus trichocarpa]
Length = 503
Score = 532 bits (1371), Expect = e-149
Identities = 265/451 (58%), Positives = 339/451 (75%), Gaps = 2/451 (0%)
Frame = +2
Query: 152 VIGNILNMGNKPHISLARLAKAYGPLISLRLGTQLVVVGSSQAAATEILKTHDRILSARC 331
+IGN+L++GN+PH+SLA +AK +GPLISLRLGTQL+VVGSS AA EILKTHDR LSAR
Sbjct: 53 IIGNLLHVGNQPHVSLAEIAKIHGPLISLRLGTQLLVVGSSAKAAAEILKTHDRFLSARH 112
Query: 332 VPHVIPVYPDQIH-LSMAWVDCS-DHWRYLRTLCRTELFSAKAIASQASVRDKKTNELVE 505
VP VIP + +++ W S D W+ LR LCRTELFSAKAI S A++R+KK EL++
Sbjct: 113 VPQVIPRESHVLRRVALVWCPESIDTWKLLRGLCRTELFSAKAIESSATLREKKVGELMD 172
Query: 506 FLAKKEGKLVSIGEVVFATVFNMLSNIFVSKDFISLEEESEDGGMKGFLRRFVELGSTPN 685
FL +EGK+VSIGEVVF+TVFN +SN+ S D LEE+ G+K +R+ + L +TPN
Sbjct: 173 FLVAREGKVVSIGEVVFSTVFNTISNLLFSNDLAGLEEKGMSSGLKSHVRKLMLLVATPN 232
Query: 686 LADFYPILSGLDLQGITKKSKEMSGQIIGVWRVITKQRREDKGNDVSRQRDFLDSLLDTE 865
+ADFYPI +GLD QG+ +K ++ + +W + K+RR +D S +RDFLD L
Sbjct: 233 IADFYPIFAGLDPQGLRRKLSKLVEETFAIWAINIKERRNSYVHD-SPKRDFLDVFLANG 291
Query: 866 LRDDQINYLIQELFLAGADTSSSTVEWAMTELIKNPKAMRKVCAELERETSGHIVKESEL 1045
DDQIN+L ELF AG DT+++T+EWA+ E++KN + M+KV ELERE + + + ES++
Sbjct: 292 FDDDQINWLAAELFSAGTDTTATTIEWAVAEILKNKEVMKKVDEELEREITKNTISESDV 351
Query: 1046 PHLPYLHSTVNETLRLHPPAPFLLPHRALETCKVLNYTIPKDSQVLVNAWAIGRDPSTWE 1225
LPYL++ + ETLRLHPP P L+PHRA ETC+V+ YTIPKDSQVLVN WAI RDPSTWE
Sbjct: 352 SGLPYLNACIKETLRLHPPVPLLVPHRATETCEVMKYTIPKDSQVLVNVWAISRDPSTWE 411
Query: 1226 DPLSFKPERFLDSGLDLNSSNFKFIPFGAGRRICPGLPMAAKQVTLILACLINCFDWSLP 1405
DPLSFKP+RFL S L+ N++F+PFGAGRRICPGLPMA K V LILA LI CFDWSLP
Sbjct: 412 DPLSFKPDRFLGSNLEFKGGNYEFLPFGAGRRICPGLPMANKLVPLILASLIRCFDWSLP 471
Query: 1406 HDMLLSELDMDDKFGVTSQKKKPLVLIPKVR 1498
+ L++LDM DKFGV QK++PLVL+PK R
Sbjct: 472 NGEDLAKLDMKDKFGVVLQKEQPLVLVPKRR 502