BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Cocculus23_contig00018977
         (1063 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281534.2| PREDICTED: inositol-pentakisphosphate 2-kina...   292   1e-76
emb|CBI19737.3| unnamed protein product [Vitis vinifera]              292   1e-76
ref|XP_004291851.1| PREDICTED: inositol-pentakisphosphate 2-kina...   281   4e-73
emb|CAN76214.1| hypothetical protein VITISV_009512 [Vitis vinifera]   281   4e-73
ref|XP_004238270.1| PREDICTED: inositol-pentakisphosphate 2-kina...   280   9e-73
ref|XP_007223293.1| hypothetical protein PRUPE_ppa005582mg [Prun...   277   6e-72
gb|EXC25059.1| Inositol-pentakisphosphate 2-kinase [Morus notabi...   275   2e-71
ref|XP_004160062.1| PREDICTED: inositol-pentakisphosphate 2-kina...   272   2e-70
ref|XP_004152686.1| PREDICTED: inositol-pentakisphosphate 2-kina...   272   2e-70
ref|XP_002306883.1| hypothetical protein POPTR_0005s25180g [Popu...   272   2e-70
ref|XP_006341967.1| PREDICTED: inositol-pentakisphosphate 2-kina...   270   1e-69
ref|XP_006341966.1| PREDICTED: inositol-pentakisphosphate 2-kina...   270   1e-69
tpg|DAA64569.1| TPA_exp: inositol-pentakisphosphate 2-kinase 555...   268   3e-69
tpg|DAA64568.1| TPA_exp: inositol-pentakisphosphate 2-kinase 452...   268   3e-69
tpg|DAA64567.1| TPA_exp: inositol-pentakisphosphate 2-kinase 450...   268   3e-69
ref|XP_002510425.1| Inositol-pentakisphosphate 2-kinase, putativ...   268   3e-69
ref|XP_003523790.1| PREDICTED: inositol-pentakisphosphate 2-kina...   261   3e-67
ref|XP_003528009.1| PREDICTED: inositol-pentakisphosphate 2-kina...   259   1e-66
ref|XP_006473539.1| PREDICTED: inositol-pentakisphosphate 2-kina...   259   2e-66
ref|XP_004500991.1| PREDICTED: inositol-pentakisphosphate 2-kina...   258   4e-66

>ref|XP_002281534.2| PREDICTED: inositol-pentakisphosphate 2-kinase-like [Vitis vinifera]
          Length = 582

 Score =  292 bits (748), Expect = 1e-76
 Identities = 151/276 (54%), Positives = 193/276 (69%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLPFS FIAE N+IK+R TRF MHQ LKLHQ EI+++S+Y+PLDLFSGS ++++ A
Sbjct: 166  PKCGFLPFSRFIAEGNAIKKRVTRFRMHQILKLHQQEIAQISEYEPLDLFSGSKEKMHKA 225

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            + A F  PQN FR                +     V +A      FED++K VI AE G 
Sbjct: 226  VKALFTTPQNNFRVFLNGSLIYGGLGGGTDSTSFMVGEA------FEDVLKCVIQAEVGM 279

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R +         + ++ +L++LLEVQKLD+FDIEGAIHA YD++S+PC VC +L +D   
Sbjct: 280  RVESFLHLVSETVSKSGVLDRLLEVQKLDTFDIEGAIHAYYDIVSEPCTVCRDLGEDIAS 339

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             +Y  LHS+  +ES KIVR+YLIAATAKDCS+MISF    +GNSASPYS++YL ST+Q+F
Sbjct: 340  HRYTSLHSIPSDESLKIVRDYLIAATAKDCSLMISFAPRKDGNSASPYSNVYLASTDQSF 399

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            DYKA FIDLDMKPL KM  +YELDQK++  Y QM K
Sbjct: 400  DYKAYFIDLDMKPLKKMQYYYELDQKIVSCYTQMMK 435


>emb|CBI19737.3| unnamed protein product [Vitis vinifera]
          Length = 469

 Score =  292 bits (748), Expect = 1e-76
 Identities = 151/276 (54%), Positives = 193/276 (69%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLPFS FIAE N+IK+R TRF MHQ LKLHQ EI+++S+Y+PLDLFSGS ++++ A
Sbjct: 182  PKCGFLPFSRFIAEGNAIKKRVTRFRMHQILKLHQQEIAQISEYEPLDLFSGSKEKMHKA 241

