BLASTX nr result
ID: Cocculus23_contig00051488
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus23_contig00051488
(329 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006480387.1| PREDICTED: uncharacterized protein LOC102611... 199 3e-49
gb|ABG37668.1| gag protein [Populus trichocarpa] 181 7e-44
gb|ABG37657.1| integrase [Populus trichocarpa] 181 7e-44
gb|ABG37656.1| integrase [Populus trichocarpa] 181 1e-43
gb|ABG37671.1| integrase [Populus trichocarpa] 179 3e-43
gb|ABG37670.1| integrase [Populus trichocarpa] 176 4e-42
gb|ABD63142.1| Retrotransposon gag protein [Asparagus officinalis] 174 2e-41
gb|AHN65359.1| integrase [Glycine max] 173 2e-41
ref|XP_006471184.1| PREDICTED: uncharacterized protein LOC102621... 167 2e-39
ref|XP_004515949.1| PREDICTED: uncharacterized protein LOC101504... 166 4e-39
ref|XP_006575971.1| PREDICTED: uncharacterized protein LOC102659... 165 5e-39
emb|CAN78799.1| hypothetical protein VITISV_038948 [Vitis vinifera] 163 2e-38
emb|CAN81490.1| hypothetical protein VITISV_031325 [Vitis vinifera] 163 2e-38
emb|CAN79155.1| hypothetical protein VITISV_025696 [Vitis vinifera] 163 2e-38
emb|CAN81739.1| hypothetical protein VITISV_009673 [Vitis vinifera] 163 2e-38
emb|CAN65053.1| hypothetical protein VITISV_033133 [Vitis vinifera] 163 2e-38
emb|CAN73555.1| hypothetical protein VITISV_021948 [Vitis vinifera] 163 2e-38
emb|CAN76883.1| hypothetical protein VITISV_036351 [Vitis vinifera] 163 2e-38
emb|CAN65863.1| hypothetical protein VITISV_015140 [Vitis vinifera] 163 2e-38
emb|CAN62600.1| hypothetical protein VITISV_027792 [Vitis vinifera] 163 2e-38
>ref|XP_006480387.1| PREDICTED: uncharacterized protein LOC102611294 [Citrus sinensis]
Length = 1430
Score = 199 bits (507), Expect = 3e-49
Identities = 91/109 (83%), Positives = 102/109 (93%)
Frame = +1
Query: 1 TPKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNP 180
TPKAIISDRGTHFCNR VEAL +KYNITH+ ST+YHPQTSGQ EVSNRE+KSILEKTVNP
Sbjct: 1207 TPKAIISDRGTHFCNRSVEALFRKYNITHKVSTSYHPQTSGQVEVSNREVKSILEKTVNP 1266
Query: 181 NRKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
NRKDWS+RLDDAL AY+TA KT IGMSPYRL++GKPCHLP+ELEH+A+W
Sbjct: 1267 NRKDWSLRLDDALWAYRTAYKTPIGMSPYRLVYGKPCHLPVELEHKAWW 1315
>gb|ABG37668.1| gag protein [Populus trichocarpa]
Length = 1886
Score = 181 bits (460), Expect = 7e-44
Identities = 79/108 (73%), Positives = 97/108 (89%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+A+ISD GTHFCN+ E+L+KKY ITH+ +T YHPQTSGQ E++NREIK ILEKTVNPN
Sbjct: 1016 PRAMISDGGTHFCNKPFESLMKKYGITHKVATPYHPQTSGQVELANREIKQILEKTVNPN 1075
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL+DAL AY+TA KT +GMSPYRL++GKPCHLP+E+EH+AYW
Sbjct: 1076 RKDWSLRLNDALWAYRTAYKTSLGMSPYRLVYGKPCHLPVEIEHKAYW 1123
Score = 71.6 bits (174), Expect = 1e-10
Identities = 31/53 (58%), Positives = 41/53 (77%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSIL 162
P+A+ISD GTHFCN+ E+L+KKY ITH+ +T YHPQTSGQ + R ++ IL
Sbjct: 767 PRAMISDGGTHFCNKPFESLMKKYGITHKVATPYHPQTSGQKDSKARLVRWIL 819
>gb|ABG37657.1| integrase [Populus trichocarpa]
Length = 1738
Score = 181 bits (460), Expect = 7e-44
Identities = 79/108 (73%), Positives = 97/108 (89%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+A+ISD GTHFCN+ E+L+KKY ITH+ +T YHPQTSGQ E++NREIK ILEKTVNPN
Sbjct: 868 PRAMISDGGTHFCNKPFESLMKKYGITHKVATPYHPQTSGQVELANREIKQILEKTVNPN 927
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL+DAL AY+TA KT +GMSPYRL++GKPCHLP+E+EH+AYW
Sbjct: 928 RKDWSLRLNDALWAYRTAYKTSLGMSPYRLVYGKPCHLPVEIEHKAYW 975
Score = 71.6 bits (174), Expect = 1e-10
Identities = 31/53 (58%), Positives = 41/53 (77%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSIL 162
P+A+ISD GTHFCN+ E+L+KKY ITH+ +T YHPQTSGQ + R ++ IL
Sbjct: 619 PRAMISDGGTHFCNKPFESLMKKYGITHKVATPYHPQTSGQKDSKARLVRWIL 671
>gb|ABG37656.1| integrase [Populus trichocarpa]
