BLASTX nr result
ID: Coptis21_contig00000531
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis21_contig00000531
(1357 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 492 e-136
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 462 e-128
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 458 e-126
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 435 e-119
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica] 401 e-109
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT gi|226897702|gb|ACO90222.1|
tetrahydroprotoberberine N-methyltransferase
[Eschscholzia californica]
Length = 350
Score = 492 bits (1266), Expect = e-136
Identities = 224/345 (64%), Positives = 293/345 (84%)
Frame = +1
Query: 70 SAMQILDRLDNGLIPDEELRKLVLIQFKRRLQWGYKPTYAQQLEFNLNFIKSLKQMKMSG 249
SA +I+ RL G I DEEL+KL+ Q+ RR++WGYKPT+ +QL FNL+FIK LK+M MSG
Sbjct: 4 SAGEIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMSG 63
Query: 250 TMDVINSESYDLPAAFLEAMFGQTLKQSFCYFEDESTTLDEAETATHDLYCERAQIQDGQ 429
+D +N E+Y+LP AFLEA+FG+T+KQS CYF+DE++T+DEAE A H+LYCERAQI+DGQ
Sbjct: 64 EIDTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDGQ 123
Query: 430 KILDIGCGQGSFVIHVAQKFKSCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTKY 609
+LDIGCGQG V+++A+K+K+CHVTG+TNS+AQ + IE++ + LEL+NVDVI ADVTK+
Sbjct: 124 TVLDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKF 183
Query: 610 DTNETFDRIIVIEAVEHMKNVGLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEEDW 789
DT++T+DRI+V+E +EHMKN+ LF++K+S WMTED LLF+D+I HKTF H+FEA+DE+DW
Sbjct: 184 DTDKTYDRILVVETIEHMKNIQLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDW 243
Query: 790 YSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAVRKI 969
YS +IFPKG VTI +STLLYFQDDV+ +D WV NG HMAR+VE W KLD+ I+A R+I
Sbjct: 244 YSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREI 303
Query: 970 LEPCLGSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFR 1104
LEP LGSK+AV++VITH RTFCIG YEQFS N+G+EWMI+ ILF+
Sbjct: 304 LEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFK 348
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
tetrahydroprotoberberine N-methyltransferase [Papaver
bracteatum]
Length = 358
Score = 462 bits (1189), Expect = e-128
Identities = 210/348 (60%), Positives = 286/348 (82%), Gaps = 1/348 (0%)
Frame = +1
Query: 64 KVSAMQILDRLDNGLIPDEELRKLVLIQFKRRLQWGYKPTYAQQLEFNLNFIKSLKQMKM 243
K SA + L RL G I DEEL+KL+ QF++RLQWGYK ++ +QL FNL+FIKSLK+M+M
Sbjct: 9 KESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEM 68
Query: 244 SGTMDVINSESYDLPAAFLEAMFGQTLKQSFCYFEDESTTLDEAETATHDLYCERAQIQD 423
SG ++ +N E+Y+LP+ FLEA+FG+T+KQS CYF+ ES T+DEAE A H+LYCERAQI+D
Sbjct: 69 SGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATIDEAEEAAHELYCERAQIKD 128
Query: 424 GQKILDIGCGQGSFVIHVAQKFKSCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVT 603
GQ +LDIGCGQG V+++A+K+K CHVTG+TNS+AQ + + ++ + L L+NVD ILADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVT 188
Query: 604 KYDTNETFDRIIVIEAVEHMKNVGLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEE 783
+Y++++T+DR+++IEA+EHMKN+ LF++K+S WMTE+ LLF+D++CHKTF H FEA+DE+
Sbjct: 189 QYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHKTFAHFFEAVDED 248
Query: 784 DWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAVR 963
DWYS +IFP G TI A++LLYFQDDV+VVD WV NG HMAR+V+ W LDKN++A +
Sbjct: 249 DWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAK 308
Query: 964 KILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFR 1104
+IL P L GS +AV+ V+TH RTFC+G YEQFS NDG EWM++ +LF+
Sbjct: 309 EILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFK 356
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-
N-methyltransferase [Papaver somniferum]
Length = 358
Score = 458 bits (1179), Expect = e-126
Identities = 208/348 (59%), Positives = 285/348 (81%), Gaps = 1/348 (0%)
Frame = +1
Query: 64 KVSAMQILDRLDNGLIPDEELRKLVLIQFKRRLQWGYKPTYAQQLEFNLNFIKSLKQMKM 243
K SA + L RL G I DEEL+KL+ QF++RLQWGYK ++ +QL FNL+FIKSLK+M+M
Sbjct: 9 KESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLKKMEM 68