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            + A F  PQN FR                +     V +A      FED++K VI AE G 
Sbjct: 242  VKALFTTPQNNFRVFLNGSLIYGGLGGGTDSTSFMVGEA------FEDVLKCVIQAEVGM 295

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R +         + ++ +L++LLEVQKLD+FDIEGAIHA YD++S+PC VC +L +D   
Sbjct: 296  RVESFLHLVSETVSKSGVLDRLLEVQKLDTFDIEGAIHAYYDIVSEPCTVCRDLGEDIAS 355

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             +Y  LHS+  +ES KIVR+YLIAATAKDCS+MISF    +GNSASPYS++YL ST+Q+F
Sbjct: 356  HRYTSLHSIPSDESLKIVRDYLIAATAKDCSLMISFAPRKDGNSASPYSNVYLASTDQSF 415

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            DYKA FIDLDMKPL KM  +YELDQK++  Y QM K
Sbjct: 416  DYKAYFIDLDMKPLKKMQYYYELDQKIVSCYTQMMK 451


>ref|XP_004291851.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like [Fragaria vesca
            subsp. vesca]
          Length = 454

 Score =  281 bits (718), Expect = 4e-73
 Identities = 140/274 (51%), Positives = 188/274 (68%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP SE+I E N+IKRR TRF MHQ LKLH GE+S+ S YDPLDLFSGS DRI+ A
Sbjct: 167  PKCGFLPSSEYIDEGNAIKRRITRFRMHQALKLHDGEVSQYSNYDPLDLFSGSKDRIHKA 226

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   FA PQN FR                E   + +       D+FE  +KDVI    G 
Sbjct: 227  IKDLFATPQNNFRVFVNGSLIFGGLGGGAESTNLVIG------DKFETALKDVIQGGDGS 280

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          ++R+ +++QLLE+QKLD  DI+GAIHA YD+IS+PCMVC+ L  D+  
Sbjct: 281  RTVNFLQLVAETVYRSGVMDQLLEIQKLDYLDIDGAIHAYYDIISEPCMVCTELGKDKAS 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             +Y+ LHSL L+ES KIV+++LI+A+AKDCS+M SFR   +G++ SPY+++YLESTNQ +
Sbjct: 341  NRYSSLHSLPLDESLKIVKQFLISASAKDCSLMFSFRPRKDGSTGSPYNNVYLESTNQFY 400

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQM 240
            DYK  FIDLD+K L K+ ++YELD+K+++ Y +M
Sbjct: 401  DYKVYFIDLDLKSLKKLEEYYELDKKIVRCYNEM 434


>emb|CAN76214.1| hypothetical protein VITISV_009512 [Vitis vinifera]
          Length = 696

 Score =  281 bits (718), Expect = 4e-73
 Identities = 151/284 (53%), Positives = 193/284 (67%), Gaps = 8/284 (2%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGE-------ISRLSKYDPLDLFSGS 903
            PKCGFLPFS FIAE N+IK+R TRF MHQ LKLHQ E       I+++S+Y+PLDLFSGS
Sbjct: 210  PKCGFLPFSRFIAEGNAIKKRVTRFRMHQILKLHQQETCSLTPQIAQISEYEPLDLFSGS 269

Query: 902  LDRINHALTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDV 723
             ++++ A+ A F  PQN FR                +     V +A      FED++K V
Sbjct: 270  KEKMHKAVKALFTTPQNNFRVFLNGSLIYGGLGGGTDSTSFMVGEA------FEDVLKCV 323

Query: 722  IHAEHGKRSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSN 543
            I AE G R +         + ++ +L++LLEVQKLD+FDIEGAIHA YD++S+PC VC +
Sbjct: 324  IQAEVGMRVESFLHLVSETVSKSGVLDRLLEVQKLDTFDIEGAIHAYYDIVSEPCTVCRD 383

Query: 542  LKDDELLRKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYL 363
            L +D    +Y  LHS+  +ES KIVR+YLIAATAKDCS+MISF    +GNSASPYS++YL
Sbjct: 384  LGEDIASHRYTSLHSIPSDESLKIVRDYLIAATAKDCSLMISFAPRKDGNSASPYSNVYL 443

Query: 362  EST-NQNFDYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
             ST +Q+FDYKA FIDLDMKPL KM  +YELDQK++  Y QM K
Sbjct: 444  ASTFDQSFDYKAYFIDLDMKPLKKMQYYYELDQKIVSCYTQMMK 487


>ref|XP_004238270.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like [Solanum
            lycopersicum]
          Length = 381

