BLASTX nr result
ID: Cocculus23_contig00020401
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Cocculus23_contig00020401
(956 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EXB49969.1| Microtubule-associated protein RP/EB family membe... 420 e-115
ref|XP_002276213.1| PREDICTED: microtubule-associated protein RP... 413 e-113
ref|XP_006439614.1| hypothetical protein CICLE_v10021657mg [Citr... 412 e-112
ref|XP_006376816.1| hypothetical protein POPTR_0012s07120g [Popu... 412 e-112
ref|XP_007209454.1| hypothetical protein PRUPE_ppa009753mg [Prun... 411 e-112
ref|XP_006476627.1| PREDICTED: microtubule-associated protein RP... 410 e-112
ref|XP_006439615.1| hypothetical protein CICLE_v10021657mg [Citr... 410 e-112
gb|ABK93021.1| unknown [Populus trichocarpa] 410 e-112
ref|XP_006476626.1| PREDICTED: microtubule-associated protein RP... 409 e-111
ref|XP_002318604.2| microtubule-associated EB1 family protein [P... 407 e-111
ref|XP_007037296.1| Microtubule end binding protein EB1A [Theobr... 407 e-111
ref|XP_004299378.1| PREDICTED: microtubule-associated protein RP... 401 e-109
ref|XP_006374473.1| hypothetical protein POPTR_0015s07430g [Popu... 399 e-109
ref|XP_004236000.1| PREDICTED: microtubule-associated protein RP... 394 e-107
ref|XP_006364190.1| PREDICTED: microtubule-associated protein RP... 394 e-107
dbj|BAH58381.1| AtEB1b-like protein [Nicotiana tabacum] 393 e-107
ref|XP_004137403.1| PREDICTED: microtubule-associated protein RP... 392 e-107
ref|XP_006374472.1| microtubule-associated EB1 family protein [P... 391 e-106
ref|XP_006578579.1| PREDICTED: microtubule-associated protein RP... 390 e-106
gb|EYU35848.1| hypothetical protein MIMGU_mgv1a024615mg [Mimulus... 388 e-105
>gb|EXB49969.1| Microtubule-associated protein RP/EB family member 3 [Morus
notabilis]
Length = 277
Score = 420 bits (1079), Expect = e-115
Identities = 214/270 (79%), Positives = 230/270 (85%), Gaps = 9/270 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQM+DMT+PG VPMHKV
Sbjct: 1 MATNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMIDMTYPGAVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKIDKHIEVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIDKHIEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG---------SGPKQGKMCSM 405
IMNENYNPVERR KGGKERN KG K +KSLQ NN N G SGPKQGK S
Sbjct: 121 IMNENYNPVERRGKGGKERNFKGSQKNSKSLQTNNTHNAGSGDAAGLRNSGPKQGKTYSA 180
Query: 404 SGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIK 225
SG DS SEIQALS +I+DLKLSVD LEKERDFYFAKLRDIEILCQ PELE+LP+A A+K
Sbjct: 181 SGAADSTSEIQALSNEITDLKLSVDVLEKERDFYFAKLRDIEILCQDPELENLPMAVAVK 240
Query: 224 KILYAVDSKESALTEAQELVTTSVNSSSIE 135
KILYAVD+KESAL+EAQE + S N+S E
Sbjct: 241 KILYAVDAKESALSEAQEYIQQSANASKEE 270
>ref|XP_002276213.1| PREDICTED: microtubule-associated protein RP/EB family member 3
[Vitis vinifera] gi|296086393|emb|CBI31982.3| unnamed
protein product [Vitis vinifera]
