BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00000729
         (1869 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       525   e-146
gb|ABC59099.1| cytochrome P450 monooxygenase CYP72A66 [Medicago ...   325   3e-86
ref|XP_002333744.1| cytochrome P450 [Populus trichocarpa] gi|222...   323   7e-86
dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza urale...   323   1e-85
ref|XP_002334834.1| cytochrome P450 [Populus trichocarpa] gi|222...   322   2e-85

>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  525 bits (1352), Expect = e-146
 Identities = 255/255 (100%), Positives = 255/255 (100%)
 Frame = +2

Query: 347  MVGGILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDI 526
            MVGGILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDI
Sbjct: 1    MVGGILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDI 60

Query: 527  VSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILS 706
            VSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILS
Sbjct: 61   VSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILS 120

Query: 707  NKFGHFEKIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEM 886
            NKFGHFEKIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEM
Sbjct: 121  NKFGHFEKIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEM 180

Query: 887  VNKWEMMVSQDSCELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQ 1066
            VNKWEMMVSQDSCELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQ
Sbjct: 181  VNKWEMMVSQDSCELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQ 240

Query: 1067 SIYIPGLRFLPTKRN 1111
            SIYIPGLRFLPTKRN
Sbjct: 241  SIYIPGLRFLPTKRN 255



 Score =  426 bits (1096), Expect = e-117
 Identities = 208/208 (100%), Positives = 208/208 (100%)
 Frame = +3

Query: 1224 FGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFD 1403
            FGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFD
Sbjct: 311  FGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFD 370

Query: 1404 GLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELW 1583
            GLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELW
Sbjct: 371  GLNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELW 430

Query: 1584 GEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFE 1763
            GEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFE
Sbjct: 431  GEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFE 490

Query: 1764 LSSTYVHAPYTVITLQPQHGAQLILHKL 1847
            LSSTYVHAPYTVITLQPQHGAQLILHKL
Sbjct: 491  LSSTYVHAPYTVITLQPQHGAQLILHKL 518


>gb|ABC59099.1| cytochrome P450 monooxygenase CYP72A66 [Medicago truncatula]
          Length = 395

 Score =  325 bits (832), Expect = 3e-86
 Identities = 150/207 (72%), Positives = 180/207 (86%)
 Frame = +3

Query: 1227 GMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDG 1406
            GM++D V++ECKLF+FAGQETTS LLVWTM++LS +PDWQ +AREEVL VFG K PDFDG
Sbjct: 187  GMSLDHVVEECKLFHFAGQETTSVLLVWTMILLSRYPDWQTRAREEVLHVFGNKKPDFDG 246

Query: 1407 LNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWG 1586
            LN+LKIVTMILYEVLRLYPP + +AR   K MKLG +TLPAGV + +P LL+HHD +LWG
Sbjct: 247  LNNLKIVTMILYEVLRLYPPVMGLARNVVKDMKLGNLTLPAGVEVFLPILLIHHDCKLWG 306

Query: 1587 EDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFEL 1766
            +DA+ FNPERFSGG+SKAT  +VSFFPF WGPRICIGQNF+L+E+KMA++MILQ FSFEL
Sbjct: 307  DDAKMFNPERFSGGISKATNGRVSFFPFGWGPRICIGQNFSLMEAKMALSMILQHFSFEL 366

Query: 1767 SSTYVHAPYTVITLQPQHGAQLILHKL 1847
            SSTY HAP TVITLQPQ+GA +I+ K+
Sbjct: 367  SSTYAHAPSTVITLQPQYGAHIIIRKV 393



 Score =  160 bits (404), Expect = 1e-36
 Identities = 76/116 (65%), Positives = 92/116 (79%), Gaps = 1/116 (0%)
 Frame = +2

Query: 755  LLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEMVNKWEMMVSQD-SCEL 931
            LL SG+ SY GEKW KHRR++NPAFHLE+LK+M PA +TSC++M++KWE M+S D SCE+
Sbjct: 11   LLASGLASYGGEKWSKHRRLINPAFHLEQLKIMTPAFFTSCNDMISKWEEMLSSDGSCEI 70

Query: 932  DVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQSIYIPGLRFLP 1099
            DVWP LQNL +D ISRTAFGSSYEEG+RIFQLQ EQA L+   +    IP   FLP
Sbjct: 71   DVWPSLQNLASDAISRTAFGSSYEEGKRIFQLQREQAELITTVVMKSTIPLWMFLP 126


>ref|XP_002333744.1| cytochrome P450 [Populus trichocarpa] gi|222838385|gb|EEE76750.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 518