 Score =  280 bits (715), Expect = 9e-73
 Identities = 152/279 (54%), Positives = 188/279 (67%), Gaps = 1/279 (0%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP SEFIA  NSIKR  TRF MHQ LKLHQG+IS +S YDPLDLFSGS DR++ A
Sbjct: 102  PKCGFLPLSEFIASENSIKRTVTRFKMHQALKLHQGKISEISAYDPLDLFSGSSDRVHKA 161

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F  PQN FR                +     V +       FE+ +K VI A  G+
Sbjct: 162  IKGLFETPQNNFRVFLNGSLILGGLGGNADATSCEVGET------FENALKCVIQAVDGQ 215

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLK-DDEL 525
            R+Q         IF + +LN++LEVQKLD+ DIEGAIHA Y+VISQPC VC+ L  +D+L
Sbjct: 216  RTQCFLDLISKTIFSSGLLNKVLEVQKLDNADIEGAIHAYYNVISQPCAVCNKLSAEDQL 275

Query: 524  LRKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQN 345
              +Y+ LHS+S +ES KIVR YLIAATAKD S+MISFR   +G+  SPYS + LESTNQ+
Sbjct: 276  SERYSSLHSISNDESMKIVRNYLIAATAKDLSMMISFRPREDGSVESPYSMVSLESTNQS 335

Query: 344  FDYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKIM 228
            FDYKA FIDLD+KPL +M  +Y+LDQ+++  Y QM K M
Sbjct: 336  FDYKAYFIDLDLKPLERMEYYYKLDQQIVGCYVQMVKSM 374


>ref|XP_007223293.1| hypothetical protein PRUPE_ppa005582mg [Prunus persica]
            gi|462420229|gb|EMJ24492.1| hypothetical protein
            PRUPE_ppa005582mg [Prunus persica]
          Length = 453

 Score =  277 bits (708), Expect = 6e-72
 Identities = 142/274 (51%), Positives = 185/274 (67%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP S+FI E N+IKR  TRF MHQ LKLHQGE+S  S Y+PLD+FSGS DRI+ A
Sbjct: 166  PKCGFLPSSKFIDEGNAIKRSITRFQMHQALKLHQGEVSEYSDYNPLDMFSGSKDRIHKA 225

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F+ PQN FR                +            R +FED +K VI    G 
Sbjct: 226  IRDLFSTPQNNFRVFLNGSQIFGGLGGGADSTNFVT------RGKFEDALKCVIQGGDGL 279

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          +  + +L+QLLEVQKLD+ DI+GAIHA YD++S+PCMVC     ++L 
Sbjct: 280  RTMSFLQLVAETVHNSGVLDQLLEVQKLDNLDIDGAIHAYYDIVSEPCMVCRAFGKEKLS 339

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
            +KY  LHS+SL+ES KIV+++LIAATAKDCS+MISFR   +GN+ SPY++LYLES+NQ F
Sbjct: 340  QKYLSLHSISLDESLKIVKDFLIAATAKDCSLMISFRPRKDGNTGSPYNNLYLESSNQFF 399

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQM 240
            DYK  FIDLD+K L KM ++YELD+K++ +Y +M
Sbjct: 400  DYKVNFIDLDLKQLKKMEEYYELDKKIVTYYNRM 433


>gb|EXC25059.1| Inositol-pentakisphosphate 2-kinase [Morus notabilis]
          Length = 618

 Score =  275 bits (704), Expect = 2e-71
 Identities = 138/274 (50%), Positives = 188/274 (68%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP SEFI E N+IKR   R+ MHQ LKLH+GEIS  S YDPLDLFSGS D+I+ A
Sbjct: 183  PKCGFLPISEFIGEGNAIKRSVPRYRMHQSLKLHEGEISAFSDYDPLDLFSGSKDKIHKA 242

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F++PQN FR                +     + +A      FE  +K VI  + G 
Sbjct: 243  IKDLFSSPQNNFRVFLDGSLIFGALGATADSTNFVIQEA------FEVALKGVIWGDQGL 296

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R++         I+ + ++++LL+VQ+LD  DIEGAIHA Y++IS+PCMVC +L+++++ 
Sbjct: 297  RTKAFLQLLSETIYTSGVMDRLLKVQELDDIDIEGAIHAYYNIISEPCMVCRDLEEEKVA 356

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             +Y+ LHS+SL+ES KIV+ +LIAATAKDCS+M+SFR   EG S S Y+S+ L+STNQ F
Sbjct: 357  HRYSSLHSISLDESLKIVKNFLIAATAKDCSLMMSFRLRKEGISESSYNSVSLKSTNQTF 416