Length = 280
Score = 413 bits (1062), Expect = e-113
Identities = 207/263 (78%), Positives = 228/263 (86%), Gaps = 9/263 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MAA+IGMMD AYFVGR EIL+WINATL LNLS+VEEAASGAVQCQMMDM HPGVVPMHKV
Sbjct: 1 MAASIGMMDAAYFVGRGEILSWINATLHLNLSKVEEAASGAVQCQMMDMIHPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYK+LQEVFNKLKI+KHIEVNKLVKGRPLDNLEFLQWLKRYCDS+NGG
Sbjct: 61 NFDAKTEYDMIQNYKILQEVFNKLKIEKHIEVNKLVKGRPLDNLEFLQWLKRYCDSMNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNL---------GSGPKQGKMCSM 405
IMNENYNPVERRCKGGKERN KG +K +KSLQANN++N+ SGPK M
Sbjct: 121 IMNENYNPVERRCKGGKERNIKGSYKSSKSLQANNLSNVSDNGICIDESSGPKGRHKSEM 180
Query: 404 SGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIK 225
SGG +S EIQ L ++I+DLKLSVD LEKERDFYF KLRDIEILCQTPE+E LP++ AIK
Sbjct: 181 SGGANSVGEIQGLCQEIADLKLSVDDLEKERDFYFGKLRDIEILCQTPEVEDLPMSMAIK 240
Query: 224 KILYAVDSKESALTEAQELVTTS 156
KILYAVD+K SAL EAQE++T S
Sbjct: 241 KILYAVDAKVSALGEAQEIITKS 263
>ref|XP_006439614.1| hypothetical protein CICLE_v10021657mg [Citrus clementina]
gi|557541876|gb|ESR52854.1| hypothetical protein
CICLE_v10021657mg [Citrus clementina]
Length = 266
Score = 412 bits (1058), Expect = e-112
Identities = 206/262 (78%), Positives = 232/262 (88%), Gaps = 5/262 (1%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA NIGMMD AYFVGR+E+L WIN LQL+LSR+EEAASGAVQCQM+DMT+PGVVPMHKV
Sbjct: 1 MATNIGMMDSAYFVGRNELLTWINNRLQLHLSRIEEAASGAVQCQMLDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLGSG-----PKQGKMCSMSGGD 393
IMNENYNPVERRCKGGKER+S+G K++KSLQ NN+ N GSG P+Q K+ G
Sbjct: 121 IMNENYNPVERRCKGGKERSSRGSQKISKSLQTNNMHNAGSGDIGHRPRQAKIY----GA 176
Query: 392 DSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKILY 213
+SA E+QALS++I+DLKLSVD LEKERDFYFAKLRDIEILCQTPELE+LPVA AIKKILY
Sbjct: 177 NSAQEVQALSKEITDLKLSVDLLEKERDFYFAKLRDIEILCQTPELENLPVAVAIKKILY 236
Query: 212 AVDSKESALTEAQELVTTSVNS 147
A D+KESAL EAQE ++ ++ +
Sbjct: 237 AADAKESALAEAQEYLSQTLEA 258
>ref|XP_006376816.1| hypothetical protein POPTR_0012s07120g [Populus trichocarpa]
gi|550326558|gb|ERP54613.1| hypothetical protein
POPTR_0012s07120g [Populus trichocarpa]
Length = 276
Score = 412 bits (1058), Expect = e-112
Identities = 205/275 (74%), Positives = 235/275 (85%), Gaps = 10/275 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKI+KHIEVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIEKHIEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG----------SGPKQGKMCS 408
IMNENYNPVERR KGGK+RNSKG K KSLQANN+ N SGPKQG+ +
Sbjct: 121 IMNENYNPVERRSKGGKDRNSKGSQKTTKSLQANNLHNSASGDTVDLNKMSGPKQGRGSA 180
Query: 407 MSGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAI 228
++GG + EIQALS+++++LKLSVDHLEKERDFYFAKLRDIEILCQ PE+E LP+ AI
Sbjct: 181 VAGGATYSEEIQALSKEVTNLKLSVDHLEKERDFYFAKLRDIEILCQIPEMEDLPMTVAI 240
Query: 227 KKILYAVDSKESALTEAQELVTTSVNSSSIEMVND 123
KKILYA D+KESAL EAQE ++ ++N+ E+ ++