Length = 694
Score = 181 bits (458), Expect = 1e-43
Identities = 80/108 (74%), Positives = 96/108 (88%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+A+ISD GTHFCN+ E L+KKY ITH+ +T YHPQTSGQ E++NREIK ILEKT+NPN
Sbjct: 306 PRAMISDGGTHFCNKSFEFLMKKYGITHKFATPYHPQTSGQVELANREIKQILEKTMNPN 365
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL+DAL AYQTA KT +GMSPYRL++GKPCHLP+ELEH+AYW
Sbjct: 366 RKDWSLRLNDALWAYQTAYKTSLGMSPYRLVYGKPCHLPVELEHKAYW 413
>gb|ABG37671.1| integrase [Populus trichocarpa]
Length = 1332
Score = 179 bits (455), Expect = 3e-43
Identities = 79/108 (73%), Positives = 96/108 (88%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+A+ISD GTHFCN E+L+KKY ITH+ +T YHPQTSGQ E++NREIK ILEKTVNPN
Sbjct: 955 PRAMISDGGTHFCNTSFESLMKKYGITHKVATPYHPQTSGQIELANREIKQILEKTVNPN 1014
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL+DAL AY+TA KT +GMSPY+L++GKPCHLP+ELEH+AYW
Sbjct: 1015 RKDWSLRLNDALWAYRTAYKTSLGMSPYKLVYGKPCHLPVELEHKAYW 1062
>gb|ABG37670.1| integrase [Populus trichocarpa]
Length = 649
Score = 176 bits (445), Expect = 4e-42
Identities = 76/108 (70%), Positives = 95/108 (87%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+A+ISD GTHFCN+ E+L+KKY ITH+ +T YHPQTSGQ E++N+EIK ILEKTVNPN
Sbjct: 267 PRAMISDGGTHFCNKPFESLMKKYGITHKVATPYHPQTSGQVELANKEIKQILEKTVNPN 326
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
KDWS+RL+DAL AY+T KT +GMSPYRL++GKPCHLP+E+EH+AYW
Sbjct: 327 WKDWSLRLNDALWAYRTTYKTSLGMSPYRLVYGKPCHLPVEIEHKAYW 374
>gb|ABD63142.1| Retrotransposon gag protein [Asparagus officinalis]
Length = 1788
Score = 174 bits (440), Expect = 2e-41
Identities = 80/108 (74%), Positives = 93/108 (86%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+AIISD GTHFCNR EAL++KY+ITH+ ST YHPQTSGQ EV+NR+IK ILEKTVN N
Sbjct: 1482 PRAIISDNGTHFCNRSFEALMRKYSITHKLSTPYHPQTSGQVEVTNRQIKQILEKTVNHN 1541
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS++L DAL AY+TA K +GMSPYRL+FGK CHLP+ELEHRA W
Sbjct: 1542 RKDWSVKLCDALWAYRTAFKANLGMSPYRLVFGKACHLPVELEHRAMW 1589
>gb|AHN65359.1| integrase [Glycine max]
Length = 316
Score = 173 bits (439), Expect = 2e-41
Identities = 77/108 (71%), Positives = 92/108 (85%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAI+SD+GTHFCNR + ALLKKY + HR ST YHPQT+GQAE+SNREIK ILEK V P+
Sbjct: 71 PKAIVSDQGTHFCNRTMHALLKKYGVVHRVSTPYHPQTNGQAEISNREIKRILEKIVQPS 130
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS RLDDAL A++ A K IGMSPYR++FGK CHLP+++EH+AYW
Sbjct: 131 RKDWSTRLDDALWAHRAAYKAPIGMSPYRVVFGKACHLPVKIEHKAYW 178
>ref|XP_006471184.1| PREDICTED: uncharacterized protein LOC102621637 [Citrus sinensis]
Length = 340
Score = 167 bits (422), Expect = 2e-39
Identities = 72/109 (66%), Positives = 91/109 (83%)
Frame = +1
Query: 1 TPKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNP 180
TP+AIISD GTHFCN++ A + KY + H+ +TAYHPQ++GQAEVSNREIK ILEK VNP
Sbjct: 99 TPRAIISDEGTHFCNKIFAAAMMKYGVRHKIATAYHPQSNGQAEVSNREIKKILEKVVNP 158
Query: 181 NRKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+ L D+L AY+T KT +GMSPYR+++GK CHLP+ELEH+A+W
Sbjct: 159 TRKDWSLHLHDSLWAYRTTYKTQLGMSPYRIVYGKACHLPLELEHKAHW 207
>ref|XP_004515949.1| PREDICTED: uncharacterized protein LOC101504796 [Cicer arietinum]
Length = 1621
Score = 166 bits (419), Expect = 4e-39
Identities = 74/108 (68%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+A+ISD GTHF NR +E LLKKYN+ HR +T YHPQTSGQ EV NR++K ILEKTV +
Sbjct: 826 PRALISDEGTHFLNRNMEYLLKKYNVRHRVATPYHPQTSGQVEVPNRQLKRILEKTVTAS 885
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS +LDDAL AY+TA KT +GMSPY++++GK CHLP+ELEHRA+W
Sbjct: 886 RKDWSTKLDDALWAYRTAFKTSLGMSPYQIVYGKACHLPLELEHRAFW 933
>ref|XP_006575971.1| PREDICTED: uncharacterized protein LOC102659921, partial [Glycine
max]
Length = 318
Score = 165 bits (418), Expect = 5e-39