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQM 240
            DYK CFIDLD+KPLGKM ++YE D+K++  Y +M
Sbjct: 417  DYKVCFIDLDLKPLGKMKEYYEKDKKIVSCYTRM 450


>ref|XP_004160062.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like [Cucumis sativus]
          Length = 311

 Score =  272 bits (696), Expect = 2e-70
 Identities = 145/276 (52%), Positives = 183/276 (66%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLPFS FI   N++KR  TRF MHQ LKLHQ EIS  S YDPLDLFSGS DRI  A
Sbjct: 24   PKCGFLPFSRFITHCNAVKRCMTRFRMHQALKLHQEEISEFSDYDPLDLFSGSKDRILKA 83

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F+ PQN FR                E  +V V +A      FED +K VI A+ G 
Sbjct: 84   IKDLFSTPQNNFRVFLNGSLIFGALGGSAESTDVIVGEA------FEDALKSVILADDGL 137

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
             +          ++++ +L++LLEVQKLDS DIEGAIHA YDVIS+PC+VC  L +DE L
Sbjct: 138  CTTSLLQLVTDTLYKSGVLDRLLEVQKLDSLDIEGAIHAYYDVISEPCVVCGQLNEDEEL 197

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             +YA LHSL L++S KIV+ +LIAATAKDCS+MISFR     +  S  +++ LEST Q F
Sbjct: 198  HRYASLHSLPLDQSLKIVKNFLIAATAKDCSLMISFRPREGEDWGSSNNTICLESTGQFF 257

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            DYK  FIDLD+KP+ KM ++YELD+K++  Y ++EK
Sbjct: 258  DYKVYFIDLDLKPMKKMEEYYELDKKIVSLYRKIEK 293


>ref|XP_004152686.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like [Cucumis sativus]
          Length = 453

 Score =  272 bits (696), Expect = 2e-70
 Identities = 145/276 (52%), Positives = 183/276 (66%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLPFS FI   N++KR  TRF MHQ LKLHQ EIS  S YDPLDLFSGS DRI  A
Sbjct: 166  PKCGFLPFSRFITHCNAVKRCMTRFRMHQALKLHQEEISEFSDYDPLDLFSGSKDRILKA 225

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F+ PQN FR                E  +V V +A      FED +K VI A+ G 
Sbjct: 226  IKDLFSTPQNNFRVFLNGSLIFGALGGSAESTDVIVGEA------FEDALKSVILADDGL 279

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
             +          ++++ +L++LLEVQKLDS DIEGAIHA YDVIS+PC+VC  L +DE L
Sbjct: 280  CTTSLLQLVTDTLYKSGVLDRLLEVQKLDSLDIEGAIHAYYDVISEPCVVCGQLNEDEEL 339

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             +YA LHSL L++S KIV+ +LIAATAKDCS+MISFR     +  S  +++ LEST Q F
Sbjct: 340  HRYASLHSLPLDQSLKIVKNFLIAATAKDCSLMISFRPREGEDWGSSNNTICLESTGQFF 399

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            DYK  FIDLD+KP+ KM ++YELD+K++  Y ++EK
Sbjct: 400  DYKVYFIDLDLKPMKKMEEYYELDKKIVSLYRKIEK 435


>ref|XP_002306883.1| hypothetical protein POPTR_0005s25180g [Populus trichocarpa]
            gi|222856332|gb|EEE93879.1| hypothetical protein
            POPTR_0005s25180g [Populus trichocarpa]
          Length = 435

 Score =  272 bits (696), Expect = 2e-70
 Identities = 139/274 (50%), Positives = 187/274 (68%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP S+F+AE NS+KR TTRF MHQ LKL + EIS LS+YDPLDLFSGS +RI+ A
Sbjct: 167  PKCGFLPLSKFMAEGNSVKRSTTRFRMHQILKLREQEISELSEYDPLDLFSGSKERIHKA 226

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   +  PQN FR                E     V +A      FED+++ +I AE G 
Sbjct: 227  IKDLYNTPQNNFRVFLNGSLIFGGSGGGTEKTNAVVGKA------FEDMLEGIIQAEDGL 280