Sbjct: 241 KKILYADDAKESALEEAQEYLSEAINTVETEVESE 275
>ref|XP_007209454.1| hypothetical protein PRUPE_ppa009753mg [Prunus persica]
gi|462405189|gb|EMJ10653.1| hypothetical protein
PRUPE_ppa009753mg [Prunus persica]
Length = 279
Score = 411 bits (1056), Expect = e-112
Identities = 207/271 (76%), Positives = 231/271 (85%), Gaps = 10/271 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIG+MD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGIMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQEVFNKLKI+KH+EVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQEVFNKLKIEKHLEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG----------SGPKQGKMCS 408
IMNENYNPVERRCKGGK+R KG K +KSLQ NN+ N G SGPKQGK +
Sbjct: 121 IMNENYNPVERRCKGGKDRYPKGSQKFSKSLQTNNLHNPGSGDTVGLSKTSGPKQGKPYA 180
Query: 407 MSGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAI 228
+GG +S+ E+QALS++I+DLKLSVD LEKERDFYFAKLRDIEILCQT ELE LP+A AI
Sbjct: 181 AAGGANSSGEVQALSKEITDLKLSVDLLEKERDFYFAKLRDIEILCQTSELEELPMAVAI 240
Query: 227 KKILYAVDSKESALTEAQELVTTSVNSSSIE 135
KKILYA D+KES L EAQE + S+N+ E
Sbjct: 241 KKILYAADAKESPLAEAQEYLYQSMNADEKE 271
>ref|XP_006476627.1| PREDICTED: microtubule-associated protein RP/EB family member
1B-like isoform X2 [Citrus sinensis]
Length = 266
Score = 410 bits (1054), Expect = e-112
Identities = 205/262 (78%), Positives = 232/262 (88%), Gaps = 5/262 (1%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA NIGMMD AYFVGR+E+L WIN LQL+LSR+EEAASGAVQCQM+DMT+PGVVPMHKV
Sbjct: 1 MATNIGMMDSAYFVGRNELLTWINNRLQLHLSRIEEAASGAVQCQMLDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKI+KHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIEKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLGSG-----PKQGKMCSMSGGD 393
IMNENYNPVERRCKGGKER+S+G K++KSLQ NN+ N GSG P+Q K+ G
Sbjct: 121 IMNENYNPVERRCKGGKERSSRGSQKISKSLQTNNMHNAGSGDIGHRPRQAKIY----GA 176
Query: 392 DSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKILY 213
+SA E+QALS++I+DLKLSVD LEKERDFYFAKLRDIEILCQTPELE+LPVA AIKKILY
Sbjct: 177 NSAQEVQALSKEITDLKLSVDLLEKERDFYFAKLRDIEILCQTPELENLPVAVAIKKILY 236
Query: 212 AVDSKESALTEAQELVTTSVNS 147
A D+KESAL EAQE ++ ++ +
Sbjct: 237 AADAKESALAEAQEYLSQTLEA 258
>ref|XP_006439615.1| hypothetical protein CICLE_v10021657mg [Citrus clementina]
gi|557541877|gb|ESR52855.1| hypothetical protein
CICLE_v10021657mg [Citrus clementina]
Length = 270
Score = 410 bits (1054), Expect = e-112
Identities = 206/266 (77%), Positives = 232/266 (87%), Gaps = 9/266 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA NIGMMD AYFVGR+E+L WIN LQL+LSR+EEAASGAVQCQM+DMT+PGVVPMHKV
Sbjct: 1 MATNIGMMDSAYFVGRNELLTWINNRLQLHLSRIEEAASGAVQCQMLDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG---------SGPKQGKMCSM 405
IMNENYNPVERRCKGGKER+S+G K++KSLQ NN+ N G SGP+Q K+
Sbjct: 121 IMNENYNPVERRCKGGKERSSRGSQKISKSLQTNNMHNAGSGDIGHSKISGPRQAKIY-- 178
Query: 404 SGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIK 225