 Score =  323 bits (829), Expect = 7e-86
 Identities = 145/206 (70%), Positives = 181/206 (87%)
 Frame = +3

Query: 1230 MTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDGL 1409
            M+I++VIDECK+FYFAGQETTS LL WTMV+L+ +P+WQ +AREEV+QVFG K PDFDGL
Sbjct: 313  MSIEDVIDECKIFYFAGQETTSVLLTWTMVLLAQYPNWQARAREEVVQVFGNKKPDFDGL 372

Query: 1410 NHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWGE 1589
            NHLK+VTMILYEVLRLYPP +++ R  ++ +KLG + LPAGV +++PT+L+H D ELWG+
Sbjct: 373  NHLKVVTMILYEVLRLYPPVIMLNRDVHEEIKLGNLLLPAGVQISLPTILLHQDHELWGD 432

Query: 1590 DAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFELS 1769
            DA +F PERF+ GVSKATK+QVSF PF WGPRIC+GQNFAL+E+KMA+AM+LQR+SFELS
Sbjct: 433  DASEFKPERFAEGVSKATKSQVSFLPFGWGPRICVGQNFALIEAKMALAMVLQRYSFELS 492

Query: 1770 STYVHAPYTVITLQPQHGAQLILHKL 1847
             +Y+HAP TVITLQPQHGA +IL KL
Sbjct: 493  PSYIHAPRTVITLQPQHGAPMILRKL 518



 Score =  276 bits (707), Expect = 1e-71
 Identities = 134/251 (53%), Positives = 175/251 (69%), Gaps = 3/251 (1%)
 Frame = +2

Query: 368  TLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDIVSATQEA 547
            ++LV+   + +  WAW+   WVW  P+K+E+CLR+QG  G  Y+ L G+ K+     +EA
Sbjct: 7    SILVSIVYVAVLRWAWRVLNWVWFRPKKVERCLRQQGFAGKPYRLLFGDWKENSDMLKEA 66

Query: 548  RAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILSNKFGHFE 727
            R KP+ LS  ++PRVMPF+HQ V+ YGK SF+W GP  RVNIM+PD IRD+   K   ++
Sbjct: 67   RTKPIGLSDALLPRVMPFLHQLVKDYGKNSFMWIGPKPRVNIMNPDQIRDVFM-KINEYQ 125

Query: 728  KIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEMVNKWEMM 907
            K  ++PL K L  G+ S+EGEKW KHR+I+NPAFH EKLK+M+PA Y SCS M+NKWE +
Sbjct: 126  K-PSHPLLKPLACGLASHEGEKWAKHRKIINPAFHQEKLKLMIPAFYESCSGMINKWEKL 184

Query: 908  VSQD--SCELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKA-LQSIYI 1078
            VS D  SCELDVWP LQ LT D ISRT+FGS+YEEG+RIF L  E   L +   +++  I
Sbjct: 185  VSVDEGSCELDVWPDLQGLTCDAISRTSFGSNYEEGKRIFDLLKELTDLTVHVIIKAFVI 244

Query: 1079 PGLRFLPTKRN 1111
            PG RFLP   N
Sbjct: 245  PGYRFLPIPTN 255


>dbj|BAL45206.1| cytochrome P450 monooxygenase [Glycyrrhiza uralensis]
          Length = 522

 Score =  323 bits (828), Expect = 1e-85
 Identities = 152/207 (73%), Positives = 178/207 (85%)
 Frame = +3

Query: 1227 GMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDG 1406
            GM++++VI+ECKLFYFAGQETTS LLVWTMV+LS +PDWQ +AREEVLQVFG K PDFDG
Sbjct: 314  GMSLEDVIEECKLFYFAGQETTSGLLVWTMVLLSRYPDWQARAREEVLQVFGNKKPDFDG 373

Query: 1407 LNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWG 1586
            L+HLKIVTMILYEVLRLYPP + +AR  +K +KLG  TLPAGV L +P +L HHDRELWG
Sbjct: 374  LSHLKIVTMILYEVLRLYPPVIALARTVHKDVKLGNRTLPAGVQLYLPIVLFHHDRELWG 433

Query: 1587 EDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFEL 1766
            +DAE FNPERFS GV KAT  +VSFFPF WGPRIC+GQNF+LLE+KMA++MILQ FSFEL
Sbjct: 434  DDAEVFNPERFSEGVLKATNGKVSFFPFGWGPRICVGQNFSLLEAKMAVSMILQHFSFEL 493

Query: 1767 SSTYVHAPYTVITLQPQHGAQLILHKL 1847
            S  Y HAP TVITL+PQ+GA +IL K+
Sbjct: 494  SPAYAHAPTTVITLRPQYGAHIILRKV 520