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          ++ + +L++LLEVQK D+FDIEGAIHA Y+++SQPC VC  L +  L 
Sbjct: 281  RTMSFIQLVGETVYCSRVLDELLEVQKFDNFDIEGAIHAYYNIVSQPCAVCQQLDEAGLP 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             + + LHS+ ++ES KIV++YLIAATAKDCS+MISFR + +G   SPYS +YL+STNQ+F
Sbjct: 341  HRCSSLHSIHMDESLKIVKDYLIAATAKDCSLMISFRPTEDGEFGSPYSHVYLQSTNQSF 400

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQM 240
            +YK  FIDLD++PL KM  +YELD+K+L  Y+++
Sbjct: 401  NYKVNFIDLDLRPLKKMEDYYELDKKILNSYSRI 434


>ref|XP_006341967.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like isoform X2
            [Solanum tuberosum]
          Length = 372

 Score =  270 bits (689), Expect = 1e-69
 Identities = 147/277 (53%), Positives = 185/277 (66%), Gaps = 1/277 (0%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP SEFIA  NSIKR  TRF MHQ LKLHQG+IS +S YDPLDLFSGS DR++ A
Sbjct: 98   PKCGFLPLSEFIASENSIKRSVTRFKMHQALKLHQGKISEISAYDPLDLFSGSNDRVHKA 157

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F  PQN FR                +     V +       FE+ ++ VI A  G+
Sbjct: 158  IKGLFKTPQNNFRVFLNGSLILGGLGGNADATSCEVGET------FENALQCVIQAVDGQ 211

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLK-DDEL 525
            R+Q         I  + +LN++LEVQKLD+ DIEGAIHA Y+VISQPC+VC+    +D+L
Sbjct: 212  RTQCFLDLISKTICSSGLLNKVLEVQKLDNADIEGAIHAYYNVISQPCVVCNKQSAEDQL 271

Query: 524  LRKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQN 345
              +Y+ LHS+  +ES KIVR YLIAATAKD S+MISFR   +G+  SPYS + LESTNQ+
Sbjct: 272  SERYSSLHSILNDESMKIVRNYLIAATAKDLSMMISFRPREDGSVESPYSMVSLESTNQS 331

Query: 344  FDYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            FDYKA FIDLD+KPL +M  +Y+LDQ+++  Y QM K
Sbjct: 332  FDYKAYFIDLDLKPLERMEYYYKLDQQIVGCYVQMVK 368


>ref|XP_006341966.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like isoform X1
            [Solanum tuberosum]
          Length = 440

 Score =  270 bits (689), Expect = 1e-69
 Identities = 147/277 (53%), Positives = 185/277 (66%), Gaps = 1/277 (0%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP SEFIA  NSIKR  TRF MHQ LKLHQG+IS +S YDPLDLFSGS DR++ A
Sbjct: 166  PKCGFLPLSEFIASENSIKRSVTRFKMHQALKLHQGKISEISAYDPLDLFSGSNDRVHKA 225

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F  PQN FR                +     V +       FE+ ++ VI A  G+
Sbjct: 226  IKGLFKTPQNNFRVFLNGSLILGGLGGNADATSCEVGET------FENALQCVIQAVDGQ 279

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLK-DDEL 525
            R+Q         I  + +LN++LEVQKLD+ DIEGAIHA Y+VISQPC+VC+    +D+L
Sbjct: 280  RTQCFLDLISKTICSSGLLNKVLEVQKLDNADIEGAIHAYYNVISQPCVVCNKQSAEDQL 339

Query: 524  LRKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQN 345
              +Y+ LHS+  +ES KIVR YLIAATAKD S+MISFR   +G+  SPYS + LESTNQ+
Sbjct: 340  SERYSSLHSILNDESMKIVRNYLIAATAKDLSMMISFRPREDGSVESPYSMVSLESTNQS 399

Query: 344  FDYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            FDYKA FIDLD+KPL +M  +Y+LDQ+++  Y QM K
Sbjct: 400  FDYKAYFIDLDLKPLERMEYYYKLDQQIVGCYVQMVK 436


>tpg|DAA64569.1| TPA_exp: inositol-pentakisphosphate 2-kinase 555 [Ricinus communis]
          Length = 555

 Score =  268 bits (685), Expect = 3e-69
 Identities = 138/280 (49%), Positives = 184/280 (65%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGF+P S  IAE N++K+ TTRF MHQ LKL Q E S +S+YDPLD+FSGS +RI  A
Sbjct: 167  PKCGFIPLSRLIAERNALKKSTTRFKMHQVLKLRQHETSEVSQYDPLDIFSGSKERIQKA 226