G +SA E+QALS++I+DLKLSVD LEKERDFYFAKLRDIEILCQTPELE+LPVA AIK
Sbjct: 179 --GANSAQEVQALSKEITDLKLSVDLLEKERDFYFAKLRDIEILCQTPELENLPVAVAIK 236
Query: 224 KILYAVDSKESALTEAQELVTTSVNS 147
KILYA D+KESAL EAQE ++ ++ +
Sbjct: 237 KILYAADAKESALAEAQEYLSQTLEA 262
>gb|ABK93021.1| unknown [Populus trichocarpa]
Length = 276
Score = 410 bits (1054), Expect = e-112
Identities = 204/275 (74%), Positives = 235/275 (85%), Gaps = 10/275 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNY+VLQ+VFNKLKI+KHIEVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYEVLQDVFNKLKIEKHIEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG----------SGPKQGKMCS 408
IMNENYNPVERR KGGK+RNSKG K KSLQANN+ N SGPKQG+ +
Sbjct: 121 IMNENYNPVERRSKGGKDRNSKGSQKTTKSLQANNLHNSASGDTVDLNKMSGPKQGRGSA 180
Query: 407 MSGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAI 228
++GG + EIQALS+++++LKLSVDHLEKERDFYFAKLRDIEILCQ PE+E LP+ AI
Sbjct: 181 VAGGATYSEEIQALSKEVTNLKLSVDHLEKERDFYFAKLRDIEILCQIPEMEDLPMTVAI 240
Query: 227 KKILYAVDSKESALTEAQELVTTSVNSSSIEMVND 123
KKILYA D+KESAL EAQE ++ ++N+ E+ ++
Sbjct: 241 KKILYADDAKESALEEAQEYLSEAINTVETEVESE 275
>ref|XP_006476626.1| PREDICTED: microtubule-associated protein RP/EB family member
1B-like isoform X1 [Citrus sinensis]
Length = 270
Score = 409 bits (1050), Expect = e-111
Identities = 205/266 (77%), Positives = 232/266 (87%), Gaps = 9/266 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA NIGMMD AYFVGR+E+L WIN LQL+LSR+EEAASGAVQCQM+DMT+PGVVPMHKV
Sbjct: 1 MATNIGMMDSAYFVGRNELLTWINNRLQLHLSRIEEAASGAVQCQMLDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKI+KHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIEKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG---------SGPKQGKMCSM 405
IMNENYNPVERRCKGGKER+S+G K++KSLQ NN+ N G SGP+Q K+
Sbjct: 121 IMNENYNPVERRCKGGKERSSRGSQKISKSLQTNNMHNAGSGDIGHSKISGPRQAKIY-- 178
Query: 404 SGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIK 225
G +SA E+QALS++I+DLKLSVD LEKERDFYFAKLRDIEILCQTPELE+LPVA AIK
Sbjct: 179 --GANSAQEVQALSKEITDLKLSVDLLEKERDFYFAKLRDIEILCQTPELENLPVAVAIK 236
Query: 224 KILYAVDSKESALTEAQELVTTSVNS 147
KILYA D+KESAL EAQE ++ ++ +
Sbjct: 237 KILYAADAKESALAEAQEYLSQTLEA 262
>ref|XP_002318604.2| microtubule-associated EB1 family protein [Populus trichocarpa]
gi|550326557|gb|EEE96824.2| microtubule-associated EB1
family protein [Populus trichocarpa]
Length = 272
Score = 407 bits (1046), Expect = e-111
Identities = 203/271 (74%), Positives = 232/271 (85%), Gaps = 6/271 (2%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKI+KHIEVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIEKHIEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLGSG------PKQGKMCSMSGG 396
IMNENYNPVERR KGGK+RNSKG K KSLQANN+ N SG GK +++GG
Sbjct: 121 IMNENYNPVERRSKGGKDRNSKGSQKTTKSLQANNLHNSASGDTVDLNKMSGKGSAVAGG 180
Query: 395 DDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKIL 216