 Score =  294 bits (753), Expect = 5e-77
 Identities = 138/249 (55%), Positives = 184/249 (73%), Gaps = 1/249 (0%)
 Frame = +2

Query: 359  ILFTLLVTCAVIYISSWAWKAFVWVWLNPRKLEKCLREQGIKGPSYKFLSGNLKDIVSAT 538
            +  T  +T  ++   +WAW+   W+WL P+KLE+ LREQG++G  Y+ + G+LKD++   
Sbjct: 7    LAITAGITLVIVLAVTWAWRMLNWLWLRPKKLERLLREQGLQGNPYRLIVGDLKDLMKMR 66

Query: 539  QEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPYCRVNIMDPDMIRDILSNKFG 718
            +EA++KPM LS  I+PRV P+V Q+V K+GK SFIW+GP  RV + DP+ I+D+L NK  
Sbjct: 67   KEAKSKPMNLSDDILPRVFPYVQQSVTKHGKNSFIWFGPIPRVTLTDPEQIKDVL-NKIY 125

Query: 719  HFEKIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLEKLKMMLPAIYTSCSEMVNKW 898
             F K   + L KLL +G+VSYEGEKW KHRRI+NPAF+LEKLK+MLP  + SC++++ KW
Sbjct: 126  DFPKSDGDRLVKLLATGLVSYEGEKWSKHRRIINPAFNLEKLKIMLPIFFQSCNDLITKW 185

Query: 899  EMMVSQD-SCELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQLQSEQAGLVIKALQSIY 1075
            E M+S D SCE+D+WP LQNL +DVI+R AFGSSYEEGRRIFQLQ EQ    +  L  +Y
Sbjct: 186  EGMLSSDGSCEMDIWPFLQNLASDVIARAAFGSSYEEGRRIFQLQKEQVEHTMSVLVKVY 245

Query: 1076 IPGLRFLPT 1102
            IPG RFLPT
Sbjct: 246  IPGWRFLPT 254


>ref|XP_002334834.1| cytochrome P450 [Populus trichocarpa] gi|222875161|gb|EEF12292.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 353

 Score =  322 bits (825), Expect = 2e-85
 Identities = 145/207 (70%), Positives = 180/207 (86%)
 Frame = +3

Query: 1227 GMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGKDPDFDG 1406
            GM+I++VIDECK+FYFAGQETT  LL WTMV+L+ +P+WQ +AREEV+QVFG K PDFDG
Sbjct: 147  GMSIEDVIDECKIFYFAGQETTLVLLTWTMVLLAQYPNWQARAREEVVQVFGNKKPDFDG 206

Query: 1407 LNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVHHDRELWG 1586
            LNHLK+VTMILYEVLRLYPP + + R  ++ +KLG + LPAGV +++PT+L+H D ELWG
Sbjct: 207  LNHLKVVTMILYEVLRLYPPVITLNRDVHEEIKLGNLLLPAGVQVSLPTILLHQDHELWG 266

Query: 1587 EDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKMAIAMILQRFSFEL 1766
            +DA +F PERF+ GVSKATK+QVSF PF WGPRIC+GQNFAL+E+KMA+AM+LQR+SFEL
Sbjct: 267  DDASEFKPERFAEGVSKATKSQVSFLPFGWGPRICVGQNFALIEAKMALAMVLQRYSFEL 326

Query: 1767 SSTYVHAPYTVITLQPQHGAQLILHKL 1847
            S +Y+HAP TVITLQPQHGA +IL KL
Sbjct: 327  SPSYIHAPRTVITLQPQHGAPMILRKL 353



 Score =  105 bits (262), Expect = 4e-20
 Identities = 53/90 (58%), Positives = 62/90 (68%), Gaps = 3/90 (3%)
 Frame = +2

Query: 851  MLPAIYTSCSEMVNKWEMMVSQDS--CELDVWPHLQNLTADVISRTAFGSSYEEGRRIFQ 1024
            M+PA Y SCS M+NKWE +VS D   CELDVWP LQ LT D ISRT+FGS+YEEG+RIF 
Sbjct: 1    MIPAFYESCSGMINKWEKLVSVDEGPCELDVWPDLQGLTCDAISRTSFGSNYEEGKRIFD 60

Query: 1025 LQSEQAGLVIKA-LQSIYIPGLRFLPTKRN 1111
            L  E   L     +++I IPG RFLP   N
Sbjct: 61   LLKELTNLTAHVIIKAIVIPGYRFLPIPSN 90