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            ++  +A PQN FR                    + + Q       FED +K  I A+ G 
Sbjct: 227  ISDLYATPQNNFRVFLNGSLIFGGLGGGTNKTSIVIGQG------FEDALKGFIQADDGL 280

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          I+ +++L++LL VQKLD  DIEGAIHA Y+VISQPC+VC  L +  L 
Sbjct: 281  RTTSLIELVAEAIYSSQVLDRLLAVQKLDHLDIEGAIHAYYNVISQPCVVCRELDEAGLP 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             KY  LHS+ ++ES KIV+++LI+ATAKDCS+MISFR   + +  SPYS++YL+STNQNF
Sbjct: 341  HKYVALHSIPMDESLKIVKDFLISATAKDCSLMISFRSREDADFGSPYSNIYLQSTNQNF 400

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKIMYL 222
            DYK  F+DLD+KPL KM  +Y+ D+K+L  YAQM +  +L
Sbjct: 401  DYKVNFLDLDLKPLKKMETYYQKDKKILDSYAQMLETNHL 440


>tpg|DAA64568.1| TPA_exp: inositol-pentakisphosphate 2-kinase 452 [Ricinus communis]
          Length = 452

 Score =  268 bits (685), Expect = 3e-69
 Identities = 138/280 (49%), Positives = 184/280 (65%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGF+P S  IAE N++K+ TTRF MHQ LKL Q E S +S+YDPLD+FSGS +RI  A
Sbjct: 167  PKCGFIPLSRLIAERNALKKSTTRFKMHQVLKLRQHETSEVSQYDPLDIFSGSKERIQKA 226

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            ++  +A PQN FR                    + + Q       FED +K  I A+ G 
Sbjct: 227  ISDLYATPQNNFRVFLNGSLIFGGLGGGTNKTSIVIGQG------FEDALKGFIQADDGL 280

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          I+ +++L++LL VQKLD  DIEGAIHA Y+VISQPC+VC  L +  L 
Sbjct: 281  RTTSLIELVAEAIYSSQVLDRLLAVQKLDHLDIEGAIHAYYNVISQPCVVCRELDEAGLP 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             KY  LHS+ ++ES KIV+++LI+ATAKDCS+MISFR   + +  SPYS++YL+STNQNF
Sbjct: 341  HKYVALHSIPMDESLKIVKDFLISATAKDCSLMISFRSREDADFGSPYSNIYLQSTNQNF 400

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKIMYL 222
            DYK  F+DLD+KPL KM  +Y+ D+K+L  YAQM +  +L
Sbjct: 401  DYKVNFLDLDLKPLKKMETYYQKDKKILDSYAQMLETNHL 440


>tpg|DAA64567.1| TPA_exp: inositol-pentakisphosphate 2-kinase 450 [Ricinus communis]
          Length = 450

 Score =  268 bits (685), Expect = 3e-69
 Identities = 138/280 (49%), Positives = 184/280 (65%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGF+P S  IAE N++K+ TTRF MHQ LKL Q E S +S+YDPLD+FSGS +RI  A
Sbjct: 167  PKCGFIPLSRLIAERNALKKSTTRFKMHQVLKLRQHETSEVSQYDPLDIFSGSKERIQKA 226

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            ++  +A PQN FR                    + + Q       FED +K  I A+ G 
Sbjct: 227  ISDLYATPQNNFRVFLNGSLIFGGLGGGTNKTSIVIGQG------FEDALKGFIQADDGL 280

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          I+ +++L++LL VQKLD  DIEGAIHA Y+VISQPC+VC  L +  L 
Sbjct: 281  RTTSLIELVAEAIYSSQVLDRLLAVQKLDHLDIEGAIHAYYNVISQPCVVCRELDEAGLP 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             KY  LHS+ ++ES KIV+++LI+ATAKDCS+MISFR   + +  SPYS++YL+STNQNF
Sbjct: 341  HKYVALHSIPMDESLKIVKDFLISATAKDCSLMISFRSREDADFGSPYSNIYLQSTNQNF 400

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKIMYL 222
            DYK  F+DLD+KPL KM  +Y+ D+K+L  YAQM +  +L
Sbjct: 401  DYKVNFLDLDLKPLKKMETYYQKDKKILDSYAQMLETNHL 440


>ref|XP_002510425.1| Inositol-pentakisphosphate 2-kinase, putative [Ricinus communis]
            gi|223551126|gb|EEF52612.1| Inositol-pentakisphosphate
            2-kinase, putative [Ricinus communis]
          Length = 695