+ EIQALS+++++LKLSVDHLEKERDFYFAKLRDIEILCQ PE+E LP+ AIKKIL
Sbjct: 181 ATYSEEIQALSKEVTNLKLSVDHLEKERDFYFAKLRDIEILCQIPEMEDLPMTVAIKKIL 240
Query: 215 YAVDSKESALTEAQELVTTSVNSSSIEMVND 123
YA D+KESAL EAQE ++ ++N+ E+ ++
Sbjct: 241 YADDAKESALEEAQEYLSEAINTVETEVESE 271
>ref|XP_007037296.1| Microtubule end binding protein EB1A [Theobroma cacao]
gi|508774541|gb|EOY21797.1| Microtubule end binding
protein EB1A [Theobroma cacao]
Length = 268
Score = 407 bits (1046), Expect = e-111
Identities = 208/271 (76%), Positives = 231/271 (85%), Gaps = 10/271 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQM+DMT+PGVVPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMIDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQEVFNKLKI+KHIEVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQEVFNKLKIEKHIEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLGSG----------PKQGKMCS 408
IMNENYNPVERR KGGKERNSKG HK +KSLQANN+ N SG KQGK
Sbjct: 121 IMNENYNPVERRVKGGKERNSKGSHKSSKSLQANNLHNSASGDIAGINKNSVSKQGK--- 177
Query: 407 MSGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAI 228
+G ++ ++QALS++I+DLKLSVD LEKERDFYFAKLRDIEILCQTPE+E+LP+A AI
Sbjct: 178 -TGVAATSGDVQALSKEIADLKLSVDLLEKERDFYFAKLRDIEILCQTPEVENLPMAVAI 236
Query: 227 KKILYAVDSKESALTEAQELVTTSVNSSSIE 135
KKILYA D+KESAL EAQE ++ S S E
Sbjct: 237 KKILYAADAKESALEEAQEYISRSAESGETE 267
>ref|XP_004299378.1| PREDICTED: microtubule-associated protein RP/EB family member
1A-like [Fragaria vesca subsp. vesca]
Length = 277
Score = 401 bits (1030), Expect = e-109
Identities = 200/266 (75%), Positives = 229/266 (86%), Gaps = 5/266 (1%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIG+MD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGIMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYK+LQEVFNKLKI+K +EVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKILQEVFNKLKIEKPLEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNV-----TNLGSGPKQGKMCSMSGGD 393
IMNENYNPVERRCKGGKER+ KG K++KSLQANN SGPKQGK +++GG
Sbjct: 121 IMNENYNPVERRCKGGKERHPKGSQKISKSLQANNSGDPVGLRRTSGPKQGKTSAVAGGT 180
Query: 392 DSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKILY 213
A E+QAL+++I+DLKLSVD LEKERDFYF KLRDIEILCQT ELE +P++ AIKKILY
Sbjct: 181 YYAGEVQALTKEITDLKLSVDLLEKERDFYFGKLRDIEILCQTSELESVPMSVAIKKILY 240
Query: 212 AVDSKESALTEAQELVTTSVNSSSIE 135
A D+KESALT+AQE + + ++ E
Sbjct: 241 AADAKESALTKAQEYLVSVGSAEDAE 266
>ref|XP_006374473.1| hypothetical protein POPTR_0015s07430g [Populus trichocarpa]
gi|550322237|gb|ERP52270.1| hypothetical protein
POPTR_0015s07430g [Populus trichocarpa]
Length = 276
Score = 399 bits (1025), Expect = e-109
Identities = 198/276 (71%), Positives = 234/276 (84%), Gaps = 10/276 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYK+LQ++FNKLKI+KH+EVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKILQDIFNKLKIEKHLEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG----------SGPKQGKMCS 408
IMNENYNP+ERR KGG++RNS+G K KS QA+N+ N SG KQG+ +