Identities = 76/107 (71%), Positives = 91/107 (85%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+AI+SD+GTHFCNR + ALLKKY + H+ ST YHPQT+GQAE+SNREIK ILEK V PN
Sbjct: 212 PRAIVSDQGTHFCNRSMYALLKKYGVMHKISTPYHPQTNGQAEISNREIKRILEKIVQPN 271
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAY 324
KDWS RLDDAL A++TA K LIGMSPYR++FGK CHL +E+EH+AY
Sbjct: 272 IKDWSTRLDDALWAHKTAYKALIGMSPYRVVFGKACHLLLEIEHKAY 318
>emb|CAN78799.1| hypothetical protein VITISV_038948 [Vitis vinifera]
Length = 1676
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1436 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1495
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1496 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1543
>emb|CAN81490.1| hypothetical protein VITISV_031325 [Vitis vinifera]
Length = 2142
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1650 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1709
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1710 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1757
>emb|CAN79155.1| hypothetical protein VITISV_025696 [Vitis vinifera]
Length = 2196
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1432 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1491
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1492 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1539
>emb|CAN81739.1| hypothetical protein VITISV_009673 [Vitis vinifera]
Length = 2377
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 89/108 (82%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
P+AIISD G+HFCN+ LL+KY + H+ ST YHPQT+GQAE++NREIK IL K VN
Sbjct: 1508 PRAIISDGGSHFCNKPFSTLLQKYGVRHKVSTPYHPQTNGQAELANREIKRILTKVVNTT 1567
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS +L DAL AY+TA KT++GMSPYR+++GK CHLP+ELEHRAYW
Sbjct: 1568 RKDWSTKLSDALWAYRTAYKTVLGMSPYRIVYGKACHLPVELEHRAYW 1615
>emb|CAN65053.1| hypothetical protein VITISV_033133 [Vitis vinifera]
Length = 1897
Score = 163 bits (413), Expect = 2e-38
Identities = 74/108 (68%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD GTHFCN+ EALL KY + H+ +T YHPQTSGQ E+SNREIK+IL K VN N
Sbjct: 1499 PKAIISDGGTHFCNKPFEALLAKYGVKHKVATPYHPQTSGQVELSNREIKNILMKVVNTN 1558
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS++L D+L AY+TA KT++GMSPYRL++GK CHLPIE+E +A+W
Sbjct: 1559 RKDWSVKLLDSLWAYRTAYKTILGMSPYRLVYGKACHLPIEIEFKAWW 1606
>emb|CAN73555.1| hypothetical protein VITISV_021948 [Vitis vinifera]
Length = 1600
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1455 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1514
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1515 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1562
>emb|CAN76883.1| hypothetical protein VITISV_036351 [Vitis vinifera]
Length = 2618
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1689 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1748
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1749 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1796
>emb|CAN65863.1| hypothetical protein VITISV_015140 [Vitis vinifera]
Length = 1918
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1638 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1697
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1698 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1745
>emb|CAN62600.1| hypothetical protein VITISV_027792 [Vitis vinifera]
Length = 1707
Score = 163 bits (413), Expect = 2e-38
Identities = 72/108 (66%), Positives = 91/108 (84%)
Frame = +1
Query: 4 PKAIISDRGTHFCNRVVEALLKKYNITHRTSTAYHPQTSGQAEVSNREIKSILEKTVNPN 183
PKAIISD G HFCN+ EALL KY + H+ +T YHPQTSGQ E++NREIK+IL K VN N
Sbjct: 1467 PKAIISDGGAHFCNKPFEALLSKYGVKHKVATPYHPQTSGQVELANREIKNILMKVVNSN 1526
Query: 184 RKDWSMRLDDAL*AYQTA*KTLIGMSPYRLIFGKPCHLPIELEHRAYW 327
RKDWS+RL D+L AY+TA KT++GMSPYRL++GK CHLP+E+E++A+W
Sbjct: 1527 RKDWSIRLHDSLWAYRTAYKTILGMSPYRLVYGKACHLPVEVEYKAWW 1574