 Score =  268 bits (685), Expect = 3e-69
 Identities = 138/280 (49%), Positives = 184/280 (65%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGF+P S  IAE N++K+ TTRF MHQ LKL Q E S +S+YDPLD+FSGS +RI  A
Sbjct: 167  PKCGFIPLSRLIAERNALKKSTTRFKMHQVLKLRQHETSEVSQYDPLDIFSGSKERIQKA 226

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            ++  +A PQN FR                    + + Q       FED +K  I A+ G 
Sbjct: 227  ISDLYATPQNNFRVFLNGSLIFGGLGGGTNKTSIVIGQG------FEDALKGFIQADDGL 280

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          I+ +++L++LL VQKLD  DIEGAIHA Y+VISQPC+VC  L +  L 
Sbjct: 281  RTTSLIELVAEAIYSSQVLDRLLAVQKLDHLDIEGAIHAYYNVISQPCVVCRELDEAGLP 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
             KY  LHS+ ++ES KIV+++LI+ATAKDCS+MISFR   + +  SPYS++YL+STNQNF
Sbjct: 341  HKYVALHSIPMDESLKIVKDFLISATAKDCSLMISFRSREDADFGSPYSNIYLQSTNQNF 400

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKIMYL 222
            DYK  F+DLD+KPL KM  +Y+ D+K+L  YAQM +  +L
Sbjct: 401  DYKVNFLDLDLKPLKKMETYYQKDKKILDSYAQMLETNHL 440


>ref|XP_003523790.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like [Glycine max]
          Length = 457

 Score =  261 bits (668), Expect = 3e-67
 Identities = 138/279 (49%), Positives = 185/279 (66%), Gaps = 2/279 (0%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGF+P S FI+E  +IKRR TRF MHQ LKLHQGEIS LS+Y+PLDLFSGS +RI  A
Sbjct: 168  PKCGFIPLSRFISEGTAIKRRITRFQMHQALKLHQGEISLLSEYNPLDLFSGSKERIYKA 227

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F  PQN FR                ED    + +A      FED +K VI A++G 
Sbjct: 228  IKDLFTTPQNNFRVFMNGSLIFGGLGGGAEDTNFGIAKA------FEDALKSVIQADNGL 281

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
             ++         + ++ +L++LL+VQKLD+ DIEGAIHA YD+  Q CM+C  L +++ L
Sbjct: 282  CTENLLTIVAEAVQKSGVLDRLLDVQKLDNVDIEGAIHAYYDITHQQCMICRELSEEQ-L 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASP--YSSLYLESTNQ 348
            ++Y  LHS +L+ES +IV++YLIAATAKDCS MI FR    G+S S   YS++YLEST Q
Sbjct: 341  KRYTSLHSATLDESLRIVKDYLIAATAKDCSFMICFRPRKAGDSGSGSIYSNIYLESTKQ 400

Query: 347  NFDYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKI 231
             FD+K  FIDLD+K + KM ++YELD+K++  Y +M K+
Sbjct: 401  TFDFKVYFIDLDLKRMSKMEEYYELDKKIVSCYKEMIKM 439


>ref|XP_003528009.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like isoform X1
            [Glycine max] gi|571458694|ref|XP_006581199.1| PREDICTED:
            inositol-pentakisphosphate 2-kinase-like isoform X2
            [Glycine max] gi|571458696|ref|XP_006581200.1| PREDICTED:
            inositol-pentakisphosphate 2-kinase-like isoform X3
            [Glycine max]
          Length = 457

 Score =  259 bits (663), Expect = 1e-66
 Identities = 138/279 (49%), Positives = 185/279 (66%), Gaps = 2/279 (0%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGF+P S FI+E  +IKRR TRF MHQ LKLHQGEIS LSKY+PLDLFSGS +RI  A
Sbjct: 168  PKCGFIPLSRFISEGTAIKRRITRFQMHQALKLHQGEISLLSKYNPLDLFSGSKERIYKA 227

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F  PQN FR                ED    + +A      FE+ +K VI A++G 
Sbjct: 228  IKDLFTTPQNNFRVFMNGSLIFGGLGGGAEDTNFGIAKA------FENALKSVIQADNGL 281

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
             ++         + ++ +L++LL+VQKLD+ DIEGAIHA YD+  Q C++C  L +++ L
Sbjct: 282  CTENLLTLVAEAVKKSGVLDRLLDVQKLDNVDIEGAIHAYYDITRQQCIICRELSEEQ-L 340