Sbjct: 121 IMNENYNPLERRSKGGRDRNSRGSQKTTKSQQASNMHNSSSGYTVDLNKMSGQKQGRGSA 180
Query: 407 MSGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAI 228
+ G +S+ EIQALS++I+D+KLSVD LEKERDFYFAKLRDIEILCQTPELE LPV AI
Sbjct: 181 VVGEANSSEEIQALSKEIADMKLSVDLLEKERDFYFAKLRDIEILCQTPELEDLPVVVAI 240
Query: 227 KKILYAVDSKESALTEAQELVTTSVNSSSIEMVNDL 120
KKILYA D+KESAL EAQE ++ ++ + + E+ +++
Sbjct: 241 KKILYAADTKESALEEAQEYLSEAICTGATEVESEV 276
>ref|XP_004236000.1| PREDICTED: microtubule-associated protein RP/EB family member
1B-like [Solanum lycopersicum]
Length = 267
Score = 394 bits (1013), Expect = e-107
Identities = 195/264 (73%), Positives = 228/264 (86%), Gaps = 8/264 (3%)
Frame = -1
Query: 911 ANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKVNF 732
ANIG+MD AYFVGR+E+L WINA LQLNL+R+EE ASGAVQCQMMDMT+PG VPMHKVNF
Sbjct: 2 ANIGIMDSAYFVGRNELLTWINARLQLNLTRIEEVASGAVQCQMMDMTYPGAVPMHKVNF 61
Query: 731 DAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGGIM 552
DAKTEYDMIQNYKVLQ+VF+KLKIDKHIEVN+LVKGRPLDNLEFLQWLKRYC+SVNGGIM
Sbjct: 62 DAKTEYDMIQNYKVLQDVFSKLKIDKHIEVNRLVKGRPLDNLEFLQWLKRYCESVNGGIM 121
Query: 551 NENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG--------SGPKQGKMCSMSGG 396
NENYNP+ERR K G+ERN KG + AKSL NN N G +G KQG+ + GG
Sbjct: 122 NENYNPLERRSKVGRERNVKGSQRSAKSLLTNNSHNPGLGEGLTKTTGIKQGRSSPVMGG 181
Query: 395 DDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKIL 216
+S++EIQALS++++DLKLSVDHLEKERDFYFAKLRDIEILCQTP+LE +P+A A+KKIL
Sbjct: 182 VNSSTEIQALSKEVTDLKLSVDHLEKERDFYFAKLRDIEILCQTPDLEDIPMAMAVKKIL 241
Query: 215 YAVDSKESALTEAQELVTTSVNSS 144
YA D++ESAL EAQE+++ SV+ S
Sbjct: 242 YAADARESALAEAQEVLSHSVDGS 265
>ref|XP_006364190.1| PREDICTED: microtubule-associated protein RP/EB family member
1B-like [Solanum tuberosum]
Length = 267
Score = 394 bits (1011), Expect = e-107
Identities = 195/264 (73%), Positives = 227/264 (85%), Gaps = 8/264 (3%)
Frame = -1
Query: 911 ANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKVNF 732
ANIG+MD AYFVGR+E+L WINA LQLNL+R+EE ASGAVQCQMMDMT+PG VPMHKVNF
Sbjct: 2 ANIGIMDSAYFVGRNELLTWINARLQLNLTRIEEVASGAVQCQMMDMTYPGAVPMHKVNF 61
Query: 731 DAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGGIM 552
DAKTEYDMIQNYKVLQ+VF+KLKIDKHIEVN+LVKGRPLDNLEFLQWLKRYC+SVNGGIM
Sbjct: 62 DAKTEYDMIQNYKVLQDVFSKLKIDKHIEVNRLVKGRPLDNLEFLQWLKRYCESVNGGIM 121
Query: 551 NENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG--------SGPKQGKMCSMSGG 396
NENYNP+ERR K G+ERN KG + AKSL NN N G G KQG+ + GG
Sbjct: 122 NENYNPLERRSKVGRERNVKGSQRSAKSLLTNNSHNPGLGEGMTKIKGIKQGRSSPVMGG 181
Query: 395 DDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKIL 216
+S++EIQALS++++DLKLSVDHLEKERDFYFAKLRDIEILCQTP+LE +P+A A+KKIL
Sbjct: 182 VNSSTEIQALSKEVTDLKLSVDHLEKERDFYFAKLRDIEILCQTPDLEDVPMAMAVKKIL 241
Query: 215 YAVDSKESALTEAQELVTTSVNSS 144
YA D++ESAL EAQE+++ SV+ S
Sbjct: 242 YAADARESALAEAQEVLSHSVDGS 265