Query: 244 SGTMDVINSESYDLPAAFLEAMFGQTLKQSFCYFEDESTTLDEAETATHDLYCERAQIQD 423
SG ++ +N E+Y+LP+ FLEA+FG+T+KQS CYF ES T+DEAE A H+LYCERAQI+D
Sbjct: 69 SGEIETMNKETYELPSEFLEAVFGKTVKQSMCYFTHESATIDEAEEAAHELYCERAQIKD 128
Query: 424 GQKILDIGCGQGSFVIHVAQKFKSCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVT 603
GQ +LDIGCGQG V+++AQK+K+CHVTG+TNS+AQ + + ++ + L L+NVD ILADVT
Sbjct: 129 GQTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVT 188
Query: 604 KYDTNETFDRIIVIEAVEHMKNVGLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEE 783
+Y++++T+DR+++IEA+EHMKN+ LF++K+S WMT++ LLF+D++CHKTF H FEA+DE+
Sbjct: 189 QYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTKESLLFVDHVCHKTFAHFFEAVDED 248
Query: 784 DWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAVR 963
DWYS +IFP G TI A++LLYFQDDV+VVD WV NG HMAR+V+ W LDKN++A +
Sbjct: 249 DWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAK 308
Query: 964 KILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFR 1104
+IL P L GS + V+ V+TH RTFC+G YEQFS N+G EWM++ +LF+
Sbjct: 309 EILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLFK 356
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2
gi|226897730|gb|ACO90236.1| putative N-methyltransferase
[Papaver bracteatum]
Length = 358
Score = 435 bits (1119), Expect = e-119
Identities = 208/356 (58%), Positives = 272/356 (76%), Gaps = 1/356 (0%)
Frame = +1
Query: 40 MDSRHVSNKVSAMQILDRLDNGLIPDEELRKLVLIQFKRRLQWGYKPTYAQQLEFNLNFI 219
M S K SA + L RL G I DEEL+KL+ Q ++RLQWGYK ++ +QL FNL+FI
Sbjct: 1 MGSIEEVKKESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFI 60
Query: 220 KSLKQMKMSGTMDVINSESYDLPAAFLEAMFGQTLKQSFCYFEDESTTLDEAETATHDLY 399
SLK+M MSG ++ +E Y+LP EA +G+++K S CYF+ ES+T+DEAE A+H+LY
Sbjct: 61 NSLKKMGMSGQVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELY 120
Query: 400 CERAQIQDGQKILDIGCGQGSFVIHVAQKFKSCHVTGITNSRAQKSIIEERCKNLELSNV 579
CERAQI+DGQ +LDIGCGQG V++VAQK+K+CHVTG+TNS+ Q + I ++ + L L NV
Sbjct: 121 CERAQIKDGQTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNV 180
Query: 580 DVILADVTKYDTNETFDRIIVIEAVEHMKNVGLFLRKISKWMTEDGLLFLDYICHKTFGH 759
DVILADVT+Y++++T+DRI+VI VEHMKN+ LF++K+S WM ED LLF+D+ CHKTF H
Sbjct: 181 DVILADVTQYESDKTYDRILVIGVVEHMKNMQLFIKKLSTWMAEDSLLFVDHSCHKTFNH 240
Query: 760 HFEAIDEEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKL 939
FEA+DE+DWYS YIFP G T A +LLYFQDDV+VVD WV NG H ARTV+ W KL
Sbjct: 241 FFEALDEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKL 300
Query: 940 DKNIDAVRKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFR 1104
DKN++AV++IL P L G+ +AV+ VITH RT C+G Y QFS NDG EWM + +LF+
Sbjct: 301 DKNMEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLFK 356
>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 401 bits (1030), Expect = e-109
Identities = 178/349 (51%), Positives = 260/349 (74%)
Frame = +1
Query: 58 SNKVSAMQILDRLDNGLIPDEELRKLVLIQFKRRLQWGYKPTYAQQLEFNLNFIKSLKQM 237
+ K + +++L +L+ GL+P +++++L+ + RRLQWGYKPTY +Q+ N SL+QM
Sbjct: 8 TKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHSLRQM 67
Query: 238 KMSGTMDVINSESYDLPAAFLEAMFGQTLKQSFCYFEDESTTLDEAETATHDLYCERAQI 417
K++ ++ ++S+ Y++P FL+ M G LK S CYF+++STTLDEAE A DLYCERAQI
Sbjct: 68 KIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQI 127
Query: 418 QDGQKILDIGCGQGSFVIHVAQKFKSCHVTGITNSRAQKSIIEERCKNLELSNVDVILAD 597
QDGQ +LD+GCGQG+ +HVAQK+K+C VT +TNS +QK IEE + L NV+V LAD
Sbjct: 128 QDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLAD 187
Query: 598 VTKYDTNETFDRIIVIEAVEHMKNVGLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAID 777
+T ++ ET+DRI+VIE EHMKN L LRKIS+W+++DGLLFL++ICHKTF +H+E +D
Sbjct: 188 ITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLD 247
Query: 778 EEDWYSNYIFPKGSVTIPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDA 957
++DW++ Y+FP G++ IP AS LYFQDDV+VV+ W +GKH +RT EEWL +LD N+D
Sbjct: 248 DDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDV 307
Query: 958 VRKILEPCLGSKDAVDKVITHSRTFCIGAYEQFSCNDGQEWMISHILFR 1104
++ + E +G+++ K+I + R FC+ E F N+G+EWM SH+LF+
Sbjct: 308 IKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFK 356