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASP--YSSLYLESTNQ 348
            ++Y  LHS SL+ES +IV++YLIAATAKDCS MI FRQ   G+S S    S++YLEST Q
Sbjct: 341  KRYTSLHSASLDESLRIVKDYLIAATAKDCSFMICFRQRKAGDSGSGSICSNIYLESTKQ 400

Query: 347  NFDYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKI 231
             FD+K  FIDLD+K + KM ++YELD+K++  Y +M K+
Sbjct: 401  TFDFKVYFIDLDLKRMSKMEEYYELDKKIVSCYEEMIKM 439


>ref|XP_006473539.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like isoform X1
            [Citrus sinensis]
          Length = 442

 Score =  259 bits (661), Expect = 2e-66
 Identities = 143/276 (51%), Positives = 179/276 (64%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP S FIAE N+IKR  TRF +HQ LKL + EIS  S+YDPLDLFSGS +RI  A
Sbjct: 172  PKCGFLPISRFIAEENAIKRSVTRFQLHQALKLGEQEISERSEYDPLDLFSGSKERICKA 231

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            + A + NPQN  R               +    VAV +A      FED +K  I A++G 
Sbjct: 232  IKALYTNPQNNLRVFLNGSLIFGSLGGGMCSNSVAVGEA------FEDSLKCTIKADNGL 285

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
            R+          + +  IL++LLEVQKLD+FDIEGAIHA Y++ISQPC VC  L ++++ 
Sbjct: 286  RTDSFIQLVAETVHQAGILDRLLEVQKLDNFDIEGAIHAYYNIISQPCRVCRELDEEKVS 345

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
            R    LHS+ + ES KIV++YLIAATAKDCS+MI FR      S S Y+S+YLESTNQ F
Sbjct: 346  RLCTSLHSIPMNESLKIVKDYLIAATAKDCSLMICFRPKSNEFSGS-YNSIYLESTNQVF 404

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEK 234
            DYKA F+DLD+KP  KM +HY  D+K+   Y QM K
Sbjct: 405  DYKAYFVDLDLKPFKKMEEHYAKDKKISSCYIQMLK 440


>ref|XP_004500991.1| PREDICTED: inositol-pentakisphosphate 2-kinase-like isoform X2 [Cicer
            arietinum]
          Length = 441

 Score =  258 bits (658), Expect = 4e-66
 Identities = 137/277 (49%), Positives = 181/277 (65%)
 Frame = -3

Query: 1061 PKCGFLPFSEFIAEANSIKRRTTRFGMHQGLKLHQGEISRLSKYDPLDLFSGSLDRINHA 882
            PKCGFLP S FI+E  SIK+  TRF MHQ LKLHQGEIS LS+Y+PLDLFSGS ++I+ A
Sbjct: 159  PKCGFLPVSNFISEETSIKKSVTRFEMHQALKLHQGEISLLSEYNPLDLFSGSKEKIHKA 218

Query: 881  LTAFFANPQNYFRXXXXXXXXXXXXXXXLEDAEVAVDQANKERDEFEDIIKDVIHAEHGK 702
            +   F  PQN FR                ED    + +A      FE  +K +I ++ G 
Sbjct: 219  IKDLFTTPQNNFRVFMNGSLIFGGLGGGAEDTNFCIAKA------FEVALKSIIQSDDGL 272

Query: 701  RSQXXXXXXXXXIFRTEILNQLLEVQKLDSFDIEGAIHACYDVISQPCMVCSNLKDDELL 522
             ++           ++ +L++LLEVQKLD+ DIEGAIHA YD+  Q CMVCS L +D+  
Sbjct: 273  CTENFLTLVTEAAHKSGVLDRLLEVQKLDNVDIEGAIHAYYDITHQQCMVCSELNEDQR- 331

Query: 521  RKYAVLHSLSLEESRKIVREYLIAATAKDCSVMISFRQSLEGNSASPYSSLYLESTNQNF 342
            + Y  LHS SL+ES  IV++YLIAATAKDCS+MI FR S E  S S  +++YLEST Q F
Sbjct: 332  KIYTPLHSASLDESLTIVKDYLIAATAKDCSLMICFRPSKEEESGSVCNNVYLESTKQTF 391

Query: 341  DYKACFIDLDMKPLGKMVQHYELDQKMLKFYAQMEKI 231
            D+K  FIDLD+K + KM ++YELD+K++  Y++M K+
Sbjct: 392  DFKVYFIDLDLKRMSKMEEYYELDKKIVSCYSEMLKM 428