>dbj|BAH58381.1| AtEB1b-like protein [Nicotiana tabacum]
Length = 267
Score = 393 bits (1009), Expect = e-107
Identities = 195/264 (73%), Positives = 227/264 (85%), Gaps = 8/264 (3%)
Frame = -1
Query: 911 ANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKVNF 732
ANIG+MD AYFVGR+E+L+WINA L LNL+R+EEAASGAVQCQMMDMT+PG VPMHKVNF
Sbjct: 2 ANIGIMDSAYFVGRNELLSWINARLHLNLTRIEEAASGAVQCQMMDMTYPGAVPMHKVNF 61
Query: 731 DAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGGIM 552
DAKTEYDMIQNYKVLQ+VFNKLKIDKHIEVN+LVKGRPLDNLEFLQWLKRYC+SVNGGIM
Sbjct: 62 DAKTEYDMIQNYKVLQDVFNKLKIDKHIEVNRLVKGRPLDNLEFLQWLKRYCESVNGGIM 121
Query: 551 NENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLG--------SGPKQGKMCSMSGG 396
NENYN ++RR K GKERN KG + AKSLQ NN N G +G KQG+ + G
Sbjct: 122 NENYNALDRRSKVGKERNVKGSQRYAKSLQTNNCHNPGLGEDITRITGIKQGRSSPVMGV 181
Query: 395 DDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKIL 216
+S++EIQALS++++DLKLSVDHLEKERDFYFAKLRDIEILCQTPEL+++P+A A+KKIL
Sbjct: 182 VNSSAEIQALSKEVTDLKLSVDHLEKERDFYFAKLRDIEILCQTPELDNVPMAVAVKKIL 241
Query: 215 YAVDSKESALTEAQELVTTSVNSS 144
YA D++ESAL EAQE + SV+ S
Sbjct: 242 YAADARESALAEAQEFPSESVDGS 265
>ref|XP_004137403.1| PREDICTED: microtubule-associated protein RP/EB family member
1B-like [Cucumis sativus]
gi|449531603|ref|XP_004172775.1| PREDICTED:
microtubule-associated protein RP/EB family member
1B-like [Cucumis sativus]
Length = 272
Score = 392 bits (1008), Expect = e-107
Identities = 202/272 (74%), Positives = 228/272 (83%), Gaps = 7/272 (2%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQM+DMT+PG VPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNCLQLNLSRIEEAASGAVQCQMIDMTYPGAVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKI+KHIEVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIEKHIEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGK-ERNSKGVHKVAKSLQANNVTNLGSG------PKQGKMCSMSG 399
IMNENYNPVERRCKGGK KG K AKSLQ +N+ + GSG K GK ++G
Sbjct: 121 IMNENYNPVERRCKGGKGGATMKGCQKAAKSLQTHNMHSHGSGDSVEPRSKPGKN-GVTG 179
Query: 398 GDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKI 219
G +S++EIQALS++I++LK+SVD LEKERDFYFAKLRDIEI CQ ELE LP+ AAIK I
Sbjct: 180 GTNSSTEIQALSKEITELKMSVDLLEKERDFYFAKLRDIEIFCQMSELEDLPMTAAIKMI 239
Query: 218 LYAVDSKESALTEAQELVTTSVNSSSIEMVND 123
LYA D+KESALTEAQE + S N S +E N+
Sbjct: 240 LYAADAKESALTEAQEYIYQSQNRSDVENENE 271
>ref|XP_006374472.1| microtubule-associated EB1 family protein [Populus trichocarpa]
gi|550322236|gb|ERP52269.1| microtubule-associated EB1
family protein [Populus trichocarpa]
Length = 274
Score = 391 bits (1005), Expect = e-106
Identities = 196/274 (71%), Positives = 232/274 (84%), Gaps = 8/274 (2%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA+NIGMMD AYFVGR+EIL WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MASNIGMMDSAYFVGRNEILTWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYK+LQ++FNKLKI+KH+EVN+LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKILQDIFNKLKIEKHLEVNRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLGSG------PKQGKMCSMSGG 396
IMNENYNP+ERR KGG++RNS+G K KS QA+N+ N SG G+ ++ G
Sbjct: 121 IMNENYNPLERRSKGGRDRNSRGSQKTTKSQQASNMHNSSSGYTVDLNKMSGRGSAVVGE 180
Query: 395 DDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLP--VAAAIKK 222
+S+ EIQALS++I+D+KLSVD LEKERDFYFAKLRDIEILCQTPELE LP V AIKK
Sbjct: 181 ANSSEEIQALSKEIADMKLSVDLLEKERDFYFAKLRDIEILCQTPELEDLPVRVVVAIKK 240
Query: 221 ILYAVDSKESALTEAQELVTTSVNSSSIEMVNDL 120
ILYA D+KESAL EAQE ++ ++ + + E+ +++
Sbjct: 241 ILYAADTKESALEEAQEYLSEAICTGATEVESEV 274
>ref|XP_006578579.1| PREDICTED: microtubule-associated protein RP/EB family member
1B-like [Glycine max]
Length = 277
Score = 390 bits (1002), Expect = e-106
Identities = 201/271 (74%), Positives = 228/271 (84%), Gaps = 4/271 (1%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA +IG+MD AYFVGR+EIL+WIN LQLNLSR+EEAASGAVQCQMMDMT+PGVVPMHKV
Sbjct: 1 MATSIGIMDSAYFVGRNEILSWINNRLQLNLSRIEEAASGAVQCQMMDMTYPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYKVLQ+VFNKLKI+KHIEV +LVKGRPLDNLEFLQWLKRYCDSVNGG
Sbjct: 61 NFDAKTEYDMIQNYKVLQDVFNKLKIEKHIEVGRLVKGRPLDNLEFLQWLKRYCDSVNGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTNLGSGPK-QGKMCSMSGGDD--- 390
IMNENYNPVERR KGGK+RN KG+ K +KSLQ N + + GSG + K SGG D
Sbjct: 121 IMNENYNPVERRVKGGKDRNVKGL-KSSKSLQTNTMNHSGSGDSLRSKQLRSSGGADGVN 179
Query: 389 SASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAIKKILYA 210
S++EIQALS+Q+SDLKLSVDHLEKERDFYFAKLRDIEILCQ ELE+ P++ AIKKILYA
Sbjct: 180 SSAEIQALSKQVSDLKLSVDHLEKERDFYFAKLRDIEILCQASELENDPMSLAIKKILYA 239
Query: 209 VDSKESALTEAQELVTTSVNSSSIEMVNDLE 117
D+K SAL EAQE + +N + E + E
Sbjct: 240 ADAKGSALDEAQEYLNEVINGNEDEAEAEAE 270
>gb|EYU35848.1| hypothetical protein MIMGU_mgv1a024615mg [Mimulus guttatus]
Length = 278
Score = 388 bits (996), Expect = e-105
Identities = 203/277 (73%), Positives = 223/277 (80%), Gaps = 10/277 (3%)
Frame = -1
Query: 917 MAANIGMMDGAYFVGRSEILAWINATLQLNLSRVEEAASGAVQCQMMDMTHPGVVPMHKV 738
MA NIGMMDGAYFVGR+EILAWINA L LNLSRVEEAASGAVQCQMMDM HPGVVPMHKV
Sbjct: 1 MATNIGMMDGAYFVGRNEILAWINARLHLNLSRVEEAASGAVQCQMMDMAHPGVVPMHKV 60
Query: 737 NFDAKTEYDMIQNYKVLQEVFNKLKIDKHIEVNKLVKGRPLDNLEFLQWLKRYCDSVNGG 558
NFDAKTEYDMIQNYK+LQEVFNKLKI KHIEVNKLVKGRPLDNLEFLQWLKRYCDS+NGG
Sbjct: 61 NFDAKTEYDMIQNYKILQEVFNKLKITKHIEVNKLVKGRPLDNLEFLQWLKRYCDSINGG 120
Query: 557 IMNENYNPVERRCKGGKERNSKGVHKVAKSLQANNVTN------LGSG----PKQGKMCS 408
M ENYNP ERR KGGKERNSKG K KSLQ NN N +GS KQGK+
Sbjct: 121 TMKENYNPTERRNKGGKERNSKGSQKNFKSLQTNNSLNSVSSDGMGSNKISVTKQGKVGG 180
Query: 407 MSGGDDSASEIQALSEQISDLKLSVDHLEKERDFYFAKLRDIEILCQTPELEHLPVAAAI 228
++ +S+ EIQ L+ +++DLKLSVD LEKERDFYFAKLRDIEIL QTPELE+ P++ AI
Sbjct: 181 VATVANSSVEIQELTGELTDLKLSVDLLEKERDFYFAKLRDIEILSQTPELENFPMSMAI 240
Query: 227 KKILYAVDSKESALTEAQELVTTSVNSSSIEMVNDLE 117
KKILYA + KESAL EAQE+V + E +D E
Sbjct: 241 KKILYAGEEKESALAEAQEIVAQIIKVEKTEYSDDDE 277